Carbohydrate diversity: synthesis of glycoconjugates and complex carbohydrates.

abstract：:Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understa...

journal_title：Current opinion in biotechnology

authors： Dougherty MJ,Arnold FH

更新日期：2009-08-01 00:00:00

abstract：:Nutraceuticals are important natural bioactive compounds that confer health-promoting and medical benefits to humans. Globally growing demands for value-added nutraceuticals for prevention and treatment of human diseases have rendered nutraceuticals a multi-billion dollar market. However, supply limitations and extrac...

journal_title：Current opinion in biotechnology

authors： Wang J,Guleria S,Koffas MA,Yan Y

更新日期：2016-02-01 00:00:00

abstract：:Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...

journal_title：Current opinion in biotechnology

authors： Sligar SG,Salemme FR

更新日期：1992-08-01 00:00:00

abstract：:Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound protein without using harsh or denaturing elution conditions. The development of methods to identify monoclonal antibodies that bind antigens tightly, but rele...

journal_title：Current opinion in biotechnology

authors： Burgess RR,Thompson NE

更新日期：2002-08-01 00:00:00

abstract：:Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes. The number of molecules identified and characterized, however, is in clear opposition to the number of molecules that are ente...

journal_title：Current opinion in biotechnology

authors： Maurer KH

更新日期：2004-08-01 00:00:00

abstract：:The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...

journal_title：Current opinion in biotechnology

authors： Krell T,Lacal J,Reyes-Darias JA,Jimenez-Sanchez C,Sungthong R,Ortega-Calvo JJ

更新日期：2013-06-01 00:00:00

abstract：:Diols are chemicals with two hydroxyl groups which have a wide range of appealing applications as chemicals and fuels. In particular, four diol compounds, namely 1,3-propanediol (1,3-PDO), 1,2-propanediol (1,2-PDO), 2,3-butanediol (2,3-BDO) and 1,4-butanediol (1,4-BDO) can be biotechnologically produced by direct micr...

journal_title：Current opinion in biotechnology

authors： Zeng AP,Sabra W

更新日期：2011-12-01 00:00:00

abstract：:Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UB...

journal_title：Current opinion in biotechnology

authors： Lee KH,Hamashima K,Kimoto M,Hirao I

更新日期：2018-06-01 00:00:00

abstract：:The design and construction of synthetic gene circuits with complex spatiotemporal dynamics was pioneered in bacteria, but it took almost a decade until synthetic biologists were able to construct genetic circuits with complex spatiotemporal dynamics in mammalian cells. This review highlights the most recent advances ...

journal_title：Current opinion in biotechnology

authors： Weber W,Fussenegger M

更新日期：2010-10-01 00:00:00

abstract：:Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

journal_title：Current opinion in biotechnology

authors： Wagner H,Heit A,Schmitz F,Bauer S

更新日期：2004-12-01 00:00:00

abstract：:Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

journal_title：Current opinion in biotechnology

authors： Meyer RD,Cook D

更新日期：2000-02-01 00:00:00

abstract：:Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...

journal_title：Current opinion in biotechnology

authors： Marquardt LM,Sakiyama-Elbert SE

更新日期：2013-10-01 00:00:00

abstract：:There is growing scientific evidence supported by mechanistic and clinical studies that probiotics can provide health benefits. As probiotics are highly sensitive to many environmental factors, and because the propagation of many strains of intestinal origin is not straightforward, most commercial strains are selected...

journal_title：Current opinion in biotechnology

authors： Lacroix C,Yildirim S

更新日期：2007-04-01 00:00:00

abstract：:Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and ...

journal_title：Current opinion in biotechnology

authors： Zargar A,Bailey CB,Haushalter RW,Eiben CB,Katz L,Keasling JD

更新日期：2017-06-01 00:00:00

abstract：:Understanding the hierarchical structure of proteins at their fundamental length scales is essential to get insights into their functions and roles in fundamental biological processes. Near-field scanning optical microscopy (NSOM), which overcomes the diffraction limits of conventional optics, provides a powerful anal...

journal_title：Current opinion in biotechnology

authors： Yong YC,Wang YZ,Zhong JJ

更新日期：2018-12-01 00:00:00

abstract：:With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

journal_title：Current opinion in biotechnology

authors： Karst DJ,Steinebach F,Morbidelli M

更新日期：2018-10-01 00:00:00

abstract：:Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological conditions. Abs are required for the quantification of targets, dete...

journal_title：Current opinion in biotechnology

authors： Basu K,Green EM,Cheng Y,Craik CS

更新日期：2019-12-01 00:00:00

abstract：:To mitigate some of the potentially deleterious environmental and agricultural consequences associated with current land-based-biofuel feedstocks, we propose the use of biofuels derived from aquatic microbial oxygenic photoautotrophs (AMOPs), more commonly known as cyanobacteria, algae, and diatoms. Herein we review t...

journal_title：Current opinion in biotechnology

authors： Dismukes GC,Carrieri D,Bennette N,Ananyev GM,Posewitz MC

更新日期：2008-06-01 00:00:00

abstract：:Fermentation of food components by microbes occurs both during certain food production processes and in the gastro-intestinal tract. In these processes specific compounds are produced that originate from either biotransformation reactions or biosynthesis, and that can affect the health of the consumer. In this review,...

journal_title：Current opinion in biotechnology

authors： van Hylckama Vlieg JE,Veiga P,Zhang C,Derrien M,Zhao L

更新日期：2011-04-01 00:00:00

abstract：:Many of the gene products of completely sequenced organisms are 'hypothetical' - they cannot be related to any previously characterized proteins - and so are of completely unknown function. Structural studies provide one means of obtaining functional information in these cases. A 'structural genomics' project has been...

journal_title：Current opinion in biotechnology

authors： Eisenstein E,Gilliland GL,Herzberg O,Moult J,Orban J,Poljak RJ,Banerjei L,Richardson D,Howard AJ

更新日期：2000-02-01 00:00:00

abstract：:Electron capture dissociation (ECD) is a new fragmentation technique used in Fourier transform ion cyclotron resonance mass spectrometry and is complementary to traditional tandem mass spectrometry techniques. Disulfide bonds, normally stable to vibrational excitation, are preferentially cleaved in ECD. Fragmentation ...

journal_title：Current opinion in biotechnology

authors： Zubarev RA

更新日期：2004-02-01 00:00:00

abstract：:Considerable progress has been made in understanding the relationship between local amino acid sequence and local protein structure. Recent highlights include numerous studies of the structures adopted by short peptides, new approaches to correlating sequence patterns with structure patterns, and folding simulations u...

journal_title：Current opinion in biotechnology

authors： Bystroff C,Simons KT,Han KF,Baker D

更新日期：1996-08-01 00:00:00

abstract：:Solventogenic clostridia are strictly anaerobic, endospore forming bacteria that produce a large array of primary metabolites, like butanol, by anaerobically degrading simple and complex carbohydrates, including cellulose and hemicellulose. Two genomes have been sequenced and some genetic tools have been developed, bu...

journal_title：Current opinion in biotechnology

authors： Papoutsakis ET

更新日期：2008-10-01 00:00:00

abstract：:Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of B...

journal_title：Current opinion in biotechnology

authors： Plegaria JS,Kerfeld CA

更新日期：2018-06-01 00:00:00

abstract：:Recombinant proteins that have survived the challenges of process development and clinical trials are becoming blockbuster medical products. Growth factors, enzymes and antibodies are being improved by mutational approaches, fused with other proteins, and even chemically modified in vitro. Drug development and testing...

journal_title：Current opinion in biotechnology

authors： Koths K

更新日期：1995-12-01 00:00:00

abstract：:Global aquaculture is projected to grow from 10 million to 20 million metric tons by the year 2000. To meet this projection, the aquaculture industry must bring some of its infectious disease problems under control without relying on antibiotics and chemotherapeutics. Thus, vaccines for fish and shellfish are being so...

journal_title：Current opinion in biotechnology

authors： Leong JA

更新日期：1993-06-01 00:00:00

abstract：:The increase in quality, quantity, and complexity of recombinant products heavily drives the need to predictably engineer model and complex (mammalian) cell systems. However, until recently, limited tools offered the ability to precisely manipulate their genomes, thus impeding the full potential of rational cell line ...

journal_title：Current opinion in biotechnology

authors： Cheng JK,Alper HS

更新日期：2014-12-01 00:00:00

abstract：:Saccharomyces cerevisiae has decisive advantages in industrial processes due to its tolerance to alcohols and fermentation conditions. Butanol isomers are considered as suitable fuel substitutes and valuable biomass-derived chemical building blocks. Whereas high production was achieved with bacterial systems, metaboli...

journal_title：Current opinion in biotechnology

authors： Generoso WC,Schadeweg V,Oreb M,Boles E

更新日期：2015-06-01 00:00:00

abstract：:Antisense technology is finding increasing application not only in clinical development, but also for cellular engineering. Several types of antisense methods (e.g. antisense oligonucleotides, antisense RNA and small interfering RNA) can be used to inhibit the expression of a target gene. These antisense methods are b...

journal_title：Current opinion in biotechnology

authors： Lee LK,Roth CM

更新日期：2003-10-01 00:00:00

abstract：:Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in severa...

journal_title：Current opinion in biotechnology

authors： Krishnan M,Namasivayam V,Lin R,Pal R,Burns MA

更新日期：2001-02-01 00:00:00