Abstract:
:Understanding the hierarchical structure of proteins at their fundamental length scales is essential to get insights into their functions and roles in fundamental biological processes. Near-field scanning optical microscopy (NSOM), which overcomes the diffraction limits of conventional optics, provides a powerful analytical tool to image target proteins at nanoscale resolution. Especially, by combining NSOM with infrared (IR) or Raman spectroscopy, near-field nanospectroscopic imaging of a single protein is achieved. In this review, we present the recent technical progress of NSOM setup for nanospectroscopic imaging of proteins, and its application to nanospectroscopic analysis of protein structures is highlighted and critically reviewed. Finally, current challenges and perspectives on application of NSOM in emerging areas of industrial, environmental and medical biotechnology are discussed.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Yong YC,Wang YZ,Zhong JJdoi
10.1016/j.copbio.2018.01.022subject
Has Abstractpub_date
2018-12-01 00:00:00pages
106-113eissn
0958-1669issn
1879-0429pii
S0958-1669(17)30268-9journal_volume
54pub_type
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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journal_title:Current opinion in biotechnology
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