Electrical contacting of redox proteins by nanotechnological means.

abstract：:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the p...

journal_title：Current opinion in biotechnology

authors： Gygi SP,Rist B,Aebersold R

更新日期：2000-08-01 00:00:00

abstract：:Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...

journal_title：Current opinion in biotechnology

authors： Bjork SM,Joensson HN

更新日期：2019-02-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:The development of electrospray and matrix-assisted laser desorption mass spectrometry has provided protein chemists with tools for peptide and protein structure analysis with unprecedented sensitivity and molecular weight range. The two technologies can be viewed as competitive with respect to their molecular mass de...

journal_title：Current opinion in biotechnology

authors： Lee TD,Shively JE

更新日期：1991-02-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology can also ...

journal_title：Current opinion in biotechnology

authors： Olins PO,Lee SC

更新日期：1993-10-01 00:00:00

abstract：:The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...

journal_title：Current opinion in biotechnology

authors： Luna RA,Foster JA

更新日期：2015-04-01 00:00:00

abstract：:New biological inspired methods were recently developed to recover precious metals from waste streams and to concomitantly produce palladium nanoparticles on bacteria, that is, bio-Pd. This technology offers a variety of opportunities, as the process can considered to be green, tunable, affordable and scalable. The na...

journal_title：Current opinion in biotechnology

authors： Hennebel T,De Corte S,Verstraete W,Boon N

更新日期：2012-08-01 00:00:00

abstract：:Advances in metabolic engineering have given rise to the biological production of novel fuels and chemicals, but yields are often low without significant optimization. One generalizable solution is to create a specialized organelle for the sequestration of engineered metabolic pathways. Bacterial microcompartments are...

journal_title：Current opinion in biotechnology

authors： Kim EY,Tullman-Ercek D

更新日期：2013-08-01 00:00:00

abstract：:The use of ultrafast laser pulses in surgery has allowed for unprecedented precision with minimal collateral damage to surrounding tissues. For these reasons, ultrafast laser nanosurgery, as an injury model, has gained tremendous momentum in experimental biology ranging from in vitro manipulations of subcellular struc...

journal_title：Current opinion in biotechnology

authors： Ben-Yakar A,Bourgeois F

更新日期：2009-02-01 00:00:00

abstract：:Genetic engineering for complex or combined traits requires the simultaneous expression of multiple genes, and has been considered as the bottleneck for the next generation of genetic engineering in plants. Minichromosome technology provides one solution to the stable expression and maintenance of multiple transgenes ...

journal_title：Current opinion in biotechnology

authors： Yu W,Han F,Birchler JA

更新日期：2007-10-01 00:00:00

abstract：:Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connec...

journal_title：Current opinion in biotechnology

authors： Adrian L,Marco-Urrea E

更新日期：2016-10-01 00:00:00

abstract：:Plant growth and development are tightly regulated by compounds produced in trace amounts in the plant. Besides the classical phytohormones, many plant metabolites have been described to affect plant development. Among these are several phenylpropanoids, although conclusive evidence for their bioactivity at physiologi...

journal_title：Current opinion in biotechnology

authors： Vanholme B,El Houari I,Boerjan W

更新日期：2019-04-01 00:00:00

abstract：:Obtaining an abundant supply of human cells with specific differentiated properties is a key issue in many research studies and biotechnology applications. Recent advances in this area include the finding that forced expression of the enzyme telomerase can greatly increase the proliferative potential of at least some ...

journal_title：Current opinion in biotechnology

authors： Yeager TR,Reddel RR

更新日期：1999-10-01 00:00:00

abstract：:Signal transduction is the process of converting one kind of signal or stimulus into another, with the goal of processing external or internal signals into diverse functions. In recent years, progress has been made in identifying signaling pathways, investigating cross-talk and feedbacks in signaling modules, analyzin...

journal_title：Current opinion in biotechnology

authors： Kiel C,Serrano L

更新日期：2012-06-01 00:00:00

abstract：:The use of microbial consortia for bioprocessing has been limited by our ability to reliably control community composition and function simultaneously. Recent advances in synthetic biology have enabled population-level coordination and control of ecosystem stability and dynamics. Further, new experimental and computat...

journal_title：Current opinion in biotechnology

authors： Shong J,Jimenez Diaz MR,Collins CH

更新日期：2012-10-01 00:00:00

abstract：:Global aquaculture is projected to grow from 10 million to 20 million metric tons by the year 2000. To meet this projection, the aquaculture industry must bring some of its infectious disease problems under control without relying on antibiotics and chemotherapeutics. Thus, vaccines for fish and shellfish are being so...

journal_title：Current opinion in biotechnology

authors： Leong JA

更新日期：1993-06-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...

journal_title：Current opinion in biotechnology

authors： Pais DA,Carrondo MJ,Alves PM,Teixeira AP

更新日期：2014-12-01 00:00:00

abstract：:In recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. It has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ESTs had limited impact for breeding. Until recently, the models used by ...

journal_title：Current opinion in biotechnology

authors： Dal-Bianco M,Carneiro MS,Hotta CT,Chapola RG,Hoffmann HP,Garcia AA,Souza GM

更新日期：2012-04-01 00:00:00

abstract：:Despite their central role in directing some of the most complex biological processes, carbohydrates--nature's other information carrying biopolymer--have been largely ignored as building blocks for synthetic hierarchical assemblies. The non-stoichiometric binding and astronomical diversity characteristic of carbohydr...

journal_title：Current opinion in biotechnology

authors： Han X,Zheng Y,Munro CJ,Ji Y,Braunschweig AB

更新日期：2015-08-01 00:00:00

abstract：:Characterization of the various microbial populations present in exoelectrogenic biofilms provides insight into the processes required to convert complex organic matter in wastewater streams into electrical current in bioelectrochemical systems (BESs). Analysis of the community profiles of exoelectrogenic microbial co...

journal_title：Current opinion in biotechnology

authors： Kiely PD,Regan JM,Logan BE

更新日期：2011-06-01 00:00:00

abstract：:Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent...

journal_title：Current opinion in biotechnology

authors： Stoloff DH,Wanunu M

更新日期：2013-08-01 00:00:00

abstract：:Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in severa...

journal_title：Current opinion in biotechnology

authors： Krishnan M,Namasivayam V,Lin R,Pal R,Burns MA

更新日期：2001-02-01 00:00:00

abstract：:Silk combines biological properties, such as non-toxicity and biodegradability, with physico-chemical ones, for example, mechanical strength. Base on molecular engineering, nowadays also new non-silk functions can be implemented in silk materials. Driven by rational design and ingenuity, innovative recombinant silk pr...

journal_title：Current opinion in biotechnology

authors： Saric M,Scheibel T

更新日期：2019-12-01 00:00:00

abstract：:The past year has witnessed several excellent advances in basic and applied research on nucleic acids in the environment. Improved methods for extracting nucleic acids from environmental samples have been published, as well as information on the use of reporter genes in bacteria, natural genetic transformation in soil...

journal_title：Current opinion in biotechnology

authors： Trevors JT

更新日期：1996-06-01 00:00:00

abstract：:Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

journal_title：Current opinion in biotechnology

authors： Tissier A

更新日期：2018-02-01 00:00:00

abstract：:The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...

journal_title：Current opinion in biotechnology

authors： Ogawa J,Shimizu S

更新日期：2002-08-01 00:00:00

abstract：:Among different technologies of hydrogen production, bio-hydrogen production exhibits perhaps the greatest potential to replace fossil fuels. Based on recent research on dark fermentative hydrogen production, this article reviews the following aspects towards scaled-up application of this technology: bioreactor develo...

journal_title：Current opinion in biotechnology

authors： RenNanqi,GuoWanqian,LiuBingfeng,CaoGuangli,DingJie

更新日期：2011-06-01 00:00:00