Carbohydrate nanotechnology: hierarchical assembly using nature's other information carrying biopolymers.


:Despite their central role in directing some of the most complex biological processes, carbohydrates--nature's other information carrying biopolymer--have been largely ignored as building blocks for synthetic hierarchical assemblies. The non-stoichiometric binding and astronomical diversity characteristic of carbohydrates could lead to tantalizingly complex assembly algorithms, but these attributes simultaneously increase the difficulty of preparing carbohydrate assemblies and anticipating their behavior. Convergences in biotechnology, nanotechnology, polymer chemistry, surface science, and supramolecular chemistry have led to many recent important breakthroughs in glycan microarrays and synthetic carbohydrate receptors, where the idiosyncrasies of carbohydrate structure and binding are increasingly considered. We hope to inspire more researchers to consider carbohydrate structure, diversity, and binding as attractive tools for constructing synthetic hierarchical assemblies.


Curr Opin Biotechnol


Han X,Zheng Y,Munro CJ,Ji Y,Braunschweig AB




Has Abstract


2015-08-01 00:00:00












  • Engineering and application of polymerases for synthetic genetics.

    abstract::Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nu...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Houlihan G,Arangundy-Franklin S,Holliger P

    更新日期:2017-12-01 00:00:00

  • Static and dynamic light scattering of biological macromolecules: what can we learn?

    abstract::Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Murphy RM

    更新日期:1997-02-01 00:00:00

  • Steps towards 'drop-in' biofuels: focusing on metabolic pathways.

    abstract::The past decade has witnessed rapid advance in microbial production of 'drop-in' biofuels from renewable resources. Various biosynthetic pathways have been constructed to produce biofuels with diverse structures, and multiple metabolic engineering strategies have been developed to increase biofuel titers, yields, prod...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Jiang W,Gu P,Zhang F

    更新日期:2018-10-01 00:00:00

  • Advances in gentle immunoaffinity chromatography.

    abstract::Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound protein without using harsh or denaturing elution conditions. The development of methods to identify monoclonal antibodies that bind antigens tightly, but rele...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Burgess RR,Thompson NE

    更新日期:2002-08-01 00:00:00

  • Designer microbes for biosynthesis.

    abstract::Microbes have long been adapted for the biosynthetic production of useful compounds. There is increasing demand for the rapid and cheap microbial production of diverse molecules in an industrial setting. Microbes can now be designed and engineered for a particular biosynthetic purpose, thanks to recent developments in...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Quin MB,Schmidt-Dannert C

    更新日期:2014-10-01 00:00:00

  • Stem cell niche engineering through droplet microfluidics.

    abstract::Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are bei...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Allazetta S,Lutolf MP

    更新日期:2015-12-01 00:00:00

  • DNA analysis in forensics, disease and animal/plant identification.

    abstract::During 1993, significant advances have been achieved in applications of DNA analysis to forensic science, disease assessment, and animal/plant identification. These advances include the development of simple and high sample-throughput techniques for highly informative personal identification, rapid screening for patho...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Alford RL,Caskey CT

    更新日期:1994-02-01 00:00:00

  • Advances in monitoring environmental microbes.

    abstract::Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Hazen TC,Rocha AM,Techtmann SM

    更新日期:2013-06-01 00:00:00

  • Genetic engineering for fungal and bacterial diseases.

    abstract::Significant new advances at the molecular level in the field of plant-pathogen interactions form the basis for novel transgenic approaches to crop protection. The cloning of disease resistance genes and the dissection of the signal transduction components of the hypersensitive response and systemic acquired resistance...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章


    authors: Shah DM

    更新日期:1997-04-01 00:00:00

  • Microbial biocatalyst developments to upgrade fossil fuels.

    abstract::Steady increases in the average sulfur content of petroleum and stricter environmental regulations concerning the sulfur content have promoted studies of bioprocessing to upgrade fossil fuels. Bioprocesses can potentially provide a solution to the need for improved and expanded fuel upgrading worldwide, because biopro...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kilbane JJ 2nd

    更新日期:2006-06-01 00:00:00

  • Advances in the genetics of thermophilic lactic acid bacteria.

    abstract::Molecular genetics of thermophilic lactic acid bacteria has advanced in several directions: exploitation of the milk proteins and sugars; primary and secondary metabolism; stress response; and molecular ecology of bacteria and their phages. These have singularly contributed to open new avenues of scientific interest i...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Delcour J,Ferain T,Hols P

    更新日期:2000-10-01 00:00:00

  • Strategies for extended serum half-life of protein therapeutics.

    abstract::With a growing number of protein therapeutics being developed, many of them exhibiting a short plasma half-life, half-life extension strategies find increasing attention by the biotech and pharmaceutical industry. Extension of the half-life can help to reduce the number of applications and to lower doses, thus are ben...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kontermann RE

    更新日期:2011-12-01 00:00:00

  • Road to precision: recombinase-based targeting technologies for genome engineering.

    abstract::In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing to predict the expr...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Wirth D,Gama-Norton L,Riemer P,Sandhu U,Schucht R,Hauser H

    更新日期:2007-10-01 00:00:00

  • Quality and authenticity of heterologous proteins synthesized in yeast.

    abstract::Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Eckart MR,Bussineau CM

    更新日期:1996-10-01 00:00:00

  • Brave new proteins: what evolution reveals about protein structure.

    abstract::Synthetic proteins provide important information about the principles of protein structure. They illuminate the processes of natural protein evaluation, but are not limited by these processes. Here, we review studies of several mutant proteins and discuss the general principles that can be derived from them. ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pastore A,Lesk AM

    更新日期:1991-08-01 00:00:00

  • Protein folding in vitro.

    abstract::It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kuwajima K

    更新日期:1992-10-01 00:00:00

  • Gut metabotypes govern health effects of dietary polyphenols.

    abstract::Polyphenols are thought to be responsible for some of the health effects conferred by a diet rich in fruit and vegetables. Both the formation of bioactive polyphenol-derived metabolites and the modulation of colonic microbiota contribute to these health benefits. Therefore, one cannot infer biological responses from d...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Bolca S,Van de Wiele T,Possemiers S

    更新日期:2013-04-01 00:00:00

  • Advances in the analysis of single mitochondria.

    abstract::Mitochondria are both morphologically and functionally diverse, and this variety is thought to have important biological ramifications. The development of methods to probe the properties of individual mitochondria is therefore of utmost importance. Recent advances have been made using in situ microscopy techniques and...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Fuller KM,Arriaga EA

    更新日期:2003-02-01 00:00:00

  • Engineering yeast for utilization of alternative feedstocks.

    abstract::Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xyl...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Yaguchi A,Spagnuolo M,Blenner M

    更新日期:2018-10-01 00:00:00

  • Challenges and opportunities for improving food quality and nutrition through plant biotechnology.

    abstract::Plant biotechnology has been around since the advent of humankind, resulting in tremendous improvements in plant cultivation through crop domestication, breeding and selection. The emergence of transgenic approaches involving the introduction of defined DNA sequences into plants by humans has rapidly changed the surfa...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Francis D,Finer JJ,Grotewold E

    更新日期:2017-04-01 00:00:00

  • Targets for sepsis therapies: tumor necrosis factor versus interleukin-1.

    abstract::Clinical testing of therapies for sepsis that target tumor necrosis factor and interleukin-1 is currently in progress. It is now clear from early clinical results that patients display a heterogeneous response to anti-cytokine therapies that may be related to the stage or severity of disease. Experimental results show...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Russell DA,Thompson RC

    更新日期:1993-12-01 00:00:00

  • Engineering microbial cell morphology and membrane homeostasis toward industrial applications.

    abstract::Microbial cell factory performance is significantly affected by the cell morphology and membrane homeostasis. It is important to ensure that cell factories are able to regulate cell morphology and maintain membrane homeostasis. Cell morphology can be controlled by regulating the formation of elongasomes and divisomes,...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Guo L,Pang Z,Gao C,Chen X,Liu L

    更新日期:2020-12-01 00:00:00

  • Combinatorial biocatalysis: taking the lead from nature.

    abstract::Combinatorial biocatalysis is an emerging technology in the field of drug discovery. The biocatalytic approach to combinatorial chemistry uses enzymatic, chemoenzymatic, and microbial transformations to generate libraries from lead compounds. Important recent advances in combinatorial biocatalysis include iterative de...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Altreuter DH,Clark DS

    更新日期:1999-04-01 00:00:00

  • Customised nucleic acid libraries for enhanced aptamer selection and performance.

    abstract::Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applicat...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Pfeiffer F,Rosenthal M,Siegl J,Ewers J,Mayer G

    更新日期:2017-12-01 00:00:00

  • Plant secretory structures: more than just reaction bags.

    abstract::Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Tissier A

    更新日期:2018-02-01 00:00:00

  • Microorganisms relevant to bioremediation.

    abstract::Naturally occurring microbial consortia have been utilized in a variety of bioremediation processes. Recent developments in molecular microbial ecology offer new tools that facilitate molecular analyses of microbial populations at contaminated and bioremediated sites. Information provided by such analyses aids in the ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Watanabe K

    更新日期:2001-06-01 00:00:00

  • Antibody discovery: phage display.

    abstract::Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Kretzschmar T,von Rüden T

    更新日期:2002-12-01 00:00:00

  • Tracing metabolic flux through time and space with isotope labeling experiments.

    abstract::Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activiti...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Allen DK,Young JD

    更新日期:2020-08-01 00:00:00

  • Genomic analysis.

    abstract::Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Lee PS,Lee KH

    更新日期:2000-04-01 00:00:00

  • Advances in cellulosic conversion to fuels: engineering yeasts for cellulosic bioethanol and biodiesel production.

    abstract::Cellulosic fuels are expected to have great potential industrial applications in the near future, but they still face technical challenges to become cost-competitive fuels, thus presenting many opportunities for improvement. The economical production of viable biofuels requires metabolic engineering of microbial platf...

    journal_title:Current opinion in biotechnology

    pub_type: 杂志文章,评审


    authors: Ko JK,Lee SM

    更新日期:2018-04-01 00:00:00