Biological function made crystal clear - annotation of hypothetical proteins via structural genomics.

abstract：:Compared to 'live' vaccines, the immunogenicity of 'split' vaccines based on recombinant antigen (Ag) is poor, presumably because exogeneous recombinant Ag fails to efficiently access the major histocompatibility complex (MHC) class I processing pathway needed for 'cross-presentation'. Here we discuss recent evidence ...

journal_title：Current opinion in biotechnology

authors： Wagner H,Heit A,Schmitz F,Bauer S

更新日期：2004-12-01 00:00:00

abstract：:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...

journal_title：Current opinion in biotechnology

authors： Olson DG,Sparling R,Lynd LR

更新日期：2015-06-01 00:00:00

abstract：:Ultrasonographic imaging of ovarian morphology is used widely to inform reproductive health status in women. Metabolic disturbances induced by a negative energy balance (e.g. undernutrition) or positive energy balance (e.g. overnutrition, obesity) are known to drive or exacerbate reproductive dysfunction. Whether the ...

journal_title：Current opinion in biotechnology

authors： Vanden Brink H,Pea J,Lujan ME

更新日期：2020-11-25 00:00:00

abstract：:A central challenge for medicine is to predict disease risk and treatment outcomes for individuals. But what kind of information should be used to make useful predictions in biology? One important cause of phenotypic variation is of course genetics. However genetic predictions have both practical and fundamental limit...

journal_title：Current opinion in biotechnology

authors： Burga A,Lehner B

更新日期：2013-08-01 00:00:00

abstract：:Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactio...

journal_title：Current opinion in biotechnology

authors： Jaeger KE,Eggert T

更新日期：2004-08-01 00:00:00

abstract：:Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel ...

journal_title：Current opinion in biotechnology

authors： Schneckenburger H

更新日期：2005-02-01 00:00:00

abstract：:The phytodegradation of organic compounds can take place inside the plant or within the rhizosphere of the plant. Many different compounds and classes of compounds can be removed from the environment by this method, including solvents in groundwater, petroleum and aromatic compounds in soils, and volatile compounds in...

journal_title：Current opinion in biotechnology

authors： Newman LA,Reynolds CM

更新日期：2004-06-01 00:00:00

abstract：:Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgen...

journal_title：Current opinion in biotechnology

authors： Somers DA,Makarevitch I

更新日期：2004-04-01 00:00:00

abstract：:The structure determinations of the cytochrome bc1 complex and the prokaryotic potassium channel demonstrate that a wider range of membrane proteins are now amenable to study by X-ray crystallography. Furthermore, the structures of porins and interfacial membrane proteins show that membrane structural biology is becom...

journal_title：Current opinion in biotechnology

authors： Garavito RM

更新日期：1998-08-01 00:00:00

abstract：:Much progress has been made in the development of techniques for constructing dense grids either of ligands, such as peptides and oligonucleotides, or of cloned nucleic acids. Such arrays are finding practical applications in the analysis of sequence variation and gene expression. Methods for carrying out large number...

journal_title：Current opinion in biotechnology

authors： Southern EM

更新日期：1996-02-01 00:00:00

abstract：:Various strategies have been developed to exploit plants as bioreactors for the production of pharmaceutical antibodies, to engineer antibody-mediated pathogen resistance or to alter the plant phenotype by immunomodulation. Recent research developments focus on the fine-tuning of expression systems and the detailed ch...

journal_title：Current opinion in biotechnology

authors： Stoger E,Sack M,Fischer R,Christou P

更新日期：2002-04-01 00:00:00

abstract：:Considerable progress has been made in understanding the relationship between local amino acid sequence and local protein structure. Recent highlights include numerous studies of the structures adopted by short peptides, new approaches to correlating sequence patterns with structure patterns, and folding simulations u...

journal_title：Current opinion in biotechnology

authors： Bystroff C,Simons KT,Han KF,Baker D

更新日期：1996-08-01 00:00:00

abstract：:During 1993, significant advances have been achieved in applications of DNA analysis to forensic science, disease assessment, and animal/plant identification. These advances include the development of simple and high sample-throughput techniques for highly informative personal identification, rapid screening for patho...

journal_title：Current opinion in biotechnology

authors： Alford RL,Caskey CT

更新日期：1994-02-01 00:00:00

abstract：:The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerf...

journal_title：Current opinion in biotechnology

authors： Schmid-Burgk JL

更新日期：2017-12-01 00:00:00

abstract：:Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted s...

journal_title：Current opinion in biotechnology

authors： Sayler GS,Fleming JT,Nivens DE

更新日期：2001-10-01 00:00:00

abstract：:Synthetic biology combines knowledge from various disciplines including molecular biology, engineering, mathematics and physics to design and build novel proteins, genetic circuits and metabolic networks. Early efforts aimed at altering the behavior of individual elements have now evolved to focus on the construction ...

journal_title：Current opinion in biotechnology

authors： McDaniel R,Weiss R

更新日期：2005-08-01 00:00:00

abstract：:Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A-T and G-C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UB...

journal_title：Current opinion in biotechnology

authors： Lee KH,Hamashima K,Kimoto M,Hirao I

更新日期：2018-06-01 00:00:00

abstract：:Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression. In addition, the technology provides an...

journal_title：Current opinion in biotechnology

authors： Bock R

更新日期：2007-04-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:Recombinant DNA technology allows the manipulation of the physical properties of proteins that perform electron transport and photochemical processes. Recent work is reviewed that has a potential impact on the development of molecular electronic devices, within a general framework outlining strategies for device fabri...

journal_title：Current opinion in biotechnology

authors： Sligar SG,Salemme FR

更新日期：1992-08-01 00:00:00

abstract：:Efforts to develop culture technologies capable of eliciting robust human blood stem cell growth have met with limited success. Considering that adult stem cell cultures are complex systems, comprising multiple cell types with dynamically changing intracellular signalling environments and cellular compositions, this i...

journal_title：Current opinion in biotechnology

authors： Kirouac DC,Zandstra PW

更新日期：2006-10-01 00:00:00

abstract：:Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...

journal_title：Current opinion in biotechnology

authors： Marquardt LM,Sakiyama-Elbert SE

更新日期：2013-10-01 00:00:00

abstract：:Signal transduction is the process of converting one kind of signal or stimulus into another, with the goal of processing external or internal signals into diverse functions. In recent years, progress has been made in identifying signaling pathways, investigating cross-talk and feedbacks in signaling modules, analyzin...

journal_title：Current opinion in biotechnology

authors： Kiel C,Serrano L

更新日期：2012-06-01 00:00:00

abstract：:Plant biotechnology has been around since the advent of humankind, resulting in tremendous improvements in plant cultivation through crop domestication, breeding and selection. The emergence of transgenic approaches involving the introduction of defined DNA sequences into plants by humans has rapidly changed the surfa...

journal_title：Current opinion in biotechnology

authors： Francis D,Finer JJ,Grotewold E

更新日期：2017-04-01 00:00:00

abstract：:Fc fusion proteins are molecules in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. Fc fusion proteins have some antibody-like properties such as long serum half-life and easy expression and purification, making them ...

journal_title：Current opinion in biotechnology

authors： Huang C

更新日期：2009-12-01 00:00:00

abstract：:An intense interest has grown in the unique interactions of nucleic acids with metal ions, which lead to the formation of metal-base pairs and the generation of fluorescent nanomaterials. In this review, different types of metal-base pairs, especially those formed from naturally occurring nucleosides, are described wi...

journal_title：Current opinion in biotechnology

authors： Park KS,Park HG

更新日期：2014-08-01 00:00:00

abstract：:For eukaryotic cells to function properly, they divide their intracellular space in subcellular compartments, each harboring specific metabolic activities. In recent years, it has become increasingly clear that compartmentalization of metabolic pathways is a prerequisite for certain cellular functions. This has for in...

journal_title：Current opinion in biotechnology

authors： Zecchin A,Stapor PC,Goveia J,Carmeliet P

更新日期：2015-08-01 00:00:00

abstract：:The use of ultrafast laser pulses in surgery has allowed for unprecedented precision with minimal collateral damage to surrounding tissues. For these reasons, ultrafast laser nanosurgery, as an injury model, has gained tremendous momentum in experimental biology ranging from in vitro manipulations of subcellular struc...

journal_title：Current opinion in biotechnology

authors： Ben-Yakar A,Bourgeois F

更新日期：2009-02-01 00:00:00

abstract：:Recent advances in research on natural products (of both low and high molecular weight) of plant origin and their commercial potential as medicines are discussed. Substances with immunomodulating, antiviral, antimicrobial, antiparasite, antitumor, anti-inflammatory, hypoglycemic, tranquillizer and antifeedant activiti...

journal_title：Current opinion in biotechnology

authors： Yamada H

更新日期：1991-04-01 00:00:00

abstract：:Global aquaculture is projected to grow from 10 million to 20 million metric tons by the year 2000. To meet this projection, the aquaculture industry must bring some of its infectious disease problems under control without relying on antibiotics and chemotherapeutics. Thus, vaccines for fish and shellfish are being so...

journal_title：Current opinion in biotechnology

authors： Leong JA

更新日期：1993-06-01 00:00:00