Engineering yeast for utilization of alternative feedstocks.

Abstract:

:Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xylose, acetate, aromatics, and waste lipids. Scheffersomyces stipitis was engineered to produce itaconic acid from xylose. Yarrowia lipolytica produced lipids from dilute acetate at over 100g/L. Cutaneotrichosporon oleaginosus was engineered to produce omega-3 fatty acids and recently was shown to accumulate nearly 70% lipids when grown on aromatics as a carbon source. Further improvement to toolkits for genetic engineering of non-conventional yeast will enable future development of alternative substrate conversion to biochemicals.

journal_name

Curr Opin Biotechnol

authors

Yaguchi A,Spagnuolo M,Blenner M

doi

10.1016/j.copbio.2017.12.003

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

122-129

eissn

0958-1669

issn

1879-0429

pii

S0958-1669(17)30230-6

journal_volume

53

pub_type

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