Engineering yeast for utilization of alternative feedstocks.

abstract：:As bacterial resistance to currently used antibiotics increases, so too must efforts to identify novel agents and strategies for the prevention and treatment of bacterial infection. In the past, antimicrobial drug discovery efforts have focused on eradicating infection by either cidal or static agents, resulting in cl...

journal_title：Current opinion in biotechnology

authors： Alksne LE,Projan SJ

更新日期：2000-12-01 00:00:00

abstract：:The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowled...

journal_title：Current opinion in biotechnology

authors： Lee B,Vasmatzis G

更新日期：1997-08-01 00:00:00

abstract：:The information encoded in the base sequence of DNA provides substantial structural and functional information for the instructive self-assembly of one-dimensional (1D) functional DNA nanostructures. The hybridization chain reaction (HCR) and the formation of HCR-stimulated DNAzyme nanochains are presented, as a means...

journal_title：Current opinion in biotechnology

authors： Wang F,Willner B,Willner I

更新日期：2013-08-01 00:00:00

abstract：:Estrogen receptors are multi-domain proteins that interact with other proteins and DNA to fulfil their function: the regulation of transcription. During the past 2-3 years, our understanding of this complex process has increased tremendously as crystal structures of isolated ligand-binding domains in complex with vari...

journal_title：Current opinion in biotechnology

authors： Mueller-Fahrnow A,Egner U

更新日期：1999-12-01 00:00:00

abstract：:The development of new strategies for the delivery of vaccine antigens or immune modulators to the mucosal tissue includes innovative approaches such as the use of genetically modified food microorganisms and plants. Even though the 'proof-of-concept' has recently been established for these two systems, key questions ...

journal_title：Current opinion in biotechnology

authors： Mercenier A,Wiedermann U,Breiteneder H

更新日期：2001-10-01 00:00:00

abstract：:The possibility of generating electricity with microbial fuel cells has been recognized for some time, but practical applications have been slow to develop. The recent development of a microbial fuel cell that can harvest electricity from the organic matter stored in marine sediments has demonstrated the feasibility o...

journal_title：Current opinion in biotechnology

authors： Lovley DR

更新日期：2006-06-01 00:00:00

abstract：:Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destru...

journal_title：Current opinion in biotechnology

authors： Wallace CJ

更新日期：1995-08-01 00:00:00

abstract：:Fundamental biological processes such as cell-cell communication, signal transduction, molecular transport and energy conversion are performed by membrane proteins. These important proteins are studied best in their native environment, the lipid bilayer. The atomic force microscope (AFM) is the instrument of choice to...

journal_title：Current opinion in biotechnology

authors： Fotiadis D

更新日期：2012-08-01 00:00:00

abstract：:Metabolic engineering is not another form of classic manipulation of intermediary metabolism; rather, it is the purposeful design of metabolic networks. The metabolic engineering approach examines biochemical reactions in their entirety, rather than individually, and is concerned with the construction of novel pathway...

journal_title：Current opinion in biotechnology

authors： Stephanopoulos G

更新日期：1994-02-01 00:00:00

abstract：:Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...

journal_title：Current opinion in biotechnology

authors： Marquardt LM,Sakiyama-Elbert SE

更新日期：2013-10-01 00:00:00

abstract：:Metabolic flux analysis (MFA) represents a powerful tool for systems biology research on eukaryotic cells. This review describes recent advances, the challenges as well as applications of metabolic flux analysis comprising fungi, mammalian cells and plants. While MFA is widely established and applied in microorganisms...

journal_title：Current opinion in biotechnology

authors： Niklas J,Schneider K,Heinzle E

更新日期：2010-02-01 00:00:00

abstract：:Obtaining an abundant supply of human cells with specific differentiated properties is a key issue in many research studies and biotechnology applications. Recent advances in this area include the finding that forced expression of the enzyme telomerase can greatly increase the proliferative potential of at least some ...

journal_title：Current opinion in biotechnology

authors： Yeager TR,Reddel RR

更新日期：1999-10-01 00:00:00

abstract：:The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

journal_title：Current opinion in biotechnology

authors： Tao WA,Aebersold R

更新日期：2003-02-01 00:00:00

abstract：:Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are bei...

journal_title：Current opinion in biotechnology

authors： Allazetta S,Lutolf MP

更新日期：2015-12-01 00:00:00

abstract：:Both cyanobacteria and eukaryotic microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of...

journal_title：Current opinion in biotechnology

authors： Wijffels RH,Kruse O,Hellingwerf KJ

更新日期：2013-06-01 00:00:00

abstract：:Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yie...

journal_title：Current opinion in biotechnology

authors： Wen F,Nair NU,Zhao H

更新日期：2009-08-01 00:00:00

abstract：:For eukaryotic cells to function properly, they divide their intracellular space in subcellular compartments, each harboring specific metabolic activities. In recent years, it has become increasingly clear that compartmentalization of metabolic pathways is a prerequisite for certain cellular functions. This has for in...

journal_title：Current opinion in biotechnology

authors： Zecchin A,Stapor PC,Goveia J,Carmeliet P

更新日期：2015-08-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:Redox enzymes in bioelectronic devices usually lack direct electrical contact with electrodes, owing to the spatial separation of their redox centers from the conductive surfaces by the protein shells. The reconstitution of apo-enzymes on cofactor-functionalized nanostructures associated with electrodes provides a mea...

journal_title：Current opinion in biotechnology

authors： Willner B,Katz E,Willner I

更新日期：2006-12-01 00:00:00

abstract：:Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...

journal_title：Current opinion in biotechnology

authors： Newton RJ,McClary JS

更新日期：2019-06-01 00:00:00

abstract：:Soft rot pathogens cause significant losses worldwide in fruit and vegetable production during the growing season and after harvest. These pathogens engage in molecular interactions with their plant hosts via acyl-homoserine lactone (AHL) quorum sensing, pectin metabolites, phenolic acids, and auxin. The AHL-based int...

journal_title：Current opinion in biotechnology

authors： Charkowski AO

更新日期：2009-04-01 00:00:00

abstract：:It is becoming increasingly evident that intermediates observed in protein folding in vitro may be closely related to conformational states that are important in various intracellular processes. This review focuses on recent advances in in vitro protein-folding studies with particular reference to the molten globule s...

journal_title：Current opinion in biotechnology

authors： Kuwajima K

更新日期：1992-10-01 00:00:00

abstract：:Water deficit or drought stress is a major limitation to crop production globally. Plant breeders have used a wide range of technologies to successfully breed varieties that perform well under the growth conditions for their target environments but they are always seeking new opportunities to enhance rates of genetic ...

journal_title：Current opinion in biotechnology

authors： Langridge P,Reynolds MP

更新日期：2015-04-01 00:00:00

abstract：:The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerf...

journal_title：Current opinion in biotechnology

authors： Schmid-Burgk JL

更新日期：2017-12-01 00:00:00

abstract：:Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nu...

journal_title：Current opinion in biotechnology

authors： Houlihan G,Arangundy-Franklin S,Holliger P

更新日期：2017-12-01 00:00:00

abstract：:One goal of plant genetic engineering is the manipulation of protein levels within crop plants. New insights into the ubiquitin-dependent proteolytic pathway provide potential novel ways of enhancing levels of desired proteins by synthesizing them as ubiquitin fusions, and reducing levels of undesired proteins by sele...

journal_title：Current opinion in biotechnology

authors： Hondred D,Vierstra RD

更新日期：1992-04-01 00:00:00

abstract：:Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and ...

journal_title：Current opinion in biotechnology

authors： Zargar A,Bailey CB,Haushalter RW,Eiben CB,Katz L,Keasling JD

更新日期：2017-06-01 00:00:00

abstract：:Many recent advances in bioreporter technology focus on challenges related to bioengineering, yet in many applications implementation of optical signal measurement is equally susceptible to improvement. For bioluminescent bioreporters, one area of effort lies in the development of semiconductor chip-based detector mod...

journal_title：Current opinion in biotechnology

authors： Wells M

更新日期：2006-02-01 00:00:00

abstract：:A mechanistic understanding of gene regulatory network dynamics requires quantitative single-cell data of multiple network components in response to well-defined perturbations. Recent advances in the development of fluorescent biomarkers for proteins, detection of RNA and interactions, microfluidic technology, and hig...

journal_title：Current opinion in biotechnology

authors： Selimkhanov J,Hasty J,Tsimring LS

更新日期：2012-02-01 00:00:00

abstract：:Advances in genomic analysis include improved technology for DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and ...

journal_title：Current opinion in biotechnology

authors： Lee PS,Lee KH

更新日期：2000-04-01 00:00:00