Applications of interaction traps/two-hybrid systems to biotechnology research.

abstract：:Synthetic building blocks bearing hydroxylated chiral centers are important targets for biocatalysis. Many C-C bond forming enzymes have recently been investigated for new applications and new strategies towards the synthesis of natural products and related oxygenated compounds. Several old catalysts have been studied...

journal_title：Current opinion in biotechnology

authors： Fessner WD,Helaine V

更新日期：2001-12-01 00:00:00

abstract：:Microorganisms play important roles in the environmental fate of toxic metals and radionuclides with a multiplicity of mechanisms effecting transformations between soluble and insoluble forms. These mechanisms are integral components of natural biogeochemical cycles and are of potential for both in situ and ex situ bi...

journal_title：Current opinion in biotechnology

authors： Gadd GM

更新日期：2000-06-01 00:00:00

abstract：:Thiopeptide antibiotics, which are a class of sulfur-rich and highly modified peptide natural products, exhibit a wide variety of important biological properties. These antibiotics are ribosomally synthesized and arise from post-translational modifications, exemplifying a process through which nature develops the stru...

journal_title：Current opinion in biotechnology

authors： Lin Z,He Q,Liu W

更新日期：2017-12-01 00:00:00

abstract：:Recent advancements in high-throughput large-scale analytical methods to sequence genomes of organisms, and to quantify gene expression, proteins, lipids and metabolites have changed the paradigm of metabolic modeling. The cost of data generation and analysis has decreased significantly, which has allowed exponential ...

journal_title：Current opinion in biotechnology

authors： Rai A,Saito K

更新日期：2016-02-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:Plant-derived diterpenoids are indispensable to plant development, stress-resistance and interaction with environmental microorganisms. Besides significant roles in plant fitness and adaption, many bioactivities beneficial to human beings are also found in diterpenoids from terrestrial plants. However, these high-valu...

journal_title：Current opinion in biotechnology

authors： Shao J,Sun Y,Liu H,Wang Y

更新日期：2020-10-05 00:00:00

abstract：:New technologies have allowed researchers to better design, build, and analyze complex consortia. These developments are fueling a wider implementation of consortium-based bioprocessing by leveraging synthetic biology, delivering on the field's multitudinous promises of higher efficiencies, superior resiliency, augmen...

journal_title：Current opinion in biotechnology

authors： Rapp KM,Jenkins JP,Betenbaugh MJ

更新日期：2020-12-01 00:00:00

abstract：:Bacterial cells have a limited number of resources that can be allocated for gene expression. The intracellular competition for these resources has an impact on the cell physiology. Bacteria have evolved mechanisms to optimize resource allocation in a variety of scenarios, showing a trade-off between the resources use...

journal_title：Current opinion in biotechnology

authors： Kim J,Darlington A,Salvador M,Utrilla J,Jiménez JI

更新日期：2020-04-01 00:00:00

abstract：:Heterogeneity of industrial production cultures is accepted to a certain degree; however, the underlying mechanisms are seldom perceived or included in the development of new bioprocess control strategies. Population heterogeneity and its basics, perceptible in the diverse proficiency of cells, begins with asymmetric ...

journal_title：Current opinion in biotechnology

authors： Müller S,Harms H,Bley T

更新日期：2010-02-01 00:00:00

abstract：:The gastrointestinal (GI) tract is a complex system characterized by multiple cell types with a determined architectural arrangement. Tissue engineering of the GI tract aims to reinstate the architecture and function of all structural layers. The key point for successful tissue regeneration includes the use of cells/b...

journal_title：Current opinion in biotechnology

authors： Bitar KN,Zakhem E

更新日期：2013-10-01 00:00:00

abstract：:Genetic diversity within the geobiosphere encompasses enormous sensing capabilities and many non-model bacteria are of biotechnological interest. Biosensing, or more generally inducible, systems are a vital component of metabolic engineering, as they allow tight control of gene expression as well as the basis for high...

journal_title：Current opinion in biotechnology

authors： Kim NM,Sinnott RW,Sandoval NR

更新日期：2020-08-01 00:00:00

abstract：:Wound healing can no longer be considered a generic term: it is the summation of a complex series of processes beginning with coagulation and ending in remodelling. This review, therefore, summarizes the past year's advances in growth factor research according to the roles of the individual growth factors in the speci...

journal_title：Current opinion in biotechnology

authors： Robson MC

更新日期：1991-12-01 00:00:00

abstract：:Oil prices and government mandates have catalyzed rapid growth of nonfossil transportation fuels in recent years, with a large focus on ethanol from energy crops, but the food crops used as first-generation energy crops today are not optimized for this purpose. We show that the theoretical efficiency of conversion of ...

journal_title：Current opinion in biotechnology

authors： Heaton EA,Flavell RB,Mascia PN,Thomas SR,Dohleman FG,Long SP

更新日期：2008-06-01 00:00:00

abstract：:The delineation of the beta-globin locus control region has led to a new understanding of the developmental regulation of the beta-globin gene cluster. It now seems that globin gene switching is effected through the sequential and mutually exclusive interaction of the locus control region with the embryonic, fetal and...

journal_title：Current opinion in biotechnology

authors： Enver T,Greaves DR

更新日期：1991-12-01 00:00:00

abstract：:Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

journal_title：Current opinion in biotechnology

authors： Meyer RD,Cook D

更新日期：2000-02-01 00:00:00

abstract：:Redox enzymes in bioelectronic devices usually lack direct electrical contact with electrodes, owing to the spatial separation of their redox centers from the conductive surfaces by the protein shells. The reconstitution of apo-enzymes on cofactor-functionalized nanostructures associated with electrodes provides a mea...

journal_title：Current opinion in biotechnology

authors： Willner B,Katz E,Willner I

更新日期：2006-12-01 00:00:00

abstract：:Herbicides are economically important, but the non-point pollution that they cause may disrupt the surrounding environment. Phytoremediation of herbicides has been well studied using conventional plants. Transgenic plants produced for metabolizing herbicides and long-persisting pollutants can be used for phytoremediat...

journal_title：Current opinion in biotechnology

authors： Kawahigashi H

更新日期：2009-04-01 00:00:00

abstract：:Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactio...

journal_title：Current opinion in biotechnology

authors： Jaeger KE,Eggert T

更新日期：2004-08-01 00:00:00

abstract：:Adverse environmental conditions pose serious limitations to agricultural production. Classical biotechnological approaches towards increasing abiotic stress tolerance focus on boosting plant endogenous defence mechanisms. However, overexpression of regulatory elements or effectors is usually accompanied by growth han...

journal_title：Current opinion in biotechnology

authors： Cabello JV,Lodeyro AF,Zurbriggen MD

更新日期：2014-04-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:The information encoded in the base sequence of DNA provides substantial structural and functional information for the instructive self-assembly of one-dimensional (1D) functional DNA nanostructures. The hybridization chain reaction (HCR) and the formation of HCR-stimulated DNAzyme nanochains are presented, as a means...

journal_title：Current opinion in biotechnology

authors： Wang F,Willner B,Willner I

更新日期：2013-08-01 00:00:00

abstract：:Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess developm...

journal_title：Current opinion in biotechnology

authors： Bjork SM,Joensson HN

更新日期：2019-02-01 00:00:00

abstract：:RNA enzymes or ribozymes are receiving considerable attention for their potential use as highly specific inhibitors of gene expression. From the basic science perspective, the mechanisms by which ribozymes catalyze site-specific cleavage (and in some cases ligation) reactions provide exciting and active areas of scien...

journal_title：Current opinion in biotechnology

authors： Rossi JJ

更新日期：1992-02-01 00:00:00

abstract：:The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...

journal_title：Current opinion in biotechnology

authors： Ogawa J,Shimizu S

更新日期：2002-08-01 00:00:00

abstract：:Plants have a remarkable capacity for the production of a wide range of metabolites. Much has been reported and reviewed on the diversity of these metabolites and how it is achieved, for example through the evolution of enzyme families. In comparison, relatively little is known on the extraordinary metabolic productiv...

journal_title：Current opinion in biotechnology

authors： Tissier A

更新日期：2018-02-01 00:00:00

abstract：:In recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. It has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ESTs had limited impact for breeding. Until recently, the models used by ...

journal_title：Current opinion in biotechnology

authors： Dal-Bianco M,Carneiro MS,Hotta CT,Chapola RG,Hoffmann HP,Garcia AA,Souza GM

更新日期：2012-04-01 00:00:00

abstract：:Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...

journal_title：Current opinion in biotechnology

authors： Rehemtulla A,Kaufman RJ

更新日期：1992-10-01 00:00:00

abstract：:The development of electrospray and matrix-assisted laser desorption mass spectrometry has provided protein chemists with tools for peptide and protein structure analysis with unprecedented sensitivity and molecular weight range. The two technologies can be viewed as competitive with respect to their molecular mass de...

journal_title：Current opinion in biotechnology

authors： Lee TD,Shively JE

更新日期：1991-02-01 00:00:00

abstract：:A central challenge for medicine is to predict disease risk and treatment outcomes for individuals. But what kind of information should be used to make useful predictions in biology? One important cause of phenotypic variation is of course genetics. However genetic predictions have both practical and fundamental limit...

journal_title：Current opinion in biotechnology

authors： Burga A,Lehner B

更新日期：2013-08-01 00:00:00

abstract：:Various strategies have been developed to exploit plants as bioreactors for the production of pharmaceutical antibodies, to engineer antibody-mediated pathogen resistance or to alter the plant phenotype by immunomodulation. Recent research developments focus on the fine-tuning of expression systems and the detailed ch...

journal_title：Current opinion in biotechnology

authors： Stoger E,Sack M,Fischer R,Christou P

更新日期：2002-04-01 00:00:00