Road to precision: recombinase-based targeting technologies for genome engineering.

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

journal_title：Current opinion in biotechnology

authors： Gumpert J,Hoischen C

更新日期：1998-10-01 00:00:00

abstract：:To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...

journal_title：Current opinion in biotechnology

authors： Azerad R,Buisson D

更新日期：2000-12-01 00:00:00

abstract：:Fungal bioactive polysaccharides deriving mainly from the Basidiomycetes family (and some from the Ascomycetes) and medicinal mushrooms have been well known and widely used in far Asia as part of traditional diet and medicine, and in the last decades have been the core of intense research for the understanding and the...

journal_title：Current opinion in biotechnology

authors： Giavasis I

更新日期：2014-04-01 00:00:00

abstract：:Recent field and laboratory studies have evaluated the potential for aerobic co-metabolism of chlorinated solvents. Different co-metabolic substrates and different methods of application have been tried, including growing indigenous microbes in situ, and injecting into the soil subsurface strains grown in subsurface r...

journal_title：Current opinion in biotechnology

authors： Semprini L

更新日期：1997-06-01 00:00:00

abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...

journal_title：Current opinion in biotechnology

authors： O'Malley J,Woltjen K,Kaji K

更新日期：2009-10-01 00:00:00

abstract：:Although metabolic networks can be readily reconstructed through comparative genomics, the reconstruction of regulatory networks has been hindered by the relatively low level of evolutionary conservation of their molecular components. Recent developments in experimental techniques have allowed the generation of vast a...

journal_title：Current opinion in biotechnology

authors： Herrgård MJ,Covert MW,Palsson BØ

更新日期：2004-02-01 00:00:00

abstract：:Recent advancements in high-throughput large-scale analytical methods to sequence genomes of organisms, and to quantify gene expression, proteins, lipids and metabolites have changed the paradigm of metabolic modeling. The cost of data generation and analysis has decreased significantly, which has allowed exponential ...

journal_title：Current opinion in biotechnology

authors： Rai A,Saito K

更新日期：2016-02-01 00:00:00

abstract：:The rhizosphere in terrestrial systems is the region of soil surrounding plant roots where there is increased microbial activity; in aquatic plants, this definition may be less clear because of diffusion of nutrients in water, but there is still a zone of influence by plant roots in this environment [1]. Within that z...

journal_title：Current opinion in biotechnology

authors： Stout L,Nüsslein K

更新日期：2010-06-01 00:00:00

abstract：:Summary of the in vitro data support a beneficial effect of cranberry or its proanthocyanin constituents by blocking adhesion to and biofilm formation on target tissues of pathogens. In vivo data partially support these beneficial effects. Consumption of various cranberry products benefited young and elderly females i...

journal_title：Current opinion in biotechnology

authors： Shmuely H,Ofek I,Weiss EI,Rones Z,Houri-Haddad Y

更新日期：2012-04-01 00:00:00

abstract：:Strain-specificity of probiotic effects has been a cornerstone principle of probiotic science for decades. Certainly, some important mechanisms are present in only a few probiotic strains. But scientific advances now reveal commonalities among members of certain taxonomic groups of probiotic microbes. Some clinical be...

journal_title：Current opinion in biotechnology

authors： Sanders ME,Benson A,Lebeer S,Merenstein DJ,Klaenhammer TR

更新日期：2018-02-01 00:00:00

abstract：:Laser light scattering comes in two major 'flavors': dynamic and static. This noninvasive technique provides a means for investigating key size and shape properties of macromolecules in solution. Light scattering has long been an indispensable tool to the polymer physical chemist, and is seeing increased use in explor...

journal_title：Current opinion in biotechnology

authors： Murphy RM

更新日期：1997-02-01 00:00:00

abstract：:In the past two decades, biological nanopores have been developed and explored for use in sensing applications as a result of their exquisite sensitivity and easily engineered, reproducible, and economically manufactured structures. Nanopore sensing has been shown to differentiate between highly similar analytes, meas...

journal_title：Current opinion in biotechnology

authors： Schmidt J

更新日期：2016-06-01 00:00:00

abstract：:Adaptive laboratory evolution (ALE) strategies allow for the metabolic engineering of microorganisms by combining genetic variation with the selection of beneficial mutations in an unbiased fashion. These ALE strategies have been proven highly effective in the optimization of production strains. In contrast to rationa...

journal_title：Current opinion in biotechnology

authors： Portnoy VA,Bezdan D,Zengler K

更新日期：2011-08-01 00:00:00

abstract：:Microbial communities associated with a plant host, constituting a holobiont, affect the physiology and growth of the plant via metabolites that are mainly derived from their photosynthates. The structure and function of active microbial communities that assimilate root exudates can be tracked by using stable isotope ...

journal_title：Current opinion in biotechnology

authors： Haichar FEZ,Heulin T,Guyonnet JP,Achouak W

更新日期：2016-10-01 00:00:00

abstract：:Tremendous advances in biocatalytic approaches to oligosaccharide synthesis have taken place in the past two years. The use of isolated enzymes, both glycosyltransferases and glycosidases, or engineered whole cells allows the preparation of natural oligosaccharides and analogs required for glycobiology research. ...

journal_title：Current opinion in biotechnology

authors： Palcic MM

更新日期：1999-12-01 00:00:00

abstract：:The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...

journal_title：Current opinion in biotechnology

authors： Luna RA,Foster JA

更新日期：2015-04-01 00:00:00

abstract：:The initial report on APOE as a susceptibility gene for late-onset Alzheimer's disease was presented a little more than two years ago. During the past year, several significant events have given added impetus to research into Alzheimer's. The association of increased allele frequency of APOE4 with Alzheimer's disease ...

journal_title：Current opinion in biotechnology

authors： Roses AD,Saunders AM

更新日期：1994-12-01 00:00:00

abstract：:There is growing scientific evidence supported by mechanistic and clinical studies that probiotics can provide health benefits. As probiotics are highly sensitive to many environmental factors, and because the propagation of many strains of intestinal origin is not straightforward, most commercial strains are selected...

journal_title：Current opinion in biotechnology

authors： Lacroix C,Yildirim S

更新日期：2007-04-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00

abstract：:Silk combines biological properties, such as non-toxicity and biodegradability, with physico-chemical ones, for example, mechanical strength. Base on molecular engineering, nowadays also new non-silk functions can be implemented in silk materials. Driven by rational design and ingenuity, innovative recombinant silk pr...

journal_title：Current opinion in biotechnology

authors： Saric M,Scheibel T

更新日期：2019-12-01 00:00:00

abstract：:Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and ...

journal_title：Current opinion in biotechnology

authors： Zargar A,Bailey CB,Haushalter RW,Eiben CB,Katz L,Keasling JD

更新日期：2017-06-01 00:00:00

abstract：:A growing body of evidence indicates that many cellular reactions within metabolic pathways are catalyzed not by free-floating 'soluble' enzymes, but via one or more membrane-associated multienzyme complexes. This type of macromolecular organization has important implications for the overall efficiency, specificity, a...

journal_title：Current opinion in biotechnology

authors： Conrado RJ,Varner JD,DeLisa MP

更新日期：2008-10-01 00:00:00

abstract：:Fermented foods and beverages are an integral part of the human diet globally. Understanding the microbial interactions within these fermenting ecosystems is required to deliver safe products with desirable consumer properties, and moreover, maintenance of these traditions. Effective tools are required for documentati...

journal_title：Current opinion in biotechnology

authors： van Hijum SA,Vaughan EE,Vogel RF

更新日期：2013-04-01 00:00:00

abstract：:Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...

journal_title：Current opinion in biotechnology

authors： DelRosso NV,Derr ND

更新日期：2017-08-01 00:00:00

abstract：:Redox enzymes in bioelectronic devices usually lack direct electrical contact with electrodes, owing to the spatial separation of their redox centers from the conductive surfaces by the protein shells. The reconstitution of apo-enzymes on cofactor-functionalized nanostructures associated with electrodes provides a mea...

journal_title：Current opinion in biotechnology

authors： Willner B,Katz E,Willner I

更新日期：2006-12-01 00:00:00

abstract：:The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...

journal_title：Current opinion in biotechnology

authors： Sowers KR,May HD

更新日期：2013-06-01 00:00:00

abstract：:Fatty acid oxidation pathways are attractive for metabolic engineering purposes due to their cyclic nature as well as their reactions that allow for the selective functionalization of alkyl chains. These characteristics allow for the production of various chemicals, such as alcohols, alkanes, ketones and hydroxyacids,...

journal_title：Current opinion in biotechnology

authors： Kim S,Cheong S,Chou A,Gonzalez R

更新日期：2016-12-01 00:00:00

abstract：:The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattr...

journal_title：Current opinion in biotechnology

authors： Krell T,Lacal J,Reyes-Darias JA,Jimenez-Sanchez C,Sungthong R,Ortega-Calvo JJ

更新日期：2013-06-01 00:00:00

abstract：:Many significant advances have occurred recently in the field of protein electrophoresis and related technologies. Improvements have been made in capillary electrophoresis in apparatus design, detection, and capillary modification with coatings and fillers. The transfer of proteins to a blot, the capacity to analyze a...

journal_title：Current opinion in biotechnology

authors： Zewert TE,Harrington MG

更新日期：1993-02-01 00:00:00