The microbe electric: conversion of organic matter to electricity.

abstract：:Ultrasonographic imaging of ovarian morphology is used widely to inform reproductive health status in women. Metabolic disturbances induced by a negative energy balance (e.g. undernutrition) or positive energy balance (e.g. overnutrition, obesity) are known to drive or exacerbate reproductive dysfunction. Whether the ...

journal_title：Current opinion in biotechnology

authors： Vanden Brink H,Pea J,Lujan ME

更新日期：2020-11-25 00:00:00

abstract：:RNA imaging probes help us investigate how transport and dynamics of RNA contribute to subcellular RNA localization or regulation of gene expression. Out of the plethora of strategies that have been developed to image RNA in living cells, genetically encoded probes are interesting because they can be produced by the c...

journal_title：Current opinion in biotechnology

authors： Rath AK,Rentmeister A

更新日期：2015-02-01 00:00:00

abstract：:Fermentation of syngas is a means through which unutilized organic waste streams can be converted biologically into biofuels and commodity chemicals. Despite recent advances, several issues remain which limit implementation of industrial-scale syngas fermentation processes. At the cellular level, the energy conservati...

journal_title：Current opinion in biotechnology

authors： Latif H,Zeidan AA,Nielsen AT,Zengler K

更新日期：2014-06-01 00:00:00

abstract：:The spatiotemporal patterns of ion and metabolite levels in living cells are important in understanding signal transduction and metabolite flux. Imaging approaches using genetically encoded sensors are ideal for detecting such molecule dynamics, which are hard to capture otherwise. Recent years have seen iterative imp...

journal_title：Current opinion in biotechnology

authors： Okumoto S

更新日期：2010-02-01 00:00:00

abstract：:The biosynthesis of the solvents 1-butanol and acetone is restricted to species of the genus Clostridium, a diverse group of Gram-positive, endospore forming anaerobes comprising toxin-producing strains as well as terrestrial non-pathogenic species of biotechnological impact. Among solventogenic clostridia, Clostridiu...

journal_title：Current opinion in biotechnology

authors： Lütke-Eversloh T,Bahl H

更新日期：2011-10-01 00:00:00

abstract：:Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1-200 microm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit inc...

journal_title：Current opinion in biotechnology

authors： Govardhan CP

更新日期：1999-08-01 00:00:00

abstract：:Progress in biosensors has mainly been made by the improvement of the biological components and the implementation of microsystem technologies. Enzymes are still the most appropriate recognition elements because they combine high chemical specificity and inherent biocatalytic signal amplification. A breakthrough has b...

journal_title：Current opinion in biotechnology

authors： Scheller FW,Wollenberger U,Warsinke A,Lisdat F

更新日期：2001-02-01 00:00:00

abstract：:Hydrogen is already a major commodity and process intermediate for fertilizer production, petroleum processing, and chemical synthesis. It also offers unrealized potential for energy storage. While biological production offers an expandable and sustainable source, enthusiasm has been dampened by slow research progress...

journal_title：Current opinion in biotechnology

authors： Swartz J

更新日期：2020-04-01 00:00:00

abstract：:The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

journal_title：Current opinion in biotechnology

authors： Tao WA,Aebersold R

更新日期：2003-02-01 00:00:00

abstract：:Biotechnology applications require engineering complex multi-genic traits. The lack of knowledge on the genetic basis of complex phenotypes restricts our ability to rationally engineer them. However, complex phenotypes can be engineered at the systems level, utilizing directed evolution strategies that drive whole bio...

journal_title：Current opinion in biotechnology

authors： Bassalo MC,Liu R,Gill RT

更新日期：2016-06-01 00:00:00

abstract：:Heterologous production of polyketide compounds, an important class of natural products with complex chemical structures, was first demonstrated with Streptomyces parvulus in 1984. Although Streptomyces strains are good first options for heterologous polyketide biosynthesis, their slow growth kinetics prompt other hos...

journal_title：Current opinion in biotechnology

authors： Yuzawa S,Kim W,Katz L,Keasling JD

更新日期：2012-10-01 00:00:00

abstract：:The application of microbial enzymes to large-scale organic synthesis is currently attracting much attention, and has been uniquely developed especially in Japan. The discovery of new microbial enzymes through extensive and persistent screening has brought about many new and simple routes for synthetic processes. The ...

journal_title：Current opinion in biotechnology

authors： Ogawa J,Shimizu S

更新日期：2002-08-01 00:00:00

abstract：:The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...

journal_title：Current opinion in biotechnology

authors： Luna RA,Foster JA

更新日期：2015-04-01 00:00:00

abstract：:Bacterial whole-cell biosensors are very useful for toxicity measurements of various samples. Semi-specific biosensors, containing fusions of stress-regulated promoters and reporter genes, have several advantages over the traditional, general biosensors that are based on constitutively expressed reporter genes. Furthe...

journal_title：Current opinion in biotechnology

authors： Sørensen SJ,Burmølle M,Hansen LH

更新日期：2006-02-01 00:00:00

abstract：:Both prokaryotic and eukaryotic cells respond to the accumulation of unfolded proteins by increasing the transcription of genes encoding molecular chaperones and other stress-responsive proteins. Different sets of genes are activated when particular cellular compartments are burdened with unfolded proteins. Cells thus...

journal_title：Current opinion in biotechnology

authors： McMillan DR,Gething MJ,Sambrook J

更新日期：1994-10-01 00:00:00

abstract：:The most significant feature of the current transgenic models of Alzheimer's disease continues to be the amyloid phenotype. In the past year, mice have been more extensively characterized in terms of the effect of amyloid accumulation on downstream events, such as neurodegeneration and behavioral changes, but the resu...

journal_title：Current opinion in biotechnology

authors： Duff K

更新日期：1998-12-01 00:00:00

abstract：:Estrogen receptors are multi-domain proteins that interact with other proteins and DNA to fulfil their function: the regulation of transcription. During the past 2-3 years, our understanding of this complex process has increased tremendously as crystal structures of isolated ligand-binding domains in complex with vari...

journal_title：Current opinion in biotechnology

authors： Mueller-Fahrnow A,Egner U

更新日期：1999-12-01 00:00:00

abstract：:Use of the polymerase chain reaction (PCR) has become increasingly widespread in virtually all aspects of molecular biology. Recently, novel ligation-independent methods have been developed for the cloning of DNA fragments amplified using PCR. Ligation-independent cloning utilizing the enzyme uracil DNA glycosylase (t...

journal_title：Current opinion in biotechnology

authors： Rashtchian A

更新日期：1995-02-01 00:00:00

abstract：:The increasing demand for a steady, healthy food supply requires an efficient control of the major pests and plant diseases. Current management practices are based largely on the application of synthetic pesticides. The excessive use of agrochemicals has caused serious environmental and health problems. Therefore, the...

journal_title：Current opinion in biotechnology

authors： Pérez-García A,Romero D,de Vicente A

更新日期：2011-04-01 00:00:00

abstract：:Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology can also ...

journal_title：Current opinion in biotechnology

authors： Olins PO,Lee SC

更新日期：1993-10-01 00:00:00

abstract：:Recent field and laboratory studies have evaluated the potential for aerobic co-metabolism of chlorinated solvents. Different co-metabolic substrates and different methods of application have been tried, including growing indigenous microbes in situ, and injecting into the soil subsurface strains grown in subsurface r...

journal_title：Current opinion in biotechnology

authors： Semprini L

更新日期：1997-06-01 00:00:00

abstract：:Over the review period, a significant amount of literature has been published documenting the impact of biofilms on engineered and biomedical systems. Reactor systems and analytical techniques have evolved to study the molecular chemistry and microbial ecology within biofilm layers only tens of micrometers thick, and ...

journal_title：Current opinion in biotechnology

authors： Bryers JD

更新日期：1993-04-01 00:00:00

abstract：:In the past year, significant progress in the field of gene transfer has been made possible by refinement of the technique of particle bombardment. The process has been utilized for the study of gene expression in plastids and mitochondria, the production of transgenic crop plants and gene transfer into live animals. ...

journal_title：Current opinion in biotechnology

authors： Klein TM,Fitzpatrick-McElligott S

更新日期：1993-10-01 00:00:00

abstract：:Recent advancements in high-throughput large-scale analytical methods to sequence genomes of organisms, and to quantify gene expression, proteins, lipids and metabolites have changed the paradigm of metabolic modeling. The cost of data generation and analysis has decreased significantly, which has allowed exponential ...

journal_title：Current opinion in biotechnology

authors： Rai A,Saito K

更新日期：2016-02-01 00:00:00

abstract：:Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attracti...

journal_title：Current opinion in biotechnology

authors： Przybycien TM,Pujar NS,Steele LM

更新日期：2004-10-01 00:00:00

abstract：:The removal of inorganic contaminants by plants is termed phytoextraction. Recent studies have looked at the feasibility of phytoextraction, and demonstrate that both good biomass yields and metal hyperaccumulation are required to make the process efficient. Adding chelating agents to soil to increase the bioavailabil...

journal_title：Current opinion in biotechnology

authors： McGrath SP,Zhao FJ

更新日期：2003-06-01 00:00:00

abstract：:Many significant advances have occurred recently in the field of protein electrophoresis and related technologies. Improvements have been made in capillary electrophoresis in apparatus design, detection, and capillary modification with coatings and fillers. The transfer of proteins to a blot, the capacity to analyze a...

journal_title：Current opinion in biotechnology

authors： Zewert TE,Harrington MG

更新日期：1993-02-01 00:00:00

abstract：:Studies of biological systems in which there is a direct link between the challenges faced by marine organisms and biotechnologies enable us to rationally search for active natural compounds and other novel biotechnologies. This approach is proving successful in developing new methods for the prevention of marine biof...

journal_title：Current opinion in biotechnology

authors： de Nys R,Steinberg PD

更新日期：2002-06-01 00:00:00

abstract：:The recent technological advances in next generation sequencing have brought the field closer to the goal of reconstructing all genomes within a community by presenting high throughput sequencing at much lower costs. While these next-generation sequencing technologies have allowed a massive increase in available raw s...

journal_title：Current opinion in biotechnology

authors： Scholz MB,Lo CC,Chain PS

更新日期：2012-02-01 00:00:00

abstract：:Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purpos...

journal_title：Current opinion in biotechnology

authors： DelRosso NV,Derr ND

更新日期：2017-08-01 00:00:00