Making bio-sense of toxicity: new developments in whole-cell biosensors.

abstract：:To overcome the maximum 50% yield limitation of classical resolution methods, deracemization processes involving a racemization step (dynamic resolution) or a prochiral intermediate (stereoinversion) have been developed. The use of transition metal complexes as racemizing agents, in combination with an enzymatic react...

journal_title：Current opinion in biotechnology

authors： Azerad R,Buisson D

更新日期：2000-12-01 00:00:00

abstract：:In recent years, efforts to improve sugarcane have focused on the development of biotechnology for this crop. It has become clear that sugarcane lacks tools for the biotechnological route of improvement and that the initial efforts in sequencing ESTs had limited impact for breeding. Until recently, the models used by ...

journal_title：Current opinion in biotechnology

authors： Dal-Bianco M,Carneiro MS,Hotta CT,Chapola RG,Hoffmann HP,Garcia AA,Souza GM

更新日期：2012-04-01 00:00:00

abstract：:Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified...

journal_title：Current opinion in biotechnology

authors： Friedrich MW

更新日期：2006-02-01 00:00:00

abstract：:Tremendous advances in biocatalytic approaches to oligosaccharide synthesis have taken place in the past two years. The use of isolated enzymes, both glycosyltransferases and glycosidases, or engineered whole cells allows the preparation of natural oligosaccharides and analogs required for glycobiology research. ...

journal_title：Current opinion in biotechnology

authors： Palcic MM

更新日期：1999-12-01 00:00:00

abstract：:Mismatch-repair systems have been identified in organisms ranging from Escherichia coli to humans. They can repair almost all DNA base pair mismatches as well as small insertion/deletion mismatches. Molecular and biochemical analyses have shown that the core components of eukaryotic mismatch-repair systems are highly ...

journal_title：Current opinion in biotechnology

authors： Kolodner RD,Alani E

更新日期：1994-12-01 00:00:00

abstract：:The ecophysiology of microorganisms has been at the heart of microbial ecology since its early days, but only during the past decade have methods become available for cultivation-independent, direct identification of microorganisms in complex communities and for the simultaneous investigation of their activity and sub...

journal_title：Current opinion in biotechnology

authors： Wagner M,Nielsen PH,Loy A,Nielsen JL,Daims H

更新日期：2006-02-01 00:00:00

abstract：:Recombinant DNA technology has expanded the potential of the targeted screening approach in drug discovery. The use of cloned and expressed receptors as targets for the evaluation of novel compounds can significantly simplify the structure affinity/activity process. However, the patenting of cloned receptors, as well ...

journal_title：Current opinion in biotechnology

authors： Williams M

更新日期：1993-02-01 00:00:00

abstract：:Extremolytes are small organic molecules, which protect cells under extreme, virtually inhabitable conditions. Their exceptional properties can be translated into health-promoting and therapeutic activities, which open an avenue of opportunities for the cosmetic, medical, and food industries. Supported by powerful app...

journal_title：Current opinion in biotechnology

authors： Becker J,Wittmann C

更新日期：2020-10-01 00:00:00

abstract：:Recent advances in research on natural products (of both low and high molecular weight) of plant origin and their commercial potential as medicines are discussed. Substances with immunomodulating, antiviral, antimicrobial, antiparasite, antitumor, anti-inflammatory, hypoglycemic, tranquillizer and antifeedant activiti...

journal_title：Current opinion in biotechnology

authors： Yamada H

更新日期：1991-04-01 00:00:00

abstract：:Recombinant proteins that have survived the challenges of process development and clinical trials are becoming blockbuster medical products. Growth factors, enzymes and antibodies are being improved by mutational approaches, fused with other proteins, and even chemically modified in vitro. Drug development and testing...

journal_title：Current opinion in biotechnology

authors： Koths K

更新日期：1995-12-01 00:00:00

abstract：:As bacterial resistance to currently used antibiotics increases, so too must efforts to identify novel agents and strategies for the prevention and treatment of bacterial infection. In the past, antimicrobial drug discovery efforts have focused on eradicating infection by either cidal or static agents, resulting in cl...

journal_title：Current opinion in biotechnology

authors： Alksne LE,Projan SJ

更新日期：2000-12-01 00:00:00

abstract：:Direct reprogramming of somatic cells to a pluripotent state, substantiated only three years prior, is one of the most rapidly developing areas of stem cell research. The generation of patient-derived pluripotent cells applicable to disease modelling, drug screening, toxicology tests and, ultimately, autologous cell-b...

journal_title：Current opinion in biotechnology

authors： O'Malley J,Woltjen K,Kaji K

更新日期：2009-10-01 00:00:00

abstract：:Fatty acid oxidation pathways are attractive for metabolic engineering purposes due to their cyclic nature as well as their reactions that allow for the selective functionalization of alkyl chains. These characteristics allow for the production of various chemicals, such as alcohols, alkanes, ketones and hydroxyacids,...

journal_title：Current opinion in biotechnology

authors： Kim S,Cheong S,Chou A,Gonzalez R

更新日期：2016-12-01 00:00:00

abstract：:Evidence is accumulating which suggests that the vertebrate Hox homeobox gene family plays an important role in pattern formation, particularly in the specification of regional diversity. In the last year important advances in the understanding of their regulation and function have been provided using transgenic mice....

journal_title：Current opinion in biotechnology

authors： Krumlauf R

更新日期：1991-12-01 00:00:00

abstract：:Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead...

journal_title：Current opinion in biotechnology

authors： Carrillo-Carrion C,Carril M,Parak WJ

更新日期：2017-08-01 00:00:00

abstract：:Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...

journal_title：Current opinion in biotechnology

authors： Labrou NE,Papageorgiou AC,Pavli O,Flemetakis E

更新日期：2015-04-01 00:00:00

abstract：:The field of environmental monitoring has experienced a substantial progress in the last years but still the on-site control of contaminants is an elusive problem. In addition, the growing number of pollutant sources is accompanied by an increasing need of having efficient early warning systems. Several years ago bios...

journal_title：Current opinion in biotechnology

authors： Chocarro-Ruiz B,Fernández-Gavela A,Herranz S,Lechuga LM

更新日期：2017-06-01 00:00:00

abstract：:Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted s...

journal_title：Current opinion in biotechnology

authors： Sayler GS,Fleming JT,Nivens DE

更新日期：2001-10-01 00:00:00

abstract：:Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their exper...

journal_title：Current opinion in biotechnology

authors： Vervoort Y,Linares AG,Roncoroni M,Liu C,Steensels J,Verstrepen KJ

更新日期：2017-08-01 00:00:00

abstract：:New biological inspired methods were recently developed to recover precious metals from waste streams and to concomitantly produce palladium nanoparticles on bacteria, that is, bio-Pd. This technology offers a variety of opportunities, as the process can considered to be green, tunable, affordable and scalable. The na...

journal_title：Current opinion in biotechnology

authors： Hennebel T,De Corte S,Verstraete W,Boon N

更新日期：2012-08-01 00:00:00

abstract：:Anaerobic digestion is the default process for biological conversion of residue organics to renewable energy and biofuel in the form of methane. However, its scope of application is expanding, due to availability of new technologies, and the emerging drivers of energy and nutrient conservation and recovery. Here, we o...

journal_title：Current opinion in biotechnology

authors： Batstone DJ,Virdis B

更新日期：2014-06-01 00:00:00

abstract：:Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel ...

journal_title：Current opinion in biotechnology

authors： Schneckenburger H

更新日期：2005-02-01 00:00:00

abstract：:We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...

journal_title：Current opinion in biotechnology

authors： Olson DG,Sparling R,Lynd LR

更新日期：2015-06-01 00:00:00

abstract：:The alternative sigma factor sigmaB modulates the stress response of several Gram-positive bacteria, including Bacillus subtilis and the food-borne human pathogens Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus. In all these bacteria, sigmaB is responsible for the transcription of genes that can con...

journal_title：Current opinion in biotechnology

authors： van Schaik W,Abee T

更新日期：2005-04-01 00:00:00

abstract：:Based on projections for global population growth, current techniques for improving agricultural yields will not be able to address future demands for major food crops. Improving photosynthetic efficiency by engineering carbon fixation has been identified as one of the most important approaches for increasing agricult...

journal_title：Current opinion in biotechnology

authors： Giessen TW,Silver PA

更新日期：2017-08-01 00:00:00

abstract：:Signal transduction is the process of converting one kind of signal or stimulus into another, with the goal of processing external or internal signals into diverse functions. In recent years, progress has been made in identifying signaling pathways, investigating cross-talk and feedbacks in signaling modules, analyzin...

journal_title：Current opinion in biotechnology

authors： Kiel C,Serrano L

更新日期：2012-06-01 00:00:00

abstract：:Fungal bioactive polysaccharides deriving mainly from the Basidiomycetes family (and some from the Ascomycetes) and medicinal mushrooms have been well known and widely used in far Asia as part of traditional diet and medicine, and in the last decades have been the core of intense research for the understanding and the...

journal_title：Current opinion in biotechnology

authors： Giavasis I

更新日期：2014-04-01 00:00:00

abstract：:Many recent advances in bioreporter technology focus on challenges related to bioengineering, yet in many applications implementation of optical signal measurement is equally susceptible to improvement. For bioluminescent bioreporters, one area of effort lies in the development of semiconductor chip-based detector mod...

journal_title：Current opinion in biotechnology

authors： Wells M

更新日期：2006-02-01 00:00:00

abstract：:Microbe-mediated chemical production can replace traditional fossil-dependent production and hereby ensure sustainable production of chemicals that are important for our society. Significant success in economical chemical bioproduction has been accomplished by improving the cellular properties of microbial cells throu...

journal_title：Current opinion in biotechnology

authors： Liu Y,Nielsen J

更新日期：2019-12-01 00:00:00

abstract：:Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...

journal_title：Current opinion in biotechnology

authors： Junker BH

更新日期：2014-04-01 00:00:00