Techniques for the experimental investigation of the protein corona.

abstract：:The increase in quality, quantity, and complexity of recombinant products heavily drives the need to predictably engineer model and complex (mammalian) cell systems. However, until recently, limited tools offered the ability to precisely manipulate their genomes, thus impeding the full potential of rational cell line ...

journal_title：Current opinion in biotechnology

authors： Cheng JK,Alper HS

更新日期：2014-12-01 00:00:00

abstract：:The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowled...

journal_title：Current opinion in biotechnology

authors： Lee B,Vasmatzis G

更新日期：1997-08-01 00:00:00

abstract：:The ultimate goal of genome research on the model flowering plant Arabidopsis thaliana is the identification of all of the genes and understanding their functions. A major step towards this goal, the genome sequencing project, is nearing completion; however, functional studies of newly discovered genes have not yet ke...

journal_title：Current opinion in biotechnology

authors： Parinov S,Sundaresan V

更新日期：2000-04-01 00:00:00

abstract：:Steady increases in the average sulfur content of petroleum and stricter environmental regulations concerning the sulfur content have promoted studies of bioprocessing to upgrade fossil fuels. Bioprocesses can potentially provide a solution to the need for improved and expanded fuel upgrading worldwide, because biopro...

journal_title：Current opinion in biotechnology

authors： Kilbane JJ 2nd

更新日期：2006-06-01 00:00:00

abstract：:The use of microbial consortia for bioprocessing has been limited by our ability to reliably control community composition and function simultaneously. Recent advances in synthetic biology have enabled population-level coordination and control of ecosystem stability and dynamics. Further, new experimental and computat...

journal_title：Current opinion in biotechnology

authors： Shong J,Jimenez Diaz MR,Collins CH

更新日期：2012-10-01 00:00:00

abstract：:Drought, high salinity and low temperature are major environmental factors that limit plant productivity. Plants respond and adapt to these stresses in order to survive. Signaling pathways are induced in response to environmental stress and recent molecular and genetic studies have revealed that these pathways involve...

journal_title：Current opinion in biotechnology

authors： Seki M,Kamei A,Yamaguchi-Shinozaki K,Shinozaki K

更新日期：2003-04-01 00:00:00

abstract：:Cellulosic fuels are expected to have great potential industrial applications in the near future, but they still face technical challenges to become cost-competitive fuels, thus presenting many opportunities for improvement. The economical production of viable biofuels requires metabolic engineering of microbial platf...

journal_title：Current opinion in biotechnology

authors： Ko JK,Lee SM

更新日期：2018-04-01 00:00:00

abstract：:Fungal ligninolytic enzymes have broad biotechnological applications. Particularly laccases and certain fungal class II peroxidases from white-rot basidiomycetes are considered in degradation of persistent organic pollutants. Promising processes with reusable immobilized laccases in special reactors have been develope...

journal_title：Current opinion in biotechnology

authors： Kües U

更新日期：2015-06-01 00:00:00

abstract：:With the growth of our understanding of biopharmaceutical processes, a transition from classical batch to continuous integrated manufacturing of therapeutic proteins is taking place across laboratory, clinical and commercial scales. Encouraged by regulatory authorities, this transition is favoured by new emerging tech...

journal_title：Current opinion in biotechnology

authors： Karst DJ,Steinebach F,Morbidelli M

更新日期：2018-10-01 00:00:00

abstract：:In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cell...

journal_title：Current opinion in biotechnology

authors： Gumpert J,Hoischen C

更新日期：1998-10-01 00:00:00

abstract：:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

journal_title：Current opinion in biotechnology

authors： Eckart MR,Bussineau CM

更新日期：1996-10-01 00:00:00

abstract：:The continuing expansion of protein and genome sequence databases is an opportunity to identify novel enzymes with biotechnological applications. Whether applied to enzymology, chemical biology, systems biology, and microbiology, database mining must be 'user-friendly' so that experimentalists can devise focused strat...

journal_title：Current opinion in biotechnology

authors： Zallot R,Oberg N,Gerlt JA

更新日期：2021-01-05 00:00:00

abstract：:Hydrogen is already a major commodity and process intermediate for fertilizer production, petroleum processing, and chemical synthesis. It also offers unrealized potential for energy storage. While biological production offers an expandable and sustainable source, enthusiasm has been dampened by slow research progress...

journal_title：Current opinion in biotechnology

authors： Swartz J

更新日期：2020-04-01 00:00:00

abstract：:Soft rot pathogens cause significant losses worldwide in fruit and vegetable production during the growing season and after harvest. These pathogens engage in molecular interactions with their plant hosts via acyl-homoserine lactone (AHL) quorum sensing, pectin metabolites, phenolic acids, and auxin. The AHL-based int...

journal_title：Current opinion in biotechnology

authors： Charkowski AO

更新日期：2009-04-01 00:00:00

abstract：:Metabolic flux analysis (MFA) represents a powerful tool for systems biology research on eukaryotic cells. This review describes recent advances, the challenges as well as applications of metabolic flux analysis comprising fungi, mammalian cells and plants. While MFA is widely established and applied in microorganisms...

journal_title：Current opinion in biotechnology

authors： Niklas J,Schneider K,Heinzle E

更新日期：2010-02-01 00:00:00

abstract：:Studies of biological systems in which there is a direct link between the challenges faced by marine organisms and biotechnologies enable us to rationally search for active natural compounds and other novel biotechnologies. This approach is proving successful in developing new methods for the prevention of marine biof...

journal_title：Current opinion in biotechnology

authors： de Nys R,Steinberg PD

更新日期：2002-06-01 00:00:00

abstract：:The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerf...

journal_title：Current opinion in biotechnology

authors： Schmid-Burgk JL

更新日期：2017-12-01 00:00:00

abstract：:The development of contrast agents that can be localized to a particular tissue or cellular epitope will potentially allow the noninvasive visualization and characterization of a variety of disease states. Recent advances have been made in the field of molecular imaging with magnetic resonance imaging and ultrasound a...

journal_title：Current opinion in biotechnology

authors： Morawski AM,Lanza GA,Wickline SA

更新日期：2005-02-01 00:00:00

abstract：:Synthetic biology efforts began in simple single-cell systems, which were relatively easy to manipulate genetically (Cameron et al., 2014). The field grew exponentially in the last two decades, and one of the latest frontiers are synthetic developmental programs for multicellular mammalian systems (Black et al., 2017;...

journal_title：Current opinion in biotechnology

authors： Santorelli M,Lam C,Morsut L

更新日期：2019-10-01 00:00:00

abstract：:Nutritional interventions are associated with transient deviations of homeostasis within the human body. The role of a balanced nutrition is, firstly, to ensure an adequate intake of nutrients to efficiently enable all metabolic processes and, secondly, to contribute to sustained human health. This is a new challenge ...

journal_title：Current opinion in biotechnology

authors： Schwager J,Mohajeri MH,Fowler A,Weber P

更新日期：2008-04-01 00:00:00

abstract：:Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...

journal_title：Current opinion in biotechnology

authors： Sittinger M,Hutmacher DW,Risbud MV

更新日期：2004-10-01 00:00:00

abstract：:Data visualization has developed in several directions: theoretical; methodological; and in new application areas. Advances include the development of a grammar of graphics, deeper understanding of human perception and implications for graphical layout, and better approaches to visualizing multi-dimensional data and l...

journal_title：Current opinion in biotechnology

authors： Meyer RD,Cook D

更新日期：2000-02-01 00:00:00

abstract：:Fundamental biological processes such as cell-cell communication, signal transduction, molecular transport and energy conversion are performed by membrane proteins. These important proteins are studied best in their native environment, the lipid bilayer. The atomic force microscope (AFM) is the instrument of choice to...

journal_title：Current opinion in biotechnology

authors： Fotiadis D

更新日期：2012-08-01 00:00:00

abstract：:Aptamers are short single-stranded oligo(deoxy)nucleotides that are selected to bind to target molecules with high affinity and specificity. Because of their sophisticated characteristics and versatile applicability, aptamers are thought to become universal molecular probes in biotechnological and therapeutic applicat...

journal_title：Current opinion in biotechnology

authors： Pfeiffer F,Rosenthal M,Siegl J,Ewers J,Mayer G

更新日期：2017-12-01 00:00:00

abstract：:Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the p...

journal_title：Current opinion in biotechnology

authors： Gygi SP,Rist B,Aebersold R

更新日期：2000-08-01 00:00:00

abstract：:During the past decade, the increasing knowledge of flavonoid biosynthesis and the important function of flavonoid compounds in plants and in human and animal nutrition have made the biosynthetic pathways to flavonoids and isoflavonoids excellent targets for metabolic engineering. Recent strategies have included intro...

journal_title：Current opinion in biotechnology

authors： Forkmann G,Martens S

更新日期：2001-04-01 00:00:00

abstract：:Most secretory proteins are synthesized as precursors with an amino-terminal signal peptide. Genetic identification of proteins essential for signal peptide dependent translocation to the Escherichia coli periplasm has led to the biochemical dissection of the secretion pathway. Additional mechanisms exist in Gram-nega...

journal_title：Current opinion in biotechnology

authors： Gennity JM,Inouye M

更新日期：1991-10-01 00:00:00

abstract：:The selection of an expression platform for recombinant biopharmaceuticals is often centered upon suitable product titers and critical quality attributes, including post-translational modifications. Although notable differences between microbial, yeast, plant, and mammalian host systems exist, recent advances have gre...

journal_title：Current opinion in biotechnology

authors： Meehl MA,Stadheim TA

更新日期：2014-12-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptabi...

journal_title：Current opinion in biotechnology

authors： Dahodwala H,Lee KH

更新日期：2019-12-01 00:00:00

abstract：:Plant biotechnology has been around since the advent of humankind, resulting in tremendous improvements in plant cultivation through crop domestication, breeding and selection. The emergence of transgenic approaches involving the introduction of defined DNA sequences into plants by humans has rapidly changed the surfa...

journal_title：Current opinion in biotechnology

authors： Francis D,Finer JJ,Grotewold E

更新日期：2017-04-01 00:00:00