Oxygen-tolerant hydrogenases in hydrogen-based technologies.

abstract：:Scaffolds are used in tissue engineering as a matrix for the seeding and attachment of human cells. The creation of porosity in three-dimensional (3D) structures of scaffolds plays a critical role in cell proliferation, migration, and differentiation into the specific tissue while secreting extracellular matrix compon...

journal_title：Current opinion in biotechnology

authors： Dehghani F,Annabi N

更新日期：2011-10-01 00:00:00

abstract：:The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are increasingly being applie...

journal_title：Current opinion in biotechnology

authors： Tao WA,Aebersold R

更新日期：2003-02-01 00:00:00

abstract：:The Silicon Cell initiative aims to understand cellular systems on the basis of the characteristics of their components. As a tool to achieve this, detailed kinetic models at the network reaction level are being constructed. Such detailed kinetic models are extremely useful for medical and biotechnological application...

journal_title：Current opinion in biotechnology

authors： Snoep JL

更新日期：2005-06-01 00:00:00

abstract：:The use of novel materials in the nano-scale size range for applications in devices, drugs and diagnostic agents comes with a number of new opportunities, and also serious challenges to human applications. The larger size of particulate-based agents, as compared to traditional drugs, allows for the significant advanta...

journal_title：Current opinion in biotechnology

authors： Scheinberg DA,Grimm J,Heller DA,Stater EP,Bradbury M,McDevitt MR

更新日期：2017-08-01 00:00:00

abstract：:Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound protein without using harsh or denaturing elution conditions. The development of methods to identify monoclonal antibodies that bind antigens tightly, but rele...

journal_title：Current opinion in biotechnology

authors： Burgess RR,Thompson NE

更新日期：2002-08-01 00:00:00

abstract：:Both cyanobacteria and eukaryotic microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of...

journal_title：Current opinion in biotechnology

authors： Wijffels RH,Kruse O,Hellingwerf KJ

更新日期：2013-06-01 00:00:00

abstract：:Yeast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the expression of authentic heterologous proteins of high quality in the biopharmaceutical, industrial and academic environments. There has been recent progress in characterizing and controlling the factors involved in determin...

journal_title：Current opinion in biotechnology

authors： Eckart MR,Bussineau CM

更新日期：1996-10-01 00:00:00

abstract：:Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are bei...

journal_title：Current opinion in biotechnology

authors： Allazetta S,Lutolf MP

更新日期：2015-12-01 00:00:00

abstract：:Endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. These cleavages typically occur at the dibasic amino acid residues Lys-Arg or Arg-Arg. The enzymes respon...

journal_title：Current opinion in biotechnology

authors： Rehemtulla A,Kaufman RJ

更新日期：1992-10-01 00:00:00

abstract：:Recent advancements in high-throughput large-scale analytical methods to sequence genomes of organisms, and to quantify gene expression, proteins, lipids and metabolites have changed the paradigm of metabolic modeling. The cost of data generation and analysis has decreased significantly, which has allowed exponential ...

journal_title：Current opinion in biotechnology

authors： Rai A,Saito K

更新日期：2016-02-01 00:00:00

abstract：:Realizing the economic benefits of alternative substrates for commodity chemical bioproduction typically requires significant metabolic engineering of common model organisms, such as Saccharomyces cerevisiae. A growing toolkit is enabling engineering of non-conventional yeast that have robust native metabolism for xyl...

journal_title：Current opinion in biotechnology

authors： Yaguchi A,Spagnuolo M,Blenner M

更新日期：2018-10-01 00:00:00

abstract：:Fundamental biological processes such as cell-cell communication, signal transduction, molecular transport and energy conversion are performed by membrane proteins. These important proteins are studied best in their native environment, the lipid bilayer. The atomic force microscope (AFM) is the instrument of choice to...

journal_title：Current opinion in biotechnology

authors： Fotiadis D

更新日期：2012-08-01 00:00:00

abstract：:Tremendous advances in biocatalytic approaches to oligosaccharide synthesis have taken place in the past two years. The use of isolated enzymes, both glycosyltransferases and glycosidases, or engineered whole cells allows the preparation of natural oligosaccharides and analogs required for glycobiology research. ...

journal_title：Current opinion in biotechnology

authors： Palcic MM

更新日期：1999-12-01 00:00:00

abstract：:Antibodies (Abs) are ubiquitous reagents for biological and biochemical research and are rapidly expanding into new therapeutic areas. They are one of the most important probes for determining how proteins function under normal and pathophysiological conditions. Abs are required for the quantification of targets, dete...

journal_title：Current opinion in biotechnology

authors： Basu K,Green EM,Cheng Y,Craik CS

更新日期：2019-12-01 00:00:00

abstract：:Antibacterial strategies targeting bacterial adhesion to substrates are considered a valuable alternative to traditional antibiotic therapy, in view of the great advantage they bring in combating the infectious process at the very early stage without selecting for drug resistant cells. Amongst bioactive compounds with...

journal_title：Current opinion in biotechnology

authors： Signoretto C,Canepari P,Stauder M,Vezzulli L,Pruzzo C

更新日期：2012-04-01 00:00:00

abstract：:The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerf...

journal_title：Current opinion in biotechnology

authors： Schmid-Burgk JL

更新日期：2017-12-01 00:00:00

abstract：:Patient-derived xenografts (PDXs) are obtained by transplanting fragments of a patient's tumour into immunodeficient mice. Growth and propagation of PDXs allows correlating therapeutic response in vivo with extensive, multi-dimensional molecular annotation, leading to identification of predictive biomarkers. PDXs are ...

journal_title：Current opinion in biotechnology

authors： Invrea F,Rovito R,Torchiaro E,Petti C,Isella C,Medico E

更新日期：2020-06-01 00:00:00

abstract：:The nature of DNA, the sequence of the human genome and our increased understanding of the genetic basis of many inherited and acquired disorders have made the possibility of curing diseases a reality. The modulation of a host's genome is now the ultimate goal in the treatment of genetic diseases. Historically, gene t...

journal_title：Current opinion in biotechnology

authors： Kren BT,Steer CJ

更新日期：2002-10-01 00:00:00

abstract：:NMR methods in drug discovery have traditionally been used to obtain structural information for drug targets or target-ligand complexes. Recently, it has been shown that NMR may be used as an alternative approach for identification of ligands that bind to protein drug targets, shifting the emphasis of many NMR laborat...

journal_title：Current opinion in biotechnology

authors： Moore JM

更新日期：1999-02-01 00:00:00

abstract：:The world continues to increase its energy use, brought about by an expanding population and a desire for a greater standard of living. This energy use coupled with the realization of the impact of carbon dioxide on the climate, has led us to reanalyze the potential of plant-based biofuels. Of the potential sources of...

journal_title：Current opinion in biotechnology

authors： Jones CS,Mayfield SP

更新日期：2012-06-01 00:00:00

abstract：:During the past decade, the increasing knowledge of flavonoid biosynthesis and the important function of flavonoid compounds in plants and in human and animal nutrition have made the biosynthetic pathways to flavonoids and isoflavonoids excellent targets for metabolic engineering. Recent strategies have included intro...

journal_title：Current opinion in biotechnology

authors： Forkmann G,Martens S

更新日期：2001-04-01 00:00:00

abstract：:Recent years have seen the introduction of novel techniques and applications of total internal reflection fluorescence microscopy (TIRFM). Key technical achievements include miniaturization, enhanced depth resolution, reduction of detection volumes and the combination of TIRFM with other microscopic techniques. Novel ...

journal_title：Current opinion in biotechnology

authors： Schneckenburger H

更新日期：2005-02-01 00:00:00

abstract：:Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies...

journal_title：Current opinion in biotechnology

authors： Suter B,Kittanakom S,Stagljar I

更新日期：2008-08-01 00:00:00

abstract：:Fragment-based approaches have provided a new paradigm for small-molecule drug discovery. The methodology is complementary to high-throughput screening approaches, starting from fragments of low molecular complexity and high ligand efficiency, and building up to more potent inhibitors. The approach, which depends heav...

journal_title：Current opinion in biotechnology

authors： Ciulli A,Abell C

更新日期：2007-12-01 00:00:00

abstract：:The most significant feature of the current transgenic models of Alzheimer's disease continues to be the amyloid phenotype. In the past year, mice have been more extensively characterized in terms of the effect of amyloid accumulation on downstream events, such as neurodegeneration and behavioral changes, but the resu...

journal_title：Current opinion in biotechnology

authors： Duff K

更新日期：1998-12-01 00:00:00

abstract：:Wastewater infrastructure is designed, in part, to remove microorganisms. However, many microorganisms are able to colonize infrastructure and resist treatment, resulting in an enormous flux of microorganisms to urban adjacent waters. These urban-associated microorganisms are discharged through three primary routes 1)...

journal_title：Current opinion in biotechnology

authors： Newton RJ,McClary JS

更新日期：2019-06-01 00:00:00

abstract：:The use of microbial consortia for bioprocessing has been limited by our ability to reliably control community composition and function simultaneously. Recent advances in synthetic biology have enabled population-level coordination and control of ecosystem stability and dynamics. Further, new experimental and computat...

journal_title：Current opinion in biotechnology

authors： Shong J,Jimenez Diaz MR,Collins CH

更新日期：2012-10-01 00:00:00

abstract：:Biological pulping has the potential to improve the quality of pulp, properties of paper and to reduce energy costs and environmental impact relative to traditional pulping operations. It has been suggested that energy savings alone could make the process economically viable. Other benefits include improved burst stre...

journal_title：Current opinion in biotechnology

authors： Breen A,Singleton FL

更新日期：1999-06-01 00:00:00

abstract：:Fc fusion proteins are molecules in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. Fc fusion proteins have some antibody-like properties such as long serum half-life and easy expression and purification, making them ...

journal_title：Current opinion in biotechnology

authors： Huang C

更新日期：2009-12-01 00:00:00

abstract：:Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, imp...

journal_title：Current opinion in biotechnology

authors： Sittinger M,Hutmacher DW,Risbud MV

更新日期：2004-10-01 00:00:00