Abstract:
:Microbial communities within anaerobic ecosystems have evolved to degrade and recycle carbon throughout the earth. A number of strains have been isolated from anaerobic microbial communities, which are rich in carbohydrate active enzymes (CAZymes) to liberate fermentable sugars from crude plant biomass (lignocellulose). However, natural anaerobic communities host a wealth of microbial diversity that has yet to be harnessed for biotechnological applications to hydrolyze crude biomass into sugars and value-added products. This review highlights recent advances in 'omics' techniques to sequence anaerobic microbial genomes, decipher microbial membership, and characterize CAZyme diversity in anaerobic microbiomes. With a focus on the herbivore rumen, we further discuss methods to discover new CAZymes, including those found within multi-enzyme fungal cellulosomes. Emerging techniques to characterize the interwoven metabolism and spatial interactions between anaerobes are also reviewed, which will prove critical to developing a predictive understanding of anaerobic communities to guide in microbiome engineering.
journal_name
Curr Opin Biotechnoljournal_title
Current opinion in biotechnologyauthors
Lillington SP,Leggieri PA,Heom KA,O'Malley MAdoi
10.1016/j.copbio.2019.08.015subject
Has Abstractpub_date
2020-04-01 00:00:00pages
38-47eissn
0958-1669issn
1879-0429pii
S0958-1669(19)30069-2journal_volume
62pub_type
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