abstract：:Trypanosoma brucei and other African trypanosomes are vector-borne parasites that cause substantial human suffering across sub-Saharan Africa. The T. brucei life cycle is punctuated by numerous developmental stages, each occurring in a specific environmental niche and characterized by a unique morphology, metabolism, ...

journal_title：Molecular microbiology

authors： Walsh B,Hill KL

更新日期：2021-01-12 00:00:00

abstract：:The recombination of short homologous ends in Escherichia coli has been known for 30 years, and it is often used for both site-directed mutagenesis and in vivo cloning. For cloning, a plasmid and target DNA fragments were converted into linear DNA fragments with short homologous ends, which are joined via recombinatio...

journal_title：Molecular microbiology

authors： Yang Y,Wang T,Yu Q,Liu H,Xun L,Xia Y

更新日期：2020-12-29 00:00:00

abstract：:The transmembrane protein ToxR plays a key role in the virulence expression system of Vibrio cholerae. The activity of ToxR is dependent on its periplasmic sensor domain (ToxRp) and on the inner membrane protein ToxS. Herein, we present the Nuclear Magnetic Resonance NMR solution structure of the sensory ToxRp contain...

journal_title：Molecular microbiology

authors： Gubensäk N,Wagner GE,Schrank E,Falsone FS,Berger TMI,Pavkov-Keller T,Reidl J,Zangger K

更新日期：2020-12-23 00:00:00

abstract：:Gram-negative bacteria, mitochondria, and chloroplasts all possess an outer membrane populated with a host of β-barrel outer-membrane proteins (βOMPs). These βOMPs play crucial roles in maintaining viability of their hosts, and therefore, it is essential to understand the biogenesis of this class of membrane proteins....

journal_title：Molecular microbiology

authors： Lundquist K,Billings E,Bi M,Wellnitz J,Noinaj N

更新日期：2020-12-13 00:00:00

abstract：:The cell wall is considered an essential component for bacterial survival, providing structural support, and protection from environmental insults. Under normal growth conditions, filamentous actinobacteria insert new cell wall material at the hyphal tips regulated by the coordinated activity of cytoskeletal proteins ...

journal_title：Molecular microbiology

authors： Ultee E,Zhong X,Shitut S,Briegel A,Claessen D

更新日期：2020-12-05 00:00:00

abstract：:Multidrug resistant (MDR) strains of Acinetobacter baumannii present a serious clinical challenge. The development of antibiotic resistance in this species is enabled by efflux pumps of the Resistance-Nodulation-Division (RND) superfamily of proteins creating an efficient permeability barrier for antibiotics. At least...

journal_title：Molecular microbiology

authors： Leus IV,Adamiak J,Trinh AN,Smith RD,Smith L,Richardson S,Ernst RK,Zgurskaya HI

更新日期：2020-12-01 00:00:00

abstract：:Initiation of Escherichia coli chromosome replication is controlled by the DnaA initiator protein. Both rifampicin-mediated inhibition of transcription and ppGpp-induced changes in global transcription stops replication at the level of initiation. Here, we show that continued DnaA protein synthesis allows for replicat...

journal_title：Molecular microbiology

authors： Riber L,Løbner-Olesen A

更新日期：2020-12-01 00:00:00

abstract：:Pseudomonas aeruginosa PAO1, an opportunistic human pathogen, deploys several strategies to resist antibiotics. It uses multidrug efflux pumps, including the MexAB-OprM pump, for antibiotic resistance, and it also produces hydrogen sulfide (H2 S) that provides some defense against antibiotics. MexR functions as a tran...

journal_title：Molecular microbiology

authors： Xuan G,Lü C,Xu H,Chen Z,Li K,Liu H,Liu H,Xia Y,Xun L

更新日期：2020-12-01 00:00:00

abstract：:Iron-sulfur (Fe-S) clusters are prosthetic groups on proteins that function in a range of enzymatic and electron transfer reactions. Fe-S cluster synthesis is essential for the survival of all eukaryotes. Independent Fe-S cluster biosynthesis pathways occur in the mitochondrion, plastid, and cytosolic compartments of ...

journal_title：Molecular microbiology

authors： Aw YTV,Seidi A,Hayward JA,Lee J,Makota FV,Rug M,van Dooren GG

更新日期：2020-11-22 00:00:00

abstract：:IscU is a central component of the ISC machinery and serves as a scaffold for de novo assembly of Fe-S clusters. The dedicated chaperone system composed of the Hsp70-chaperone HscA and the J-protein cochaperone HscB synergistically interacts with IscU and facilitates cluster transfer from IscU to recipient apo-protein...

journal_title：Molecular microbiology

authors： Sato S,Matsushima Y,Kanazawa M,Tanaka N,Fujishiro T,Kunichika K,Nakamura R,Tomioka H,Wada K,Takahashi Y

更新日期：2020-11-17 00:00:00

abstract：:Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot in crucifers. Our previous findings revealed that Xcc can degrade 4-hydroxybenzoic acid (4-HBA) via the β-ketoadipate pathway. This present study expands on this knowledge in several ways. First, we show that infective Xcc cells induce in sit...

journal_title：Molecular microbiology

authors： Chen B,Li RF,Zhou L,Qiu JH,Song K,Tang JL,He YW

更新日期：2020-11-01 00:00:00

abstract：:The fungus Magnaporthe oryzae causes blast, the most devastating disease of cultivated rice. After penetrating the leaf cuticle, M. oryzae grows as a biotroph in intimate contact with living rice epidermal cells before necrotic lesions develop. Biotrophic growth requires maintaining metabolic homeostasis while suppres...

journal_title：Molecular microbiology

authors： Rocha RO,Wilson RA

更新日期：2020-11-01 00:00:00

abstract：:Malaria-causing parasites are transmitted from humans to mosquitoes when developmentally arrested gametocytes are taken up by a female Anopheles during a blood meal. The changes in environment from human to mosquito activate gametogenesis, including a drop in temperature, a rise in pH, and a mosquito-derived molecule,...

journal_title：Molecular microbiology

authors： Brochet M,Balestra AC,Brusini L

更新日期：2020-10-28 00:00:00

abstract：:Translating ribosomes require elongation factor P (EF-P) to incorporate consecutive prolines (XPPX) into nascent peptide chains. The proteome of Corynebacterium glutamicum ATCC 13032 contains a total of 1,468 XPPX motifs, many of which are found in proteins involved in primary and secondary metabolism. We show here th...

journal_title：Molecular microbiology

authors： Pinheiro B,Petrov DP,Guo L,Martins GB,Bramkamp M,Jung K

更新日期：2020-10-04 00:00:00

abstract：:The reversible yeast-hyphae transition of the human fungal pathogen Candida albicans is tightly linked to its pathogenicity. In this study, we show that histone H2B mono-ubiquitination (H2Bub) at lysine 123 was maintained at a low level in the yeast state, whereas it increased significantly during yeast-to-hyphae tran...

journal_title：Molecular microbiology

authors： Zhu W,Fan X,Zhao Q,Xu Y,Wang X,Chen J

更新日期：2020-10-03 00:00:00

abstract：:In F. graminearum, the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15-acetyl deoxynivalenol. Secondary metabolites are not essential for survival, instead, they enable the pathogen to successfully infect its host...

journal_title：Molecular microbiology

authors： Shostak K,Bonner C,Sproule A,Thapa I,Shields SWJ,Blackwell B,Vierula J,Overy D,Subramaniam R

更新日期：2020-10-01 00:00:00

abstract：:The bacterium Streptomyces davaonensis produces the antibiotic roseoflavin, which is a riboflavin (vitamin B2 ) analog. The key enzyme of roseoflavin biosynthesis is the 8-demethyl-8-amino-riboflavin-5'-phosphate (AFP) synthase RosB which synthesizes AFP from riboflavin-5'-phosphate. AFP is not a substrate for the las...

journal_title：Molecular microbiology

authors： Schneider C,Konjik V,Kißling L,Mack M

更新日期：2020-10-01 00:00:00

abstract：:The endophytic fungus Epichloë festucae systemically colonizes the intercellular spaces of cool-season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes...

journal_title：Molecular microbiology

authors： Tanaka A,Kamiya S,Ozaki Y,Kameoka S,Kayano Y,Saikia S,Akano F,Uemura A,Takagi H,Terauchi R,Maruyama JI,Hammadeh HH,Fleissner A,Scott B,Takemoto D

更新日期：2020-10-01 00:00:00

abstract：:Bacteroides fragilis toxin (BFT) is a protein secreted by enterotoxigenic (ETBF) strains of B. fragilis. BFT is synthesized as a proprotein (proBFT) that is predicted to be a lipoprotein and that is cleaved into two discrete fragments by a clostripain-like protease called fragipain (Fpn). In this study, we obtained ev...

journal_title：Molecular microbiology

authors： Pierce JV,Fellows JD,Anderson DE,Bernstein HD

更新日期：2020-09-29 00:00:00

abstract：:The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is charact...

journal_title：Molecular microbiology

authors： Kamal SM,Cimdins-Ahne A,Lee C,Li F,Martín-Rodríguez AJ,Seferbekova Z,Afasizhev R,Wami HT,Katikaridis P,Meins L,Lünsdorf H,Dobrindt U,Mogk A,Römling U

更新日期：2020-09-28 00:00:00

abstract：:Gram-negative bacteria have evolved numerous pathways to secrete proteins across their complex cell envelopes. Here, we describe a protein secretion system that uses a holin membrane protein in tandem with a cell wall-editing enzyme to mediate the secretion of substrate proteins from the periplasm to the cell exterior...

journal_title：Molecular microbiology

authors： Palmer T,Finney AJ,Saha CK,Atkinson GC,Sargent F

更新日期：2020-09-04 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00

abstract：:Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways consti...

journal_title：Molecular microbiology

authors： Ortega DR,Kjaer A,Briegel A

更新日期：2020-09-01 00:00:00

abstract：:Candida albicans is an opportunistic yeast that can cause life-threatening systemic infection in immunocompromised individuals. During infections, C. albicans has to cope with genotoxic stresses generated by the host immune system. DNA-protein crosslink (DPC), the covalent linkage of proteins with DNA, is one type of ...

journal_title：Molecular microbiology

authors： Homchan A,Sukted J,Mongkolsuk S,Jeruzalmi D,Matangkasombut O,Pakotiprapha D

更新日期：2020-09-01 00:00:00

abstract：:Cells require a sensory system and a motility structure to achieve directed movement. Bacteria and archaea possess rotating filamentous motility structures that work in concert with the sensory chemotaxis system. This allows microorganisms to move along chemical gradients. The central response regulator protein CheY c...

journal_title：Molecular microbiology

authors： Li Z,Rodriguez-Franco M,Albers SV,Quax TEF

更新日期：2020-09-01 00:00:00

abstract：:Eeyarestatin 1 (ES1) is an inhibitor of endoplasmic reticulum (ER) associated protein degradation, Sec61-dependent Ca2+ homeostasis and protein translocation into the ER. Recently, evidence was presented showing that a smaller analog of ES1, ES24, targets the Sec61-translocon, and captures it in an open conformation t...

journal_title：Molecular microbiology

authors： Steenhuis M,Koningstein GM,Oswald J,Pick T,O'Keefe S,Koch HG,Cavalié A,Whitehead RC,Swanton E,High S,Luirink J

更新日期：2020-08-15 00:00:00

abstract：:5'-nucleotidases (5'-NTs) are enzymes that catalyze the hydrolysis of nucleoside monophosphates to produce nucleosides and phosphate. Since the identification of adenosine synthase A (AdsA) in Staphylococcus aureus in 2009, several other 5'-NTs have been discovered in Gram-positive cocci, mainly in streptococci. Despi...

journal_title：Molecular microbiology

authors： Soh KY,Loh JMS,Proft T

更新日期：2020-04-01 00:00:00

abstract：:The bacterial flagellar motor accommodates ten stator units around the rotor to produce large torque at high load. But when external load is low, some previous studies showed that a single stator unit can spin the rotor at the maximum speed, suggesting that the maximum speed does not depend on the number of active sta...

journal_title：Molecular microbiology

authors： Nakamura S,Hanaizumi Y,Morimoto YV,Inoue Y,Erhardt M,Minamino T,Namba K

更新日期：2020-04-01 00:00:00

abstract：:Bottlenecks reduce the size of the gene pool within populations of all life forms with implications for their subsequent survival. Here, we examine the effects of bottlenecks on bacterial commensal-pathogens during transmission between, and dissemination within, hosts. By reducing genetic diversity, bottlenecks may al...

journal_title：Molecular microbiology

authors： De Ste Croix M,Holmes J,Wanford JJ,Moxon ER,Oggioni MR,Bayliss CD

更新日期：2020-03-01 00:00:00

abstract：:Teichoic acids (TAs) are the most abundant glycopolymers in the cell wall of Listeria, an opportunistic Gram-positive pathogen that causes severe foodborne infections. Two different structural classes of Listeria TA exist: the polyribitolphosphate-based wall teichoic acid (WTA) that is covalently anchored to the pepti...

journal_title：Molecular microbiology

authors： Sumrall ET,Keller AP,Shen Y,Loessner MJ

更新日期：2020-03-01 00:00:00

abstract：:Trans-acting small regulatory RNAs (sRNAs) are key players in the regulation of gene expression in bacteria. There are hundreds of different sRNAs in a typical bacterium, which in contrast to eukaryotic microRNAs are more heterogeneous in length, sequence composition, and secondary structure. The vast majority of sRNA...

journal_title：Molecular microbiology

authors： Georg J,Lalaouna D,Hou S,Lott SC,Caldelari I,Marzi S,Hess WR,Romby P

更新日期：2020-03-01 00:00:00

abstract：:BldD generally functions as a repressor controlling morphological development of Streptomyces. In this work, evidences that BldD also activates antibiotic production are provided. In Streptomyces roseosporus (which produces daptomycin widely used for treatment of human infections), deletion of bldD notably reduced dap...

journal_title：Molecular microbiology

authors： Yan H,Lu X,Sun D,Zhuang S,Chen Q,Chen Z,Li J,Wen Y

更新日期：2020-01-01 00:00:00

abstract：:In nature, organic acids are a commonly used source of carbon and energy. Many bacteria use AMP-forming acid:CoA ligases to convert organic acids into their corresponding acyl-CoA derivatives, which can then enter metabolism. The soil environment contains a broad diversity of organic acids, so it is not surprising tha...

journal_title：Molecular microbiology

authors： Burckhardt RM,VanDrisse CM,Tucker AC,Escalante-Semerena JC

更新日期：2020-01-01 00:00:00

abstract：:The adaptation of microorganisms to different temperatures is an advantage in habitats with steadily changing conditions and raises the question about temperature sensing. Here we show that in the filamentous fungus Aspergillus nidulans, the hybrid histidine kinase TcsB and phytochrome are involved in temperature-indu...

journal_title：Molecular microbiology

authors： Yu Z,Ali A,Igbalajobi OA,Streng C,Leister K,Krauß N,Lamparter T,Fischer R

更新日期：2019-12-01 00:00:00

abstract：:The Crp/Fnr family of transcriptional regulators play central roles in transcriptional control of diverse physiological responses, and are activated by a surprising diversity of mechanisms. MrpC is a Crp/Fnr homolog that controls the Myxococcus xanthus developmental program. A long-standing model proposed that MrpC ac...

journal_title：Molecular microbiology

authors： Feeley BE,Bhardwaj V,McLaughlin PT,Diggs S,Blaha GM,Higgs PI

更新日期：2019-11-01 00:00:00

abstract：:The cell wall is a crucial structural feature in the vast majority of bacteria and comprises a covalently closed network of peptidoglycan (PG) strands. While PG synthesis is important for survival under many conditions, the cell wall is also a dynamic structure, undergoing degradation and remodeling by 'autolysins', e...

journal_title：Molecular microbiology

authors： Weaver AI,Jiménez-Ruiz V,Tallavajhala SR,Ransegnola BP,Wong KQ,Dörr T

更新日期：2019-10-01 00:00:00

abstract：:Filamentous hemagglutinin (FHA) is a critically important virulence factor produced by Bordetella species that cause respiratory infections in humans and other animals. It is also a prototypical member of the widespread two partner secretion (TPS) pathway family of proteins. First synthesized as a ~370 kDa protein cal...

journal_title：Molecular microbiology

authors： Nash ZM,Cotter PA

更新日期：2019-09-01 00:00:00

abstract：:The human fungal pathogen Candida albicans switches its morphology from yeast to hyphal forms. The morphological transition may render C. albicans virulent. Several signaling cascades, including those of the cyclic AMP-protein kinase A and mitogen-activated protein kinase pathways, are responsible for morphogenesis. I...

journal_title：Molecular microbiology

authors： Kim SW,Joo YJ,Chun YJ,Park YK,Kim J

更新日期：2019-09-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:Fungal 1,8-dihydroxynaphthalene (DHN) melanin plays important roles in UV protection, oxidative stress and pathogenesis. However, knowledge of the regulatory mechanisms of its biosynthesis is limited. Previous studies showed two transcription factors, PfmaF and PfmaH, located in the DHN melanin biosynthetic gene clust...

journal_title：Molecular microbiology

authors： Zhang P,Zhou S,Wang G,An Z,Liu X,Li K,Yin WB

更新日期：2019-08-01 00:00:00