Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis.

abstract：:A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...

journal_title：Molecular microbiology

authors： Traag BA,Ramirez-Peralta A,Wang Erickson AF,Setlow P,Losick R

更新日期：2013-07-01 00:00:00

abstract：:The colicin-like bacteriocins are potent protein antibiotics that have evolved to efficiently cross the outer membrane of Gram-negative bacteria by parasitizing nutrient uptake systems. We have structurally characterized the colicin M-like bacteriocin, pectocin M2, which is active against strains of Pectobacterium spp...

journal_title：Molecular microbiology

authors： Grinter R,Josts I,Zeth K,Roszak AW,McCaughey LC,Cogdell RJ,Milner JJ,Kelly SM,Byron O,Walker D

更新日期：2014-07-01 00:00:00

abstract：:Bacteroides fragilis toxin (BFT) is a protein secreted by enterotoxigenic (ETBF) strains of B. fragilis. BFT is synthesized as a proprotein (proBFT) that is predicted to be a lipoprotein and that is cleaved into two discrete fragments by a clostripain-like protease called fragipain (Fpn). In this study, we obtained ev...

journal_title：Molecular microbiology

authors： Pierce JV,Fellows JD,Anderson DE,Bernstein HD

更新日期：2020-09-29 00:00:00

abstract：:The ro mutants of Neurospora crassa are defective in nuclear migration and hyphal morphogenesis. Several of the ro loci have recently been shown to encode components of the dynein/dynactin motor complex. Here we report on the cloning and characterization of the ro-2 gene which codes for a novel 80 kDa protein that has...

journal_title：Molecular microbiology

authors： Vierula PJ,Mais JM

更新日期：1997-04-01 00:00:00

abstract：:Entry of Listeria monocytogenes into cultured epithelial cells requires production of internalin, a protein with features characteristic of some Gram-positive bacterial surface proteins, in particular an LPXTG motif preceding a hydrophobic sequence and a few basic residues at its C-terminal end. By immunofluorescence ...

journal_title：Molecular microbiology

authors： Lebrun M,Mengaud J,Ohayon H,Nato F,Cossart P

更新日期：1996-08-01 00:00:00

abstract：:Type II restriction enzymes are paired with modification enzymes that protect type II restriction sites from cleavage by methylating them. A plasmid carrying a type II restriction-modification gene complex is not easily replaced by an incompatible plasmid because loss of the former leads to cell death through chromoso...

journal_title：Molecular microbiology

authors： Handa N,Nakayama Y,Sadykov M,Kobayashi I

更新日期：2001-05-01 00:00:00

abstract：:The Escherichia coli nusG gene product is required for transcription termination by phage HK022 Nun protein at the lambda nutR site in vivo. We show that it is also essential for Nun termination at lambda nutL. Three recessive mis-sense nusG mutations have been isolated that inhibit termination by Nun at lambda nutR. ...

journal_title：Molecular microbiology

authors： Burova E,Hung SC,Chen J,Court DL,Zhou JG,Mogilnitskiy G,Gottesman ME

更新日期：1999-03-01 00:00:00

abstract：:A system where archaeal gene expression could be controlled by simple manipulation of growth conditions would enable the construction of conditional lethal mutants in essential genes, and permit the controlled overproduction of proteins in their native host. As tools for the genetic manipulation of Haloferax volcanii ...

journal_title：Molecular microbiology

authors： Large A,Stamme C,Lange C,Duan Z,Allers T,Soppa J,Lund PA

更新日期：2007-12-01 00:00:00

abstract：:While Vipp1 (also known as IM30) clearly is essential for proper biogenesis of thylakoid membranes in chloroplasts and cyanobacteria, the exact function of Vipp1/IM30 still remains unclear. The recent in vivo study of Gutu et al. now demonstrates that Vipp1/IM30 forms localized puncta specifically at highly curved mem...

journal_title：Molecular microbiology

authors： Junglas B,Schneider D

更新日期：2018-04-01 00:00:00

abstract：:Deletion of gene Rv3676 in Mycobacterium tuberculosis coding for a transcription factor belonging to the cAMP receptor protein (CRP) family caused growth defects in laboratory medium, in bone marrow-derived macrophages and in a mouse model of tuberculosis. Transcript profiling of M. tuberculosis grown in vitro identif...

journal_title：Molecular microbiology

authors： Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

更新日期：2005-06-01 00:00:00

abstract：:Despite their high genomic synteny, the Leishmania major and Leishmania infantum species exhibit extensive differences in mRNA expression patterns throughout the parasite's development. Yet, the underlying mechanisms for this species-specific differential gene expression are largely unknown. Here we report that Short ...

journal_title：Molecular microbiology

authors： Müller M,Padmanabhan PK,Papadopoulou B

更新日期：2010-07-01 00:00:00

abstract：:A Lactobacillus casei BL23 strain defective in an OmpR-family response regulator encoded by LCABL_18980 (PrcR, RR11), showed enhanced proteolytic activity caused by overexpression of the gene encoding the proteinase PrtP. Transcriptomic analysis revealed that, in addition to prtP expression, PrcR regulates genes encod...

journal_title：Molecular microbiology

authors： Alcántara C,Bäuerl C,Revilla-Guarinos A,Pérez-Martínez G,Monedero V,Zúñiga M

更新日期：2016-04-01 00:00:00

abstract：:LamB glycoporin has an important general role in carbohydrate uptake during growth at low extracellular sugar concentrations. lamB and mal regulon induction during glucose starvation and glucose-limited continuous culture was investigated using lacZ fusions. A low-level burst of lamB induction occurred upon entry into...

journal_title：Molecular microbiology

authors： Notley L,Ferenci T

更新日期：1995-04-01 00:00:00

abstract：:Malaria parasites scavenge nutrients from their host but also harbour enzymatic pathways for de novo macromolecule synthesis. One such pathway is apicoplast-targeted type II fatty acid synthesis, which is essential for late liver-stage development in rodent malaria. It is likely that fatty acids synthesized in the api...

journal_title：Molecular microbiology

authors： Lindner SE,Sartain MJ,Hayes K,Harupa A,Moritz RL,Kappe SH,Vaughan AM

更新日期：2014-02-01 00:00:00

abstract：:The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family of antigenically diverse proteins is expressed on the surface of human erythrocytes infected with the malaria parasite P. falciparum, and mediates cytoadherence to the host vascular endothelium. In this report, we show that export of PfEMP1 is sl...

journal_title：Molecular microbiology

authors： Kriek N,Tilley L,Horrocks P,Pinches R,Elford BC,Ferguson DJ,Lingelbach K,Newbold CI

更新日期：2003-11-01 00:00:00

abstract：:Avermectins produced by Streptomyces avermitilis are effective anthelmintic agents. The autoregulatory signalling molecule that triggers avermectin biosynthesis is a novel butenolide-type molecule, avenolide, rather than common γ-butyrolactones (GBLs). We identified AvaR2, a pseudo GBL receptor homologue, as an import...

journal_title：Molecular microbiology

authors： Zhu J,Sun D,Liu W,Chen Z,Li J,Wen Y

更新日期：2016-11-01 00:00:00

abstract：:The DNA repeat region of fcrA76, the gene encoding a group A streptococcal Fc-binding protein, was subcloned in-frame into an Escherichia coli plasmid expression vector. The expressed protein product displayed the same Fc-binding properties as the full-length Fc-binding protein expressed from fcrA76. The affinity-puri...

journal_title：Molecular microbiology

authors： Heath DG,Boyle MD,Cleary PP

更新日期：1990-12-01 00:00:00

abstract：:The 9.5 kb virB operon is the largest of the six major operons in the Ti plasmid vir region. This operon contains eleven genes, the largest of which is virB4. This gene encodes an 84 kDa protein whose function has not been identified. Its roles in conferring virulence on Agrobacterium tumefaciens and in the T-DNA tran...

journal_title：Molecular microbiology

authors： Shirasu K,Koukolíková-Nicola Z,Hohn B,Kado CI

更新日期：1994-02-01 00:00:00

abstract：:Staphylococcus aureus strain M produces large amounts of capsular polysaccharide. It produces a non-encapsulated variant at a frequency of 0.01% at 37 degrees C. At high temperature (43 degrees C), the frequency of capsule loss was shown to be 1-38%. A 19 kb plasmid and a prophage were found to be carried by the M str...

journal_title：Molecular microbiology

authors： Lee CY

更新日期：1992-06-01 00:00:00

abstract：:Full virulence of Yersinia enterocolitica Biovar 1B requires two distinct and distantly related contact-dependent type III secretion (T3S) systems. The plasmid-encoded Ysc T3S system is essential for systemic stages of infection and the Yop effector proteins it translocates have been extensively studied. The chromosom...

journal_title：Molecular microbiology

authors： Matsumoto H,Young GM

更新日期：2006-01-01 00:00:00

abstract：:We report the cloning and determination of the nucleotide sequence of the gene encoding nucleoside diphosphate kinase (Ndk) from Pseudomonas aeruginosa. The amino acid sequence of Ndk was highly homologous with other known bacterial and eukaryotic Ndks (39.9 to 58.3% amino acid identity). We have previously reported t...

journal_title：Molecular microbiology

authors： Sundin GW,Shankar S,Chugani SA,Chopade BA,Kavanaugh-Black A,Chakrabarty AM

更新日期：1996-06-01 00:00:00

abstract：:Many types of microorganisms, including both prokaryotes and eukaryotes, have developed mechanisms to adapt to severe osmotic stress. In this study, we isolated multicopy suppressor genes for a Schizosaccharomyces pombe mutant, which exhibited the clear phenotype of being osmosensitive for growth (Osms) on agar plates...

journal_title：Molecular microbiology

authors： Ohmiya R,Yamada H,Nakashima K,Aiba H,Mizuno T

更新日期：1995-12-01 00:00:00

abstract：:Pregnancy-associated malaria (PAM) is caused by Plasmodium falciparum-infected erythrocytes (IEs) that bind to chondroitin sulphate A (CSA) in the placenta by PAM-associated clonally variant surface antigens (VSA). Pregnancy-specific VSA (VSA(PAM)), which include the PfEMP1 variant VAR2CSA, are targets of IgG-mediated...

journal_title：Molecular microbiology

authors： Barfod L,Bernasconi NL,Dahlbäck M,Jarrossay D,Andersen PH,Salanti A,Ofori MF,Turner L,Resende M,Nielsen MA,Theander TG,Sallusto F,Lanzavecchia A,Hviid L

更新日期：2007-01-01 00:00:00

abstract：:The cGvpE protein of Halobacterium salinarum PHH4 has been identified as transcriptional activator for the promoter of the c-gvpA gene encoding the major gas vesicle structural protein cGvpA. Molecular modelling of the carboxy-terminal region of cGvpE suggests that this protein resembles a basic leucine-zipper protein...

journal_title：Molecular microbiology

authors： Plösser P,Pfeifer F

更新日期：2002-07-01 00:00:00

abstract：:RecA2278-5 is a mutant RecA protein (RecAmut) bearing two amino acid substitutions, Gly-278 to Thr and Val-275 to Phe, in the alpha-helix H of the C-terminal subdomain of the protein. RecA2278-5 mutant cells are unusual in that they are thermosensitive for recombination but almost normal for DNA repair of UV damage an...

journal_title：Molecular microbiology

authors： Alexseyev AA,Baitin DM,Kuramitsu S,Ogawa T,Ogawa H,Lanzov VA

更新日期：1997-01-01 00:00:00

abstract：:The tprK gene sequence of Treponema pallidum subspecies pallidum (T. pallidum) is heterogeneous within and among isolates. Heterogeneity in the tprK open reading frame is localized in seven discrete variable (V) regions, and variability results from apparent base changes, insertions or deletions. The TprK V regions ar...

journal_title：Molecular microbiology

authors： Centurion-Lara A,LaFond RE,Hevner K,Godornes C,Molini BJ,Van Voorhis WC,Lukehart SA

更新日期：2004-06-01 00:00:00

abstract：:DNA processing reactions often involve multiple components acting in concert to achieve the desired outcome. However, it is usually difficult to know how the components communicate and cooperate to orchestrate an ordered series of events. We address this question in the context of the Tn 10 transposition reaction, in ...

journal_title：Molecular microbiology

authors： Liu D,Sewitz S,Crellin P,Chalmers R

更新日期：2006-12-01 00:00:00

abstract：:The surface-located fibrinogen-binding protein (clumping factor; ClfA) of Staphylococcus aureus has an unusual dipeptide repeat linking the ligand binding domain to the wall-anchored region. Southern blotting experiments revealed several other loci in the S. aureus Newman genome that hybridized to a probe comprising D...

journal_title：Molecular microbiology

authors： Ní Eidhin D,Perkins S,Francois P,Vaudaux P,Höök M,Foster TJ

更新日期：1998-10-01 00:00:00

abstract：:The ability of Salmonella enterica serovar Typhimurium to cause disease depends upon the co-ordinated expression of many genes located around the Salmonella chromosome. Specific pathogenicity loci, termed Salmonella pathogenicity islands, have been shown to be crucial for the invasion and survival of Salmonella within...

journal_title：Molecular microbiology

authors： Wilson RL,Libby SJ,Freet AM,Boddicker JD,Fahlen TF,Jones BD

更新日期：2001-01-01 00:00:00

abstract：:The export of large negatively charged capsular polysaccharides across the outer membrane represents a significant challenge to Gram negative bacteria. In the case of Escherichia coli group 2 capsular polysaccharides, the mechanism of export across the outer membrane was unknown, with no identified candidate outer mem...

journal_title：Molecular microbiology

authors： McNulty C,Thompson J,Barrett B,Lord L,Andersen C,Roberts IS

更新日期：2006-02-01 00:00:00