Abstract：
:The ability of Salmonella enterica serovar Typhimurium to cause disease depends upon the co-ordinated expression of many genes located around the Salmonella chromosome. Specific pathogenicity loci, termed Salmonella pathogenicity islands, have been shown to be crucial for the invasion and survival of Salmonella within host cells. Salmonella pathogenicity island 1 (SPI-1) harbours the genes required for the stimulation of Salmonella uptake across the intestinal epithelia of the infected host. Regulation of SPI-1 genes is complex, as invasion gene expression responds to a number of different signals, presumably signals similar to those found within the environment of the intestinal tract. As a result of our continued studies of SPI-1 gene regulation, we have discovered that the nucleoid-binding protein Fis plays a pivotal role in the expression of HilA and InvF, two activators of SPI-1 genes. A S. typhimurium fis mutant demonstrates a two- to threefold reduction in hilA:Tn5lacZY and a 10-fold reduction in invF:Tn5lacZY expression, as well as a 50-fold decreased ability to invade HEp-2 tissue culture cells. This decreased expression of hilA and invF resulted in an altered secreted invasion protein profile in the fis mutant. Furthermore, the virulence of a S. typhimurium fis mutant is attenuated 100-fold when administered orally, but has wild-type virulence when administered intraperitoneally. Expression of hilA:Tn5lacZY and invF:Tn5lacZY in the fis mutant could be restored by introducing a plasmid containing the S. typhimurium fis gene or a plasmid containing hilD, a gene encoding an AraC-like regulator of Salmonella invasion genes.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Wilson RL,Libby SJ,Freet AM,Boddicker JD,Fahlen TF,Jones BDdoi
10.1046/j.1365-2958.2001.02192.xsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
79-88issue
1eissn
0950-382Xissn
1365-2958pii
mmi2192journal_volume
39pub_type
杂志文章abstract：:Nitrogen metabolism in Aspergillus nidulans is regulated by AREA, a member of the GATA family of transcription factors. One mechanism that modulates AREA activity involves the rapid degradation of the areA transcript when sufficient NH4+ or Gln are available. This signalling mechanism has been shown to require a regio...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.02085.x
更新日期：2000-09-01 00:00:00
abstract：:The cytochrome d complex of Escherichia coli is a heterodimer located in the bacterial cytoplasmic membrane, where it functions as a terminal oxidase of the aerobic respiratory chain. The topology of each of the two subunits of the cytochrome d complex was analysed by the genetic method involving alkaline phosphatase ...
journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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pub_type： 杂志文章
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journal_title：Molecular microbiology
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更新日期：2000-01-01 00:00:00
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更新日期：2007-01-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1989.tb00204.x
更新日期：1989-05-01 00:00:00
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更新日期：2004-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-06-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01098.x
更新日期：1993-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-12-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1992.tb01417.x
更新日期：1992-09-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01212.x
更新日期：1993-04-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12460
更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-09-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular microbiology
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更新日期：1992-04-01 00:00:00
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journal_title：Molecular microbiology
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更新日期：2002-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03503.x
更新日期：2003-06-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：1997-07-01 00:00:00
