Abstract：
:Nitrogen metabolism in Aspergillus nidulans is regulated by AREA, a member of the GATA family of transcription factors. One mechanism that modulates AREA activity involves the rapid degradation of the areA transcript when sufficient NH4+ or Gln are available. This signalling mechanism has been shown to require a region of 218 nucleotides within the 3' untranslated region of areA mRNA. We demonstrate that this region functions independently in a heterologous transcript and acts to accelerate degradation of the poly(A) tail, which in turn leads to rapid transcript degradation in response to the addition of NH4+ or Gln to the growth medium. areA transcript degradation is inhibited by cycloheximide, but this is not a general consequence of translational inhibition. We believe that this is the first reported example in which specific physiological signals, acting through a defined sequence within a transcript, have been shown to promote accelerated poly(A) degradation, which in turn triggers transcript degradation.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Morozov IY,Martinez MG,Jones MG,Caddick MXdoi
10.1046/j.1365-2958.2000.02085.xsubject
Has Abstractpub_date
2000-09-01 00:00:00pages
1248-57issue
5eissn
0950-382Xissn
1365-2958pii
mmi2085journal_volume
37pub_type
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