Abstract:
:The nucleotide sequence of the traT gene present in the virulence-associated plasmid of Salmonella typhimurium was determined. The predicted TraT protein encoded by this gene was found to consist of 243 amino acids and to resemble the known TraT proteins of the plasmids of the F incompatibility group. Thus it contains a signal sequence of 20 amino acids, an amino-terminal lipid attachment site, and two strongly hydrophobic regions close to each other in the mature protein. A mutation leading to increased permeability of the outer membrane to hydrophobic agents, previously localized to the traT gene, was shown to change a glycine residue to arginine within one of these hydrophobic regions. The same principle was found to apply to TraT of R6-5: the introduction, by site-directed mutagenesis, of either positively or negatively charged amino acids or the helix-disrupting proline in the corresponding hydrophobic region led to increased hydrophobic permeability of the outer membrane.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Sukupolvi S,Vuorio R,Qi SY,O'Connor D,Rhen Mdoi
10.1111/j.1365-2958.1990.tb02014.xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
49-57issue
1eissn
0950-382Xissn
1365-2958journal_volume
4pub_type
杂志文章abstract::Phycobilisomes are the major constituents of the light-harvesting apparatus in both cyanobacteria and red algae and consist of a central core with radiating rods. From a genomic library of the cyanobacterium Calothrix 7601, a DNA fragment encoding allophycocyanin B, one of the two terminal energy acceptors of the core...
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