Phosphorylation of the RitR DNA-binding domain by a Ser-Thr phosphokinase: implications for global gene regulation in the streptococci.

abstract：:Bacterial chemoreceptors cluster into exquisitively sensitive, tunable, highly ordered, polar arrays. While these arrays serve as paradigms of cell signalling in general, it remains unclear what conformational changes transduce signals from the periplasmic tips, where attractants and repellents bind, to the cytoplasmi...

journal_title：Molecular microbiology

authors： Briegel A,Beeby M,Thanbichler M,Jensen GJ

更新日期：2011-11-01 00:00:00

abstract：:Folate metabolism in malaria parasites is a long-standing, clinical target for chemotherapy and prophylaxis. However, despite determination of the complete genome sequence of the lethal species Plasmodium falciparum, the pathway of de novo folate biosynthesis remains incomplete, as no candidate gene for dihydroneopter...

journal_title：Molecular microbiology

authors： Dittrich S,Mitchell SL,Blagborough AM,Wang Q,Wang P,Sims PF,Hyde JE

更新日期：2008-02-01 00:00:00

abstract：:We cloned the CaYFH1 gene that encodes the yeast frataxin homologue in Candida albicans. CaYFH1 was expressed in Deltayfh1 Saccharomyces cerevisiae cells, where it compensated for all the phenotypes tested except for the lack of cytochromes. Double DeltaCayfh1/DeltaCayfh1 mutant had severe defective growth, accumulate...

journal_title：Molecular microbiology

authors： Santos R,Buisson N,Knight SA,Dancis A,Camadro JM,Lesuisse E

更新日期：2004-10-01 00:00:00

abstract：:The spoIIIG gene encodes a sigma factor that determines prespore-specific gene expression during sporulation in Bacillus subtilis. Correct spatial and temporal expression of the spoIIIG gene depends on a number of other sporulation (spo) genes, but only one of these genes, spoIIIE, has a specific effect on spoIIIG exp...

journal_title：Molecular microbiology

authors： Foulger D,Errington J

更新日期：1989-09-01 00:00:00

abstract：:Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...

journal_title：Molecular microbiology

authors： Cho Y,Kim KH,La Rota M,Scott D,Santopietro G,Callihan M,Mitchell TK,Lawrence CB

更新日期：2009-06-01 00:00:00

abstract：:Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. It is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated b...

journal_title：Molecular microbiology

authors： Kessler RL,Contreras VT,Marliére NP,Aparecida Guarneri A,Villamizar Silva LH,Mazzarotto GACA,Batista M,Soccol VT,Krieger MA,Probst CM

更新日期：2017-06-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:Populations of surface-attached microorganisms comprising either single or multiple species are commonly referred to as biofilms. Using a simple assay for the initiation of biofilm formation (e.g. attachment to an abiotic surface) by Pseudomonas fluorescens strain WCS365, we have shown that: (i) P. fluorescens can for...

journal_title：Molecular microbiology

authors： O'Toole GA,Kolter R

更新日期：1998-05-01 00:00:00

abstract：:The transmembrane protein ToxR plays a key role in the virulence expression system of Vibrio cholerae. The activity of ToxR is dependent on its periplasmic sensor domain (ToxRp) and on the inner membrane protein ToxS. Herein, we present the Nuclear Magnetic Resonance NMR solution structure of the sensory ToxRp contain...

journal_title：Molecular microbiology

authors： Gubensäk N,Wagner GE,Schrank E,Falsone FS,Berger TMI,Pavkov-Keller T,Reidl J,Zangger K

更新日期：2020-12-23 00:00:00

abstract：:The porins of Gram-negative bacteria are responsible for the 'molecular sieve' properties of the outer membrane. They form large water-filled channels which allow the diffusion of hydrophilic molecules into the periplasmic space. Owing to the strong hydrophilicity of their amino acid sequence and the nature of their s...

journal_title：Molecular microbiology

authors： Jeanteur D,Lakey JH,Pattus F

更新日期：1991-09-01 00:00:00

abstract：:The bvg locus contains two genes, bvgA and bvgS, which control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli. We determined the nucleotide sequence of the bvg loci of Bor...

journal_title：Molecular microbiology

authors： Aricò B,Scarlato V,Monack DM,Falkow S,Rappuoli R

更新日期：1991-10-01 00:00:00

abstract：:Chlamydia is predicted to encode two alternative sigma factors that could provide a mechanism for the regulation of gene expression via alternative forms of RNA polymerase. We have demonstrated that sigma 28, one of these alternative sigma factors, is transcriptionally active. Chlamydial sigma 28 RNA polymerase was re...

journal_title：Molecular microbiology

authors： Yu HH,Tan M

更新日期：2003-10-01 00:00:00

abstract：:The nucleotide sequence of the traT gene present in the virulence-associated plasmid of Salmonella typhimurium was determined. The predicted TraT protein encoded by this gene was found to consist of 243 amino acids and to resemble the known TraT proteins of the plasmids of the F incompatibility group. Thus it contains...

journal_title：Molecular microbiology

authors： Sukupolvi S,Vuorio R,Qi SY,O'Connor D,Rhen M

更新日期：1990-01-01 00:00:00

abstract：:The GATA factor AreA is a wide-domain regulator in Aspergillus nidulans with transcriptional activation and chromatin remodelling functions. AreA interacts with the nitrate-specific Zn(2)-C(6) cluster protein NirA and both proteins cooperate to synergistically activate nitrate-responsive genes. We have previously esta...

journal_title：Molecular microbiology

authors： Berger H,Pachlinger R,Morozov I,Goller S,Narendja F,Caddick M,Strauss J

更新日期：2006-01-01 00:00:00

abstract：:Biofilm formation in Bacillus subtilis requires expression of the eps and tapA-sipW-tasA operons to synthesize the extracellular matrix components, extracellular polysaccharide and TasA amyloid proteins, respectively. Expression of both operons is inhibited by the DNA-binding protein master regulator of biofilm format...

journal_title：Molecular microbiology

authors： Winkelman JT,Bree AC,Bate AR,Eichenberger P,Gourse RL,Kearns DB

更新日期：2013-06-01 00:00:00

abstract：:The genes ftsE and ftsX are organized in one operon together with ftsY. FtsY codes for the receptor of the signal recognition particle (SRP) that functions in targeting a subset of inner membrane proteins. We have found no indications for a structural relationship between FtsE/X and FtsY. Evidence is presented that Ft...

journal_title：Molecular microbiology

authors： de Leeuw E,Graham B,Phillips GJ,ten Hagen-Jongman CM,Oudega B,Luirink J

更新日期：1999-02-01 00:00:00

abstract：:The fluidity and phase state of bacterial lipid bilayers commonly change in response to ambient environmental conditions to maintain the critical functions of the envelope as a semipermeable and selective boundary. A special, and intricate, set of alterations in membrane lipid metabolism is elicited by conditions caus...

journal_title：Molecular microbiology

authors： DiRusso CC,Nyström T

更新日期：1998-01-01 00:00:00

abstract：:Aflatoxins are polyaromatic mycotoxins that contaminate a range of food crops as a result of fungal growth and contribute to serious health problems in the developing world because of their toxicity and mutagenicity. Although relatively resistant to biotic degradation, aflatoxins can be metabolized by certain species ...

journal_title：Molecular microbiology

authors： Taylor MC,Jackson CJ,Tattersall DB,French N,Peat TS,Newman J,Briggs LJ,Lapalikar GV,Campbell PM,Scott C,Russell RJ,Oakeshott JG

更新日期：2010-11-01 00:00:00

abstract：:Trypanothione reductase (TR), a flavoprotein oxidoreductase central to the unique thiol-redox system that operates in trypanosomatid protozoa, has been proposed as a potential target for the chemotherapy of trypanosomatid infections. In this study, targeted gene replacement was used to obtain evidence that TR is an es...

journal_title：Molecular microbiology

authors： Tovar J,Wilkinson S,Mottram JC,Fairlamb AH

更新日期：1998-07-01 00:00:00

abstract：:Members of the classes Bacilli and Clostridia are able to form endospores, with clostridia representing the ancestral phylogenetic line. In contrast to Bacillus subtilis, the process of sporulation initiation at the molecular level is less well understood in Clostridium acetobutylicum, the model organism of the clostr...

journal_title：Molecular microbiology

authors： Dürre P

更新日期：2011-05-01 00:00:00

abstract：:Like many other bacteria, Burkholderia sp. take up iron in its ferric form via siderophore-dependent transporters. We observed that mutant strains of B. cenocepacia H111 unable to synthesize siderophores did not exhibit any growth defect under iron limited conditions. This finding suggested that this opportunistic pat...

journal_title：Molecular microbiology

authors： Mathew A,Eberl L,Carlier AL

更新日期：2014-02-01 00:00:00

abstract：:We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...

journal_title：Molecular microbiology

authors： Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

更新日期：2004-01-01 00:00:00

abstract：:Bacteria attach to their appropriate environmental niche by using adhesins. To maximize their contact with the environment, adhesins are often present on the ends of long hairlike structures called pili. Recently, attention has focused on pili of Gram-positive bacteria because they may be vaccine candidates in importa...

journal_title：Molecular microbiology

authors： Scott JR,Zähner D

更新日期：2006-10-01 00:00:00

abstract：:The K1 killer toxin of Saccharomyces cerevisiae consists of 103- and 83-residue alpha and beta components whose derivation, from a 316-residue precursor preprotoxin, requires processing at the alpha N-terminus (after ProArg-44), the alpha C-terminus (after ArgArg-149) and at the beta N-terminus (after LysArg-233). The...

journal_title：Molecular microbiology

authors： Zhu YS,Zhang XY,Cartwright CP,Tipper DJ

更新日期：1992-02-01 00:00:00

abstract：:The Escherichia coli DNA glycosylase Mug excises 3,N(4)-ethenocytosines (epsilon C) and uracils from DNA, but its biological function is obscure. This is because epsilon C is not found in E. coli DNA, and uracil-DNA glycosylase (Ung), a distinct enzyme, is much more efficient at removing uracils from DNA than Mug. We ...

journal_title：Molecular microbiology

authors： Mokkapati SK,Fernández de Henestrosa AR,Bhagwat AS

更新日期：2001-09-01 00:00:00

abstract：:polY, a transcriptional regulatory gene in the polyoxin biosynthetic cluster of Streptomyces cacaoi, was analysed, and its deduced product (PolY) showed amino acid sequence homology to AfsR from Streptomyces coelicolor A3(2). PolY contains an OmpR-like DNA binding domain at its N-terminal and an ATPase domain in the m...

journal_title：Molecular microbiology

authors： Li R,Liu G,Xie Z,He X,Chen W,Deng Z,Tan H

更新日期：2010-01-01 00:00:00

abstract：:The best studied role of ubiquitination is to mark proteins for destruction by the proteasome but, in addition, it has recently been shown to promote macromolecular assembly and function, and alter protein function, thus playing a regulatory role distinct from protein degradation. Deubiquinating enzymes, the ubiquitin...

journal_title：Molecular microbiology

authors： Lockington RA,Kelly JM

更新日期：2001-06-01 00:00:00

abstract：:To determine if the food-grade bacterium Lactococcus lactis holds promise as a vaccine antigen delivery vector we have investigated whether this bacterium can be made to produce high levels of a heterologous protein antigen. A regulated expression system has been developed which may be generally suitable for the expre...

journal_title：Molecular microbiology

authors： Wells JM,Wilson PW,Norton PM,Gasson MJ,Le Page RW

更新日期：1993-06-01 00:00:00

abstract：:Alternative oxidase (AOX) is a respiratory oxidase found in certain eukaryotes and bacteria; however, its role in bacterial physiology is unclear. Exploiting the genetic tractability of the bacterium Vibrio fischeri, we explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assa...

journal_title：Molecular microbiology

authors： Dunn AK,Karr EA,Wang Y,Batton AR,Ruby EG,Stabb EV

更新日期：2010-07-01 00:00:00

abstract：:While examining the molecular basis for the lack of actin-based motility for the non-pathogenic spotted fever group (SFG) R. peacockii, we identified a novel insertion sequence (IS) element, ISRpe1, disrupting the coding sequence of rickA, demonstrated to induce actin-tail polymerization for the SFG rickettsiae. This ...

journal_title：Molecular microbiology

authors： Simser JA,Rahman MS,Dreher-Lesnick SM,Azad AF

更新日期：2005-10-01 00:00:00