Abstract：
:Natural transformation is a potent driver for genetic diversification in bacterial populations. It involves exogenous DNA binding, uptake, transport and internalization into the cytoplasm, where DNA can be processed and integrated into the host chromosome. Direct visualisation of transforming DNA (tDNA) has been limited to its binding to the surface or, in the case of Gram-negative species, to its entrance into the periplasm. We present here for the first time the direct visualisation of tDNA entering the bacterial cytoplasm. We used as a model the Gram-negative pathogen Helicobacter pylori, characterised by a large intraspecies variability that results from high mutation rates and efficient horizontal gene transfer. Using fluorescently labelled DNA, we followed for up to 3 h the fate of tDNA foci formed in the periplasm and eventually internalised into the cytoplasm. By tracking at the single cell level the expression of a fluorescent protein coded by the tDNA, we show that up to 50% of the cells express the transforming phenotype. The overall transformation process in H. pylori, from tDNA uptake to expression of the recombinant gene, can take place in less than 1 h, without requiring a growth arrest, and prior to the replication of the chromosome.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Corbinais C,Mathieu A,Kortulewski T,Radicella JP,Marsin Sdoi
10.1111/mmi.13440subject
Has Abstractpub_date
2016-09-01 00:00:00pages
1039-53issue
6eissn
0950-382Xissn
1365-2958journal_volume
101pub_type
杂志文章abstract：:Small regulatory RNAs (sRNAs) are well known to command bacterial protein synthesis by modulating the translation and decay of target mRNAs. Most sRNAs are specifically regulated by a cognate transcription factor under certain growth or stress conditions. Investigations of the conserved Hfq-dependent MicM sRNA in Esch...
journal_title：Molecular microbiology
pub_type： 评论,杂志文章
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journal_title：Molecular microbiology
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doi：10.1046/j.1365-2958.1996.00112.x
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pub_type： 评论,杂志文章
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pub_type： 杂志文章
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journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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