Abstract:
:When they infect Escherichia coli cells, the filamentous phages IF1 and fd first interact with a pilus and then target TolA as their common receptor. They use the domains N2 and N1 of their gene-3-proteins (G3P) for these interactions but differ in the mechanism of infection. In G3P of phage IF1, N1 and N2 are independent modules that are permanently binding-active. G3P of phage fd is usually in a closed state in which N1 and N2 are tightly associated. The TolA binding site is thus inaccessible and the phage incompetent for infection. Partial unfolding and prolyl isomerization must occur to abolish the domain interactions and expose the TolA binding site. This complex mechanism of phage fd could be changed to the simple infection mechanism of phage IF1 by reprogramming its G3P following physicochemical rules of protein stability. The redesigned phage fd was robust and as infectious as wild-type phage fd.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Lorenz SH,Schmid FXdoi
10.1111/j.1365-2958.2011.07617.xsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
827-34issue
3eissn
0950-382Xissn
1365-2958journal_volume
80pub_type
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