Abstract:
:RbpA is an RNA polymerase-binding protein that occurs in the actinomycete family of bacteria and is regulated by the disulphide stress-response sigma factor, sigma(R), in Streptomyces coelicolor. Here we demonstrate that rbpA null mutants exhibit a slow-growth phenotype and are particularly sensitive to the transcription inhibitor rifampicin. Strikingly, transcription mapping experiments revealed that rbpA expression is induced upon exposure of S. coelicolor to rifampicin and that this, in part, involves an increase in the activity of sigma(R). In contrast, the ribosomal RNA operon promoter rrnDp3, which is recognized by the vegetative sigma factor sigma(HrdB), was strongly inhibited by rifampicin. Reconstitution of RNAP from an rbpA null mutant with purified RbpA revealed that RbpA stimulates transcription from rrnDp3, even in the presence of rifampicin. The data presented suggest that RbpA confers basal levels of rifampicin resistance and is a novel regulator of rRNA synthesis in S. coelicolor.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Newell KV,Thomas DP,Brekasis D,Paget MSdoi
10.1111/j.1365-2958.2006.05116.xsubject
Has Abstractpub_date
2006-05-01 00:00:00pages
687-96issue
3eissn
0950-382Xissn
1365-2958pii
MMI5116journal_volume
60pub_type
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