Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology.


:Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient molecules, perhaps even predating the existence of the translation machinery. Albeit old, these molecules are tremendously conserved, a characteristic that is well illustrated by the fact that some bacterial tRNAs are efficient and specific substrates of eukaryotic aminoacyl-tRNA synthetases and ribosomes. Considering their ancient origin and high structural conservation, it is not surprising that tRNAs have been hijacked during evolution for functions outside of translation. These roles beyond translation include synthetic, regulatory and information functions within the cell. Here we provide an overview of the non-canonical roles of tRNAs and their mimics in bacteria, and discuss some of the common themes that arise when comparing these different functions.


Mol Microbiol


Molecular microbiology


Katz A,Elgamal S,Rajkovic A,Ibba M




Has Abstract


2016-08-01 00:00:00












  • In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.

    abstract::DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular dom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

    更新日期:2005-01-01 00:00:00

  • An acetylase with relaxed specificity catalyses protein N-terminal acetylation in Sulfolobus solfataricus.

    abstract::N-terminal protein acetylation is common in eukaryotes and halophilic archaea, but very rare in bacteria. We demonstrate that some of the most abundant proteins present in the crenarchaeote Sulfolobus solfataricus, including subunits of the thermosome, proteosome and ribosome, are acetylated at the N-terminus. Modific...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Mackay DT,Botting CH,Taylor GL,White MF

    更新日期:2007-06-01 00:00:00

  • A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor.

    abstract::Deletion of gene Rv3676 in Mycobacterium tuberculosis coding for a transcription factor belonging to the cAMP receptor protein (CRP) family caused growth defects in laboratory medium, in bone marrow-derived macrophages and in a mouse model of tuberculosis. Transcript profiling of M. tuberculosis grown in vitro identif...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

    更新日期:2005-06-01 00:00:00

  • Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly.

    abstract::Coli surface antigen 6 (CS6) is a widely expressed enterotoxigenic Escherichia coli (ETEC) colonization factor that mediates bacterial attachment to the small intestinal epithelium. CS6 is a polymer of two protein subunits CssA and CssB, which are secreted and assembled on the cell surface via the CssC/CssD chaperone ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Roy SP,Rahman MM,Yu XD,Tuittila M,Knight SD,Zavialov AV

    更新日期:2012-12-01 00:00:00

  • Mutational and physical analysis of F plasmid traY protein binding to oriT.

    abstract::F plasmid traY protein binding to wild-type or deleted regions containing the TraY-binding site, sbyA, was studied in vitro. The principal DNA-protein complex was formed with DNA segments including the sbyA site defined by footprinting and (with lesser affinity) with truncated segments that retained the leftward two-t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Luo Y,Gao Q,Deonier RC

    更新日期:1994-02-01 00:00:00

  • Cloning of genes affecting capsule expression in Staphylococcus aureus strain M.

    abstract::Staphylococcus aureus strain M produces large amounts of capsular polysaccharide. It produces a non-encapsulated variant at a frequency of 0.01% at 37 degrees C. At high temperature (43 degrees C), the frequency of capsule loss was shown to be 1-38%. A 19 kb plasmid and a prophage were found to be carried by the M str...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lee CY

    更新日期:1992-06-01 00:00:00

  • Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)-D-mannose.

    abstract::Pseudomonas aeruginosa can express two distinct forms of lipopolysaccharide (LPS), called A-band and B-band. As an attempt to understand the molecular biology of the synthesis and regulation of these LPS antigens, a recombinant plasmid, pFV3, containing genes for A-band expression was isolated previously. In the prese...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lightfoot J,Lam JS

    更新日期:1993-05-01 00:00:00

  • Targeted deletion of Plasmodium knowlesi Duffy binding protein confirms its role in junction formation during invasion.

    abstract::Red cell invasion by Plasmodium merozoites involves multiple steps such as attachment, apical reorientation, junction formation and entry into a parasitophorous vacuole. These steps are mediated by specific molecular interactions. P. vivax and the simian parasite P. knowlesi require interaction with the Duffy blood gr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Singh AP,Ozwara H,Kocken CH,Puri SK,Thomas AW,Chitnis CE

    更新日期:2005-03-01 00:00:00

  • Induction of the heat shock regulon in response to increased mistranslation requires oxidative modification of the malformed proteins.

    abstract::The Escherichia coli rpsD12 allele, which reduces translational fidelity and elevates expression of heat shock protein (Hsp) genes, only enhanced Hsp gene expression in the presence of oxygen. Similarly, the rpsL141 allele, which reduces mistranslation and Hsp gene expression, failed to affect the Hsp regulon in cells...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Fredriksson A,Ballesteros M,Dukan S,Nyström T

    更新日期:2006-01-01 00:00:00

  • Salmonella enterica serotype Typhimurium ShdA is an outer membrane fibronectin-binding protein that is expressed in the intestine.

    abstract::The shdA gene is the only determinant known to be required for persistence of Salmonella enterica serotype Typhimurium (S. Typhimurium) in the murine caecum and for efficient and prolonged shedding of the organism with the faeces. To study the biological activity of the ShdA protein, we examined its expression and bin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kingsley RA,Santos RL,Keestra AM,Adams LG,Bäumler AJ

    更新日期:2002-02-01 00:00:00

  • The chromosomal relBE2 toxin-antitoxin locus of Streptococcus pneumoniae: characterization and use of a bioluminescence resonance energy transfer assay to detect toxin-antitoxin interaction.

    abstract::Proteic toxin-antitoxin (TA) loci were first identified in bacterial plasmids, and they were regarded as involved in stable plasmid maintenance by a so-called 'addiction' mechanism. Later, chromosomally encoded TA loci were identified and their function ascribed to survival mechanisms when bacteria were subjected to s...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nieto C,Pellicer T,Balsa D,Christensen SK,Gerdes K,Espinosa M

    更新日期:2006-02-01 00:00:00

  • A novel RNA polymerase-binding protein controlling genes involved in spore germination in Bacillus subtilis.

    abstract::A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Traag BA,Ramirez-Peralta A,Wang Erickson AF,Setlow P,Losick R

    更新日期:2013-07-01 00:00:00

  • The conserved proline-rich motif is not essential for energy transduction by Escherichia coli TonB protein.

    abstract::TonB protein functions as an energy transducer, coupling cytoplasmic membrane electrochemical potential to the active transport of vitamin B12 and Fe(III)-siderophore complexes across the outer membrane of Escherichia coli and other Gram-negative bacteria. Accumulated evidence indicates that TonB is anchored in the cy...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Larsen RA,Wood GE,Postle K

    更新日期:1993-12-01 00:00:00

  • Characterization of secreted sphingosine-1-phosphate lyases required for virulence and intracellular survival of Burkholderia pseudomallei.

    abstract::Sphingosine-1-phosphate (S1P), a bioactive sphingolipid metabolite, plays a critical role in the orchestration of immune responses. S1P levels within the mammalian host are tightly regulated, in part through the activity of S1P lyase (S1PL) which catalyses its irreversible degradation. Herein, we describe the identifi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Custódio R,McLean CJ,Scott AE,Lowther J,Kennedy A,Clarke DJ,Campopiano DJ,Sarkar-Tyson M,Brown AR

    更新日期:2016-12-01 00:00:00

  • Variations in the surface proteins and restriction enzyme systems of Mycoplasma pulmonis in the respiratory tract of infected rats.

    abstract::Restriction and modification (R-M) systems are generally thought to protect bacteria from invasion by foreign DNA. This paper proposes the existence of an alternative role for the phase-variable R-M systems encoded by the hsd loci of Mycoplasma pulmonis. Populations of M. pulmonis cells that arose during growth in dif...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gumulak-Smith J,Teachman A,Tu AH,Simecka JW,Lindsey JR,Dybvig K

    更新日期:2001-05-01 00:00:00

  • Oscillin, an extracellular, Ca2+-binding glycoprotein essential for the gliding motility of cyanobacteria.

    abstract::Electron microscopic studies have demonstrated that various gliding filamentous cyanobacteria have trichome surfaces with a common structural organization. They contain an S-layer attached to the outer membrane and an array of parallel fibrils on top of the S-layer. In all species studied, the helical arrangement of t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hoiczyk E,Baumeister W

    更新日期:1997-11-01 00:00:00

  • Genetic identification of exported proteins in Streptococcus pneumoniae.

    abstract::A strategy was developed to mutate and genetically identify exported proteins in Streptococcus pneumoniae. Vectors were created and used to screen pneumococcal DNA in Escherichia coli and S. pneumoniae for translational gene fusions to alkaline phosphatase (PhoA). Twenty five PhoA+ pneumococcal mutants were isolated a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pearce BJ,Yin YB,Masure HR

    更新日期:1993-09-01 00:00:00

  • Nucleotide sequence and mutational analysis indicate that two Helicobacter pylori genes encode a P-type ATPase and a cation-binding protein associated with copper transport.

    abstract::A 2.7 kb fragment of Helicobacter pylori UA802 chromosomal DNA was cloned and sequenced. Three open reading frames (designated ORF1, ORF2 and ORF3, respectively) were predicted from the DNA sequence, of which ORF1 and ORF2 appeared to be located within the same operon. The deduced 611-amino-acid sequence of ORF1, a P-...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ge Z,Hiratsuka K,Taylor DE

    更新日期:1995-01-01 00:00:00

  • Towards a systematic understanding of structure-function relationship of dimethylsulfoniopropionate-catabolizing enzymes.

    abstract::Each year, several million tons of dimethylsulfoniopropionate (DMSP) are produced by marine phytoplankton and bacteria as an important osmolyte to regulate their cellular osmosis. Microbial breakdown of DMSP to the volatile gas dimethylsulfide (DMS) plays an important role in global biogeochemical cycles of the sulphu...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: Chen Y,Schäfer H

    更新日期:2019-06-01 00:00:00

  • Xanthine dehydrogenase from the phototrophic purple bacterium Rhodobacter capsulatus is more similar to its eukaryotic counterparts than to prokaryotic molybdenum enzymes.

    abstract::Fourteen Rhodobacter capsulatus mutants unable to grow with xanthine as sole nitrogen source were isolated by random Tn5 mutagenesis. Five of these Tn5 insertions were mapped within two adjacent chromosomal EcoRI fragments hybridizing to oligonucleotides synthesized according to conserved amino acid sequences of eukar...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Leimkühler S,Kern M,Solomon PS,McEwan AG,Schwarz G,Mendel RR,Klipp W

    更新日期:1998-02-01 00:00:00

  • Mutations in the multi-drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole-resistant Candida albicans strains.

    abstract::Overexpression of the MDR1 gene, encoding a multi-drug efflux pump of the major facilitator superfamily, is a major cause of resistance to the widely used antifungal agent fluconazole and other toxic substances in the fungal pathogen Candida albicans. We found that all tested clinical and in vitro generated C. albican...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dunkel N,Blass J,Rogers PD,Morschhäuser J

    更新日期:2008-08-01 00:00:00

  • An account of evolutionary specialization: the AbcR small RNAs in the Rhizobiales.

    abstract::The AbcR small RNAs (sRNAs) are a fascinating example of two highly conserved sRNAs that differ tremendously at the functional level among organisms. From their transcriptional activation to their regulatory capabilities, the AbcR sRNAs exhibit varying characteristics in three well-studied bacteria belonging to the Rh...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Sheehan LM,Caswell CC

    更新日期:2018-01-01 00:00:00

  • Host-dependent requirement for specific DnaA boxes for plasmid RK2 replication.

    abstract::The replication origin of the broad-host-range plasmid RK2, oriV, contains four DnaA boxes, which bind the DnaA protein isolated from Escherichia coli. Using a transformation assay, mutational analysis of these boxes showed a differential requirement for replication in different Gram-negative bacteria. DnaA boxes 3 an...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Doran KS,Helinski DR,Konieczny I

    更新日期:1999-08-01 00:00:00

  • Of mothballs and old yellow enzymes.

    abstract::Polycyclic aromatic hydrocarbons (PAHs) are persistent and toxic environmental pollutants that accumulate in anoxic habitats. With the exception of naphthalene, nothing is known about the microbial degradation of PAH in these environments. The challenge that must be met in anaerobic PAH degradation is the destabilizat...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: Sawers RG

    更新日期:2015-01-01 00:00:00

  • Genetic ablation of a Maurer's cleft protein prevents assembly of the Plasmodium falciparum virulence complex.

    abstract::The malaria parasite Plasmodium falciparum assembles knob structures underneath the erythrocyte membrane that help present the major virulence protein, P. falciparum erythrocyte membrane protein-1 (PfEMP1). Membranous structures called Maurer's clefts are established in the erythrocyte cytoplasm and function as sortin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dixon MW,Kenny S,McMillan PJ,Hanssen E,Trenholme KR,Gardiner DL,Tilley L

    更新日期:2011-08-01 00:00:00

  • AUG-proximal nucleotides regulate protein synthesis in Leishmania tropica.

    abstract::Gene expression in the Leishmania is controlled post-transcriptionally, and is likely to be impacted by both 5' and 3' untranslated regions (UTRs). We have investigated the effects of trinucleotides in the AUG-proximal region (APR) (i.e. positions -3 to -1 upstream of an AUG) on two reporter genes in the context of an...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Stanton JD,Mensa-Wilmot K

    更新日期:2006-08-01 00:00:00

  • Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli.

    abstract::Laboratory-based evolution and whole-genome sequencing can link genotype and phenotype. We used evolution of acid resistance in exponential phase Escherichia coli to study resistance to a lethal stress. Iterative selection at pH 2.5 generated five populations that were resistant to low pH in early exponential phase. G...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Johnson MD,Bell J,Clarke K,Chandler R,Pathak P,Xia Y,Marshall RL,Weinstock GM,Loman NJ,Winn PJ,Lund PA

    更新日期:2014-09-01 00:00:00

  • Multiple novel promoters from the early region in the Streptomyces temperate phage phi C31 are activated during lytic development.

    abstract::Evidence is presented that transcription of most of the early genes in the Streptomyces coelicolor A3(2) phage phi C31 is from a series of unusual promoters that depend on a function expressed early in the phi C31 lytic cycle. Primer extension analysis on the 5' ends of three early mRNAs, from samples prepared 10 min ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ingham CJ,Crombie HJ,Bruton CJ,Chater KF,Hartley NM,Murphy GJ,Smith MC

    更新日期:1993-09-01 00:00:00

  • Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence.

    abstract::Listeria monocytogenes is a Gram-positive intracellular parasite and the causative organism of human listeriosis. In this article we demonstrate that L. monocytogenes encodes a functional member of the CodY family of global regulatory proteins that is responsive to both GTP and branched chain amino acids. By transcrip...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bennett HJ,Pearce DM,Glenn S,Taylor CM,Kuhn M,Sonenshein AL,Andrew PW,Roberts IS

    更新日期:2007-03-01 00:00:00

  • Identification of exopolysaccharide-deficient mutants of Mycoplasma pulmonis.

    abstract::The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Daubenspeck JM,Bolland JR,Luo W,Simmons WL,Dybvig K

    更新日期:2009-06-01 00:00:00