Abstract：
:A histidine kinase, Hik33, appears to sense decreases in temperature and to regulate the expression of certain cold-inducible genes in the cyanobacterium Synechocystis sp. PCC6803. To examine the role of Hik33 in the regulation of gene expression, we analysed a DeltaHik33 mutant using the DNA microarray technique. In wild-type cells, genes that were strongly induced at low temperature encoded proteins that were predominantly subunits of the transcriptional and translational machinery. Most cold-repressible genes encoded components of the photosynthetic machinery. Mutation of the hik33 gene suppressed the expression of some of these cold-regulated genes, which could be divided into three groups according to the effect of the mutation of hik33. In the first group, regulation of gene expression by low temperature was totally abolished; in the second group, the extent of such regulation was reduced by half; and, in the third group, such regulation was totally unaffected. These results suggest that expression of the genes in the first group is regulated solely by Hik33, expression of genes in the third group is regulated by an as yet unidentified cold sensor, and expression of genes in the second group is regulated by both these cold sensors.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Suzuki I,Kanesaki Y,Mikami K,Kanehisa M,Murata Ndoi
10.1046/j.1365-2958.2001.02379.xsubject
Has Abstractpub_date
2001-04-01 00:00:00pages
235-44issue
1eissn
0950-382Xissn
1365-2958pii
mmi2379journal_volume
40pub_type
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