Cold-regulated genes under control of the cold sensor Hik33 in Synechocystis.


:A histidine kinase, Hik33, appears to sense decreases in temperature and to regulate the expression of certain cold-inducible genes in the cyanobacterium Synechocystis sp. PCC6803. To examine the role of Hik33 in the regulation of gene expression, we analysed a DeltaHik33 mutant using the DNA microarray technique. In wild-type cells, genes that were strongly induced at low temperature encoded proteins that were predominantly subunits of the transcriptional and translational machinery. Most cold-repressible genes encoded components of the photosynthetic machinery. Mutation of the hik33 gene suppressed the expression of some of these cold-regulated genes, which could be divided into three groups according to the effect of the mutation of hik33. In the first group, regulation of gene expression by low temperature was totally abolished; in the second group, the extent of such regulation was reduced by half; and, in the third group, such regulation was totally unaffected. These results suggest that expression of the genes in the first group is regulated solely by Hik33, expression of genes in the third group is regulated by an as yet unidentified cold sensor, and expression of genes in the second group is regulated by both these cold sensors.


Mol Microbiol


Molecular microbiology


Suzuki I,Kanesaki Y,Mikami K,Kanehisa M,Murata N




Has Abstract


2001-04-01 00:00:00














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    abstract::Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity. Both Fis and H-NS selectively repress the dps pro...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Grainger DC,Goldberg MD,Lee DJ,Busby SJ

    更新日期:2008-06-01 00:00:00

  • Biochemical characterization of a Neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases.

    abstract::The extracellular polysaccharide capsule is an essential virulence factor of Neisseria meningitidis, a leading cause of severe bacterial meningitis and sepsis. Serogroup B strains, the primary disease causing isolates in Europe and America, are encapsulated in alpha-2,8 polysialic acid (polySia). The capsular polymer ...

    journal_title:Molecular microbiology

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    authors: Freiberger F,Claus H,Günzel A,Oltmann-Norden I,Vionnet J,Mühlenhoff M,Vogel U,Vann WF,Gerardy-Schahn R,Stummeyer K

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  • Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR.

    abstract::The Escherichia coli marRAB operon specifies two regulatory proteins, MarR (which represses) and MarA (which activates expression of the operon). The latter controls expression of multiple other chromosomal genes implicated in cell physiology, multiple drug resistance and virulence. Using randomly cloned E. coli DNA f...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,收录出版


    authors: Domain F,Bina XR,Levy SB

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  • Studies on the structure and function of the N-terminal domain of the pneumococcal murein hydrolases.

    abstract::The structures of the choline-dependent pneumococcal murein hydrolases, LYTA amidase and CPL1 lysozyme, and the choline-independent CPL7 lysozyme were analysed by controlled proteolytic digestions. The trypsin cleavage of the CPL1 and CPL7 lysozymes produced two resistant polypeptides, F1 and F7 respectively, correspo...

    journal_title:Molecular microbiology

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    authors: Sanz JM,Díaz E,García JL

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  • A novel DNA modification by sulphur.

    abstract::Streptomyces lividans has a novel DNA modification, which sensitises its DNA to degradation during electrophoresis (the Dnd phenotype). The entire gene cluster (dnd) involved in this modification was localized on an 8 kb DNA fragment and was expressed in a S. lividans deletion mutant (dnd) and in several heterologous ...

    journal_title:Molecular microbiology

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    authors: Zhou X,He X,Liang J,Li A,Xu T,Kieser T,Helmann JD,Deng Z

    更新日期:2005-09-01 00:00:00

  • Following transforming DNA in Helicobacter pylori from uptake to expression.

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    authors: Corbinais C,Mathieu A,Kortulewski T,Radicella JP,Marsin S

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    journal_title:Molecular microbiology

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    authors: Słomińska M,Konopa G,Ostrowska J,Kedzierska B,Wegrzyn G,Wegrzyn A

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    authors: El Alami M,Feller A,Piérard A,Dubois E

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  • Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse.

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    pub_type: 杂志文章


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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lee SC,Li A,Calo S,Inoue M,Tonthat NK,Bain JM,Louw J,Shinohara ML,Erwig LP,Schumacher MA,Ko DC,Heitman J

    更新日期:2015-09-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Heilmann C,Schweitzer O,Gerke C,Vanittanakom N,Mack D,Götz F

    更新日期:1996-06-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: McKellar JL,Minnell JJ,Gerth ML

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    pub_type: 杂志文章,评审


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    更新日期:2020-04-01 00:00:00