Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence.

abstract：:Sphingosine-1-phosphate (S1P), a bioactive sphingolipid metabolite, plays a critical role in the orchestration of immune responses. S1P levels within the mammalian host are tightly regulated, in part through the activity of S1P lyase (S1PL) which catalyses its irreversible degradation. Herein, we describe the identifi...

journal_title：Molecular microbiology

authors： Custódio R,McLean CJ,Scott AE,Lowther J,Kennedy A,Clarke DJ,Campopiano DJ,Sarkar-Tyson M,Brown AR

更新日期：2016-12-01 00:00:00

abstract：:5'-nucleotidases (5'-NTs) are enzymes that catalyze the hydrolysis of nucleoside monophosphates to produce nucleosides and phosphate. Since the identification of adenosine synthase A (AdsA) in Staphylococcus aureus in 2009, several other 5'-NTs have been discovered in Gram-positive cocci, mainly in streptococci. Despi...

journal_title：Molecular microbiology

authors： Soh KY,Loh JMS,Proft T

更新日期：2020-04-01 00:00:00

abstract：:A hallmark of the biofilm architecture is the presence of microcolonies. However, little is known about the underlying mechanisms governing microcolony formation. In the pathogen Pseudomonas aeruginosa, microcolony formation is dependent on the two-component regulator MifR, with mifR mutant biofilms exhibiting an over...

journal_title：Molecular microbiology

authors： Petrova OE,Schurr JR,Schurr MJ,Sauer K

更新日期：2012-11-01 00:00:00

abstract：:Many bacterial gene regulatory circuits are controlled by temperature. Temperature-mediated regulation occurs at the level of transcription and translation. Supercoiling, changes in mRNA conformation and protein conformation are all implicated in thermosensing. Bacterial virulence functions are often temperature regul...

journal_title：Molecular microbiology

authors： Hurme R,Rhen M

更新日期：1998-10-01 00:00:00

abstract：:Bacterial biofilms contain subpopulations of cells that are dormant and highly tolerant to antibiotics. While dormant, the bacteria must maintain the integrity of macromolecules required for resuscitation. Previously, we showed that hibernation promoting factor (HPF) is essential for protecting Pseudomonas aeruginosa ...

journal_title：Molecular microbiology

authors： Akiyama T,Williamson KS,Franklin MJ

更新日期：2018-10-01 00:00:00

abstract：:Transcription of lysine genes in Saccharomyces cerevisiae is dependent on Lys14p and on alpha-aminoadipate semialdehyde (alphaAASA), an intermediate of the pathway. The two-thirds C-terminal end of Lys14p is sufficient to ensure the activation function of the protein and its modulation by alphaAASA. Here, we show that...

journal_title：Molecular microbiology

authors： El Alami M,Feller A,Piérard A,Dubois E

更新日期：2002-03-01 00:00:00

abstract：:Initiation of Escherichia coli chromosome replication is controlled by the DnaA initiator protein. Both rifampicin-mediated inhibition of transcription and ppGpp-induced changes in global transcription stops replication at the level of initiation. Here, we show that continued DnaA protein synthesis allows for replicat...

journal_title：Molecular microbiology

authors： Riber L,Løbner-Olesen A

更新日期：2020-12-01 00:00:00

abstract：:In contrast to many antimicrobial peptides, members of the proline-rich group of antimicrobial peptides inactivate Gram-negative bacteria by a non-lytic mechanism. Several lines of evidence indicate that they are internalized into bacteria and their activity mediated by interaction with unknown cellular components. Wi...

journal_title：Molecular microbiology

authors： Mattiuzzo M,Bandiera A,Gennaro R,Benincasa M,Pacor S,Antcheva N,Scocchi M

更新日期：2007-10-01 00:00:00

abstract：:Fragments of the gene encoding the major outer membrane porin protein (MOMP) of Chlamydia trachomatis serovar L1 were ligated into the pUC plasmid vectors to give a series of overlapping recombinants expressing MOMP from the lac promoter. Induction of this promoter with IPTG leads to high-level expression of the recom...

journal_title：Molecular microbiology

authors： Pickett MA,Ward ME,Clarke IN

更新日期：1988-09-01 00:00:00

abstract：:Reverse transcriptase, discovered in 1970 in retroviruses, has until recently been found only in eukaryotic organisms. Recently it was shown to occur in two groups of bacteria: myxobacteria and Escherichia coli. The gene for reverse transcriptase is part of a chromosomal genetic element that codes for the production o...

journal_title：Molecular microbiology

authors： Lim D,Maas WK

更新日期：1989-08-01 00:00:00

abstract：:In enteric bacteria, proteins of the Hha/YmoA family play a role in the regulation of gene expression in response to environmental factors. Interaction of both Hha and YmoA with H-NS has been reported, and an Hha/H-NS complex has been shown to modulate expression in Escherichia coli of the haemolysin operon of plasmid...

journal_title：Molecular microbiology

authors： Paytubi S,Madrid C,Forns N,Nieto JM,Balsalobre C,Uhlin BE,Juárez A

更新日期：2004-10-01 00:00:00

abstract：:Lyme disease is a tick-transmitted infection caused by the spirochete Borrelia burgdorferi. Ticks deposit B. burgdorferi into the dermis of the host, where they eventually become associated with collagen fibres. We demonstrated previously that B. burgdorferi is unable to bind collagen, but can bind the collagen-associ...

journal_title：Molecular microbiology

authors： Guo BP,Brown EL,Dorward DW,Rosenberg LC,Höök M

更新日期：1998-11-01 00:00:00

abstract：:Wild strains of Bacillus subtilis are capable of forming architecturally complex communities of cells known as biofilms. Critical to biofilm formation is the eps operon, which is believed to be responsible for the biosynthesis of an exopolysaccharide that binds chains of cells together in bundles. We report that trans...

journal_title：Molecular microbiology

authors： Kearns DB,Chu F,Branda SS,Kolter R,Losick R

更新日期：2005-02-01 00:00:00

abstract：:In Escherichia coli, the SeqA protein is known as a negative regulator of chromosome replication. This protein is also suggested to have a role in chromosome organization. SeqA preferentially binds to hemi-methylated DNA and is by immunofluorescence microscopy seen as foci situated at the replication factories. Loss o...

journal_title：Molecular microbiology

authors： Klungsøyr HK,Skarstad K

更新日期：2004-10-01 00:00:00

abstract：:Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysi...

journal_title：Molecular microbiology

authors： Vimberg V,Xiong L,Bailey M,Tenson T,Mankin A

更新日期：2004-10-01 00:00:00

abstract：:In May 2004, over 100 bacteriologists from 19 different countries discussed recent progress in identification and understanding of individual signal transfer mechanisms in bacteria and in the mutual interactions between these systems to form a functional living cell. The meeting was held in San Feliu and supported by ...

journal_title：Molecular microbiology

authors： Hellingwerf KJ

更新日期：2004-10-01 00:00:00

abstract：:RpoS, an alternative primary sigma factor, has been shown to be regulated at multiple levels, including transcription, translation and protein stability. Here, we present evidence that suggests that RpoS is regulated at yet another level by the product of the crl gene. The crl gene was first thought to encode the majo...

journal_title：Molecular microbiology

authors： Pratt LA,Silhavy TJ

更新日期：1998-09-01 00:00:00

abstract：:The interactions between pathogenic bacteria and the host need to be resolved at the molecular level in order to develop novel vaccines and drugs. We have previously identified strepadhesin, a novel glycoprotein-binding activity in Streptococcus pyogenes, which is regulated by Mga, a regulator of streptococcal virulen...

journal_title：Molecular microbiology

authors： Hytönen J,Haataja S,Gerlach D,Podbielski A,Finne J

更新日期：2001-01-01 00:00:00

abstract：:Ethylenediaminetetraacetate (EDTA) is currently the most abundant organic pollutant due to its recalcitrance and extensive use. Only a few bacteria can degrade it, using EDTA monooxygenase (EmoA) to initiate the degradation. EmoA is an FMNH2 -dependent monooxygenase that requires an NADH:FMN oxidoreductase (EmoB) to p...

journal_title：Molecular microbiology

authors： Jun SY,Lewis KM,Youn B,Xun L,Kang C

更新日期：2016-06-01 00:00:00

abstract：:Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA, is controlled by at least three mechanisms: specific induction by xylan or xylose; carbon catabolite repression (CCR); and regulation by ambient pH. Deletion analysis of xlnA upstream sequences has identified two positively acting regions: one that...

journal_title：Molecular microbiology

authors： Orejas M,MacCabe AP,Pérez González JA,Kumar S,Ramón D

更新日期：1999-01-01 00:00:00

abstract：:The Bacillus subtilis genome encodes three apparent lipoyl ligase homologues: yhfJ, yqhM and ywfL, which we have renamed lplJ, lipM and lipL respectively. We show that LplJ encodes the sole lipoyl ligase of this bacterium. Physiological and biochemical characterization of a ΔlipM strain showed that LipM is absolutely ...

journal_title：Molecular microbiology

authors： Martin N,Christensen QH,Mansilla MC,Cronan JE,de Mendoza D

更新日期：2011-04-01 00:00:00

abstract：:A model for the topology of the PhoE porin has been proposed according to which the polypeptide traverses the outer membrane sixteen times mostly as amphipathic beta-sheets, thereby exposing eight loops at the cell surface. Until now, no evidence has been obtained for the surface exposure of the third loop. Recently, ...

journal_title：Molecular microbiology

authors： Struyvé M,Visser J,Adriaanse H,Benz R,Tommassen J

更新日期：1993-01-01 00:00:00

abstract：:The earliest event in bacterial cell division is the formation of a Z ring, composed of the tubulin-like FtsZ protein, at the division site at midcell. This ring then recruits several other division proteins and together they drive the formation of a division septum between two replicated chromosomes. Here we show tha...

journal_title：Molecular microbiology

authors： Peters PC,Migocki MD,Thoni C,Harry EJ

更新日期：2007-04-01 00:00:00

abstract：:Proteins with LytM (Peptidase_M23) domains are broadly distributed in bacteria and have been implicated in a variety of important processes, including cell division and cell-shape determination. Most LytM-like proteins that have been structurally and/or biochemically characterized are metallo-endopeptidases that cleav...

journal_title：Molecular microbiology

authors： Peters NT,Morlot C,Yang DC,Uehara T,Vernet T,Bernhardt TG

更新日期：2013-08-01 00:00:00

abstract：:SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged proteins by means of a genetically engineered SsrA and identified some of them. Analysis of the proteins suggested that they are tagged at their C-terminal extremit...

journal_title：Molecular microbiology

authors： Collier J,Binet E,Bouloc P

更新日期：2002-08-01 00:00:00

abstract：:We cloned the CaYFH1 gene that encodes the yeast frataxin homologue in Candida albicans. CaYFH1 was expressed in Deltayfh1 Saccharomyces cerevisiae cells, where it compensated for all the phenotypes tested except for the lack of cytochromes. Double DeltaCayfh1/DeltaCayfh1 mutant had severe defective growth, accumulate...

journal_title：Molecular microbiology

authors： Santos R,Buisson N,Knight SA,Dancis A,Camadro JM,Lesuisse E

更新日期：2004-10-01 00:00:00

abstract：:The cyclic AMP protein kinase A pathway governs numerous biological features of the fungal pathogen Candida albicans. The catalytic protein kinase A subunits, Tpk1 (orf19.4892) and Tpk2 (orf19.2277), have divergent roles, and most studies indicate a more pronounced role for Tpk2. Here we dissect two Tpk1-responsive pr...

journal_title：Molecular microbiology

authors： Fanning S,Xu W,Beaurepaire C,Suhan JP,Nantel A,Mitchell AP

更新日期：2012-10-01 00:00:00

abstract：:Pathogenic enterotoxigenic Escherichia coli (ETEC) are the major bacterial cause of diarrhea in young children in developing countries and in travelers, causing significant mortality in children. Adhesive fimbriae are a prime virulence factor for ETEC, initiating colonization of the small intestinal epithelium. Simila...

journal_title：Molecular microbiology

authors： Mortezaei N,Epler CR,Shao PP,Shirdel M,Singh B,McVeigh A,Uhlin BE,Savarino SJ,Andersson M,Bullitt E

更新日期：2015-01-01 00:00:00

abstract：:There is a DNA sequence which has unusually high affinity for the DnaA protein of Escherichia coli between the glyV and amiB-mutL operons at 94.7 min on the genetic map. Affinity of DnaA protein for DNA was measured in vivo as the activity of beta-galactosidase produced from the lacZ gene on a plasmid fused to the 5'-...

journal_title：Molecular microbiology

authors： Kitagawa R,Mitsuki H,Okazaki T,Ogawa T

更新日期：1996-03-01 00:00:00

abstract：:Maturation of c-type cytochromes in Escherichia coli is a complex process requiring eight membrane proteins encoded by the ccmABCDEFGH operon. CcmE is a mediator of haem delivery. It binds haem transiently at a conserved histidine residue and releases it for directed transfer to apocytochrome c. CcmC, an integral memb...

journal_title：Molecular microbiology

authors： Schulz H,Pellicioli EC,Thöny-Meyer L

更新日期：2000-09-01 00:00:00