High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1.

abstract：:The type-VII ESX-1 secretion apparatus, encoded by the esx-1 genetic locus, is essential for the export of EsxA and EsxB, two major virulence factors of Mycobacterium tuberculosis. ESX-1 also requires the products of the unlinked espACD operon for optimal function and these proteins are considered integral parts of th...

journal_title：Molecular microbiology

authors： Chen JM,Zhang M,Rybniker J,Boy-Röttger S,Dhar N,Pojer F,Cole ST

更新日期：2013-09-01 00:00:00

abstract：:Almost all bacterial genomes contain DNA of viral origin, including functional prophages or degenerated phage elements. A frequent but often unnoted phenomenon is the spontaneous induction of prophage elements (SPI) even in the absence of an external stimulus. In this study, we have analyzed SPI of the large, degenera...

journal_title：Molecular microbiology

authors： Helfrich S,Pfeifer E,Krämer C,Sachs CC,Wiechert W,Kohlheyer D,Nöh K,Frunzke J

更新日期：2015-11-01 00:00:00

abstract：:In '5'-translational blockage', significantly reduced yields of proteins are synthesized in Escherichia coli when consecutive low-usage codons are inserted near translation starts of messages (with reduced or no effect when these same codons are inserted downstream). We tested the hypothesis that ribosomes encounterin...

journal_title：Molecular microbiology

authors： Gao W,Tyagi S,Kramer FR,Goldman E

更新日期：1997-08-01 00:00:00

abstract：:HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demon...

journal_title：Molecular microbiology

authors： Serrano JJ,de la Garza M,Moreno MA,Tovar R,León G,Tsutsumi V,Muñoz ML

更新日期：1994-02-01 00:00:00

abstract：:Understanding the mechanisms of polysialic acid synthesis in Escherichia coli K1 requires a molecular description of the polymerase complex. Since the number of potential models explaining polysialic acid assembly would be constrained if only one sialyltransferase were required for this process, the phenotypes of a si...

journal_title：Molecular microbiology

authors： Steenbergen SM,Vimr ER

更新日期：1990-04-01 00:00:00

abstract：:Understanding promoter regulation and signal-transduction systems in pathogenic mycobacteria is critical for uncovering the processes that govern interactions of these bacteria with the human host. In order to develop additional genetic tools for analysis of mycobacterial promoters, the xyIE gene from Pseudomonas was ...

journal_title：Molecular microbiology

authors： Curcic R,Dhandayuthapani S,Deretic V

更新日期：1994-09-01 00:00:00

abstract：:pAM beta 1 is a low-copy-number, promiscuous plasmid from Gram-positive bacteria that replicates by a unidirectional theta-type mode. Its replication is initiated by an original mechanism, involving the positive rate-limiting RepE protein. Here we show that the pAM beta 1-encoded CopF protein is involved in negative r...

journal_title：Molecular microbiology

authors： Le Chatelier E,Ehrlich SD,Jannière L

更新日期：1994-11-01 00:00:00

abstract：:We report here the cloning of the arginine repressor gene argR of Bacillus stearothermophilus and the characterization and purification to homogeneity of its product. The deduced amino acid sequence of the 16.8-kDa ArgR subunit shares 72% identity with its mesophilic homologue AhrC of Bacilus subtilis. Sequence analys...

journal_title：Molecular microbiology

authors： Dion M,Charlier D,Wang H,Gigot D,Savchenko A,Hallet JN,Glansdorff N,Sakanyan V

更新日期：1997-07-01 00:00:00

abstract：:Oligomerization of the initiator protein, DnaA, on the origin of replication (oriC) is crucial for initiation of DNA replication. Studies in Escherichia coli (Gram-negative) have revealed that binding of DnaA to ATP, but not hydrolysis of ATP, is sufficient to promote DnaA binding, oligomerization and DNA strand separ...

journal_title：Molecular microbiology

authors： Madiraju MV,Moomey M,Neuenschwander PF,Muniruzzaman S,Yamamoto K,Grimwade JE,Rajagopalan M

更新日期：2006-03-01 00:00:00

abstract：:The O-antigen of Salmonella lipopolysaccharide is a major antigenic determinant and its chemical composition forms the basis for Salmonella serotyping. Modifications of the O-antigen that can affect the serotype include those carried out by the products of glycosyltransferase operons (gtr), which are present on specif...

journal_title：Molecular microbiology

authors： Broadbent SE,Davies MR,van der Woude MW

更新日期：2010-07-01 00:00:00

abstract：:Type IV pili (T4P) are surface structures that undergo extension/retraction oscillations to generate cell motility. In Myxococcus xanthus, T4P are unipolarly localized and undergo pole-to-pole oscillations synchronously with cellular reversals. We investigated the mechanisms underlying these oscillations. We show that...

journal_title：Molecular microbiology

authors： Bulyha I,Schmidt C,Lenz P,Jakovljevic V,Höne A,Maier B,Hoppert M,Søgaard-Andersen L

更新日期：2009-11-01 00:00:00

abstract：:TOL plasmid pWW0 of Pseudomonas putida contains two operons that specify a pathway for the degradation of aromatic hydrocarbons. The upper pathway operon encodes the enzymes for the oxidation of toluene/xylenes to benzoate/toluates, and the metacleavage pathway operon encodes the enzymes for the further oxidation of t...

journal_title：Molecular microbiology

authors： Marqués S,Ramos JL

更新日期：1993-09-01 00:00:00

abstract：:Meningococcal gdhA, encoding the NADP-specific l-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promote...

journal_title：Molecular microbiology

authors： Pagliarulo C,Salvatore P,De Vitis LR,Colicchio R,Monaco C,Tredici M,Talà A,Bardaro M,Lavitola A,Bruni CB,Alifano P

更新日期：2004-03-01 00:00:00

abstract：:The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is almost undetectable at the end of the exponential phase. We discovered that this patt...

journal_title：Molecular microbiology

authors： O Cróinín T,Dorman CJ

更新日期：2007-10-01 00:00:00

abstract：:The esg locus is required for the formation of multicellular fruiting bodies and spores by the developmental bacterium Myxococcus xanthus. Studies have suggested that esg mutants are defective in the production of an essential signal (E-signal) used in cell-cell communication and that E-signalling is required for the ...

journal_title：Molecular microbiology

authors： Downard J,Toal D

更新日期：1995-04-01 00:00:00

abstract：:The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of trivalent metalloids, arsenite and antimonite. We have earlier shown that downregulation of LmAQP1 provides resistance to trivalent antimony compounds whereas increased expression of LmAQP1 in drug-resistant parasites can reverse the re...

journal_title：Molecular microbiology

authors： Figarella K,Uzcategui NL,Zhou Y,LeFurgey A,Ouellette M,Bhattacharjee H,Mukhopadhyay R

更新日期：2007-08-01 00:00:00

abstract：:Certain strains of Pichia acaciae and Wingea robertsiae (synonym Debaryomyces robertsiae) harbour extranuclear genetic elements that confer a killer phenotype to their host. Such killer plasmids (pPac1-2 of P. acaciae and pWR1A of W. robertsiae) were sequenced and compared with the zymocin encoding pGKL1 of Kluyveromy...

journal_title：Molecular microbiology

authors： Klassen R,Teichert S,Meinhardt F

更新日期：2004-07-01 00:00:00

abstract：:Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations in penicillin-binding proteins (PBPs) which reduce their affinity for beta-lactam antibiotics. PBP2x is one of the high-Mr PBPs which appears to be altered both in resistant clinical isolates, and in cefotaxime-resistant la...

journal_title：Molecular microbiology

authors： Laible G,Hakenbeck R,Sicard MA,Joris B,Ghuysen JM

更新日期：1989-10-01 00:00:00

abstract：:We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...

journal_title：Molecular microbiology

authors： Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

更新日期：2004-01-01 00:00:00

abstract：:The Ca(2+)-dependent protein phosphatase calcineurin is an important regulator of ion transporters from many organisms, including the Saccharomyces cerevisiae vacuolar Ca(2+)/H(+) exchanger Vcx1p. In yeast and plants, cation/H(+) exchangers are important in shaping cytosolic Ca(2+) levels involved in signal transducti...

journal_title：Molecular microbiology

authors： Pittman JK,Cheng NH,Shigaki T,Kunta M,Hirschi KD

更新日期：2004-11-01 00:00:00

abstract：:Burkholderia mallei, the aetiologic agent of glanders, causes a variety of illnesses in animals and humans ranging from occult infections to acute fulminating septicaemias. To better understand the role of lipopolysaccharide (LPS) in the pathogenesis of these diseases, studies were initiated to characterize the struct...

journal_title：Molecular microbiology

authors： Brett PJ,Burtnick MN,Snyder DS,Shannon JG,Azadi P,Gherardini FC

更新日期：2007-01-01 00:00:00

abstract：:Bacteroides fragilis toxin (BFT) is a protein secreted by enterotoxigenic (ETBF) strains of B. fragilis. BFT is synthesized as a proprotein (proBFT) that is predicted to be a lipoprotein and that is cleaved into two discrete fragments by a clostripain-like protease called fragipain (Fpn). In this study, we obtained ev...

journal_title：Molecular microbiology

authors： Pierce JV,Fellows JD,Anderson DE,Bernstein HD

更新日期：2020-09-29 00:00:00

abstract：:Rifampicin resistance, a defining attribute of multidrug-resistant tuberculosis, is conferred by mutations in the β subunit of RNA polymerase. Sequencing of rifampicin-resistant (RIF-R) clinical isolates of Mycobacterium tuberculosis revealed, in addition to RIF-R mutations, enrichment of potential compensatory mutati...

journal_title：Molecular microbiology

authors： Song T,Park Y,Shamputa IC,Seo S,Lee SY,Jeon HS,Choi H,Lee M,Glynne RJ,Barnes SW,Walker JR,Batalov S,Yusim K,Feng S,Tung CS,Theiler J,Via LE,Boshoff HI,Murakami KS,Korber B,Barry CE 3rd,Cho SN

更新日期：2014-03-01 00:00:00

abstract：:Transposon mutagenesis was used to isolate insertion mutants of the photosynthetic bacterium Rhodobacter capsulatus which were unable to grow under aerobic conditions in the dark on malate, succinate or fumarate as sole carbon sources. Of five mutants isolated, all were deficient in C4-dicarboxylate transport. However...

journal_title：Molecular microbiology

authors： Hamblin MJ,Shaw JG,Curson JP,Kelly DJ

更新日期：1990-09-01 00:00:00

abstract：:Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. aureus resulting in aggregation and thrombus formation is an important step in the pathogenesis of IE. Here, we report that the fibrinogen/fibronectin-binding proteins FnBPA and FnBPB are major platelet-activati...

journal_title：Molecular microbiology

authors： Fitzgerald JR,Loughman A,Keane F,Brennan M,Knobel M,Higgins J,Visai L,Speziale P,Cox D,Foster TJ

更新日期：2006-01-01 00:00:00

abstract：:Cell wall lipids of Mycobacterium tuberculosis containing multiple methylbranched fatty acids play critical roles in pathogenesis and thus offer targets for new antimycobacterial drugs. Mycocerosicacid synthase gene (mas) encodes the enzyme that produces one class of such acids. Seven mas-like genes (msls) were identi...

journal_title：Molecular microbiology

authors： Dubey VS,Sirakova TD,Kolattukudy PE

更新日期：2002-09-01 00:00:00

abstract：:Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P. aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruptio...

journal_title：Molecular microbiology

authors： Yang N,Ding S,Chen F,Zhang X,Xia Y,Di H,Cao Q,Deng X,Wu M,Wong CC,Tian XX,Yang CG,Zhao J,Lan L

更新日期：2015-05-01 00:00:00

abstract：:A model for the topology of the PhoE porin has been proposed according to which the polypeptide traverses the outer membrane sixteen times mostly as amphipathic beta-sheets, thereby exposing eight loops at the cell surface. Until now, no evidence has been obtained for the surface exposure of the third loop. Recently, ...

journal_title：Molecular microbiology

authors： Struyvé M,Visser J,Adriaanse H,Benz R,Tommassen J

更新日期：1993-01-01 00:00:00

abstract：:The chromosomal DpnII gene cassette of Streptococcus pneumoniae encodes two methyltransferases and an endonuclease. One methyltransferase acts on double-stranded and the other on single-stranded DNA. Two mRNAs are transcribed from the cassette. One, a SigA promoter transcript, includes all three genes; the other inclu...

journal_title：Molecular microbiology

authors： Lacks SA,Ayalew S,de la Campa AG,Greenberg B

更新日期：2000-03-01 00:00:00

abstract：:Deletion of gene Rv3676 in Mycobacterium tuberculosis coding for a transcription factor belonging to the cAMP receptor protein (CRP) family caused growth defects in laboratory medium, in bone marrow-derived macrophages and in a mouse model of tuberculosis. Transcript profiling of M. tuberculosis grown in vitro identif...

journal_title：Molecular microbiology

authors： Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

更新日期：2005-06-01 00:00:00