当前位置： SCI文献检索 > MOLECULAR MICROBIOLOGY期刊下所有文献 > The intrinsic ATPase activity of Mycobacterium tuberculosis DnaA promotes rapid oligomerization of DnaA on oriC.

The intrinsic ATPase activity of Mycobacterium tuberculosis DnaA promotes rapid oligomerization of DnaA on oriC.

Abstract：

:Oligomerization of the initiator protein, DnaA, on the origin of replication (oriC) is crucial for initiation of DNA replication. Studies in Escherichia coli (Gram-negative) have revealed that binding of DnaA to ATP, but not hydrolysis of ATP, is sufficient to promote DnaA binding, oligomerization and DNA strand separation. To begin understanding the initial events involved in the initiation of DNA replication in Mycobacterium tuberculosis (Gram-positive), we investigated interactions of M. tuberculosis DnaA (DnaA(TB)) with oriC using surface plasmon resonance in the presence of ATP and ADP. We provide evidence that, in contrast to what is observed in E. coli, ATPase activity of DnaA(TB) promoted rapid oligomerization on oriC. In support, we found that a recombinant mutant DnaA(TB) proficient in binding to ATP, but deficient in ATPase activity, did not oligomerize as rapidly. The corresponding mutation in the dnaA gene of M. tuberculosis resulted in non-viability, presumably due to a defect in oriC-DnaA interactions. Dimethy sulphate (DMS) footprinting experiments revealed that DnaA(TB) bound to DnaA boxes similarly with ATP or ADP. DnaA(TB) binding to individual DnaA boxes revealed that rapid oligomerization on oriC is triggered only after the initial interaction of DnaA with individual DnaA boxes. We propose that ATPase activity enables the DnaA protomers on oriC to rapidly form oligomeric complexes competent for replication initiation.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Madiraju MV,Moomey M,Neuenschwander PF,Muniruzzaman S,Yamamoto K,Grimwade JE,Rajagopalan M

doi

10.1111/j.1365-2958.2006.05068.x

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

1876-90

issue

6

eissn

0950-382X

issn

1365-2958

pii

MMI5068

journal_volume

59

pub_type

杂志文章

相关文献

MOLECULAR MICROBIOLOGY文献大全
  • Sulphated tyrosines mediate association of chemokines and Plasmodium vivax Duffy binding protein with the Duffy antigen/receptor for chemokines (DARC).

    abstract：:Plasmodium vivax is one of four Plasmodium species that cause human malaria. P. vivax and a related simian malaria parasite, Plasmodium knowlesi, invade erythrocytes by binding the Duffy antigen/receptor for chemokines (DARC) through their respective Duffy binding proteins. Here we show that tyrosines 30 and 41 of DAR...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2004.04478.x

    authors： Choe H,Moore MJ,Owens CM,Wright PL,Vasilieva N,Li W,Singh AP,Shakri R,Chitnis CE,Farzan M

    更新日期：2005-03-01 00:00:00

  • TFIIB and subunits of the SAGA complex are involved in transcriptional activation of phospholipid biosynthetic genes by the regulatory protein Ino2 in the yeast Saccharomyces cerevisiae.

    abstract：:In the yeast Saccharomyces cerevisiae, genes involved in phospholipid biosynthesis are activated by ICRE (inositol/choline-responsive element) up-stream motifs and the corresponding heterodimeric binding factor, Ino2 + Ino4. Both Ino2 and Ino4 contain basic helix-loop-helix (bHLH) domains required for ICRE binding, wh...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2003.03501.x

    authors： Dietz M,Heyken WT,Hoppen J,Geburtig S,Schüller HJ

    更新日期：2003-05-01 00:00:00

  • Limited variation and maintenance of tight genetic linkage characterize heteroallelic pilE recombination following DNA transformation of Neisseria gonorrhoeae.

    abstract：:Genetic recombination impacts on neisserial biology in two ways: (i) specific loci undergo rearrangement at high frequency leading to the formation of many different alleles; and (ii) Neisseria, being naturally competent for DNA transformation, provide a means to disseminate the novel alleles throughout a population. ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1996.5201047.x

    authors： Hill SA

    更新日期：1996-05-01 00:00:00

  • Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi.

    abstract：:Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1990.tb00651.x

    authors： Hinnebusch J,Bergström S,Barbour AG

    更新日期：1990-05-01 00:00:00

  • Regulation of the type IV pili molecular machine by dynamic localization of two motor proteins.

    abstract：:Type IV pili (T4P) are surface structures that undergo extension/retraction oscillations to generate cell motility. In Myxococcus xanthus, T4P are unipolarly localized and undergo pole-to-pole oscillations synchronously with cellular reversals. We investigated the mechanisms underlying these oscillations. We show that...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2009.06891.x

    authors： Bulyha I,Schmidt C,Lenz P,Jakovljevic V,Höne A,Maier B,Hoppert M,Søgaard-Andersen L

    更新日期：2009-11-01 00:00:00

  • Identification of structural and molecular determinants of the tyrosine-kinase Wzc and implications in capsular polysaccharide export.

    abstract：:Capsular polysaccharides are well-established virulence factors of pathogenic bacteria. Their biosynthesis and export are regulated within the transmembrane polysaccharide assembly machinery by the autophosphorylation of atypical tyrosine-kinases, named BY-kinases. However, the accurate functioning of these tyrosine-k...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2010.07291.x

    authors： Bechet E,Gruszczyk J,Terreux R,Gueguen-Chaignon V,Vigouroux A,Obadia B,Cozzone AJ,Nessler S,Grangeasse C

    更新日期：2010-09-01 00:00:00

  • Association of UBP1 to ribonucleoprotein complexes is regulated by interaction with the trypanosome ortholog of the human multifunctional P32 protein.

    abstract：:Regulation of gene expression in trypanosomatid parasitic protozoa is mainly achieved posttranscriptionally. RNA-binding proteins (RBPs) associate to 3' untranslated regions in mRNAs through dedicated domains such as the RNA recognition motif (RRM). Trypanosoma cruzi UBP1 (TcUBP1) is an RRM-type RBP involved in stabil...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.13090

    authors： Cassola A,Romaniuk MA,Primrose D,Cervini G,D'Orso I,Frasch AC

    更新日期：2015-09-01 00:00:00

  • Bre1 and Ubp8 regulate H2B mono-ubiquitination and the reversible yeast-hyphae transition in Candida albicans.

    abstract：:The reversible yeast-hyphae transition of the human fungal pathogen Candida albicans is tightly linked to its pathogenicity. In this study, we show that histone H2B mono-ubiquitination (H2Bub) at lysine 123 was maintained at a low level in the yeast state, whereas it increased significantly during yeast-to-hyphae tran...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.14619

    authors： Zhu W,Fan X,Zhao Q,Xu Y,Wang X,Chen J

    更新日期：2020-10-03 00:00:00

  • Apoptosis in yeast--mechanisms and benefits to a unicellular organism.

    abstract：:Initial observations that the budding yeast Saccharomyces cerevisiae can be induced to undergo a form of cell death exhibiting typical markers of apoptosis has led to the emergence of a thriving new field of research. Since this discovery, a number of conserved pro- and antiapoptotic proteins have been identified in y...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2958.2006.05486.x

    authors： Gourlay CW,Du W,Ayscough KR

    更新日期：2006-12-01 00:00:00

  • A RING-finger protein regulates carotenogenesis via proteolysis-independent ubiquitylation of a white collar-1-like activator.

    abstract：:Protein ubiquitylation plays a major role in the regulation of cellular processes mainly through proteasome-dependent degradation, although it has become increasingly clear that it is also involved in other processes. In the fungus Mucor circinelloides, blue light regulates carotene biosynthesis, with this response co...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2008.06470.x

    authors： Silva F,Navarro E,Peñaranda A,Murcia-Flores L,Torres-Martínez S,Garre V

    更新日期：2008-11-01 00:00:00

  • Lipooligosaccharide biosynthesis in Neisseria gonorrhoeae: cloning, identification and characterization of the alpha 1,5 heptosyltransferase I gene (rfaC)

    abstract：:The identical partial deep-core structure of Hep alpha 1-3Hep alpha 1-5KDO in Salmonella typhimurium LT2 LPS and Neisseria gonorrhoeae LOS enabled us to isolate a DNA fragment from N. gonorrhoeae that was able to complement the alpha 1,5 LOS heptosyltransferase defect in the S. typhimurium rfaC630 (SA1377) mutant. SDS...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1994.tb01300.x

    authors： Zhou D,Lee NG,Apicella MA

    更新日期：1994-11-01 00:00:00

  • A benzamide-dependent ftsZ mutant reveals residues crucial for Z-ring assembly.

    abstract：:In almost all bacteria, cell division is co-ordinated by the essential tubulin homologue FtsZ and represents an attractive but as yet unexploited target for new antibiotics. The benzamides, e.g. PC190723, are potent FtsZ inhibitors that have the potential to yield an important new class of antibiotic. However, the evo...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.13286

    authors： Adams DW,Wu LJ,Errington J

    更新日期：2016-03-01 00:00:00

  • Copper binding in IscA inhibits iron-sulphur cluster assembly in Escherichia coli.

    abstract：:Among the iron-sulphur cluster assembly proteins encoded by gene cluster iscSUA-hscBA-fdx in Escherichia coli, IscA has a unique and strong iron binding activity and can provide iron for iron-sulphur cluster assembly in proteins in vitro. Deletion of IscA and its paralogue SufA results in an E. coli mutant that fails ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.12676

    authors： Tan G,Cheng Z,Pang Y,Landry AP,Li J,Lu J,Ding H

    更新日期：2014-08-01 00:00:00

  • The SoxRS response of Escherichia coli is directly activated by redox-cycling drugs rather than by superoxide.

    abstract：:When Escherichia coli is exposed to redox-cycling drugs, its SoxR transcription factor is activated by oxidation of its [2Fe-2S] cluster. In aerobic cells these drugs generate superoxide, and because superoxide dismutase (SOD) is a member of the SoxRS regulon, superoxide was initially thought to be the activator of So...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2010.07520.x

    authors： Gu M,Imlay JA

    更新日期：2011-03-01 00:00:00

  • Characterization of a tightly controlled promoter of the halophilic archaeon Haloferax volcanii and its use in the analysis of the essential cct1 gene.

    abstract：:A system where archaeal gene expression could be controlled by simple manipulation of growth conditions would enable the construction of conditional lethal mutants in essential genes, and permit the controlled overproduction of proteins in their native host. As tools for the genetic manipulation of Haloferax volcanii ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2007.05980.x

    authors： Large A,Stamme C,Lange C,Duan Z,Allers T,Soppa J,Lund PA

    更新日期：2007-12-01 00:00:00

  • Molecular surveillance of the subtle septum: discovering a new mode of peptidoglycan synthesis in streptococci.

    abstract：:The process of septation requires precise temporal and spatial organization of penicillin binding proteins (PBPs) and associated proteins for the deposition of new cell wall material. In most bacteria, the filamentous protein FtsZ organises PBPs into assemblies at the midcell which then constrict inwards as peptidogly...

    journal_title：Molecular microbiology

    pub_type： 评论,杂志文章

    doi：10.1111/mmi.12756

    authors： Cadby IT,Lovering AL

    更新日期：2014-10-01 00:00:00

  • Menaquinone synthesis is critical for maintaining mycobacterial viability during exponential growth and recovery from non-replicating persistence.

    abstract：:Understanding the basis of bacterial persistence in latent infections is critical for eradication of tuberculosis. Analysis of Mycobacterium tuberculosis mRNA expression in an in vitro model of non-replicating persistence indicated that the bacilli require electron transport chain components and ATP synthesis for surv...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2009.06625.x

    authors： Dhiman RK,Mahapatra S,Slayden RA,Boyne ME,Lenaerts A,Hinshaw JC,Angala SK,Chatterjee D,Biswas K,Narayanasamy P,Kurosu M,Crick DC

    更新日期：2009-04-01 00:00:00

  • Genome-wide selection for increased copy number in Acinetobacter baylyi ADP1: locus and context-dependent variation in gene amplification.

    abstract：:Renewed interest in gene amplification stems from its importance in evolution and a variety of medical problems ranging from drug resistance to cancer. However, amplified DNA segments (amplicons) are not fully characterized in any organism. Here we report a novel Acinetobacter baylyi system for genome-wide studies. Am...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2011.07945.x

    authors： Seaton SC,Elliott KT,Cuff LE,Laniohan NS,Patel PR,Neidle EL

    更新日期：2012-02-01 00:00:00

  • SaPI mutations affecting replication and transfer and enabling autonomous replication in the absence of helper phage.

    abstract：:The SaPIs are chromosomal islands in staphylococci and other Gram-positive bacteria that carry genes for superantigens, virulence factors, resistance and certain metabolic functions. They have intimate relationships with certain temperate phages involving phage-induced excision, replication and efficient packaging in ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2007.06027.x

    authors： Ubeda C,Maiques E,Barry P,Matthews A,Tormo MA,Lasa I,Novick RP,Penadés JR

    更新日期：2008-02-01 00:00:00

  • A haem cofactor is required for redox and light signalling by the AppA protein of Rhodobacter sphaeroides.

    abstract：:The AppA protein of Rhodobacter sphaeroides is unique in its ability to sense and transmit redox signals as well as light signals. By functioning as antagonist to the PpsR transcriptional repressor, it regulates the expression of photosynthesis genes in response to these environmental stimuli. Here we show binding of ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2007.05724.x

    authors： Han Y,Meyer MH,Keusgen M,Klug G

    更新日期：2007-05-01 00:00:00

  • Interactions of Neisseria meningitidis with cells of the human meninges.

    abstract：:The interaction of Neisseria meningitidis with the meninges that surround and protect the brain is a pivotal event in the progression of bacterial meningitis. Two models of the human meninges were established in vitro, using (i) sections of fresh human brain and (ii) cultures of viable cells grown from human meningiom...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2000.01923.x

    authors： Hardy SJ,Christodoulides M,Weller RO,Heckels JE

    更新日期：2000-05-01 00:00:00

  • H-NOX regulation of c-di-GMP metabolism and biofilm formation in Legionella pneumophila.

    abstract：UNLABELLED:Haem Nitric oxide/OXygen (H-NOX) binding domains are a family of haemoprotein sensors that are widespread in bacterial genomes, but limited information is available on their function. Legionella pneumophila is the only prokaryote found, thus far, to encode two H-NOX proteins. This paper presents data support...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2010.07259.x

    authors： Carlson HK,Vance RE,Marletta MA

    更新日期：2010-08-01 00:00:00

  • The Escherichia coli unc transcription terminator enhances expression of uncC, encoding the epsilon subunit of F1-ATPase, from plasmids by stabilizing the transcript.

    abstract：:The effect of the unc transcription terminator on expression of uncC, encoding the epsilon subunit of Escherichia coli F1-ATPase, from plasmids was studied. The cloned sequence in pTK1 included the uncC ribosome binding site, the uncC structural gene, and the unc transcription terminator. The cloned region in pSD37 wa...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1990.tb02043.x

    authors： Patel AM,Dallmann HG,Skakoon EN,Kapala TD,Dunn SD

    更新日期：1990-11-01 00:00:00

  • 5'-Adenosinephosphosulphate reductase (CysH) protects Mycobacterium tuberculosis against free radicals during chronic infection phase in mice.

    abstract：:A major obstacle to tuberculosis (TB) control is the problem of chronic TB infection (CTBI). Here we report that 5'-adenosinephosphosulphate reductase (CysH), an enzyme essential for the production of reduced-sulphur-containing metabolites, is critical for Mycobacterium tuberculosis (Mtb) survival in chronic infection...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2006.05075.x

    authors： Senaratne RH,De Silva AD,Williams SJ,Mougous JD,Reader JR,Zhang T,Chan S,Sidders B,Lee DH,Chan J,Bertozzi CR,Riley LW

    更新日期：2006-03-01 00:00:00

  • Analysis of the effect of ppGpp on the ftsQAZ operon in Escherichia coli.

    abstract：:Escherichia coli loses its rod shape by inactivation of PBP2 (penicillin-binding protein 2), target of the beta-lactam mecillinam. Under these conditions, cell division is blocked in rich medium. Division in the absence of PBP2 activity is restored (and resistance to mecillinam is conferred) when the three cell divisi...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1998.00974.x

    authors： Navarro F,Robin A,D'Ari R,Joseleau-Petit D

    更新日期：1998-08-01 00:00:00

  • Recovery of adhesion to chondroitin-4-sulphate in Plasmodium falciparum varCSA disruption mutants by antigenically similar PfEMP1 variants.

    abstract：:Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed a...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2003.03595.x

    authors： Andrews KT,Pirrit LA,Przyborski JM,Sanchez CP,Sterkers Y,Ricken S,Wickert H,Lépolard C,Avril M,Scherf A,Gysin J,Lanzer M

    更新日期：2003-08-01 00:00:00

  • Genetic characterization of a Rhizobium meliloti lactose utilization locus.

    abstract：:We identified several linked genes of a lactose regulon in Rhizobium meliloti. These were lacZ, the structural gene for beta-galactosidase; lacR, the lactose repressor gene; and two genes encoding proteins of unknown function, lacW and lacX. Insertion mutants in lacW and lacZ belonged to a single genetic complementati...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1994.tb00298.x

    authors： Jelesko JG,Leigh JA

    更新日期：1994-01-01 00:00:00

  • Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli.

    abstract：:On the basis of the published N-terminal amino acid sequence of the soluble lytic transglycosylase 35 (Slt35) of Escherichia coli, an open reading frame (ORF) was cloned from the 60.8 min region of the E. coli chromosome. The nucleotide sequence of the ORF, containing a putative lipoprotein-processing site, was shown ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1995.tb02437.x

    authors： Ehlert K,Höltje JV,Templin MF

    更新日期：1995-05-01 00:00:00

  • mRNA processing by RNases J1 and J2 affects Bacillus subtilis gene expression on a global scale.

    abstract：:Ribonucleases J1 and J2 of Bacillus subtilis are evolutionarily conserved enzymes combining an endoribonucleolytic and a 5'-3' exoribonucleolytic activity in a single polypeptide. Their endoribonucleolytic cleavage specificity resembles that of RNase E, a key player in the processing and degradation of RNA in Escheric...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2008.06400.x

    authors： Mäder U,Zig L,Kretschmer J,Homuth G,Putzer H

    更新日期：2008-10-01 00:00:00

  • Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation.

    abstract：:Poly(A) polymerase I (PAP I) of Escherichia coli is a member of the nucleotidyltransferase (Ntr) superfamily that includes the eukaryotic PAPs and all the known tRNA CCA-adding enzymes. Five highly conserved aspartic acids in the putative catalytic site of PAP I were changed to either alanine or proline, demonstrating...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1999.01394.x

    authors： Raynal LC,Carpousis AJ

    更新日期：1999-05-01 00:00:00