Abstract:
:The effect of the unc transcription terminator on expression of uncC, encoding the epsilon subunit of Escherichia coli F1-ATPase, from plasmids was studied. The cloned sequence in pTK1 included the uncC ribosome binding site, the uncC structural gene, and the unc transcription terminator. The cloned region in pSD37 was similar, but lacked the unc transcription terminator. Transformants carrying pTK1 produced the epsilon subunit of F1-ATPase, encoded by uncC, in 10-fold greater abundance than transformants carrying pSD37. Northern blots revealed similar differences in the steady-state uncC mRNA levels. The half-life of the message transcribed from pTK1 was 90-100 seconds, while that from pSD37 was 25-30 seconds. These studies indicate the importance of message stabilization through features at the 3' end of the transcript in ensuring adequate production of epsilon. We have exploited this stabilization to develop a simple, efficient, and gentle method of purifying the overproduced epsilon subunit.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Patel AM,Dallmann HG,Skakoon EN,Kapala TD,Dunn SDdoi
10.1111/j.1365-2958.1990.tb02043.xsubject
Has Abstractpub_date
1990-11-01 00:00:00pages
1941-6issue
11eissn
0950-382Xissn
1365-2958journal_volume
4pub_type
杂志文章abstract::The sex pheromone cAM373 of Enterococcus faecalis and the related staph-cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably se...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
abstract::Clp ATPases, which include the ubiquitous HSP100 family, are classified according to their structural features and sequence similarities. During the course of the Bacillus subtilis genome sequencing project, we identified a gene encoding a new member of the HSP100 family. We designated this protein ClpE, as it is the ...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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更新日期:2007-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Molecular microbiology
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更新日期:2020-09-04 00:00:00
abstract::Pseudomonas aeruginosa OprD is a 420-amino-acid protein that facilitates the uptake of basic amino acids, imipenem and gluconate across the outer membrane. OprD was the first specific porin that could be aligned with members of the non-specific porin super-family. Utilizing multiple alignments in conjugation with stru...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
abstract::Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility i...
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更新日期:2010-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 评论,杂志文章
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更新日期:2015-07-01 00:00:00
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更新日期:1991-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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更新日期:1998-06-01 00:00:00
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更新日期:2020-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1997-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02301.x
更新日期:2001-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12403
更新日期:2013-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mmi.13147
更新日期:2015-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
abstract::The localization of SeqA, thymidylate synthase, DnaB (helicase) and the DNA polymerase components alpha and tau, has been studied by immunofluorescence microscopy. The origin has been labelled through GFP-LacI bound near oriC. SeqA was located in the cell centre for one replication factory (RF) and at 1/4 and 3/4 posi...
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pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00