Abstract:
:Pseudomonas aeruginosa OprD is a 420-amino-acid protein that facilitates the uptake of basic amino acids, imipenem and gluconate across the outer membrane. OprD was the first specific porin that could be aligned with members of the non-specific porin super-family. Utilizing multiple alignments in conjugation with structure predictions and amphipathicity calculations, an OprD-topology model was proposed. Sixteen beta-strands were predicted, connected by short loops at the periplasmic side. The eight external loops were of variable length but tended to be much longer than the periplasmic ones. Polymerase chain reaction (PCR)-based site-specific mutagenesis was performed to delete separately short stretches (4-8 amino acid residues) from each of the predicted external loops. The mutants with deletions in the predicted external loops L1, L2, L5, L6, L7 and L8 were tolerated in both Escherichia coli and P. aeruginosa. The expressed mutant proteins maintained substantial resistance to trypsin treatment in the context of isolated outer membranes. Proteins with deletions in loops L1, L5, L6, L7 and L8 reconstituted similar imipenem supersusceptibility in a P. aeruginosa OprD:: omega background. The L2-deletion mutant only partially reconstituted super-susceptibility, suggesting that loop L2 is involved in imipenem binding. These data were generally consistent with the topology model.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Huang H,Jeanteur D,Pattus F,Hancock REdoi
10.1111/j.1365-2958.1995.tb02319.xsubject
Has Abstractpub_date
1995-06-01 00:00:00pages
931-41issue
5eissn
0950-382Xissn
1365-2958journal_volume
16pub_type
杂志文章abstract::Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is exposed on the surface of infected erythrocytes where it both acts as an important pathogenicity factor in malaria and undergoes antigenic variation as a means of immune evasion. Because the mammalian erythrocyte lacks a protein secretory machinery ther...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01647.x
更新日期:1999-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1997.2231590.x
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更新日期:2000-08-01 00:00:00
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更新日期:2002-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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pub_type: 评论,杂志文章
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pub_type: 杂志文章
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journal_title:Molecular microbiology
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更新日期:1996-03-01 00:00:00
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更新日期:2004-04-01 00:00:00
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更新日期:2014-10-13 00:00:00
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journal_title:Molecular microbiology
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更新日期:2003-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12840
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pub_type: 杂志文章,评审
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更新日期:1998-01-01 00:00:00
abstract::For malaria to be transmitted, the Plasmodium falciparum parasite must invade an erythrocyte and undergo gametocytogenesis. When mature intraerythrocytic gametocytes are taken up in a blood meal by a mosquito they emerge as gametes and, once fertilized, continue to differentiate into infectious sporozoites. One of the...
journal_title:Molecular microbiology
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更新日期:2002-06-01 00:00:00
abstract::RecA2278-5 is a mutant RecA protein (RecAmut) bearing two amino acid substitutions, Gly-278 to Thr and Val-275 to Phe, in the alpha-helix H of the C-terminal subdomain of the protein. RecA2278-5 mutant cells are unusual in that they are thermosensitive for recombination but almost normal for DNA repair of UV damage an...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1997.1961557.x
更新日期:1997-01-01 00:00:00