Physical-chemical plant-derived signals induce differentiation in Ustilago maydis.

Abstract:

:Ustilago maydis is able to initiate pathogenic development after fusion of two haploid cells with different mating type. On the maize leaf surface, the resulting dikaryon switches to filamentous growth, differentiates appressoria and penetrates the host. Here, we report on the plant signals required for filament formation and appressorium development in U. maydis. In vitro, hydroxy-fatty acids stimulate filament formation via the induction of pheromone genes and this signal can be bypassed by genetically activating the downstream MAP kinase module. Hydrophobicity also induces filaments and these resemble the dikaryotic filaments formed on the plant surface. With the help of a marker gene that is specifically expressed in the tip cell of those hyphae that have formed an appressorium, hydrophobicity is shown to be essential for appressorium development in vitro. Hydroxy-fatty acids or a cutin monomer mixture isolated from maize leaves have a stimulatory role when a hydrophobic surface is provided. Our results suggest that the early phase of communication between U. maydis and its host plant is governed by two different stimuli.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Mendoza-Mendoza A,Berndt P,Djamei A,Weise C,Linne U,Marahiel M,Vranes M,Kämper J,Kahmann R

doi

10.1111/j.1365-2958.2008.06567.x

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

895-911

issue

4

eissn

0950-382X

issn

1365-2958

pii

MMI6567

journal_volume

71

pub_type

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