The transmembrane domains of the sensor kinase KdpD of Escherichia coli are not essential for sensing K+ limitation.

abstract：:Eeyarestatin 1 (ES1) is an inhibitor of endoplasmic reticulum (ER) associated protein degradation, Sec61-dependent Ca2+ homeostasis and protein translocation into the ER. Recently, evidence was presented showing that a smaller analog of ES1, ES24, targets the Sec61-translocon, and captures it in an open conformation t...

journal_title：Molecular microbiology

authors： Steenhuis M,Koningstein GM,Oswald J,Pick T,O'Keefe S,Koch HG,Cavalié A,Whitehead RC,Swanton E,High S,Luirink J

更新日期：2020-08-15 00:00:00

abstract：:The adaptation of microorganisms to different temperatures is an advantage in habitats with steadily changing conditions and raises the question about temperature sensing. Here we show that in the filamentous fungus Aspergillus nidulans, the hybrid histidine kinase TcsB and phytochrome are involved in temperature-indu...

journal_title：Molecular microbiology

authors： Yu Z,Ali A,Igbalajobi OA,Streng C,Leister K,Krauß N,Lamparter T,Fischer R

更新日期：2019-12-01 00:00:00

abstract：:Trans-acting small regulatory RNAs (sRNAs) are key players in the regulation of gene expression in bacteria. There are hundreds of different sRNAs in a typical bacterium, which in contrast to eukaryotic microRNAs are more heterogeneous in length, sequence composition, and secondary structure. The vast majority of sRNA...

journal_title：Molecular microbiology

authors： Georg J,Lalaouna D,Hou S,Lott SC,Caldelari I,Marzi S,Hess WR,Romby P

更新日期：2020-03-01 00:00:00

abstract：:The crystal structures of two active forms of dissimilatory sulphite reductase (Dsr) from Desulfovibrio gigas, Dsr-I and Dsr-II, are compared at 1.76 and 2.05 Å resolution respectively. The dimeric α2β2γ2 structure of Dsr-I contains eight [4Fe-4S] clusters, two saddle-shaped sirohaems and two flat sirohydrochlorins. I...

journal_title：Molecular microbiology

authors： Hsieh YC,Liu MY,Wang VC,Chiang YL,Liu EH,Wu WG,Chan SI,Chen CJ

更新日期：2010-12-01 00:00:00

abstract：:We used the LacO/GFP-LacI system to label and visualize the IncP beta plasmid R751 fluorescently during conjugative transfer between live donor and recipient bacteria. Comparisons of R751 in conjugative and non-conjugative conditions have allowed us to identify key localizations and movements associated with the initi...

journal_title：Molecular microbiology

authors： Lawley TD,Gordon GS,Wright A,Taylor DE

更新日期：2002-05-01 00:00:00

abstract：:Genetic exchange by natural transformation in Streptococcus pneumoniae occurs in a cell-density dependent process and is initiated by a small extracellular signalling molecule, the competence-stimulating peptide (CSP). comC, the gene for this peptide, has previously been identified and encodes a 44 amino acid pre-pept...

journal_title：Molecular microbiology

authors： Cheng Q,Campbell EA,Naughton AM,Johnson S,Masure HR

更新日期：1997-02-01 00:00:00

abstract：:The Frz pathway of Myxococcus xanthus controls cell reversal frequency to support directional motility during swarming and fruiting body formation. Previously, we showed that phosphorylation of the response regulator FrzZ correlates with reversal frequencies, suggesting that this activity represents the output of the ...

journal_title：Molecular microbiology

authors： Kaimer C,Zusman DR

更新日期：2016-04-01 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00

abstract：:Heteroduplexes between the DNA molecules of 12 lambdoid phages were analysed by electron microscopy. The positions of the regions of base sequence homology between the DNA molecules divide them into 35 segments, most of which have a number of alternative forms (alleles), which in general must be functionally homologou...

journal_title：Molecular microbiology

authors： Highton PJ,Chang Y,Myers RJ

更新日期：1990-08-01 00:00:00

abstract：:SigD and SigE (Salmonella invasion gene) are proteins needed for optimal invasion of Salmonella typhimurium into eukaryotic cells in vitro. SigD is a secreted protein and SigE is a putative chaperone required for SigD stability and/or secretion. SigD is secreted by a type III secretion apparatus encoded within a patho...

journal_title：Molecular microbiology

authors： Darwin KH,Miller VL

更新日期：2000-02-01 00:00:00

abstract：:The DnaA protein, which initiates chromosomal replication in Escherichia coli, is negatively regulated by both the sliding clamp of DNA polymerase III holoenzyme and the IdaB protein. We have found that, when the amount of minichromosome is limited in an in vitro replication system, minichromosomal replication-stimula...

journal_title：Molecular microbiology

authors： Su'etsugu M,Kawakami H,Kurokawa K,Kubota T,Takata M,Katayama T

更新日期：2001-04-01 00:00:00

abstract：:The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in certain bean cultivars. Previous studies have shown that when PPHGI-1 ...

journal_title：Molecular microbiology

authors： Neale HC,Jackson RW,Preston GM,Arnold DL

更新日期：2018-11-01 00:00:00

abstract：:SpollE is an integral membrane protein that governs the establishment of cell-specific gene transcription during the process of sporulation in Bacillus subtilis. Synthesis of SpollE commences shortly after the onset of sporulation, after which the protein localizes at sites of potential cell division near both ends of...

journal_title：Molecular microbiology

authors： Levin PA,Losick R,Stragier P,Arigoni F

更新日期：1997-09-01 00:00:00

abstract：:Sphingomyelin is the main sphingolipid in Trypanosoma brucei, the causative agent of African sleeping sickness. In vitro and in vivo characterization of the T. brucei neutral sphingomyelinase demonstrates that it is directly involved in sphingomyelin catabolism. Gene knockout studies in the bloodstream form of the par...

journal_title：Molecular microbiology

authors： Young SA,Smith TK

更新日期：2010-06-01 00:00:00

abstract：:Certain strains of Pichia acaciae and Wingea robertsiae (synonym Debaryomyces robertsiae) harbour extranuclear genetic elements that confer a killer phenotype to their host. Such killer plasmids (pPac1-2 of P. acaciae and pWR1A of W. robertsiae) were sequenced and compared with the zymocin encoding pGKL1 of Kluyveromy...

journal_title：Molecular microbiology

authors： Klassen R,Teichert S,Meinhardt F

更新日期：2004-07-01 00:00:00

abstract：:By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open readin...

journal_title：Molecular microbiology

authors： Torres L,Martín H,García-Saez MI,Arroyo J,Molina M,Sánchez M,Nombela C

更新日期：1991-11-01 00:00:00

abstract：:The distribution of PBP5, the major D,D-carboxypeptidase in Escherichia coli, was mapped by immunolabelling and by visualization of GFP fusion proteins in wild-type cells and in mutants lacking one or more D,D-carboxypeptidases. In addition to being scattered around the lateral envelope, PBP5 was also concentrated at ...

journal_title：Molecular microbiology

authors： Potluri L,Karczmarek A,Verheul J,Piette A,Wilkin JM,Werth N,Banzhaf M,Vollmer W,Young KD,Nguyen-Distèche M,den Blaauwen T

更新日期：2010-07-01 00:00:00

abstract：:Biofilms are communities of microbial cells that are encased in a self-produced, polymeric matrix and are adherent to a surface. For several species of bacteria, an enhanced ability to form biofilms has been linked with an increased capability to produce exopolymers. To identify exopolymers of Bacillus subtilis that c...

journal_title：Molecular microbiology

authors： Stanley NR,Lazazzera BA

更新日期：2005-08-01 00:00:00

abstract：:MexAB-OprM is a multidrug efflux system that contributes to intrinsic and acquired multidrug resistance in Pseudomonas aeruginosa, the latter as a result of mutational hyperexpression of the mexAB-oprM operon. While efflux gene hyperexpression typically results from mutations in the linked mexR repressor gene, it also...

journal_title：Molecular microbiology

authors： Cao L,Srikumar R,Poole K

更新日期：2004-09-01 00:00:00

abstract：:The aroA gene from Pasteurella multocida was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18. The nucleotide sequence of the P. multocida aroA gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology ...

journal_title：Molecular microbiology

authors： Homchampa P,Strugnell RA,Adler B

更新日期：1992-12-01 00:00:00

abstract：:A gene conferring low-level isoniazid (INH) resistance on Mycobacterium smegmatis was isolated from a cosmid library of the genome of an INH-resistant Mycobacterium bovis strain. The gene had good homology with ahpC, the product of which is a subunit of alkyl hydroperoxide reductase, and also with a family of thiol-sp...

journal_title：Molecular microbiology

authors： Wilson TM,Collins DM

更新日期：1996-03-01 00:00:00

abstract：:The FNR protein of Escherichia coli is a regulatory protein that activates the transcription of its target genes in response to oxygen limitation. Site-directed mutagenesis was used to show that a 28-residue N-terminal segment containing three cysteines is essential for normal FNR function. The cysteine residue which ...

journal_title：Molecular microbiology

authors： Spiro S,Guest JR

更新日期：1988-11-01 00:00:00

abstract：:Transcription of flagellar genes in Caulobacter crecentus is programmed to occur during the predivisional stage of the cell cycle. The mechanism of activation of Class II flagellar genes, the highest identified genes in the Caulobacter flagellar hierarchy, is unknown. As a step toward understanding this process, we ha...

journal_title：Molecular microbiology

authors： Stephens CM,Shapiro L

更新日期：1993-09-01 00:00:00

abstract：:Haemophilus influenzae is a human-specific pathogen and a major source of morbidity worldwide. Infection with this organism begins with colonization of the nasopharynx, a process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein is the major adhesin ex-pressed by a subset of...

journal_title：Molecular microbiology

authors： Laarmann S,Cutter D,Juehne T,Barenkamp SJ,St Geme JW

更新日期：2002-11-01 00:00:00

abstract：:Fourteen Rhodobacter capsulatus mutants unable to grow with xanthine as sole nitrogen source were isolated by random Tn5 mutagenesis. Five of these Tn5 insertions were mapped within two adjacent chromosomal EcoRI fragments hybridizing to oligonucleotides synthesized according to conserved amino acid sequences of eukar...

journal_title：Molecular microbiology

authors： Leimkühler S,Kern M,Solomon PS,McEwan AG,Schwarz G,Mendel RR,Klipp W

更新日期：1998-02-01 00:00:00

abstract：:Sequence determination of a region downstream from the vanXYc gene in Enterococcus gallinarum BM4174 revealed an open reading frame, designated vanT, that encodes a 698-amino-acid polypeptide with an amino-terminal domain containing 10 predicted transmembrane segments. The protein contained a highly conserved pyridoxa...

journal_title：Molecular microbiology

authors： Arias CA,Martín-Martinez M,Blundell TL,Arthur M,Courvalin P,Reynolds PE

更新日期：1999-03-01 00:00:00

abstract：:Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient molecules, perhaps even predating the existence of the translation machinery. Albeit old, these mole...

journal_title：Molecular microbiology

authors： Katz A,Elgamal S,Rajkovic A,Ibba M

更新日期：2016-08-01 00:00:00

abstract：:The human fungal pathogen Candida albicans switches its morphology from yeast to hyphal forms. The morphological transition may render C. albicans virulent. Several signaling cascades, including those of the cyclic AMP-protein kinase A and mitogen-activated protein kinase pathways, are responsible for morphogenesis. I...

journal_title：Molecular microbiology

authors： Kim SW,Joo YJ,Chun YJ,Park YK,Kim J

更新日期：2019-09-01 00:00:00

abstract：:Two distinct merR genes, which regulate expression of the mercuric ion resistance gene (mer), of Thiobacillus ferrooxidans strain E-15 have been cloned, sequenced and termed merR1 and merR2. As a result of gene walking around two merR genes, it was found that these two genes were quite close in distance. The nucleotid...

journal_title：Molecular microbiology

authors： Inoue C,Sugawara K,Kusano T

更新日期：1991-11-01 00:00:00

abstract：:Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) ...

journal_title：Molecular microbiology

authors： Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JS

更新日期：2009-11-01 00:00:00