Abstract:
:Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) binding protein and contributes to bacterial resistance to phagocytosis and survival in the bloodstream. Interestingly, prior exposure to plasma at low concentrations was sufficient to enhance bacterial survival in the circulation. Calcium ions at physiological plasma concentrations induced maturation of AtlA from the 104-90 kDa isoform resulting in increased Fn binding and resistance to phagocytosis. An isogenic mutant strain defective in AtlA expression exhibited reduced survival and virulence when tested in a rat model of IE compared with the wild-type and complemented strains. The data presented suggest that plasma components utilized by S. mutans enhanced survival in the circulation and AtlA is a virulence factor associated with infective endocarditis.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JSdoi
10.1111/j.1365-2958.2009.06903.xsubject
Has Abstractpub_date
2009-11-01 00:00:00pages
888-902issue
4eissn
0950-382Xissn
1365-2958pii
MMI6903journal_volume
74pub_type
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