Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active delta-endotoxin gene of Bacillus thuringiensis aizawai 7.29.

Abstract:

:The nucleotide sequence of a 2711bp DNA segment which contains the N-terminal coding sequence and the 5' flanking region of a crystal protein gene (bta) from Bacillus thuringiensis subsp. aizawai 7.29 has been determined. The coding region encodes an 824 amino-acid polypeptide corresponding to a carboxy-terminally truncated delta-endotoxin specifically active against the cotton leaf worm Spodoptera littoralis. Comparison of the deduced amino acid sequence of the bta gene with that of the 4.5, 5.3 and 6.6 kb classes of lepidopteran-active delta-endotoxins revealed that the Bta sequence contains a very high level of amino acid substitutions in the N-terminal part of the protoxin molecule. The substitutions are grouped in several highly variable segments separated by highly conserved regions. These conserved domains are also present in the dipteran- and coleopteran-active delta-endotoxins. The control region of the bta gene shows considerable DNA identity with the control regions of the other lepidopteran-active genes. Deletions of the 3' region of the gene were carried out and the toxic fraction of the bta delta-endotoxin was identified with the N-terminal half of the molecule.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Sanchis V,Lereclus D,Menou G,Chaufaux J,Guo S,Lecadet MM

doi

10.1111/j.1365-2958.1989.tb01812.x

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

229-38

issue

2

eissn

0950-382X

issn

1365-2958

journal_volume

3

pub_type

杂志文章
  • Mechanisms for maintaining cell shape in rod-shaped Gram-negative bacteria.

    abstract::For the rod-shaped Gram-negative bacterium Escherichia coli, changes in cell shape have critical consequences for motility, immune system evasion, proliferation and adhesion. For most bacteria, the peptidoglycan cell wall is both necessary and sufficient to determine cell shape. However, how the synthesis machinery as...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2011.07616.x

    authors: Furchtgott L,Wingreen NS,Huang KC

    更新日期:2011-07-01 00:00:00

  • Messenger RNA release from ribosomes during 5'-translational blockage by consecutive low-usage arginine but not leucine codons in Escherichia coli.

    abstract::In '5'-translational blockage', significantly reduced yields of proteins are synthesized in Escherichia coli when consecutive low-usage codons are inserted near translation starts of messages (with reduced or no effect when these same codons are inserted downstream). We tested the hypothesis that ribosomes encounterin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.5081871.x

    authors: Gao W,Tyagi S,Kramer FR,Goldman E

    更新日期:1997-08-01 00:00:00

  • Involvement of the amino-terminal phosphorylation module of UhpA in activation of uhpT transcription in Escherichia coli.

    abstract::The UhpA protein is required for expression of the sugar phosphate transporter UhpT in Escherichia coli and is regulated by phosphate transfer from the transmembrane UhpBC sensor kinase complex. UhpA action requires the sensor kinase complex and the site of phosphorylation, Asp-54, under normal conditions, but not whe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.4021765.x

    authors: Webber CA,Kadner RJ

    更新日期:1997-06-01 00:00:00

  • Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential for Plasmodium yoelii liver-stage development.

    abstract::Malaria parasites scavenge nutrients from their host but also harbour enzymatic pathways for de novo macromolecule synthesis. One such pathway is apicoplast-targeted type II fatty acid synthesis, which is essential for late liver-stage development in rodent malaria. It is likely that fatty acids synthesized in the api...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12485

    authors: Lindner SE,Sartain MJ,Hayes K,Harupa A,Moritz RL,Kappe SH,Vaughan AM

    更新日期:2014-02-01 00:00:00

  • Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442.

    abstract::The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2010.07450.x

    authors: Galán B,Dinjaski N,Maestro B,de Eugenio LI,Escapa IF,Sanz JM,García JL,Prieto MA

    更新日期:2011-01-01 00:00:00

  • Biochemical characterization of Leishmania major aquaglyceroporin LmAQP1: possible role in volume regulation and osmotaxis.

    abstract::The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of trivalent metalloids, arsenite and antimonite. We have earlier shown that downregulation of LmAQP1 provides resistance to trivalent antimony compounds whereas increased expression of LmAQP1 in drug-resistant parasites can reverse the re...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05845.x

    authors: Figarella K,Uzcategui NL,Zhou Y,LeFurgey A,Ouellette M,Bhattacharjee H,Mukhopadhyay R

    更新日期:2007-08-01 00:00:00

  • A co-activator of nitrogen-regulated transcription in Saccharomyces cerevisiae.

    abstract::In Saccharomyces cerevisiae, the transcription factors Gln3p and Nil1p of the GATA family play a determinant role in expression of genes that are subject to nitrogen catabolite repression. Here we report the isolation of a new yeast mutant, gan1-1, exhibiting dramatically decreased NAD-linked glutamate dehydrogenase (...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1999.01187.x

    authors: Soussi-Boudekou S,André B

    更新日期:1999-02-01 00:00:00

  • Mismatch repair and the regulation of phase variation in Neisseria meningitidis.

    abstract::Neisseria meningitidis controls the expression of several genes involved in host adaptation by a process known as phase variation. The phase variation frequency of haemoglobin (Hb) receptors among clinical isolates of serogroups A, B and C differed drastically, ranging from approximately 10(-6) to 10(-2) cfu-1. Freque...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02408.x

    authors: Richardson AR,Stojiljkovic I

    更新日期:2001-05-01 00:00:00

  • Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by alkaline phosphatase fusions.

    abstract::The cytochrome d complex of Escherichia coli is a heterodimer located in the bacterial cytoplasmic membrane, where it functions as a terminal oxidase of the aerobic respiratory chain. The topology of each of the two subunits of the cytochrome d complex was analysed by the genetic method involving alkaline phosphatase ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1991.tb02097.x

    authors: Newton G,Yun CH,Gennis RB

    更新日期:1991-10-01 00:00:00

  • Genetic identification of exported proteins in Streptococcus pneumoniae.

    abstract::A strategy was developed to mutate and genetically identify exported proteins in Streptococcus pneumoniae. Vectors were created and used to screen pneumococcal DNA in Escherichia coli and S. pneumoniae for translational gene fusions to alkaline phosphatase (PhoA). Twenty five PhoA+ pneumococcal mutants were isolated a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb01233.x

    authors: Pearce BJ,Yin YB,Masure HR

    更新日期:1993-09-01 00:00:00

  • Carbohydrate-binding agents act as potent trypanocidals that elicit modifications in VSG glycosylation and reduced virulence in Trypanosoma brucei.

    abstract::The surface of Trypanosoma brucei is covered by a dense coat of glycosylphosphatidylinositol-anchored glycoproteins. The major component is the variant surface glycoprotein (VSG) which is glycosylated by both paucimannose and oligomannose N-glycans. Surface glycans are poorly accessible and killing mediated by peptide...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12359

    authors: Castillo-Acosta VM,Vidal AE,Ruiz-Pérez LM,Van Damme EJ,Igarashi Y,Balzarini J,González-Pacanowska D

    更新日期:2013-11-01 00:00:00

  • Identification of a multifunctional protein, PhaM, that determines number, surface to volume ratio, subcellular localization and distribution to daughter cells of poly(3-hydroxybutyrate), PHB, granules in Ralstonia eutropha H16.

    abstract::A two-hybrid approach was applied to screen for proteins with the ability to interact with PHB synthase (PhaC1) of Ralstonia eutropha. The H16_A0141 gene (phaM) was identified in the majority of positive clones. PhaM (26.6 kDa) strongly interacted with PhaC1 and with phasin PhaP5 but not with PhaP1 or other PHB granul...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2011.07869.x

    authors: Pfeiffer D,Wahl A,Jendrossek D

    更新日期:2011-11-01 00:00:00

  • Evasion of killing by human antibody and complement through multiple variations in the surface oligosaccharide of Haemophilus influenzae.

    abstract::The lipopolysaccharide (LPS) of H. influenzae is highly variable. Much of the structural diversity is derived from phase variation, or high frequency on-off switching, of molecules attached during LPS biosynthesis. In this study, we examined the dynamics of LPS phase variation following exposure to human serum as a so...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12214

    authors: Clark SE,Eichelberger KR,Weiser JN

    更新日期:2013-05-01 00:00:00

  • The metabolic regulator CodY links Listeria monocytogenes metabolism to virulence by directly activating the virulence regulatory gene prfA.

    abstract::Metabolic adaptations are critical to the ability of bacterial pathogens to grow within host cells and are normally preceded by sensing of host-specific metabolic signals, which in turn can influence the pathogen's virulence state. Previously, we reported that the intracellular bacterial pathogen Listeria monocytogene...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12890

    authors: Lobel L,Sigal N,Borovok I,Belitsky BR,Sonenshein AL,Herskovits AA

    更新日期:2015-02-01 00:00:00

  • Green fluorescent protein as a new expression marker in mycobacteria.

    abstract::This study describes the use and the advantages of the green fluorescent protein (GFP) as a reporter molecule for mycobacteria. The gfp gene from Aequorea victoria was placed under the control of the hsp60 promoter in the shuttle vector pGFM-11. The gfp expression in the recombinant Mycobacterium smegmatis and BCG was...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1995.mmi_17050913.x

    authors: Kremer L,Baulard A,Estaquier J,Poulain-Godefroy O,Locht C

    更新日期:1995-09-01 00:00:00

  • RloC: a wobble nucleotide-excising and zinc-responsive bacterial tRNase.

    abstract:SUMMARY:The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06387.x

    authors: Davidov E,Kaufmann G

    更新日期:2008-09-01 00:00:00

  • The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system.

    abstract::In Pseudomonas aeruginosa three type VI secretion systems (T6SSs) coexist, called H1- to H3-T6SSs. Several T6SS components are proposed to be part of a macromolecular complex resembling the bacteriophage tail. The T6SS protein HsiE1 (TagJ) is unique to the H1-T6SS and absent from the H2- and H3-T6SSs. We demonstrate t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2012.08204.x

    authors: Lossi NS,Manoli E,Simpson P,Jones C,Hui K,Dajani R,Coulthurst SJ,Freemont P,Filloux A

    更新日期:2012-10-01 00:00:00

  • The Caulobacter crescentus polar organelle development protein PodJ is differentially localized and is required for polar targeting of the PleC development regulator.

    abstract::Regulation of polar development and cell division in Caulobacter crescentus relies on the dynamic localization of several proteins to cell poles at specific stages of the cell cycle. The polar organelle development protein, PodJ, is required for the synthesis of the adhesive holdfast and pili. Here we show the cell cy...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03349.x

    authors: Hinz AJ,Larson DE,Smith CS,Brun YV

    更新日期:2003-02-01 00:00:00

  • The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in the defence against DNA damage.

    abstract::We have cloned, characterized and inactivated genes encoding putative UspA paralogues in Escherichia coli. The yecG (uspC), yiiT (uspD) and ydaA (uspE) genes were demonstrated to encode protein pro-ducts and these were mapped to spots in the E. coli proteomic database. Expression analysis using chromosomal transcripti...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2002.02720.x

    authors: Gustavsson N,Diez A,Nyström T

    更新日期:2002-01-01 00:00:00

  • Ubiquitin-Yop hybrids as probes for post-translational transport by the Yersinia type III secretion pathway.

    abstract::Yersinia enterocolitica uses type III secretion to transport Yop proteins into the cytoplasm of host cells. Previous work generated hypotheses for both co- and post-translational transport mechanisms in the Yersinia type III pathway. Here, we used ubiquitin (Ub) and UBP1, the Ub-specific protease, to examine whether Y...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05793.x

    authors: Quenee LE,Schneewind O

    更新日期:2007-07-01 00:00:00

  • In Candida albicans hyphae, Sec2p is physically associated with SEC2 mRNA on secretory vesicles.

    abstract::Candida albicans hyphae grow in a highly polarized fashion from their tips. This polarized growth requires the continuous delivery of secretory vesicles to the tip region. Vesicle delivery depends on Sec2p, the Guanine Exchange Factor (GEF) for the Rab GTPase Sec4p. GTP bound Sec4p is required for the transit of secre...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12799

    authors: Caballero-Lima D,Hautbergue GM,Wilson SA,Sudbery PE

    更新日期:2014-11-01 00:00:00

  • Cloning and characterization of the bundle-forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes.

    abstract::bfp, the structural gene of the major repeating bundle-forming pilus (BFP) subunit, was cloned from the enteroadherent factor (EAF) plasmid of enteropathogenic Escherichia coli (EPEC) strain B171 (O111:NM). The bfp open reading frame encoded a 193-amino-acid protein; comparison of this sequence with the biochemically ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb01147.x

    authors: Sohel I,Puente JL,Murray WJ,Vuopio-Varkila J,Schoolnik GK

    更新日期:1993-02-01 00:00:00

  • Comparative transcriptomics with a motility-deficient mutant leads to identification of a novel polysaccharide secretion system in Nostoc punctiforme.

    abstract::Many filamentous cyanobacteria are capable of gliding motility by an undefined mechanism. Within the heterocyst-forming clades, some strains, such as the Nostoc spp. and Fisherella spp., are motile only as specialized filaments termed hormogonia. Here we report on the phenotype of inactivation of a methyl-accepting ch...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12138

    authors: Risser DD,Meeks JC

    更新日期:2013-02-01 00:00:00

  • 'Form variation' of the O12 antigen is critical for persistence of Salmonella Typhimurium in the murine intestine.

    abstract::Salmonella enterica subspecies I serotypes are responsible for the vast majority of salmonellosis in mammals and birds, yet only a few factors specific to this group that allow them to persist in this niche have been identified. We show that STM0557, a S. enterica subspecies I-specific gene encoding an inner membrane ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06461.x

    authors: Bogomolnaya LM,Santiviago CA,Yang HJ,Baumler AJ,Andrews-Polymenis HL

    更新日期:2008-12-01 00:00:00

  • Hyperosmotic stress induces metacaspase- and mitochondria-dependent apoptosis in Saccharomyces cerevisiae.

    abstract::During the last years, several reports described an apoptosis-like programmed cell death process in yeast in response to different environmental aggressions. Here, evidence is presented that hyperosmotic stress caused by high glucose or sorbitol concentrations in culture medium induces in Saccharomyces cerevisiae a ce...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2005.04868.x

    authors: Silva RD,Sotoca R,Johansson B,Ludovico P,Sansonetty F,Silva MT,Peinado JM,Côrte-Real M

    更新日期:2005-11-01 00:00:00

  • A NapC/NirT-type cytochrome c (NrfH) is the mediator between the quinone pool and the cytochrome c nitrite reductase of Wolinella succinogenes.

    abstract::Wolinella succinogenes can grow by anaerobic respiration with nitrate or nitrite using formate as electron donor. Two forms of nitrite reductase were isolated from the membrane fraction of W. succinogenes. One form consisted of a 58 kDa polypeptide (NrfA) that was identical to the periplasmic nitrite reductase. The ot...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2000.01742.x

    authors: Simon J,Gross R,Einsle O,Kroneck PM,Kröger A,Klimmek O

    更新日期:2000-02-01 00:00:00

  • Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility.

    abstract::The c-di-GMP-binding effector protein FlgZ has been demonstrated to control motility in the opportunistic pathogen Pseudomonas aeruginosa and it was suggested that c-di-GMP-bound FlgZ impedes motility via its interaction with the MotCD stator. To further understand how motility is downregulated in P. aeruginosa and to...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14236

    authors: Bense S,Bruchmann S,Steffen A,Stradal TEB,Häussler S,Düvel J

    更新日期:2019-06-01 00:00:00

  • New erythromycin derivatives from Saccharopolyspora erythraea using sugar O-methyltransferases from the spinosyn biosynthetic gene cluster.

    abstract::Using a previously developed expression system based on the erythromycin-producing strain of Saccharopolyspora erythraea, O-methyltransferases from the spinosyn biosynthetic gene cluster of Saccharopolyspora spinosa have been shown to modify a rhamnosyl sugar attached to a 14-membered polyketide macrolactone. The spnI...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02594.x

    authors: Gaisser S,Lill R,Wirtz G,Grolle F,Staunton J,Leadlay PF

    更新日期:2001-09-01 00:00:00

  • Streptococcus mutans autolysin AtlA is a fibronectin-binding protein and contributes to bacterial survival in the bloodstream and virulence for infective endocarditis.

    abstract::Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2009.06903.x

    authors: Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JS

    更新日期:2009-11-01 00:00:00

  • Generalized approach to the regulation and integration of gene expression.

    abstract::The volume of electron flow through the cbb3 branch of the electron transport chain and the redox state of the quinone pool generate signals that regulate photosynthesis gene expression in Rhodobacter sphaeroides. An inhibitory signal is generated at the level of the catalytic subunit of the cbb3 cytochrome c oxidase ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.2001.02299.x

    authors: Oh JI,Kaplan S

    更新日期:2001-03-01 00:00:00