RloC: a wobble nucleotide-excising and zinc-responsive bacterial tRNase.

abstract：:Bacteria have obtained a variety of resistance mechanisms including toxin-antitoxin (TA) systems against bacteriophages (phages), whereas phages have also evolved to overcome bacterial anti-phage mechanisms. Dmd from T4 phage can suppress the toxicities of homologous toxins LsoA and RnlA from Escherichia coli, represe...

journal_title：Molecular microbiology

authors： Wan H,Otsuka Y,Gao ZQ,Wei Y,Chen Z,Masuda M,Yonesaki T,Zhang H,Dong YH

更新日期：2016-09-01 00:00:00

abstract：:Plasmodium vivax is one of four Plasmodium species that cause human malaria. P. vivax and a related simian malaria parasite, Plasmodium knowlesi, invade erythrocytes by binding the Duffy antigen/receptor for chemokines (DARC) through their respective Duffy binding proteins. Here we show that tyrosines 30 and 41 of DAR...

journal_title：Molecular microbiology

authors： Choe H,Moore MJ,Owens CM,Wright PL,Vasilieva N,Li W,Singh AP,Shakri R,Chitnis CE,Farzan M

更新日期：2005-03-01 00:00:00

abstract：:The involvement of signal transduction in the repair of radiation-induced damage to DNA has been known in eukaryotes but remains understudied in bacteria. This article for the first time demonstrates a role for the periplasmic lipoprotein (YfgL) with protein kinase activity transducing a signal for DNA strand break re...

journal_title：Molecular microbiology

authors： Khairnar NP,Kamble VA,Mangoli SH,Apte SK,Misra HS

更新日期：2007-07-01 00:00:00

abstract：:The suf and isc operons of Escherichia coli have been implicated in Fe-S cluster assembly. However, it has been unclear why E. coli has two systems for Fe-S cluster biosynthesis. We have examined the regulatory characteristics and mutant phenotypes of both operons to discern if the two operons have redundant functions...

journal_title：Molecular microbiology

authors： Outten FW,Djaman O,Storz G

更新日期：2004-05-01 00:00:00

abstract：:The nucleotide sequence of the ugp genes of Escherichia coli K-12, which encode a phosphate-limitation inducible uptake system for sn-glycerol-3-phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a p...

journal_title：Molecular microbiology

authors： Overduin P,Boos W,Tommassen J

更新日期：1988-11-01 00:00:00

abstract：:The guinea pig intestinal symbiont Metabacterium polyspora is an uncultured, endospore-forming member of the Firmicutes. Unlike most endospore-forming bacteria, sporulation is an obligate part of the M. polyspora life cycle when it is associated with a guinea pig. Binary fission is limited to a brief period in its lif...

journal_title：Molecular microbiology

authors： Ward RJ,Angert ER

更新日期：2008-03-01 00:00:00

abstract：:The serine-threonine kinase TOR, the Target of Rapamycin, is an important regulator of nutrient, energy and stress signaling in eukaryotes. Sch9, a Ser/Thr kinase of AGC family (the cAMP-dependent PKA, cGMP- dependent protein kinase G and phospholipid-dependent protein kinase C family), is a substrate of TOR. Here, we...

journal_title：Molecular microbiology

authors： Alves de Castro P,Dos Reis TF,Dolan SK,Oliveira Manfiolli A,Brown NA,Jones GW,Doyle S,Riaño-Pachón DM,Squina FM,Caldana C,Singh A,Del Poeta M,Hagiwara D,Silva-Rocha R,Goldman GH

更新日期：2016-11-01 00:00:00

abstract：:Escherichia coli cells use two distinct sensory circuits during chemotaxis towards carbohydrates. One circuit requires the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and is independent of any specific chemoreceptor, whereas the other uses a chemoreceptor-dependent sensory mechanism analogous to that...

journal_title：Molecular microbiology

authors： Kristich CJ,Glekas GD,Ordal GW

更新日期：2003-03-01 00:00:00

abstract：:Cells require a sensory system and a motility structure to achieve directed movement. Bacteria and archaea possess rotating filamentous motility structures that work in concert with the sensory chemotaxis system. This allows microorganisms to move along chemical gradients. The central response regulator protein CheY c...

journal_title：Molecular microbiology

authors： Li Z,Rodriguez-Franco M,Albers SV,Quax TEF

更新日期：2020-09-01 00:00:00

abstract：:Post-transcriptional repression of porin synthesis has emerged as a major function of Hfq-dependent, small non-coding RNAs (sRNAs). Many enterobacteria express OmpX-like porins, a family of outer membrane proteins whose physiological roles and structural properties have been studied intensively. While regulatory sRNAs...

journal_title：Molecular microbiology

authors： Papenfort K,Pfeiffer V,Lucchini S,Sonawane A,Hinton JC,Vogel J

更新日期：2008-05-01 00:00:00

abstract：:Evidence is presented that transcription of most of the early genes in the Streptomyces coelicolor A3(2) phage phi C31 is from a series of unusual promoters that depend on a function expressed early in the phi C31 lytic cycle. Primer extension analysis on the 5' ends of three early mRNAs, from samples prepared 10 min ...

journal_title：Molecular microbiology

authors： Ingham CJ,Crombie HJ,Bruton CJ,Chater KF,Hartley NM,Murphy GJ,Smith MC

更新日期：1993-09-01 00:00:00

abstract：:A key component to the success of Mycobacterium tuberculosis as a pathogen is the ability to sense and adapt metabolically to the diverse range of conditions encountered in vivo, such as oxygen tension, environmental pH and nutrient availability. Although nitrogen is an essential nutrient for every organism, little is...

journal_title：Molecular microbiology

authors： Williams KJ,Jenkins VA,Barton GR,Bryant WA,Krishnan N,Robertson BD

更新日期：2015-09-01 00:00:00

abstract：:Crystallographic structure refinement at very high resolutions of a dozen periplasmic receptors has revealed that, though they have different sizes (26 to 60 kDa) and little sequence homology, they have high tertiary structure similarity. They consist of two distinct globular domains bisected by a cleft or groove wher...

journal_title：Molecular microbiology

authors： Quiocho FA,Ledvina PS

更新日期：1996-04-01 00:00:00

abstract：:Protein quality control and proteolysis are involved in cell maintenance and environmental acclimatization in bacteria and eukaryotes. The AAA protease FtsH2 of the cyanobacterium Synechocystis sp. PCC 6803 was identified during a screening for mutants impaired in osmoregulation. The ftsH2(-) mutant was salt sensitive...

journal_title：Molecular microbiology

authors： Stirnberg M,Fulda S,Huckauf J,Hagemann M,Krämer R,Marin K

更新日期：2007-01-01 00:00:00

abstract：:Candida albicans hyphae grow in a highly polarized fashion from their tips. This polarized growth requires the continuous delivery of secretory vesicles to the tip region. Vesicle delivery depends on Sec2p, the Guanine Exchange Factor (GEF) for the Rab GTPase Sec4p. GTP bound Sec4p is required for the transit of secre...

journal_title：Molecular microbiology

authors： Caballero-Lima D,Hautbergue GM,Wilson SA,Sudbery PE

更新日期：2014-11-01 00:00:00

abstract：:5'-nucleotidases (5'-NTs) are enzymes that catalyze the hydrolysis of nucleoside monophosphates to produce nucleosides and phosphate. Since the identification of adenosine synthase A (AdsA) in Staphylococcus aureus in 2009, several other 5'-NTs have been discovered in Gram-positive cocci, mainly in streptococci. Despi...

journal_title：Molecular microbiology

authors： Soh KY,Loh JMS,Proft T

更新日期：2020-04-01 00:00:00

abstract：:RNA secondary structure is important in a wide variety of biological processes, but relatively little is known about the pathways and kinetics of RNA folding. When the IS10 transposase (tnp) gene is transcribed from a promoter outside the element, little increase in tnp expression is observed. This protection from out...

journal_title：Molecular microbiology

authors： Ma CK,Kolesnikow T,Rayner JC,Simons EL,Yim H,Simons RW

更新日期：1994-12-01 00:00:00

abstract：:Eukaryotic cells have evolved a complex set of intracellular organelles, each of which possesses a specific complement of enzymes and performs unique metabolic functions. This compartmentalization of cellular functions provides a level of metabolic control not available to prokaryotes. However, it presents the eukaryo...

journal_title：Molecular microbiology

authors： Aitchison JD,Nuttley WM,Szilard RK,Brade AM,Glover JR,Rachubinski RA

更新日期：1992-12-01 00:00:00

abstract：:We have shown previously that deletion of the Saccharomyces cerevisiae UMP1 gene encoding the 20S proteasome maturase causes sensitivity to UV radiation. In the current report, we have extended this finding to show that mutations specifically compromising chymotrypsin-like or trypsin-like activity of 20S proteasome pe...

journal_title：Molecular microbiology

authors： Podlaska A,McIntyre J,Skoneczna A,Sledziewska-Gojska E

更新日期：2003-09-01 00:00:00

abstract：:We have isolated RNA polymerase from Mycobacterium smegmatis and established conditions for specific transcription initiation in vitro. The M. smegmatis enzyme has a strong dependence on supercoiling of the DNA substrate for transcription from mycobacterial promoters. We also show that RNA polymerase is the target for...

journal_title：Molecular microbiology

authors： Levin ME,Hatfull GF

更新日期：1993-04-01 00:00:00

abstract：:The essential proteins DnaB, DnaD and DnaI of Bacillus subtilis are required for initiation, but not elongation, of DNA replication, and for replication restart at stalled forks. The interactions and functions of these proteins have largely been determined in vitro based on their roles in replication restart. During r...

journal_title：Molecular microbiology

authors： Smits WK,Goranov AI,Grossman AD

更新日期：2010-01-01 00:00:00

abstract：:The role of cAMP signalling during germination of asexual spores (conidia) of the filamentous fungus Aspergillus nidulans was investigated. A. nidulans strains defective for adenylate cyclase (CyaA) or for the functionally overlapping cAMP-dependent protein kinase (PkaA) and newly characterized SchA protein kinase, ho...

journal_title：Molecular microbiology

authors： Fillinger S,Chaveroche MK,Shimizu K,Keller N,d'Enfert C

更新日期：2002-05-01 00:00:00

abstract：:ICEMlSym(R7A) of Mesorhizobium loti is an integrative and conjugative element (ICE) that confers the ability to form a nitrogen-fixing symbiosis with Lotus species. Horizontal transfer is activated by TraR and N-acyl-homoserine lactone (AHL), which can stimulate ICE excision in 100% of cells. However, in wild-type cu...

journal_title：Molecular microbiology

authors： Ramsay JP,Major AS,Komarovsky VM,Sullivan JT,Dy RL,Hynes MF,Salmond GP,Ronson CW

更新日期：2013-01-01 00:00:00

abstract：:Biofilms are communities of microbial cells that are encased in a self-produced, polymeric matrix and are adherent to a surface. For several species of bacteria, an enhanced ability to form biofilms has been linked with an increased capability to produce exopolymers. To identify exopolymers of Bacillus subtilis that c...

journal_title：Molecular microbiology

authors： Stanley NR,Lazazzera BA

更新日期：2005-08-01 00:00:00

abstract：:Escherichia coli has two heat shock regulons under the transcriptional control of Esigma32 and EsigmaE RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that...

journal_title：Molecular microbiology

authors： Klein G,Dartigalongue C,Raina S

更新日期：2003-04-01 00:00:00

abstract：:Ustilago maydis, the causal agent of corn smut disease, displays dimorphic growth in which it alternates between a budding haploid saprophyte and a filamentous dikaryotic pathogen. We are interested in identifying the genetic determinants of filamentous growth and pathogenicity in U. maydis. To do this, we have taken ...

journal_title：Molecular microbiology

authors： Mayorga ME,Gold SE

更新日期：1999-11-01 00:00:00

abstract：:Fragments of the gene encoding the major outer membrane porin protein (MOMP) of Chlamydia trachomatis serovar L1 were ligated into the pUC plasmid vectors to give a series of overlapping recombinants expressing MOMP from the lac promoter. Induction of this promoter with IPTG leads to high-level expression of the recom...

journal_title：Molecular microbiology

authors： Pickett MA,Ward ME,Clarke IN

更新日期：1988-09-01 00:00:00

abstract：:Red cell invasion by Plasmodium merozoites involves multiple steps such as attachment, apical reorientation, junction formation and entry into a parasitophorous vacuole. These steps are mediated by specific molecular interactions. P. vivax and the simian parasite P. knowlesi require interaction with the Duffy blood gr...

journal_title：Molecular microbiology

authors： Singh AP,Ozwara H,Kocken CH,Puri SK,Thomas AW,Chitnis CE

更新日期：2005-03-01 00:00:00

abstract：:In bacteria, DNA supercoil movement is restricted to subchromosomal regions or 'domains.' To elucidate the nature of domain boundaries, we analysed reaction kinetics for gammadelta site-specific resolution in six chromosomal intervals ranging in size from 14 to 90 kb. In stationary cultures of Salmonella typhimurium, ...

journal_title：Molecular microbiology

authors： Staczek P,Higgins NP

更新日期：1998-09-01 00:00:00

abstract：:Gene expression in fungi by ambient pH is regulated via a conserved signalling cascade whose terminal component is the zinc finger transcription factor PacC/Rim1p. We have identified a pacC orthologue in the vascular wilt pathogen Fusarium oxysporum that binds the consensus 5'-GCCAAG-3' sequence and is proteolytically...

journal_title：Molecular microbiology

authors： Caracuel Z,Roncero MI,Espeso EA,González-Verdejo CI,García-Maceira FI,Di Pietro A

更新日期：2003-05-01 00:00:00