Cdv-based cell division and cell cycle organization in the thaumarchaeon Nitrosopumilus maritimus.


:Cell division is mediated by different mechanisms in different evolutionary lineages. While bacteria and euryarchaea utilize an FtsZ-based mechanism, most crenarchaea divide using the Cdv system, related to the eukaryotic ESCRT-III machinery. Intriguingly, thaumarchaeal genomes encode both FtsZ and Cdv protein homologues, raising the question of their division mode. Here, we provide evidence indicating that Cdv is the primary division system in the thaumarchaeon Nitrosopumilus maritimus. We also show that the cell cycle is differently organized as compared to hyperthermophilic crenarchaea, with a longer pre-replication phase and a shorter post-replication stage. In particular, the time required for chromosome replication is remarkably extensive, 15-18 h, indicating a low replication rate. Further, replication did not continue to termination in a significant fraction of N. maritimus cell populations following substrate depletion. Both the low replication speed and the propensity for replication arrest are likely to represent adaptations to extremely oligotrophic environments. The results demonstrate that thaumarchaea, crenarchaea and euryarchaea display differences not only regarding phylogenetic affiliations and gene content, but also in fundamental cellular and physiological characteristics. The findings also have implications for evolutionary issues concerning the last archaeal common ancestor and the relationship between archaea and eukaryotes.


Mol Microbiol


Molecular microbiology


Pelve EA,Lindås AC,Martens-Habbena W,de la Torre JR,Stahl DA,Bernander R




Has Abstract


2011-11-01 00:00:00












  • CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.

    abstract::Stimulation of Escherichia coli with acetate elevates the acetylation level of the chemotaxis response regulator CheY. This elevation, in an unknown mechanism, activates CheY to generate clockwise rotation. Here, using quantitative selective reaction monitoring mass spectrometry and high-resolution targeted mass spect...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Fraiberg M,Afanzar O,Cassidy CK,Gabashvili A,Schulten K,Levin Y,Eisenbach M

    更新日期:2015-01-01 00:00:00

  • Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.

    abstract::Computer analysis of the crystallographic structure of the A subunit of Escherichia coli heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully ass...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pizza M,Domenighini M,Hol W,Giannelli V,Fontana MR,Giuliani MM,Magagnoli C,Peppoloni S,Manetti R,Rappuoli R

    更新日期:1994-10-01 00:00:00

  • Expression of the Fis protein is sustained in late-exponential- and stationary-phase cultures of Salmonella enterica serovar Typhimurium grown in the absence of aeration.

    abstract::The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is almost undetectable at the end of the exponential phase. We discovered that this patt...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: O Cróinín T,Dorman CJ

    更新日期:2007-10-01 00:00:00

  • The cell biology of secondary endosymbiosis--how parasites build, divide and segregate the apicoplast.

    abstract::Protozoan parasites of the phylum Apicomplexa harbour a chloroplast-like organelle, the apicoplast. The biosynthetic pathways localized to this organelle are of cyanobacterial origin and therefore offer attractive targets for the development of new drugs for the treatment of malaria and toxoplasmosis. The apicoplast a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Vaishnava S,Striepen B

    更新日期:2006-09-01 00:00:00

  • Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.

    abstract::Natural competence in Streptococcus pneumoniae is regulated by a quorum-sensing mechanism consisting of a competence-stimulating peptide (CSP), its dedicated secretion apparatus (ComAB), its histidine kinase receptor (ComD) and a response regulator (ComE). In this report, we show that ComE is a DNA-binding protein tha...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ween O,Gaustad P,Håvarstein LS

    更新日期:1999-08-01 00:00:00

  • The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction.

    abstract::The recently identified recA gene of the naturally transformable bacterium Streptococcus pneumoniae has been further characterized by constructing a recA null mutation and by investigating its regulation. The recA mutation has been shown to confer both DNA repair (as judged from sensitivity to u.v. and methyl methane ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Martin B,García P,Castanié MP,Claverys JP

    更新日期:1995-01-01 00:00:00

  • Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.

    abstract::The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in certain bean cultivars. Previous studies have shown that when PPHGI-1 ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Neale HC,Jackson RW,Preston GM,Arnold DL

    更新日期:2018-11-01 00:00:00

  • Agr-independent regulation of fibronectin-binding protein(s) by the regulatory locus sar in Staphylococcus aureus.

    abstract::Fibronectin-binding proteins (FnBPs) are thought to be important for the attachment of Staphylococcus aureus during infection. The regulation of the genes fnbA and fnbB by the global regulatory loci sar and agr was examined using site-specific regulatory mutants of S. aureus strain Newman. The results from binding ass...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wolz C,Pöhlmann-Dietze P,Steinhuber A,Chien YT,Manna A,van Wamel W,Cheung A

    更新日期:2000-04-01 00:00:00

  • Analysis of a cloned sequence of Legionella pneumophila encoding a 38 kD metalloprotease possessing haemolytic and cytotoxic activities.

    abstract::The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Quinn FD,Tompkins LS

    更新日期:1989-06-01 00:00:00

  • Cell envelope components contributing to biofilm growth and survival of Pseudomonas putida in low-water-content habitats.

    abstract::Bacteria in terrestrial habitats frequently reside as biofilm communities on surfaces that are unsaturated, i.e. biofilms are covered in water films varying in thickness depending on the environmental conditions. Water availability in these habitats is influenced by the osmolarity of the water (solute stress) and by c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: van de Mortel M,Halverson LJ

    更新日期:2004-05-01 00:00:00

  • A nonameric core sequence is required upstream of the LYS genes of Saccharomyces cerevisiae for Lys14p-mediated activation and apparent repression by lysine.

    abstract::The expression of the structural genes for lysine (LYS) biosynthesis is controlled by a pathway-specific regulation mediated by the transcriptional activator Lys14 in the presence of alpha-aminoadipate semialdehyde, an intermediate of the pathway acting as a co-inducer. Owing to end product inhibition of the first ste...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Becker B,Feller A,el Alami M,Dubois E,Piérard A

    更新日期:1998-07-01 00:00:00

  • Suppression of temperature-sensitive assembly mutants of heat-labile enterotoxin B subunits.

    abstract::Deletions or substitutions of amino acids at the carboxyl-terminus of the heat-labile enterotoxin B subunit (EtxB) affect its assembly into pentamers in a temperature-dependent manner. At 42 degrees C, the mutations prevent the B subunits from achieving their final pentameric structure resulting in membrane associatio...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sandkvist M,Bagdasarian M

    更新日期:1993-11-01 00:00:00

  • In vitro characterization of constitutive CysB proteins from Salmonella typhimurium.

    abstract::Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Colyer TE,Kredich NM

    更新日期:1996-07-01 00:00:00

  • Repair of cyclobutane pyrimidine dimers and 6-4 photoproducts in the fission yeast Schizosaccharomyces pombe.

    abstract::We have measured repair of both of the major lesions induced by ultraviolet irradiation (cyclobutane pyrimidine dimers and 6-4 photoproducts) in wild-type Schizosaccharomyces pombe and in selected rad mutants, including mutants with deletions in genes from the main phenotypic groups. We find that rad13 delta, rad15 an...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: McCready S,Carr AM,Lehmann AR

    更新日期:1993-11-01 00:00:00

  • Incorporation of a polypeptide segment into the beta-domain pore during the assembly of a bacterial autotransporter.

    abstract::Bacterial autotransporters consist of an N-terminal 'passenger domain' that is transported into the extracellular space by an unknown mechanism and a C-terminal 'beta-domain' that forms a beta-barrel in the outer membrane. Recent studies have revealed that fully assembled autotransporters have an unusual architecture ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ieva R,Skillman KM,Bernstein HD

    更新日期:2008-01-01 00:00:00

  • Post-transcriptional regulation by distal Shine-Dalgarno sequences in the grpE-dnaK intergenic region of Streptococcus mutans.

    abstract::A unique 373 bp region (igr66) between grpE and dnaK of Streptococcus mutans lacks a promoter but is required for optimal production of DnaK. Northern blotting using probes specific to hrcA, igr66 or dnaK revealed multiple transcripts produced from the dnaK operon and 5'-RACE mapped 5' termini of multiple dnaK transcr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Palmer SR,Burne RA

    更新日期:2015-10-01 00:00:00

  • The permease gene nagE2 is the key to N-acetylglucosamine sensing and utilization in Streptomyces coelicolor and is subject to multi-level control.

    abstract::The availability of nutrients is a major determinant for the timing of morphogenesis and antibiotic production in the soil-dwelling bacterium Streptomyces coelicolor. Here we show that N-acetylglucosamine transport, the first step of an important nutrient signalling cascade, is mediated by the NagE2 permease of the ph...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nothaft H,Rigali S,Boomsma B,Swiatek M,McDowall KJ,van Wezel GP,Titgemeyer F

    更新日期:2010-03-01 00:00:00

  • Involvement of multiple distinct Bordetella receptor proteins in the utilization of iron liberated from transferrin by host catecholamine stress hormones.

    abstract::Bordetella bronchiseptica is a pathogen that can acquire iron using its native alcaligin siderophore system, but can also use the catechol xenosiderophore enterobactin via the BfeA outer membrane receptor. Transcription of bfeA is positively controlled by a regulator that requires induction by enterobactin. Catecholam...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Armstrong SK,Brickman TJ,Suhadolc RJ

    更新日期:2012-05-01 00:00:00

  • yst gene expression in Yersinia enterocolitica is positively regulated by a chromosomal region that is highly homologous to Escherichia coli host factor 1 gene (hfq).

    abstract::Yersinia enterocolitica produces heat-stable enterotoxin (Y-ST) as one of its virulence factors. The yst gene, however, frequently and spontaneously becomes inactive (silent) during storage, which is accompanied by concurrent changes in some biological properties such as colony morphology, growth rate, carbon fermenta...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nakao H,Watanabe H,Nakayama S,Takeda T

    更新日期:1995-12-01 00:00:00

  • FliL is essential for swarming: motor rotation in absence of FliL fractures the flagellar rod in swarmer cells of Salmonella enterica.

    abstract::fliL is the first gene in a flagellar operon that specifies members of the switch complex and type III export system in Salmonella enterica and Escherichia coli, but no function has been ascribed to this gene thus far. Here we report that a fliL mutant is slightly impaired for swimming but completely defective in swar...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Attmannspacher U,Scharf BE,Harshey RM

    更新日期:2008-04-01 00:00:00

  • The diverse family of MmpL transporters in mycobacteria: from regulation to antimicrobial developments.

    abstract::Mycobacterial genomes contain large sets of loci encoding membrane proteins that belong to a family of multidrug resistance pumps designated Resistance-Nodulation-Cell Division (RND) permeases. Mycobacterial membrane protein Large (MmpL) transporters represent a subclass of RND transporters known to participate in the...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Viljoen A,Dubois V,Girard-Misguich F,Blaise M,Herrmann JL,Kremer L

    更新日期:2017-06-01 00:00:00

  • Contribution of N- and C-terminal domains to the function of Hsp90 in Saccharomyces cerevisiae.

    abstract::The molecular chaperone Hsp90 is a regulatory component of some key signalling proteins in the cytosol of eukaryotic cells. For some of these functions, its interaction with co-chaperones is required. Limited proteolysis defined stable folded units of Hsp90. Both an N-terminal (N210) and a C-terminal (262C) fragment i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Scheibel T,Weikl T,Rimerman R,Smith D,Lindquist S,Buchner J

    更新日期:1999-11-01 00:00:00

  • KOPS-guided DNA translocation by FtsK safeguards Escherichia coli chromosome segregation.

    abstract::The septum-located DNA translocase, FtsK, acts to co-ordinate the late steps of Escherichia coli chromosome segregation with cell division. The FtsK gamma regulatory subdomain interacts with 8 bp KOPS DNA sequences, which are oriented from the replication origin to the terminus region (ter) in each arm of the chromoso...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sivanathan V,Emerson JE,Pages C,Cornet F,Sherratt DJ,Arciszewska LK

    更新日期:2009-02-01 00:00:00

  • Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hinnebusch J,Bergström S,Barbour AG

    更新日期:1990-05-01 00:00:00

  • Inversion within the haloalkaliphilic virus phi Ch1 DNA results in differential expression of structural proteins.

    abstract::The sequence of phi Ch1 contains an open reading frame (int1) in the central part of its genome that belongs to the lambda integrase family of site-specific recombinases. Sequence similarities to known integrases include the highly conserved tetrad R-H-R-Y. The flanking sequences of int1 contain several direct repeats...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rössler N,Klein R,Scholz H,Witte A

    更新日期:2004-04-01 00:00:00

  • High-level, constitutive expression of the mgtC gene confers increased thermotolerance on Salmonella enterica serovar Typhimurium.

    abstract::We found that mutations that increased the transcription of the mgtCBR (Mg2+ transport-related) operon conferred increased thermotolerance on this organism. The 5' leader of the mgtCBR mRNA contains two short open reading frames (ORFs), mgtM and mgtP, whose translation regulates the expression of the mgtCBR operon by ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gall AR,Hegarty AE,Datsenko KA,Westerman RP,SanMiguel P,Csonka LN

    更新日期:2018-08-01 00:00:00

  • Control of FimY translation and type 1 fimbrial production by the arginine tRNA encoded by fimU in Salmonella enterica serovar Typhimurium.

    abstract::Expression of type 1 fimbriae in Salmonella enterica serovar Typhimurium undergoes phase variation or alteration between a fimbriate and a non-fimbriate phenotype. This variation is known to be dependent upon environmental conditions in vitro and is thought to be a complex process involving regulation by a number of p...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tinker JK,Clegg S

    更新日期:2001-05-01 00:00:00

  • DNA sequence analysis of three missense mutations affecting colicin E3 bactericidal activity.

    abstract::We have determined the nucleotide sequence changes caused by three missense mutations leading to the production of inactive colicin E3 proteins. The ceaC1 mutation, affecting the translocation of colicin E3 through bacterial membranes, is caused by a serine to phenylalanine change at position 37 within the glycine-ric...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Escuyer V,Mock M

    更新日期:1987-07-01 00:00:00

  • Lipid body formation plays a central role in cell fate determination during developmental differentiation of Myxococcus xanthus.

    abstract::Cell differentiation is widespread during the development of multicellular organisms, but rarely observed in prokaryotes. One example of prokaryotic differentiation is the gram-negative bacterium Myxococcus xanthus. In response to starvation, this gliding bacterium initiates a complex developmental programme that resu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hoiczyk E,Ring MW,McHugh CA,Schwär G,Bode E,Krug D,Altmeyer MO,Lu JZ,Bode HB

    更新日期:2009-10-01 00:00:00

  • Uropathogenic Escherichia coli use d-serine deaminase to modulate infection of the murine urinary tract.

    abstract::Although once thought to be unique to bacteria, d-amino acids are also produced by mammals. For example, d-serine is excreted in human urine at concentrations ranging from 3.0 to 40 micro g ml-1. An epidemiological survey demonstrated that urine isolates of E. coli are more likely to catabolise d-serine via expression...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Roesch PL,Redford P,Batchelet S,Moritz RL,Pellett S,Haugen BJ,Blattner FR,Welch RA

    更新日期:2003-07-01 00:00:00