Polynucleotide phosphorylase is required for the rapid degradation of the RNase E-processed rpsO mRNA of Escherichia coli devoid of its 3' hairpin.

abstract：:Bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. Nucleotide sequences from the locus involved in coordinate regulation of B. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal DNA from virulent (Vir+) and avirulent (Vir-) strains of ...

journal_title：Molecular microbiology

authors： Monack DM,Arico B,Rappuoli R,Falkow S

更新日期：1989-12-01 00:00:00

abstract：:In this study we show for the first time the intracellular distribution of a K39 kinesin homologue in Leishmania donovani, a medically important parasite of humans. Further, we demonstrated that this motor protein is expressed in both the insect and mammalian developmental forms (i.e. promastigote and amastigotes) of ...

journal_title：Molecular microbiology

authors： Gerald NJ,Coppens I,Dwyer DM

更新日期：2007-02-01 00:00:00

abstract：:Fungi appear to be unique in their requirement for a third soluble translation elongation factor. This factor, designated elongation factor 3 (EF-3), was first described in the yeast Saccharomyces cerevisiae and has subsequently been identified in a wide range of fungal species including Candida albicans and Schizosac...

journal_title：Molecular microbiology

authors： Belfield GP,Tuite MF

更新日期：1993-08-01 00:00:00

abstract：:To determine if the food-grade bacterium Lactococcus lactis holds promise as a vaccine antigen delivery vector we have investigated whether this bacterium can be made to produce high levels of a heterologous protein antigen. A regulated expression system has been developed which may be generally suitable for the expre...

journal_title：Molecular microbiology

authors： Wells JM,Wilson PW,Norton PM,Gasson MJ,Le Page RW

更新日期：1993-06-01 00:00:00

abstract：:Borrelia burgdorferi survives in an enzootic cycle, and Dps proteins protect DNA against damage during starvation or oxidative stress. The role of a Dps homologue encoded by Borrelia in spirochaete survival was assessed. Dps-deficient spirochaetes were infectious in mice via needle-inoculation at the dose of 10(5) spi...

journal_title：Molecular microbiology

authors： Li X,Pal U,Ramamoorthi N,Liu X,Desrosiers DC,Eggers CH,Anderson JF,Radolf JD,Fikrig E

更新日期：2007-02-01 00:00:00

abstract：:Saccharomyces cerevisiae neutral trehalase, encoded by NTH1, controls trehalose hydrolysis in response to multiple stress conditions, including nutrient limitation. The presence of three stress responsive elements (STREs, CCCCT) in the NTH1 promoter suggested that the transcriptional activator proteins Msn2 and Msn4, ...

journal_title：Molecular microbiology

authors： Zähringer H,Thevelein JM,Nwaka S

更新日期：2000-01-01 00:00:00

abstract：:Mechanisms of hydrogen peroxide generation in Escherichia coli were investigated using a strain lacking scavenging enzymes. Surprisingly, the deletion of many abundant flavoenzymes that are known to autoxidize in vitro did not substantially lessen overall H(2)O(2) formation. However, H(2)O(2) production diminished by ...

journal_title：Molecular microbiology

authors： Korshunov S,Imlay JA

更新日期：2010-03-01 00:00:00

abstract：:Crown gall tumorigenesis by Agrobacterium tumefaciens requires the co-ordinate transcriptional induction of a set of pathogenesis genes. At least three classes of environmental stimuli act synergistically to induce these genes: (i) monocyclic aromatic hydrocarbons such as acetosyringone, coniferyl alcohol, and vanilli...

journal_title：Molecular microbiology

authors： Winans SC

更新日期：1991-10-01 00:00:00

abstract：:We have measured repair of both of the major lesions induced by ultraviolet irradiation (cyclobutane pyrimidine dimers and 6-4 photoproducts) in wild-type Schizosaccharomyces pombe and in selected rad mutants, including mutants with deletions in genes from the main phenotypic groups. We find that rad13 delta, rad15 an...

journal_title：Molecular microbiology

authors： McCready S,Carr AM,Lehmann AR

更新日期：1993-11-01 00:00:00

abstract：:The nucleotide sequence of the proximal half of the rfb region of Shigella flexneri has been determined, and the genes encoding enzymes involved in the biosynthesis of dTDP-rhamnose have been identified. These genes show strong homology to the rfb genes encoding dTDP-rhamnose biosynthesis in Salmonella enterica serova...

journal_title：Molecular microbiology

authors： Macpherson DF,Manning PA,Morona R

更新日期：1994-01-01 00:00:00

abstract：:Haemophilus influenzae is a human-specific pathogen and a major source of morbidity worldwide. Infection with this organism begins with colonization of the nasopharynx, a process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein is the major adhesin ex-pressed by a subset of...

journal_title：Molecular microbiology

authors： Laarmann S,Cutter D,Juehne T,Barenkamp SJ,St Geme JW

更新日期：2002-11-01 00:00:00

abstract：:The haemolysin of Haemophilus ducreyi is the newest member of the Proteus/Serratia family of pore-forming toxins. In order to assess the role of the haemolysin in virulence, we constructed an isogenic haemolysin-deficient mutant of H. ducreyi strain 35000 This strain, designated 35000-3, lacks detectable haemolytic ac...

journal_title：Molecular microbiology

authors： Palmer KL,Goldman WE,Munson RS Jr

更新日期：1996-07-01 00:00:00

abstract：:The nucleotide sequence of the mip genes and their inferred amino acid sequences were determined from 35 Legionella species and compared with the published sequences for L. pneumophila, L. micdadei and L. longbeachae. The sequences were 69-97% conserved at the nucleotide level and 82-99% at the amino acid level, with ...

journal_title：Molecular microbiology

authors： Ratcliff RM,Donnellan SC,Lanser JA,Manning PA,Heuzenroeder MW

更新日期：1997-09-01 00:00:00

abstract：:XylR is a σ⁵⁴-dependent transcriptional factor of Pseudomonas putida that activates the Pu promoter of the TOL plasmid upon binding its natural effector, m-xylene. The search for mutants of the signal-sensing module of XylR that respond to the xenobiotic compound 2,4-dinitrotoluene recurrently yields protein variants ...

journal_title：Molecular microbiology

authors： de Las Heras A,de Lorenzo V

更新日期：2011-03-01 00:00:00

abstract：:Atovaquone represents a class of antimicrobial agents with a broad-spectrum activity against various parasitic infections, including malaria, toxoplasmosis and Pneumocystis pneumonia. In malaria parasites, atovaquone inhibits mitochondrial electron transport at the level of the cytochrome bc1 complex and collapses mit...

journal_title：Molecular microbiology

authors： Srivastava IK,Morrisey JM,Darrouzet E,Daldal F,Vaidya AB

更新日期：1999-08-01 00:00:00

abstract：:The cheA gene encodes two overlapping polypeptides with a common carboxyl terminus: CheAL and CheAS. CheAL plays a central role in the Escherichia coli chemotaxis signalling pathway by autophosphorylation and transferring the phosphate to both CheY and CheB. On the other hand, the physiological functions of CheAS rema...

journal_title：Molecular microbiology

authors： Wang H,Matsumura P

更新日期：1996-02-01 00:00:00

abstract：:Biofilms are communities of microbial cells that are encased in a self-produced, polymeric matrix and are adherent to a surface. For several species of bacteria, an enhanced ability to form biofilms has been linked with an increased capability to produce exopolymers. To identify exopolymers of Bacillus subtilis that c...

journal_title：Molecular microbiology

authors： Stanley NR,Lazazzera BA

更新日期：2005-08-01 00:00:00

abstract：:Cell-cell communication via the production and detection of chemical signal molecules has been the focus of a great deal of research over the past decade. One class of chemical signals widely used by proteobacteria consists of N-acyl-homoserine lactones, which are synthesized by proteins related to LuxI of Vibrio fisc...

journal_title：Molecular microbiology

authors： Pappas KM,Weingart CL,Winans SC

更新日期：2004-08-01 00:00:00

abstract：:Deletions or substitutions of amino acids at the carboxyl-terminus of the heat-labile enterotoxin B subunit (EtxB) affect its assembly into pentamers in a temperature-dependent manner. At 42 degrees C, the mutations prevent the B subunits from achieving their final pentameric structure resulting in membrane associatio...

journal_title：Molecular microbiology

authors： Sandkvist M,Bagdasarian M

更新日期：1993-11-01 00:00:00

abstract：:The Escherichia coli DNA glycosylase Mug excises 3,N(4)-ethenocytosines (epsilon C) and uracils from DNA, but its biological function is obscure. This is because epsilon C is not found in E. coli DNA, and uracil-DNA glycosylase (Ung), a distinct enzyme, is much more efficient at removing uracils from DNA than Mug. We ...

journal_title：Molecular microbiology

authors： Mokkapati SK,Fernández de Henestrosa AR,Bhagwat AS

更新日期：2001-09-01 00:00:00

abstract：:The stable maintenance of low-copy-number plasmids requires active partitioning, with the most common mechanism in prokaryotes involving the ATPase ParA. ParA proteins undergo intricate spatiotemporal relocations across the nucleoid, dynamics that function to position plasmids at equally spaced intervals. This spacing...

journal_title：Molecular microbiology

authors： Howard M,Gerdes K

更新日期：2010-10-01 00:00:00

abstract：:The gene cbhA from the cellulolytic bacterium Cellulomonas fimi encodes a protein of 872 amino acids designated cellobiohydrolase A (CbhA). Mature CbhA contains 832 amino acid residues and has a predicted molecular mass of 85,349 Da. It is composed of five domains: an N-terminal catalytic domain, three repeated sequen...

journal_title：Molecular microbiology

authors： Meinke A,Gilkes NR,Kwan E,Kilburn DG,Warren RA,Miller RC Jr

更新日期：1994-05-01 00:00:00

abstract：:Fibronectin-binding proteins (FnBPs) are thought to be important for the attachment of Staphylococcus aureus during infection. The regulation of the genes fnbA and fnbB by the global regulatory loci sar and agr was examined using site-specific regulatory mutants of S. aureus strain Newman. The results from binding ass...

journal_title：Molecular microbiology

authors： Wolz C,Pöhlmann-Dietze P,Steinhuber A,Chien YT,Manna A,van Wamel W,Cheung A

更新日期：2000-04-01 00:00:00

abstract：:The bacterium Streptomyces davaonensis produces the antibiotic roseoflavin, which is a riboflavin (vitamin B2 ) analog. The key enzyme of roseoflavin biosynthesis is the 8-demethyl-8-amino-riboflavin-5'-phosphate (AFP) synthase RosB which synthesizes AFP from riboflavin-5'-phosphate. AFP is not a substrate for the las...

journal_title：Molecular microbiology

authors： Schneider C,Konjik V,Kißling L,Mack M

更新日期：2020-10-01 00:00:00

abstract：:mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E. coli and exami...

journal_title：Molecular microbiology

authors： Moe PC,Blount P,Kung C

更新日期：1998-05-01 00:00:00

abstract：:Bacterial cytokinesis requires the divisome, a complex of proteins that co-ordinates the invagination of the cytoplasmic membrane, inward growth of the peptidoglycan layer and the outer membrane. Assembly of the cell division proteins is tightly regulated and the order of appearance at the future division site is well...

journal_title：Molecular microbiology

authors： van den Ent F,Vinkenvleugel TM,Ind A,West P,Veprintsev D,Nanninga N,den Blaauwen T,Löwe J

更新日期：2008-04-01 00:00:00

abstract：:The Escherichia coli rodA and ftsW genes and the spoVE gene of Bacillus subtilis encode membrane proteins that control peptidoglycan synthesis during cellular elongation, division and sporulation respectively. While rodA and ftsW are essential genes in E. coli, the B. subtilis spoVE gene is dispensable for growth and ...

journal_title：Molecular microbiology

authors： Henriques AO,Glaser P,Piggot PJ,Moran CP Jr

更新日期：1998-04-01 00:00:00

abstract：:Penicillium marneffei is a thermally dimorphic opportunistic human pathogen with a saprophytic filamentous hyphal form at 25 degrees C and a pathogenic unicellular yeast form at 37 degrees C. During infection. P. marneffei yeast cells exist intracellularly in macrophages. To cope with nutrient deprivation during the i...

journal_title：Molecular microbiology

authors： Cánovas D,Andrianopoulos A

更新日期：2006-12-01 00:00:00

abstract：:The HPr kinase of Gram-positive bacteria is an ATP-dependent serine protein kinase, which phosphorylates the HPr protein of the bacterial phosphotransferase system (PTS) and is involved in the regulation of carbohydrate metabolism. The hprK gene from Enterococcus faecalis was cloned via polymerase chain reaction (PCR)...

journal_title：Molecular microbiology

authors： Kravanja M,Engelmann R,Dossonnet V,Blüggel M,Meyer HE,Frank R,Galinier A,Deutscher J,Schnell N,Hengstenberg W

更新日期：1999-01-01 00:00:00

abstract：:We describe a chemotactic-like response of Escherichia coli strains lacking most of the known chemotaxis machinery but containing high levels of the response regulator CheY. The bacteria accumulated in aspartate-containing capillaries, they formed rings on tryptone-containing semisolid agar, and the probability of cou...

journal_title：Molecular microbiology

authors： Barak R,Eisenbach M

更新日期：1999-02-01 00:00:00