Translation elongation factor 3: a fungus-specific translation factor?


:Fungi appear to be unique in their requirement for a third soluble translation elongation factor. This factor, designated elongation factor 3 (EF-3), was first described in the yeast Saccharomyces cerevisiae and has subsequently been identified in a wide range of fungal species including Candida albicans and Schizosaccharomyces pombe. EF-3 exhibits ribosome-dependent ATPase and GTPase activities that are not intrinsic to the fungal ribosome, but which are essential for translation elongation. Recent studies on the structure of EF-3 from several fungal species have shown that it consists of a repeated domain, with each domain containing the expected putative ATP- and GTP-binding motifs. Overall, EF-3 shows striking amino acid similarity to members of the ATP-binding Cassette (ABC) family of membrane-associated transport proteins although EF-3 is not itself directly membrane-associated. Regions of the EF-3 polypeptide also show structural homology with other translation-associated factors including aminoacyl-tRNA synthetases and the Escherichia coli ribosomal protein S5. While the precise role of EF-3 in the translation elongation cycle remains to be defined, recent evidence suggests that it may be involved in optimizing accuracy during mRNA decoding at the ribosomal A site. Furthermore, the essential nature of EF-3 with respect to the fungal cell indicates that it may be an effective antifungal target. Its apparently ubiquitous occurrence throughout the fungal kingdom also suggests that it may be a useful fungal taxonomic marker.


Mol Microbiol


Molecular microbiology


Belfield GP,Tuite MF




Has Abstract


1993-08-01 00:00:00












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    abstract::A unique 373 bp region (igr66) between grpE and dnaK of Streptococcus mutans lacks a promoter but is required for optimal production of DnaK. Northern blotting using probes specific to hrcA, igr66 or dnaK revealed multiple transcripts produced from the dnaK operon and 5'-RACE mapped 5' termini of multiple dnaK transcr...

    journal_title:Molecular microbiology

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    authors: Palmer SR,Burne RA

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    authors: Lester J,Kichler S,Oickle B,Fairweather S,Oberc A,Chahal J,Ratnayake D,Creuzenet C

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    authors: Morales Angeles D,Liu Y,Hartman AM,Borisova M,de Sousa Borges A,de Kok N,Beilharz K,Veening JW,Mayer C,Hirsch AK,Scheffers DJ

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    journal_title:Molecular microbiology

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    authors: Słomińska M,Konopa G,Ostrowska J,Kedzierska B,Wegrzyn G,Wegrzyn A

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    authors: Bruand C,Velten M,McGovern S,Marsin S,Sérèna C,Ehrlich SD,Polard P

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    journal_title:Molecular microbiology

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    authors: van Drunen CM,van Zuylen C,Mientjes EJ,Goosen N,van de Putte P

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  • Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains.

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    authors: Liu B,Deikus G,Bree A,Durand S,Kearns DB,Bechhofer DH

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    authors: Chiang RC,Cavicchioli R,Gunsalus RP

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  • A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor.

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    authors: Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

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    authors: Rico AI,García-Ovalle M,Palacios P,Casanova M,Vicente M

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    pub_type: 杂志文章


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    authors: Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pratt LA,Silhavy TJ

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: van der Ploeg JR,Barone M,Leisinger T

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Flannigan KA,Hennigan SH,Vogelbacker HH,Gots JS,Smith JM

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Mortezaei N,Epler CR,Shao PP,Shirdel M,Singh B,McVeigh A,Uhlin BE,Savarino SJ,Andersson M,Bullitt E

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    abstract::Inhibitors of protein synthesis cause defects in the assembly of ribosomal subunits. In response to treatment with the antibiotics erythromycin or chloramphenicol, precursors of both large and small ribosomal subunits accumulate. We have used a pulse-labelling approach to demonstrate that the accumulating subribosomal...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Siibak T,Peil L,Dönhöfer A,Tats A,Remm M,Wilson DN,Tenson T,Remme J

    更新日期:2011-04-01 00:00:00

  • A novel RNA polymerase-binding protein controlling genes involved in spore germination in Bacillus subtilis.

    abstract::A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Traag BA,Ramirez-Peralta A,Wang Erickson AF,Setlow P,Losick R

    更新日期:2013-07-01 00:00:00

  • Tag-mediated fractionation of yeast ribosome populations proves the monomeric organization of the eukaryotic ribosomal stalk structure.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Guarinos E,Santos C,Sánchez A,Qiu DY,Remacha M,Ballesta JP

    更新日期:2003-10-01 00:00:00

  • Insertion of inverted Ter sites into the terminus region of the Escherichia coli chromosome delays completion of DNA replication and disrupts the cell cycle.

    abstract::To investigate the co-ordination between DNA replication and cell division, we have disrupted the DNA-replication cycle of Escherichia coli by inserting inverted Ter sites into the terminus region to delay completion of the chromosome. The inverted Ter sites (designated InvTer::spcr) were initially inserted into the c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sharma B,Hill TM

    更新日期:1995-10-01 00:00:00

  • Coupling gene expression and multicellular morphogenesis during fruiting body formation in Myxococcus xanthus.

    abstract::A recurring theme in morphogenesis is the coupling of the expression of genes that drive morphogenesis and the morphogenetic process per se. This coupling ensures that gene expression and morphogenesis are carried out in synchrony. Morphogenesis of the spore-filled fruiting bodies in Myxococcus xanthus illustrates thi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Søgaard-Andersen L,Overgaard M,Lobedanz S,Ellehauge E,Jelsbak L,Rasmussen AA

    更新日期:2003-04-01 00:00:00