The hmuUV genes of Sinorhizobium meliloti 2011 encode the permease and ATPase components of an ABC transport system for the utilization of both haem and the hydroxamate siderophores, ferrichrome and ferrioxamine B.


:Sinorhizobium meliloti, the endosymbiont of Medicago sativa, can use haem compounds, including haemoglobin and leghaemoglobin, when growing in the free-living state. The components of the system involved in haem acquisition were confirmed to be ShmR, an outer membrane receptor, and HmuTUV, predicted to be an ABC transport system comprising a periplasmic protein, a permease and an ATPase respectively. The roles of HmuTUV in haem transport were confirmed in a heterologous expression system in Escherichia coli in conjunction with HasR, the outer membrane haem receptor of Serratia marcescens. hmuTUV mutants of S. meliloti showed a reduced capacity to acquire haem, suggesting the presence of a second haem acquisition system in the organism. S. meliloti can also acquire iron from xenosiderophores and the genes encoding the outer membrane receptors for ferrichrome and ferrioxamine B, fhuA1 and fhuA2, respectively, were identified. In light of this it is proposed that fhuA2 should be renamed foxA in the S. meliloti 1021 genome sequence. A siderophore reductase, FhuF, with the capacity to complement an E. coli ferrioxamine B reductase mutant, was identified encoded by a gene next to fhuA2. In the same transcriptional unit as fhuF the gene fhuP was identified and shown to encode a protein necessary for transport of ferrichrome and ferrioxamine B and predicted to be periplasmic. Interestingly, the remaining components of the transport system for the siderophores are HmuU and HmuV. Ferrichrome, ferrioxamine B and haem compounds therefore share components of the same transport system in S. meliloti.


Mol Microbiol


Molecular microbiology


Cuív PO,Keogh D,Clarke P,O'Connell M




Has Abstract


2008-12-01 00:00:00














  • Structural relationship between a bacterial developmental protein and eukaryotic PP2C protein phosphatases.

    abstract::Bacillus subtilis SpoIIE is a Ser protein phosphatase whose action on the phosphoprotein SpoIIAA triggers the cell type-specific activation of a sporulation transcription factor. Here we report that SpoIIE displays sequence similarity to the PP2C family of eukaryotic Ser/Thr protein phosphatases, and that residues com...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Adler E,Donella-Deana A,Arigoni F,Pinna LA,Stragler P

    更新日期:1997-01-01 00:00:00

  • Inactivation of the FNR protein of Escherichia coli by targeted mutagenesis in the N-terminal region.

    abstract::The FNR protein of Escherichia coli is a regulatory protein that activates the transcription of its target genes in response to oxygen limitation. Site-directed mutagenesis was used to show that a 28-residue N-terminal segment containing three cysteines is essential for normal FNR function. The cysteine residue which ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Spiro S,Guest JR

    更新日期:1988-11-01 00:00:00

  • Deciphering the metabolic response of Mycobacterium tuberculosis to nitrogen stress.

    abstract::A key component to the success of Mycobacterium tuberculosis as a pathogen is the ability to sense and adapt metabolically to the diverse range of conditions encountered in vivo, such as oxygen tension, environmental pH and nutrient availability. Although nitrogen is an essential nutrient for every organism, little is...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Williams KJ,Jenkins VA,Barton GR,Bryant WA,Krishnan N,Robertson BD

    更新日期:2015-09-01 00:00:00

  • Altered ParA partition proteins of plasmid P1 act via the partition site to block plasmid propagation.

    abstract::The partition system of the P1 plasmid, P1par, consists of the ParA and ParB proteins and a cis-acting site, parS. It is responsible for the orderly segregation of plasmid copies to daughter cells. Plasmids with null mutations in parA or parB replicate normally, but missegregate. ParB binds specifically to the parS si...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Youngren B,Austin S

    更新日期:1997-09-01 00:00:00

  • Multifunctional analysis of Chlamydia-specific genes in a yeast expression system.

    abstract::Our understanding of how obligate intracellular pathogens co-opt eukaryotic cellular functions has been limited by their intractability to genetic manipulation and by the abundance of pathogen-specific genes with no known functional homologues. In this report we describe a gene expression system to characterize protei...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Sisko JL,Spaeth K,Kumar Y,Valdivia RH

    更新日期:2006-04-01 00:00:00

  • SecY and integral membrane components of the Escherichia coli protein translocation system.

    abstract::Genetic approaches can address the question of how integral membrane Sec factors interact with each other and facilitate protein translocation across the cytoplasmic membrane of E. coli. This review summarizes genetic analyses of SecY, SecE and some other protein translocation factors, utilizing 'prl' mutations, 'sec'...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Ito K

    更新日期:1992-09-01 00:00:00

  • A central regulator of morphological differentiation in the multicellular bacterium Streptomyces coelicolor.

    abstract::In the multicellular bacterium Streptomyces coelicolor, functions of developmental (bald) genes are required for the biosynthesis of SapB, a hydrophobic peptidic morphogen that facilitates aerial hyphae formation. Here, we show that aerial hyphal growth and SapB biosynthesis could be activated independently from the n...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nguyen KT,Willey JM,Nguyen LD,Nguyen LT,Viollier PH,Thompson CJ

    更新日期:2002-12-01 00:00:00

  • Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU.

    abstract::Expression of the gene pcnB, encoding the dispensable Escherichia coli poly(A) polymerase (PAPI), which is toxic when overproduced, was investigated. Its promoter was identified and found to be moderately strong when used to express a beta-galactosidase reporter. Expression levels were not affected by increasing or de...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Binns N,Masters M

    更新日期:2002-06-01 00:00:00

  • Involvement of multiple distinct Bordetella receptor proteins in the utilization of iron liberated from transferrin by host catecholamine stress hormones.

    abstract::Bordetella bronchiseptica is a pathogen that can acquire iron using its native alcaligin siderophore system, but can also use the catechol xenosiderophore enterobactin via the BfeA outer membrane receptor. Transcription of bfeA is positively controlled by a regulator that requires induction by enterobactin. Catecholam...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Armstrong SK,Brickman TJ,Suhadolc RJ

    更新日期:2012-05-01 00:00:00

  • Bacillus subtilis FolE is sustained by the ZagA zinc metallochaperone and the alarmone ZTP under conditions of zinc deficiency.

    abstract::Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Chandrangsu P,Huang X,Gaballa A,Helmann JD

    更新日期:2019-09-01 00:00:00

  • The Rhizobium meliloti exoZl exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM.

    abstract::A 2.6 kb ClaI-BamHI DNA fragment of megaplasmid 2 of Rhizobium meliloti 2011 was found to carry genes involved in exopolysaccharide synthesis and infection of alfalfa nodules. The analysis of the nucleotide sequence of this DNA fragment revealed the existence of two open reading frames (ORFs) running in opposite direc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Buendia AM,Enenkel B,Köplin R,Niehaus K,Arnold W,Pühler A

    更新日期:1991-06-01 00:00:00

  • A potential novel mechanism for the insertion of a membrane protein revealed by a biochemical analysis of the Plasmodium falciparum cytoadherence molecule PfEMP-1.

    abstract::Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is exposed on the surface of infected erythrocytes where it both acts as an important pathogenicity factor in malaria and undergoes antigenic variation as a means of immune evasion. Because the mammalian erythrocyte lacks a protein secretory machinery ther...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Papakrivos J,Newbold CI,Lingelbach K

    更新日期:2005-02-01 00:00:00

  • Vital role for the Plasmodium actin capping protein (CP) beta-subunit in motility of malaria sporozoites.

    abstract::Summary Successful malaria transmission from the mosquito vector to the mammalian host depends crucially on active sporozoite motility. Sporozoite locomotion and host cell invasion are driven by the parasite's own actin/myosin motor. A unique feature of this motor machinery is the presence of very short subpellicular ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ganter M,Schüler H,Matuschewski K

    更新日期:2009-12-01 00:00:00

  • A thiol-disulfide oxidoreductase of the Gram-positive pathogen Corynebacterium diphtheriae is essential for viability, pilus assembly, toxin production and virulence.

    abstract::The Gram-positive pathogen Corynebacterium diphtheriae exports through the Sec apparatus many extracellular proteins that include the key virulence factors diphtheria toxin and the adhesive pili. How these proteins attain their native conformations after translocation as unfolded precursors remains elusive. The fact t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Reardon-Robinson ME,Osipiuk J,Jooya N,Chang C,Joachimiak A,Das A,Ton-That H

    更新日期:2015-12-01 00:00:00

  • Live cell imaging of SOS and prophage dynamics in isogenic bacterial populations.

    abstract::Almost all bacterial genomes contain DNA of viral origin, including functional prophages or degenerated phage elements. A frequent but often unnoted phenomenon is the spontaneous induction of prophage elements (SPI) even in the absence of an external stimulus. In this study, we have analyzed SPI of the large, degenera...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Helfrich S,Pfeifer E,Krämer C,Sachs CC,Wiechert W,Kohlheyer D,Nöh K,Frunzke J

    更新日期:2015-11-01 00:00:00

  • Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.

    abstract::Natural competence in Streptococcus pneumoniae is regulated by a quorum-sensing mechanism consisting of a competence-stimulating peptide (CSP), its dedicated secretion apparatus (ComAB), its histidine kinase receptor (ComD) and a response regulator (ComE). In this report, we show that ComE is a DNA-binding protein tha...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ween O,Gaustad P,Håvarstein LS

    更新日期:1999-08-01 00:00:00

  • The diffusible factor synthase XanB2 is a bifunctional chorismatase that links the shikimate pathway to ubiquinone and xanthomonadins biosynthetic pathways.

    abstract::The diffusible factor synthase XanB2, originally identified in Xanthomonas campestris pv. campestris (Xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2 is a unique bifunctional chorismatase that hydroly...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Zhou L,Wang JY,Wu J,Wang J,Poplawsky A,Lin S,Zhu B,Chang C,Zhou T,Zhang LH,He YW

    更新日期:2013-01-01 00:00:00

  • Nucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506.

    abstract::Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations in penicillin-binding proteins (PBPs) which reduce their affinity for beta-lactam antibiotics. PBP2x is one of the high-Mr PBPs which appears to be altered both in resistant clinical isolates, and in cefotaxime-resistant la...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Laible G,Hakenbeck R,Sicard MA,Joris B,Ghuysen JM

    更新日期:1989-10-01 00:00:00

  • RepC protein of the octopine-type Ti plasmid binds to the probable origin of replication within repC and functions only in cis.

    abstract::Vegetative replication and partitioning of many plasmids and some chromosomes of alphaproteobacteria are directed by their repABC operons. RepA and RepB proteins direct the partitioning of replicons to daughter cells, while RepC proteins are replication initiators, although they do not resemble any characterized repli...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pinto UM,Flores-Mireles AL,Costa ED,Winans SC

    更新日期:2011-09-01 00:00:00

  • The assembly of β-barrel membrane proteins by BAM and SAM.

    abstract::Gram-negative bacteria, mitochondria, and chloroplasts all possess an outer membrane populated with a host of β-barrel outer-membrane proteins (βOMPs). These βOMPs play crucial roles in maintaining viability of their hosts, and therefore, it is essential to understand the biogenesis of this class of membrane proteins....

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lundquist K,Billings E,Bi M,Wellnitz J,Noinaj N

    更新日期:2020-12-13 00:00:00

  • Hop-family Helicobacter outer membrane adhesins form a novel class of Type 5-like secretion proteins with an interrupted β-barrel domain.

    abstract::The human stomach pathogen Helicobacter pyloriattaches to healthy and inflamed gastric tissue through members of a paralogous family of 'Helicobacter outer membrane proteins' (Hops), including adhesins BabA, SabA, HopQ, LabA and HopZ. Hops share a conserved 25 kDa C-terminal region that is thought to form an autotrans...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Coppens F,Castaldo G,Debraekeleer A,Subedi S,Moonens K,Lo A,Remaut H

    更新日期:2018-10-01 00:00:00

  • The hprK gene of Enterococcus faecalis encodes a novel bifunctional enzyme: the HPr kinase/phosphatase.

    abstract::The HPr kinase of Gram-positive bacteria is an ATP-dependent serine protein kinase, which phosphorylates the HPr protein of the bacterial phosphotransferase system (PTS) and is involved in the regulation of carbohydrate metabolism. The hprK gene from Enterococcus faecalis was cloned via polymerase chain reaction (PCR)...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kravanja M,Engelmann R,Dossonnet V,Blüggel M,Meyer HE,Frank R,Galinier A,Deutscher J,Schnell N,Hengstenberg W

    更新日期:1999-01-01 00:00:00

  • Haemolysin-derived synthetic peptides with pore-forming and haemolytic activity.

    abstract::Escherichia coli haemolysin (Hlya) is a pore-forming protein which belongs to the family of 'Repeat-toxins' (RTX) (Lo et al., 1987; Lally et al., 1989; Kraig et al., 1990). A model for the pore-forming structure of HlyA has been proposed (Ludwig et al., 1991) which consists of eight transmembrane segments all present ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Oropeza-Wekerle RL,Muller S,Briand JP,Benz R,Schmid A,Goebel W

    更新日期:1992-01-01 00:00:00

  • Controlling pore assembly of staphylococcal gamma-haemolysin by low temperature and by disulphide bond formation in double-cysteine LukF mutants.

    abstract::Staphylococcal LukF and Hlg2 are water-soluble monomers of gamma-haemolysin that assemble into oligomeric pores on the erythrocyte membranes. Here, we have created double-cysteine LukF mutants, in which single disulphide bonds connect either the prestem domain and the cap domain (V12C-T136C, Cap-Stem), or two beta-str...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nguyen VT,Higuchi H,Kamio Y

    更新日期:2002-09-01 00:00:00

  • Characterization of monoclonal antibodies recognizing three distinct, phosphorylated carbohydrate epitopes in the lipopolysaccharide of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae.

    abstract::Monoclonal antibodies against the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae were obtained after immunization of mice with sheep erythrocytes which had been coated with de-O-acylated LPS. Characterization of antibodies was performed by enzyme immuno assay (EIA) using LPS or...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rozalski A,Brade L,Kosma P,Moxon R,Kusumoto S,Brade H

    更新日期:1997-02-01 00:00:00

  • Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays.

    abstract::Natural genetic transformation in Streptococcus pneumoniae is controlled in part by a quorum-sensing system mediated by a peptide pheromone called competence-stimulating peptide (CSP), which acts to coordinate transient activation of genes required for competence. To characterize the transcriptional response and regul...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Peterson SN,Sung CK,Cline R,Desai BV,Snesrud EC,Luo P,Walling J,Li H,Mintz M,Tsegaye G,Burr PC,Do Y,Ahn S,Gilbert J,Fleischmann RD,Morrison DA

    更新日期:2004-02-01 00:00:00

  • New erythromycin derivatives from Saccharopolyspora erythraea using sugar O-methyltransferases from the spinosyn biosynthetic gene cluster.

    abstract::Using a previously developed expression system based on the erythromycin-producing strain of Saccharopolyspora erythraea, O-methyltransferases from the spinosyn biosynthetic gene cluster of Saccharopolyspora spinosa have been shown to modify a rhamnosyl sugar attached to a 14-membered polyketide macrolactone. The spnI...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gaisser S,Lill R,Wirtz G,Grolle F,Staunton J,Leadlay PF

    更新日期:2001-09-01 00:00:00

  • One if by land, two if by sea: signalling to the ranks with CSP and XIP.

    abstract::In many streptococci, quorum sensing utilizes secreted, linear peptides that engage cognate receptors to coordinate gene expression among members of a local population. Streptococcus mutans employs the secreted peptides CSP and XIP to stimulate production of antimicrobial bacteriocins and to induce development of comp...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Federle MJ,Morrison DA

    更新日期:2012-10-01 00:00:00

  • Three-component-mediated serotype conversion in Pseudomonas aeruginosa by bacteriophage D3.

    abstract::Bacteriophage D3 is capable of lysogenizing Pseudomonas aeruginosa PAO1 (serotype O5), converting the O-antigen from O5 to O16 and O-acetylating the N-acetylfucosamine moiety. To investigate the mechanism of lysogenic conversion, a 3.6 kb fragment from the D3 genome was isolated capable of mediating serotypic conversi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Newton GJ,Daniels C,Burrows LL,Kropinski AM,Clarke AJ,Lam JS

    更新日期:2001-03-01 00:00:00

  • Disruption of the GABA shunt affects mitochondrial respiration and virulence in the cereal pathogen Fusarium graminearum.

    abstract::The cereal pathogen Fusarium graminearum threatens food and feed production worldwide. It reduces the yield and poisons the remaining kernels with mycotoxins, notably deoxynivalenol (DON). We analyzed the importance of gamma-aminobutanoic acid (GABA) metabolism for the life cycle of this fungal pathogen. GABA metaboli...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bönnighausen J,Gebhard D,Kröger C,Hadeler B,Tumforde T,Lieberei R,Bergemann J,Schäfer W,Bormann J

    更新日期:2015-12-01 00:00:00