Characterization of the CheAS/CheZ complex: a specific interaction resulting in enhanced dephosphorylating activity on CheY-phosphate.

abstract：:The invasive phenotype of Shigella flexneri is conferred by a 220 kb virulence plasmid, pWR100, that encodes both the lpa proteins, which are involved in the entry process, and factors which are required for the export and correct localization of the lpa proteins. We have characterized the mxiD gene, whose expression,...

journal_title：Molecular microbiology

authors： Allaoui A,Sansonetti PJ,Parsot C

更新日期：1993-01-01 00:00:00

abstract：:The interactions between bacterial pathogens and their eukaryotic hosts are vital in determining the outcome of infections. Bacterial pathogens employ molecular sensors to detect and facilitate adaptation to changes in their niche. The sensing of these extracellular signals enables the pathogen to navigate within mamm...

journal_title：Molecular microbiology

authors： Karavolos MH,Winzer K,Williams P,Khan CM

更新日期：2013-02-01 00:00:00

abstract：:Transposases mediate transposition first by binding specific DNA end sequences that define a transposable element and then by organizing protein and DNA into a highly structured and stable nucleoprotein 'synaptic' complex. Synaptic complex assembly is a central checkpoint in many transposition mechanisms. The Tn5 syna...

journal_title：Molecular microbiology

authors： Adams CD,Schnurr B,Skoko D,Marko JF,Reznikoff WS

更新日期：2006-12-01 00:00:00

abstract：:The 136 codon (408 bp) denA gene encoding endonuclease II (Endoll) of bacteriophage T4 was unambiguously identified through sequencing and subsequent cloning. Endoll prepared from cloned DNA through coupled in vitro transcription-translation nicked and cut DNA in vitro in a sequence-specific manner. In vitro (and in v...

journal_title：Molecular microbiology

authors： Carlson K,Kosturko LD,Nyström AC

更新日期：1999-03-01 00:00:00

abstract：:The mechanisms of transcription repression and derepression in vivo are not fully understood. We have obtained evidence that begins to clarify the minimum requirements for counteracting nucleosomal repression in vivo. Location of the TATA element near the nucleosome dyad does not block RNA polymerase II transcription ...

journal_title：Molecular microbiology

authors： Moss DR,Laybourn PJ

更新日期：2000-06-01 00:00:00

abstract：:We recently identified a gene (FTN_0818) required for Francisella virulence that seemed likely involved in biotin metabolism. However, the molecular function of this virulence determinant was unclear. Here we show that this protein named BioJ is the enzyme of the biotin biosynthesis pathway that determines the chain l...

journal_title：Molecular microbiology

authors： Feng Y,Napier BA,Manandhar M,Henke SK,Weiss DS,Cronan JE

更新日期：2014-01-01 00:00:00

abstract：:The most abundant oxidants controlling bacterial colonization on mucosal barrier epithelia are hypochlorous acid (HOCl), hypobromous acid (HOBr) and hypothiocyanous acid (HOSCN). All three oxidants are highly antimicrobial but little is known about their relative efficacies, their respective cellular targets, or what ...

journal_title：Molecular microbiology

authors： Groitl B,Dahl JU,Schroeder JW,Jakob U

更新日期：2017-11-01 00:00:00

abstract：:We have characterized a number of mutations in fnr that interfere with FNR-dependent transcription activation at two promoters where the FNR-binding site is centred around 41 1/2 bp upstream from the transcription start site. The substituted residues in all but one of these FNR mutants are clustered around a presumed ...

journal_title：Molecular microbiology

authors： Bell A,Busby S

更新日期：1994-01-01 00:00:00

abstract：:Although once thought to be unique to bacteria, d-amino acids are also produced by mammals. For example, d-serine is excreted in human urine at concentrations ranging from 3.0 to 40 micro g ml-1. An epidemiological survey demonstrated that urine isolates of E. coli are more likely to catabolise d-serine via expression...

journal_title：Molecular microbiology

authors： Roesch PL,Redford P,Batchelet S,Moritz RL,Pellett S,Haugen BJ,Blattner FR,Welch RA

更新日期：2003-07-01 00:00:00

abstract：:A number of strains of the widespread aerobic soil bacterium, Bacillus sphaericus, possess crystalline inclusions of a toxin lethal to a variety of insect (larvae) which are vectors of major tropical diseases. Partial amino acid sequence data from one strain, B. sphaericus 2362 have permitted us to design oligonucleot...

journal_title：Molecular microbiology

authors： Hindley J,Berry C

更新日期：1987-09-01 00:00:00

abstract：:Staphylococcus aureus is an important pathogen that continues to be a significant global health threat because of the prevalence of methicillin-resistant S. aureus strains (MRSA). The pathogenesis of this organism is partly attributed to the production of a large repertoire of cytotoxins that target and kill innate im...

journal_title：Molecular microbiology

authors： Dumont AL,Nygaard TK,Watkins RL,Smith A,Kozhaya L,Kreiswirth BN,Shopsin B,Unutmaz D,Voyich JM,Torres VJ

更新日期：2011-02-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:The bioluminescent marine bacterium Vibrio harveyi controls light production (lux) by an elaborate quorum-sensing circuit. V. harveyi produces and responds to two different autoinducer signals (AI-1 and AI-2) to modulate the luciferase structural operon (luxCDABEGH) in response to changes in cell-population density. U...

journal_title：Molecular microbiology

authors： Lilley BN,Bassler BL

更新日期：2000-05-01 00:00:00

abstract：:It was demonstrated recently that the SeqA protein, a main negative regulator of Escherichia coli chromosome replication initiation, is also a specific transcription factor. SeqA specifically activates the bacteriophage lambda pR promoter while revealing no significant effect on the activity of another lambda promoter...

journal_title：Molecular microbiology

authors： Słomińska M,Konopa G,Ostrowska J,Kedzierska B,Wegrzyn G,Wegrzyn A

更新日期：2003-03-01 00:00:00

abstract：:Ribonucleases J1 and J2 of Bacillus subtilis are evolutionarily conserved enzymes combining an endoribonucleolytic and a 5'-3' exoribonucleolytic activity in a single polypeptide. Their endoribonucleolytic cleavage specificity resembles that of RNase E, a key player in the processing and degradation of RNA in Escheric...

journal_title：Molecular microbiology

authors： Mäder U,Zig L,Kretschmer J,Homuth G,Putzer H

更新日期：2008-10-01 00:00:00

abstract：:Sinorhizobium meliloti and host legumes enter into a nitrogen-fixing, symbiotic relationship triggered by an exchange of signals between bacteria and plant. S. meliloti produces Nod factor, which elicits the formation of nodules on plant roots, and succinoglycan, an exopolysaccharide that allows for bacterial invasion...

journal_title：Molecular microbiology

authors： Wells DH,Long SR

更新日期：2002-03-01 00:00:00

abstract：:The flow of information from the outside to the inside of bacterial cells is largely directed by protein kinases. In addition to histidine/aspartate phosphorelays of two-component response regulators, recent work in Mycobacterium tuberculosis (Mtb) reinforces the idea that phosphorylation on serine (Ser), threonine (T...

journal_title：Molecular microbiology

authors： Sherman DR,Grundner C

更新日期：2014-10-01 00:00:00

abstract：:Replication fork arrest can cause DNA double-strand breaks (DSBs). These DSBs are caused by the action of the Holliday junction resolvase RuvABC, indicating that they are made by resolution of Holliday junctions formed at blocked forks. In this work, we study the homologous recombination functions required for RuvABC-...

journal_title：Molecular microbiology

authors： Seigneur M,Ehrlich SD,Michel B

更新日期：2000-11-01 00:00:00

abstract：:Cells invest a significant amount of their energy synthesizing proteins, and a large portion of the energy expenditure goes into making ribosomes, the RNA-protein machines at the centre of translation. When ribosomes are damaged in a cell, i.e. during stressful conditions, cells must first recognize the damage and the...

journal_title：Molecular microbiology

authors： Cate JH

更新日期：2010-02-01 00:00:00

abstract：:Candida albicans hyphae grow in a highly polarized fashion from their tips. This polarized growth requires the continuous delivery of secretory vesicles to the tip region. Vesicle delivery depends on Sec2p, the Guanine Exchange Factor (GEF) for the Rab GTPase Sec4p. GTP bound Sec4p is required for the transit of secre...

journal_title：Molecular microbiology

authors： Caballero-Lima D,Hautbergue GM,Wilson SA,Sudbery PE

更新日期：2014-11-01 00:00:00

abstract：:We have isolated and sequenced a set of deletions stimulated by DNA palindromes in Escherichia coli. All of the deletions are asymmetric with respect to the parental sequence and have occurred at short direct repeats. This is consistent with deletion by strand slippage during DNA replication. The orientation of the as...

journal_title：Molecular microbiology

authors： Pinder DJ,Blake CE,Lindsey JC,Leach DR

更新日期：1998-05-01 00:00:00

abstract：:We show in this work that disruption of the hfq gene in Escherichia coli causes a large increase in IS10 transposition when IS10 is present on a multi-copy plasmid. Hfq is an RNA-binding protein that regulates the expression of a large number of genes at the post-transcriptional level by promoting the pairing of mRNAs...

journal_title：Molecular microbiology

authors： Ross JA,Wardle SJ,Haniford DB

更新日期：2010-11-01 00:00:00

abstract：:Cpmk2, encoding a mitogen-activated protein (MAP) kinase from the ascomycete Claviceps purpurea, is an orthologue of SLT2 from Saccharomyces cerevisiae, the first isolated from a biotrophic, non-appressorium-forming pathogen. Deletion mutants obtained by a gene replacement approach show impaired vegetative properties ...

journal_title：Molecular microbiology

authors： Mey G,Held K,Scheffer J,Tenberge KB,Tudzynski P

更新日期：2002-10-01 00:00:00

abstract：:The mitis group streptococci (MGS) are widespread in the oral cavity and are traditionally associated with oral health. However, these organisms have many attributes that contribute to the development of pathogenic oral communities. MGS adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment su...

journal_title：Molecular microbiology

authors： Whitmore SE,Lamont RJ

更新日期：2011-07-01 00:00:00

abstract：:SpsA, a pneumococcal surface protein belonging to the family of choline-binding proteins, interacts specifically with secretory immunglobulin A (SIgA) via the secretory component (SC). SIgA and free SC from mouse, rat, rabbit and guinea-pig failed to interact with SpsA indicating species-specific binding to human SIgA...

journal_title：Molecular microbiology

authors： Hammerschmidt S,Tillig MP,Wolff S,Vaerman JP,Chhatwal GS

更新日期：2000-05-01 00:00:00

abstract：:Borrelia burgdorferi survives in an enzootic cycle, and Dps proteins protect DNA against damage during starvation or oxidative stress. The role of a Dps homologue encoded by Borrelia in spirochaete survival was assessed. Dps-deficient spirochaetes were infectious in mice via needle-inoculation at the dose of 10(5) spi...

journal_title：Molecular microbiology

authors： Li X,Pal U,Ramamoorthi N,Liu X,Desrosiers DC,Eggers CH,Anderson JF,Radolf JD,Fikrig E

更新日期：2007-02-01 00:00:00

abstract：:The liver stage is the first stage of the malaria parasite that replicates in the vertebrate host. However, little is known about the interplay between the parasite liver stage and its host cell, the hepatocyte. In this study, we identified an exported protein that has a critical role in parasite development in host h...

journal_title：Molecular microbiology

authors： Orito Y,Ishino T,Iwanaga S,Kaneko I,Kato T,Menard R,Chinzei Y,Yuda M

更新日期：2013-01-01 00:00:00

abstract：:The AbcR small RNAs (sRNAs) are a fascinating example of two highly conserved sRNAs that differ tremendously at the functional level among organisms. From their transcriptional activation to their regulatory capabilities, the AbcR sRNAs exhibit varying characteristics in three well-studied bacteria belonging to the Rh...

journal_title：Molecular microbiology

authors： Sheehan LM,Caswell CC

更新日期：2018-01-01 00:00:00

abstract：:The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this oc...

journal_title：Molecular microbiology

authors： Alao JP,Huis In 't Veld PJ,Buhse F,Sunnerhagen P

更新日期：2010-07-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00