Upstream nucleosomes and Rgr1p are required for nucleosomal repression of transcription.

abstract：:Streptococcus lactis NCDO763 harbours a plasmid designated pLP763. The cells harbouring pLP763 are able to grow to a higher density in milk because of their proteinase-positive phenotype (Prt+). The 6.2 kb HindIII-PstI fragment from pLP763 was found to be responsible for the Prt+ phenotype. The DNA fragment contains a...

journal_title：Molecular microbiology

authors： Kiwaki M,Ikemura H,Shimizu-Kadota M,Hirashima A

更新日期：1989-03-01 00:00:00

abstract：:The six biochemical steps of the de novo pyrimidine biosynthesis pathway are conserved in all known organisms. However, in animals and fungi, unlike prokaryotes, at least the first two activities are grouped on a multifunctional enzyme. Here, we report cloning, mapping and transcriptional characterization of some pyri...

journal_title：Molecular microbiology

authors： Aleksenko A,Liu W,Gojkovic Z,Nielsen J,Piskur J

更新日期：1999-08-01 00:00:00

abstract：:The Bacillus subtilis GlnR transcription factor regulates gene expression in response to changes in nitrogen availability. Glutamine synthetase transmits the nitrogen regulatory signal to GlnR. The DNA-binding activity of GlnR is activated by a transient protein-protein interaction with feedback-inhibited glutamine sy...

journal_title：Molecular microbiology

authors： Wray LV Jr,Fisher SH

更新日期：2008-04-01 00:00:00

abstract：:Bacterial cytochrome cbb3 oxidases are members of the haeme-copper oxidase superfamily that are important for energy conservation by a variety of proteobacteria under oxygen-limiting conditions. The opportunistic pathogen Pseudomonas aeruginosa is unusual in possessing two operons that each potentially encode a cbb3 o...

journal_title：Molecular microbiology

authors： Comolli JC,Donohue TJ

更新日期：2004-02-01 00:00:00

abstract：:Escherichia coli RnlA-RnlB is a newly identified toxin-antitoxin (TA) system that plays a role in bacteriophage resistance. RnlA functions as a toxin with mRNA endoribonuclease activity and the cognate antitoxin RnlB inhibits RnlA toxicity in E. coli cells. Interestingly, T4 phage encodes the antitoxin Dmd, which acts...

journal_title：Molecular microbiology

authors： Wei Y,Gao ZQ,Otsuka Y,Naka K,Yonesaki T,Zhang H,Dong YH

更新日期：2013-12-01 00:00:00

abstract：:Filamentous heterocyst-forming cyanobacteria are a beautiful example of prokaryotic multicellularity. The filaments can achieve simultaneous nitrogen fixation and oxygenic photosynthesis by cooperation between two cell types: the photosynthetic vegetative cells and the nitrogen-fixing heterocysts. The multicellular fe...

journal_title：Molecular microbiology

authors： Mullineaux CW,Nürnberg DJ

更新日期：2014-12-01 00:00:00

abstract：:Transmission of Plasmodium species from a mammalian host to the mosquito vector requires the uptake, during an infected blood meal, of gametocytes, the precursor cells of the gametes. Relatively little is known about the molecular mechanisms involved in the developmental switch from asexual development to sexual diffe...

journal_title：Molecular microbiology

authors： Boisson B,Lacroix C,Bischoff E,Gueirard P,Bargieri DY,Franke-Fayard B,Janse CJ,Ménard R,Baldacci P

更新日期：2011-09-01 00:00:00

abstract：:Gram-negative bacteria, mitochondria, and chloroplasts all possess an outer membrane populated with a host of β-barrel outer-membrane proteins (βOMPs). These βOMPs play crucial roles in maintaining viability of their hosts, and therefore, it is essential to understand the biogenesis of this class of membrane proteins....

journal_title：Molecular microbiology

authors： Lundquist K,Billings E,Bi M,Wellnitz J,Noinaj N

更新日期：2020-12-13 00:00:00

abstract：:It has previously been proposed that Escherichia coli ribosomal protein S1 is required for the translation of highly structured mRNAs. In this study, we have examined the influence of structural features at or near the start codon of different mRNAs. The requirement for ribosomal protein S1 for translation initiation ...

journal_title：Molecular microbiology

authors： Tedin K,Resch A,Bläsi U

更新日期：1997-07-01 00:00:00

abstract：:In this issue of Molecular Microbiology, Rugarabamu and colleagues investigate the role of rhomboid proteases responsible for adhesin shedding during invasion of the apicomplexan parasite Toxoplasma gondii. This study, together with several other recent publications, raises new questions about the function of these rh...

journal_title：Molecular microbiology

authors： Stewart RJ,Tonkin CJ

更新日期：2015-07-01 00:00:00

abstract：:The unfolded protein response (UPR) is an important stress signalling pathway involved in the cellular development and environmental adaptation of fungi. We investigated the importance of the UPR pathway in the pathogenicity of the plant necrotrophic fungus Alternaria brassicicola, which causes black spot disease on a...

journal_title：Molecular microbiology

authors： Joubert A,Simoneau P,Campion C,Bataillé-Simoneau N,Iacomi-Vasilescu B,Poupard P,François JM,Georgeault S,Sellier E,Guillemette T

更新日期：2011-03-01 00:00:00

abstract：:Genes acrAB encode a multidrug efflux pump in Escherichia coli. We have previously reported that transcription of acrAB is increased under general stress conditions (i.e. 4% ethanol, 0.5 M NaCl, and the stationary phase in Luria-Bertani medium). In this study, lacZ transcriptional fusions and an in vitro gel mobility ...

journal_title：Molecular microbiology

authors： Ma D,Alberti M,Lynch C,Nikaido H,Hearst JE

更新日期：1996-01-01 00:00:00

abstract：:Transposon insertion mutagenesis and transformation were used to locate genes responsible for excision in the temperature phage HP1 of Haemophilus influenzae. A 6.5 kb segment of DNA near the left end of the phage genome was sequenced, and 11 new open reading frames were identified. Two face-to-face overlapping promot...

journal_title：Molecular microbiology

authors： Esposito D,Scocca JJ

更新日期：1994-08-01 00:00:00

abstract：:The stalk is a universal structure of the large ribosomal subunit involved in the function of translation factors. The bacterial stalk is highly stable but its stability is notably reduced in eukaryotes, favouring a translation regulatory activity of this ribosomal domain, which has not been reported in prokaryotes. T...

journal_title：Molecular microbiology

authors： Santos C,Ballesta JP

更新日期：2005-10-01 00:00:00

abstract：:Natural sequence variation was investigated among serogroup A subgroup IV-1 Neisseria meningitidis isolated from diseased patients and healthy carriers in The Gambia, West Africa. The frequencies of DNA import were analysed by sequencing fragments of four linked genes encoding the immunogenic outer membrane proteins T...

journal_title：Molecular microbiology

authors： Linz B,Schenker M,Zhu P,Achtman M

更新日期：2000-06-01 00:00:00

abstract：:The structures of the choline-dependent pneumococcal murein hydrolases, LYTA amidase and CPL1 lysozyme, and the choline-independent CPL7 lysozyme were analysed by controlled proteolytic digestions. The trypsin cleavage of the CPL1 and CPL7 lysozymes produced two resistant polypeptides, F1 and F7 respectively, correspo...

journal_title：Molecular microbiology

authors： Sanz JM,Díaz E,García JL

更新日期：1992-04-01 00:00:00

abstract：:The bacterium Burkholderia glumae causes rice grain rot by producing toxoflavin, whose expression is regulated by quorum sensing (QS). We report a major deviation from the current paradigm for the regulation of bacterial polar flagellum genes. The N-octanoyl homoserine lactone (C8-HSL)-deficient mutant of B. glumae is...

journal_title：Molecular microbiology

authors： Kim J,Kang Y,Choi O,Jeong Y,Jeong JE,Lim JY,Kim M,Moon JS,Suga H,Hwang I

更新日期：2007-04-01 00:00:00

abstract：:NsrR is a nitric oxide (NO)-sensitive transcription repressor that controls NO metabolism in a wide range of bacteria. In Bacillus subtilis, NsrR represses transcription of the nitrite reductase (nasDEF) genes that are under positive control of the ResD-ResE two-component signal transduction system. Derepression is ac...

journal_title：Molecular microbiology

authors： Kommineni S,Yukl E,Hayashi T,Delepine J,Geng H,Moënne-Loccoz P,Nakano MM

更新日期：2010-12-01 00:00:00

abstract：:Cpmk2, encoding a mitogen-activated protein (MAP) kinase from the ascomycete Claviceps purpurea, is an orthologue of SLT2 from Saccharomyces cerevisiae, the first isolated from a biotrophic, non-appressorium-forming pathogen. Deletion mutants obtained by a gene replacement approach show impaired vegetative properties ...

journal_title：Molecular microbiology

authors： Mey G,Held K,Scheffer J,Tenberge KB,Tudzynski P

更新日期：2002-10-01 00:00:00

abstract：:Shigella flexneri kills macrophages through apoptosis, involving the induction of host cell DNA fragmentation and characteristic morphological changes. Shigella can only cause damage if it escapes from the phagolysosome into the cytoplasm. The S. flexneri cytotoxic genes have been localized to the ipa operon of shigel...

journal_title：Molecular microbiology

authors： Zychlinsky A,Kenny B,Ménard R,Prévost MC,Holland IB,Sansonetti PJ

更新日期：1994-02-01 00:00:00

abstract：:The energy requirement for the second step in pullulanase secretion by the general secretory pathway was studied in Escherichia coli. In order to uncouple the two steps in the secretion pathway (across the cytoplasmic and outer membranes, respectively) and to facilitate kinetic analysis of secretion, a variant form of...

journal_title：Molecular microbiology

authors： Possot OM,Letellier L,Pugsley AP

更新日期：1997-05-01 00:00:00

abstract：:Arsenic is the most ubiquitous environmental toxin. Here, we demonstrate that bacteria have evolved the ability to use arsenic to gain a competitive advantage over other bacteria at least twice. Microbes generate toxic methylarsenite (MAs(III)) by methylation of arsenite (As(III)) or reduction of methylarsenate (MAs(V...

journal_title：Molecular microbiology

authors： Chen J,Yoshinaga M,Rosen BP

更新日期：2019-02-01 00:00:00

abstract：:Beta-oxidation (beta-ox) occurs exclusively in the peroxisomes of Saccharomyces cerevisiae and other yeasts, leading to the supposition that fungi lack mitochondrial beta-ox. Here we present unequivocal evidence that the filamentous fungus Aspergillus nidulans houses both peroxisomal and mitochondrial beta-ox. While g...

journal_title：Molecular microbiology

authors： Maggio-Hall LA,Keller NP

更新日期：2004-12-01 00:00:00

abstract：:In nature, organic acids are a commonly used source of carbon and energy. Many bacteria use AMP-forming acid:CoA ligases to convert organic acids into their corresponding acyl-CoA derivatives, which can then enter metabolism. The soil environment contains a broad diversity of organic acids, so it is not surprising tha...

journal_title：Molecular microbiology

authors： Burckhardt RM,VanDrisse CM,Tucker AC,Escalante-Semerena JC

更新日期：2020-01-01 00:00:00

abstract：:Fluorescent polyclonal antibodies specific for MukB have been used to study its localization in Escherichia coli. In wild-type cells, the MukB protein appeared as a limited number of oblong shapes embracing the nucleoid. MukB remained associated with the nucleoid in the absence of DNA replication. The centre of gravit...

journal_title：Molecular microbiology

authors： den Blaauwen T,Lindqvist A,Löwe J,Nanninga N

更新日期：2001-12-01 00:00:00

abstract：:The type III secretion system (TTSS) is a modular apparatus assembled by many pathogenic Gram-negative bacteria and is designed to translocate proteins through the bacterial cell wall into the eukaryotic host cell. The conserved components of the TTSS comprise stacks of rings spanning the inner and outer bacterial mem...

journal_title：Molecular microbiology

authors： Daniell SJ,Kocsis E,Morris E,Knutton S,Booy FP,Frankel G

更新日期：2003-07-01 00:00:00

abstract：:The Helicobacter pylori vacuolating cytotoxin (VacA) induces degenerative vacuolation of sensitive mammalian cell lines. Although evidence is accumulating that VacA enters cells and functions from an intracellular site of action, the biochemical mechanism by which VacA mediates cellular vacuolation has not been establ...

journal_title：Molecular microbiology

authors： Ye D,Blanke SR

更新日期：2002-03-01 00:00:00

abstract：:The TonB system of Escherichia coli (TonB/ExbB/ExbD) transduces the protonmotive force (pmf) of the cytoplasmic membrane to drive active transport by high-affinity outer membrane transporters. In this study, chromosomally encoded ExbD formed formaldehyde-linked complexes with TonB, ExbB and itself (homodimers) in vivo...

journal_title：Molecular microbiology

authors： Ollis AA,Manning M,Held KG,Postle K

更新日期：2009-08-01 00:00:00

abstract：:We have isolated RNA polymerase from Mycobacterium smegmatis and established conditions for specific transcription initiation in vitro. The M. smegmatis enzyme has a strong dependence on supercoiling of the DNA substrate for transcription from mycobacterial promoters. We also show that RNA polymerase is the target for...

journal_title：Molecular microbiology

authors： Levin ME,Hatfull GF

更新日期：1993-04-01 00:00:00

abstract：:The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no ...

journal_title：Molecular microbiology

authors： Westermayer SA,Fritz G,Gutiérrez J,Megerle JA,Weißl MP,Schnetz K,Gerland U,Rädler JO

更新日期：2016-05-01 00:00:00