The assembly of β-barrel membrane proteins by BAM and SAM.

abstract：:We have used a targeted gene deletion event to remove the coding region for the bgl1 gene encoding an extracellular beta-glucosidase from the genome of the cellulolytic fungus Trichoderma reesei. The bgl1 null mutants were used to investigate the role of beta-glucosidase in the hydrolysis of cellulose and induction of...

journal_title：Molecular microbiology

authors： Fowler T,Brown RD Jr

更新日期：1992-11-01 00:00:00

abstract：:Messenger RNA decay in Bacillus subtilis is accomplished by a combination of exoribonucleases and endoribonucleases. Intermediates in the decay process have not been readily detectable, and previous studies on mRNA decay have used a handful of highly expressed transcripts as models. Here, we use RNA-Seq analysis to pr...

journal_title：Molecular microbiology

authors： Liu B,Deikus G,Bree A,Durand S,Kearns DB,Bechhofer DH

更新日期：2014-10-01 00:00:00

abstract：:Bacteria react to phosphate starvation by activating genes involved in the transport and assimilation of phosphate as well as other phosphorous compounds. Some soil bacteria have evolved an additional mechanism for saving phosphorous. Under phosphate-limiting conditions, they replace their membrane phospholipids by li...

journal_title：Molecular microbiology

authors： Geiger O,Röhrs V,Weissenmayer B,Finan TM,Thomas-Oates JE

更新日期：1999-04-01 00:00:00

abstract：:MexAB-OprM is a multidrug efflux system that contributes to intrinsic and acquired multidrug resistance in Pseudomonas aeruginosa, the latter as a result of mutational hyperexpression of the mexAB-oprM operon. While efflux gene hyperexpression typically results from mutations in the linked mexR repressor gene, it also...

journal_title：Molecular microbiology

authors： Cao L,Srikumar R,Poole K

更新日期：2004-09-01 00:00:00

abstract：:Listeria monocytogenes is an intracellular bacterial pathogen that expresses several surface proteins critical for the infectious process. Such proteins include InlA (internalin) and InlB, involved in bacterial entry into the host cell, and ActA, required for bacterially induced actin-based motility. Although the mole...

journal_title：Molecular microbiology

authors： Braun L,Dramsi S,Dehoux P,Bierne H,Lindahl G,Cossart P

更新日期：1997-07-01 00:00:00

abstract：:Phycobilisomes are the major constituents of the light-harvesting apparatus in both cyanobacteria and red algae and consist of a central core with radiating rods. From a genomic library of the cyanobacterium Calothrix 7601, a DNA fragment encoding allophycocyanin B, one of the two terminal energy acceptors of the core...

journal_title：Molecular microbiology

authors： Houmard J,Capuano V,Coursin T,Tandean de Marsac N

更新日期：1988-01-01 00:00:00

abstract：:Fourteen Rhodobacter capsulatus mutants unable to grow with xanthine as sole nitrogen source were isolated by random Tn5 mutagenesis. Five of these Tn5 insertions were mapped within two adjacent chromosomal EcoRI fragments hybridizing to oligonucleotides synthesized according to conserved amino acid sequences of eukar...

journal_title：Molecular microbiology

authors： Leimkühler S,Kern M,Solomon PS,McEwan AG,Schwarz G,Mendel RR,Klipp W

更新日期：1998-02-01 00:00:00

abstract：:While bacteria such as Escherichia coli and Bacillus subtilis harbour a single circular chromosome, some freshwater cyanobacteria have multiple chromosomes p er cell. The detailed mechanism(s) of cyanobacterialreplication remains unclear. To elucidate the replication origin (ori ), form and synchrony of the multi-copy...

journal_title：Molecular microbiology

authors： Watanabe S,Ohbayashi R,Shiwa Y,Noda A,Kanesaki Y,Chibazakura T,Yoshikawa H

更新日期：2012-02-01 00:00:00

abstract：:Polycyclic aromatic hydrocarbons (PAHs) are persistent and toxic environmental pollutants that accumulate in anoxic habitats. With the exception of naphthalene, nothing is known about the microbial degradation of PAH in these environments. The challenge that must be met in anaerobic PAH degradation is the destabilizat...

journal_title：Molecular microbiology

authors： Sawers RG

更新日期：2015-01-01 00:00:00

abstract：:A fragment of mitochondrial DNA containing the Kluyveromyces lactis gene for valine-tRNA (tRNAVAL) was isolated as a multicopy suppressor of a respiratory-deficient mutant of this yeast. The mutant produced a truncated Cox14p because of a +1 frameshift mutation in COX14, a nuclear gene encoding a protein imported into...

journal_title：Molecular microbiology

authors： Fiori A,Francisci S,Falcone C

更新日期：2002-10-01 00:00:00

abstract：:The transmembrane protein ToxR plays a key role in the virulence expression system of Vibrio cholerae. The activity of ToxR is dependent on its periplasmic sensor domain (ToxRp) and on the inner membrane protein ToxS. Herein, we present the Nuclear Magnetic Resonance NMR solution structure of the sensory ToxRp contain...

journal_title：Molecular microbiology

authors： Gubensäk N,Wagner GE,Schrank E,Falsone FS,Berger TMI,Pavkov-Keller T,Reidl J,Zangger K

更新日期：2020-12-23 00:00:00

abstract：:In Saccharomyces cerevisiae, glucose activation of cAMP synthesis requires both the presence of the G-protein-coupled receptor (GPCR) system, Gpr1-Gpa2, and uptake and phosphorylation of the sugar. In a hxt-null strain that lacks all physiologically important glucose carriers, glucose transport as well as glucose-indu...

journal_title：Molecular microbiology

authors： Rolland F,De Winde JH,Lemaire K,Boles E,Thevelein JM,Winderickx J

更新日期：2000-10-01 00:00:00

abstract：:The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generated chromosomal frag...

journal_title：Molecular microbiology

authors： Follettie MT,Shin HK,Sinskey AJ

更新日期：1988-01-01 00:00:00

abstract：:Myxococcus xanthus is a Gram-negative, soil-dwelling bacterium with a complex life cycle which includes fruiting body formation and sporulation in response to starvation. This developmental process is slow, requiring a minimum of 24-48 h, and requires cells to be at high cell density on a solid surface. It is known th...

journal_title：Molecular microbiology

authors： O'Connor KA,Zusman DR

更新日期：1997-05-01 00:00:00

abstract：:The MtrCDE multidrug pump, from Neisseria gonorrhoeae, is assembled from the inner and outer membrane proteins MtrD and MtrE, which are connected by the periplasmic membrane fusion protein MtrC. Although it is clear that MtrD delivers drugs to the channel of MtrE, it remains unclear how drug delivery and channel openi...

journal_title：Molecular microbiology

authors： Janganan TK,Bavro VN,Zhang L,Borges-Walmsley MI,Walmsley AR

更新日期：2013-05-01 00:00:00

abstract：:Iron is a critical nutrient for the growth and survival of most bacterial species. Accordingly, much attention has been paid to the mechanisms by which host organisms sequester iron from invading bacteria and how bacteria acquire iron from their environment. However, under oxidative stress conditions such as those enc...

journal_title：Molecular microbiology

authors： Frawley ER,Fang FC

更新日期：2014-08-01 00:00:00

abstract：:Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identifica...

journal_title：Molecular microbiology

authors： Thomas JA,Weintraub ST,Wu W,Winkler DC,Cheng N,Steven AC,Black LW

更新日期：2012-04-01 00:00:00

abstract：:The NarL and NarP proteins are homologous response regulators of Escherichia coli that control the expression of several operons in response to nitrate and nitrite. A consensus heptameric NarL DNA-binding sequence has been identified, and previous observations suggest that the NarP protein has a similar sequence speci...

journal_title：Molecular microbiology

authors： Darwin AJ,Tyson KL,Busby SJ,Stewart V

更新日期：1997-08-01 00:00:00

abstract：:The invasive phenotype of Shigella flexneri is conferred by a 220 kb virulence plasmid, pWR100, that encodes both the lpa proteins, which are involved in the entry process, and factors which are required for the export and correct localization of the lpa proteins. We have characterized the mxiD gene, whose expression,...

journal_title：Molecular microbiology

authors： Allaoui A,Sansonetti PJ,Parsot C

更新日期：1993-01-01 00:00:00

abstract：:The energy requirement for the second step in pullulanase secretion by the general secretory pathway was studied in Escherichia coli. In order to uncouple the two steps in the secretion pathway (across the cytoplasmic and outer membranes, respectively) and to facilitate kinetic analysis of secretion, a variant form of...

journal_title：Molecular microbiology

authors： Possot OM,Letellier L,Pugsley AP

更新日期：1997-05-01 00:00:00

abstract：:Strains of Neisseria meningitidis express one of two porin proteins. These porins have been identified as the class 2 and class 3 proteins, and express serotype-specific epitopes. The gene for the class 3 protein was amplified by the polymerase chain reaction from the DNA of a serotype 4 strain as a 1025 bp fragment. ...

journal_title：Molecular microbiology

authors： Zapata GA,Vann WF,Rubinstein Y,Frasch CE

更新日期：1992-12-01 00:00:00

abstract：:Bacteriophage D3 is capable of lysogenizing Pseudomonas aeruginosa PAO1 (serotype O5), converting the O-antigen from O5 to O16 and O-acetylating the N-acetylfucosamine moiety. To investigate the mechanism of lysogenic conversion, a 3.6 kb fragment from the D3 genome was isolated capable of mediating serotypic conversi...

journal_title：Molecular microbiology

authors： Newton GJ,Daniels C,Burrows LL,Kropinski AM,Clarke AJ,Lam JS

更新日期：2001-03-01 00:00:00

abstract：:The mobile insertion sequence, IS6110, is an important marker in tracking of Mycobacterium tuberculosis strains. Here, we demonstrate that IS6110 can upregulate downstream genes through an outward-directed promoter in its 3' end, thus adding to the significance of this element. Promoter activity was orientation depend...

journal_title：Molecular microbiology

authors： Safi H,Barnes PF,Lakey DL,Shams H,Samten B,Vankayalapati R,Howard ST

更新日期：2004-05-01 00:00:00

abstract：:Only few small, regulatory RNAs encoded opposite another gene have been identified in bacteria. Here, we report the characterization of a locus where a small RNA (SymR) is encoded in cis to an SOS-induced gene whose product shows homology to the antitoxin MazE (SymE). Synthesis of the SymE protein is tightly repressed...

journal_title：Molecular microbiology

authors： Kawano M,Aravind L,Storz G

更新日期：2007-05-01 00:00:00

abstract：:The archaeal transcription apparatus is chimeric: its core components (RNA polymerase and basal factors) closely resemble those of eukaryotic RNA polymerase II, but the putative archaeal transcriptional regulators are overwhelmingly of bacterial type. Particular interest attaches to how these bacterial-type effectors,...

journal_title：Molecular microbiology

authors： Ouhammouch M,Langham GE,Hausner W,Simpson AJ,El-Sayed NM,Geiduschek EP

更新日期：2005-05-01 00:00:00

abstract：:Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...

journal_title：Molecular microbiology

authors： Cho Y,Kim KH,La Rota M,Scott D,Santopietro G,Callihan M,Mitchell TK,Lawrence CB

更新日期：2009-06-01 00:00:00

abstract：:Bacterial spores are surrounded by a multilayered proteinaceous shell called the coat. In Bacillus subtilis, a coat protein called CotE guides the assembly of a major subset of coat proteins. To understand how CotE carries out its role in coat morphogenesis, we subjected its gene to mutagenesis and studied the effects...

journal_title：Molecular microbiology

authors： Little S,Driks A

更新日期：2001-11-01 00:00:00

abstract：:Bacteria co-ordinate their social behaviour in a density-dependent manner by production of diffusible signal molecules by a process known as quorum sensing (QS). It is generally assumed that in homogenous environments and at high cell density, QS synchronizes cells in the population to perform collective social tasks ...

journal_title：Molecular microbiology

authors： Pradhan BB,Chatterjee S

更新日期：2014-05-01 00:00:00

abstract：:Listeria monocytogenes grows in the cytosol of mammalian cells and spreads from cell to cell without exiting the intracellular milieu. During cell-cell spread, bacteria become transiently entrapped in double-membrane vacuoles. Escape from these vacuoles is mediated in part by a bacterial phospholipase C (PC-PLC), whos...

journal_title：Molecular microbiology

authors： Marquis H,Hager EJ

更新日期：2000-01-01 00:00:00

abstract：:The Salmonella enterica serotype Typhimurium (S. typhimurium) genome contains a large repertoire of putative fimbrial operons that remain poorly characterized because they are not expressed in vitro. In this study, insertions that induced expression of the putative stdABCD fimbrial operon were identified from a random...

journal_title：Molecular microbiology

authors： Chessa D,Winter MG,Nuccio SP,Tükel C,Bäumler AJ

更新日期：2008-05-01 00:00:00