Abstract:
:We have isolated RNA polymerase from Mycobacterium smegmatis and established conditions for specific transcription initiation in vitro. The M. smegmatis enzyme has a strong dependence on supercoiling of the DNA substrate for transcription from mycobacterial promoters. We also show that RNA polymerase is the target for rifampicin, and that this antibiotic specifically inhibits the transition from synthesis of short oligoribonucleotides to full-length transcripts. RNA polymerase isolated from a rifampicin-resistant mutant of M. smegmatis is less sensitive to rifampicin in vitro, confirming that one mechanism of rifampicin resistance in mycobacteria is through alteration of RNA polymerase. This in vitro transcription system provides a simple method for the characterization of gene expression in mycobacteria including the pathogens Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium leprae. It also provides a system for evaluating potential anti-mycobacterial drugs.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Levin ME,Hatfull GFdoi
10.1111/j.1365-2958.1993.tb01572.xsubject
Has Abstractpub_date
1993-04-01 00:00:00pages
277-85issue
2eissn
0950-382Xissn
1365-2958journal_volume
8pub_type
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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