Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by alkaline phosphatase fusions.

abstract：:A system where archaeal gene expression could be controlled by simple manipulation of growth conditions would enable the construction of conditional lethal mutants in essential genes, and permit the controlled overproduction of proteins in their native host. As tools for the genetic manipulation of Haloferax volcanii ...

journal_title：Molecular microbiology

authors： Large A,Stamme C,Lange C,Duan Z,Allers T,Soppa J,Lund PA

更新日期：2007-12-01 00:00:00

abstract：:The volume of electron flow through the cbb3 branch of the electron transport chain and the redox state of the quinone pool generate signals that regulate photosynthesis gene expression in Rhodobacter sphaeroides. An inhibitory signal is generated at the level of the catalytic subunit of the cbb3 cytochrome c oxidase ...

journal_title：Molecular microbiology

authors： Oh JI,Kaplan S

更新日期：2001-03-01 00:00:00

abstract：:The minimum time (E) required for a new pair of replication origins (oriCs) produced upon initiating a round of replication to be ready to initiate the next round after one cell mass doubling, the 'eclipse', is explained in terms of a minimal distance (l(min)) that the replication forks must move away from oriC before...

journal_title：Molecular microbiology

authors： Zaritsky A,Vischer N,Rabinovitch A

更新日期：2007-01-01 00:00:00

abstract：:Stable RNA maturation is a key process in the generation of functional RNAs, and failure to correctly process these RNAs can lead to their elimination through quality control mechanisms. Studies of the maturation pathways of ribosomal RNA and transfer RNA in Bacillus subtilis showed they were radically different from ...

journal_title：Molecular microbiology

authors： Gilet L,DiChiara JM,Figaro S,Bechhofer DH,Condon C

更新日期：2015-01-01 00:00:00

abstract：:The genes ftsE and ftsX are organized in one operon together with ftsY. FtsY codes for the receptor of the signal recognition particle (SRP) that functions in targeting a subset of inner membrane proteins. We have found no indications for a structural relationship between FtsE/X and FtsY. Evidence is presented that Ft...

journal_title：Molecular microbiology

authors： de Leeuw E,Graham B,Phillips GJ,ten Hagen-Jongman CM,Oudega B,Luirink J

更新日期：1999-02-01 00:00:00

abstract：:We have used a targeted gene deletion event to remove the coding region for the bgl1 gene encoding an extracellular beta-glucosidase from the genome of the cellulolytic fungus Trichoderma reesei. The bgl1 null mutants were used to investigate the role of beta-glucosidase in the hydrolysis of cellulose and induction of...

journal_title：Molecular microbiology

authors： Fowler T,Brown RD Jr

更新日期：1992-11-01 00:00:00

abstract：:Entamoeba histolytica cells secrete electron-dense granules (EDGs) that have collagenase activity. To study the possible involvement of calmodulin (CaM) on EDG secretion, the effect of several CaM antagonists (TFP, R24571, W-7, W-5, dibucaine and DL-propranolol) was tested on this cellular function. Except for W-5 and...

journal_title：Molecular microbiology

authors： Muñoz Mde L,Moreno MA,Pérez-Garcia JN,Tovar GR,Hernandez VI

更新日期：1991-07-01 00:00:00

abstract：:Escherichia coli haemolysin (Hlya) is a pore-forming protein which belongs to the family of 'Repeat-toxins' (RTX) (Lo et al., 1987; Lally et al., 1989; Kraig et al., 1990). A model for the pore-forming structure of HlyA has been proposed (Ludwig et al., 1991) which consists of eight transmembrane segments all present ...

journal_title：Molecular microbiology

authors： Oropeza-Wekerle RL,Muller S,Briand JP,Benz R,Schmid A,Goebel W

更新日期：1992-01-01 00:00:00

abstract：:In prokaryotes, the principal signal transduction systems operating at the level of protein phosphorylation are the two-component systems. A number of hybrid histidine protein kinases in these systems contain several receiver domains, however, the function of these receiver domains is unknown. The RodK kinase in Myxoc...

journal_title：Molecular microbiology

authors： Rasmussen AA,Wegener-Feldbrügge S,Porter SL,Armitage JP,Søgaard-Andersen L

更新日期：2006-04-01 00:00:00

abstract：:The cloned Citrobacter freundii ampC beta-lactamase is inducible in the presence of its regulatory gene ampR in Escherichia coli (Lindberg et al., 1985). The basal level of expression and inducibility are affected by two E. coli proteins encoded by the closely linked ampD and ampE genes. Deletion of both genes led to ...

journal_title：Molecular microbiology

authors： Lindquist S,Galleni M,Lindberg F,Normark S

更新日期：1989-08-01 00:00:00

abstract：:Linear plasmids and chromosomes of Streptomyces carry terminal proteins (TPs) covalently attached to the 5' ends of the DNA. Most known telomeres are conserved in primary sequence and in the potential secondary structures formed during replication. The TP that caps these telomeres is also highly conserved and its codi...

journal_title：Molecular microbiology

authors： Huang CH,Tsai HH,Tsay YG,Chien YN,Wang SL,Cheng MY,Ke CH,Chen CW

更新日期：2007-03-01 00:00:00

abstract：:Phospholipid homeostasis of the bacterial membrane is maintained by biochemical regulation of the synthesis enzymes depending on the environment. However, genes encoding phospholipid synthesis enzymes might also be regulated during stress responses, in order for the bacteria to adapt their growth to changing environme...

journal_title：Molecular microbiology

authors： Wahl A,My L,Dumoulin R,Sturgis JN,Bouveret E

更新日期：2011-06-01 00:00:00

abstract：:Microtubules are key players in the biology of Trypanosomatid parasites, not only as classical components of the mitotic spindle, microtubule-organizing centres and flagellum but also as the essential constituent of the cytoskeleton. Their length dynamics are regulated by, among others, microtubule-severing proteins. ...

journal_title：Molecular microbiology

authors： Casanova M,Crobu L,Blaineau C,Bourgeois N,Bastien P,Pagès M

更新日期：2009-03-01 00:00:00

abstract：:Colonization of mucosal respiratory surfaces is a prerequisite for the human pathobiont Streptococcus pneumoniae (the pneumococcus) to cause severe invasive infections. The arsenal of pneumococcal adhesins interacts with a multitude of extracellular matrix proteins. A paradigm for pneumococci is their interaction with...

journal_title：Molecular microbiology

authors： Kanwal S,Jensch I,Palm GJ,Brönstrup M,Rohde M,Kohler TP,Somplatzki D,Tegge W,Jenkinson HF,Hammerschmidt S

更新日期：2017-09-01 00:00:00

abstract：:The overall transcription of DNA has previously been demonstrated to proceed at extremely high levels of accuracy. We review the evidence that the process of transcription is subject to proof-reading in the Hopfield sense. In addition, we speculate that the proof-reading activity associated with transcription is subje...

journal_title：Molecular microbiology

authors： Libby RT,Gallant JA

更新日期：1991-05-01 00:00:00

abstract：:Virulence factors secreted by Listeria monocytogenes are known to interfere with host cellular signalling pathways. We investigated whether L. monocytogenes modulates T-cell receptor signalling by examining surface expression of proteins known to be upregulated on activated T cells. In vitro culture of murine splenocy...

journal_title：Molecular microbiology

authors： Zenewicz LA,Skinner JA,Goldfine H,Shen H

更新日期：2004-03-01 00:00:00

abstract：:Two-component signal transduction (TCST) pathways are regulatory systems that are highly homologous throughout the bacterial kingdom. Their established role in virulence and absence in vertebrates has made TCST an attractive target for therapeutic intervention. However, such systems have yet to yield success in the de...

journal_title：Molecular microbiology

authors： Hubbard JA,MacLachlan LK,King GW,Jones JJ,Fosberry AP

更新日期：2003-09-01 00:00:00

abstract：:The RusA protein of Escherichia coli is a DNA structure-specific endonuclease that resolves Holliday junction intermediates formed during DNA replication, recombination and repair by introducing symmetrically paired incisions 5' to CC dinucleotides. It is encoded by the defective prophage DLP12, which raises the possi...

journal_title：Molecular microbiology

authors： Sharples GJ,Bolt EL,Lloyd RG

更新日期：2002-04-01 00:00:00

abstract：:The fitness of host-associated microbes depends on their ability to access nutrients in vivo. Identifying these mechanisms is significant for understanding how microbes have evolved to fill specific ecological niches within a host. Vibrio fischeri is a bioluminescent bacterium that colonizes and proliferates within th...

journal_title：Molecular microbiology

authors： Wasilko NP,Larios-Valencia J,Steingard CH,Nunez BM,Verma SC,Miyashiro T

更新日期：2019-03-01 00:00:00

abstract：:The largest subunit of RNA polymerase II, Rpb1, contains an unusual C-terminal domain (CTD) composed of numerous repeats of the YSPTSPS consensus sequence. This sequence is the target of post-translational modifications such as phosphorylation, glycosylation, methylation and transitions between stereoisomeric states, ...

journal_title：Molecular microbiology

authors： Drogat J,Hermand D

更新日期：2012-06-01 00:00:00

abstract：:Natural genetic transformation in Streptococcus pneumoniae is controlled in part by a quorum-sensing system mediated by a peptide pheromone called competence-stimulating peptide (CSP), which acts to coordinate transient activation of genes required for competence. To characterize the transcriptional response and regul...

journal_title：Molecular microbiology

authors： Peterson SN,Sung CK,Cline R,Desai BV,Snesrud EC,Luo P,Walling J,Li H,Mintz M,Tsegaye G,Burr PC,Do Y,Ahn S,Gilbert J,Fleischmann RD,Morrison DA

更新日期：2004-02-01 00:00:00

abstract：:A 25 kbp DNA fragment from the chromosome of Rhodobacter capsulatus B10 carrying hydrogenase (hup) determinants was completely sequenced. Coding regions corresponding to 20 open reading frames were identified. The R. capsulatus hydrogenase-specific gene (hup and hyp) products bear significant structural identity to hy...

journal_title：Molecular microbiology

authors： Colbeau A,Richaud P,Toussaint B,Caballero FJ,Elster C,Delphin C,Smith RL,Chabert J,Vignais PM

更新日期：1993-04-01 00:00:00

abstract：:By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open readin...

journal_title：Molecular microbiology

authors： Torres L,Martín H,García-Saez MI,Arroyo J,Molina M,Sánchez M,Nombela C

更新日期：1991-11-01 00:00:00

abstract：:The pathogenicity of Plasmodium falciparum is partly due to parasite-induced host cell modifications. These modifications are facilitated by exported P. falciparum proteins, collectively referred to as the exportome. Export of several hundred proteins is mediated by the PEXEL/HT, a protease cleavage site. The PEXEL/HT...

journal_title：Molecular microbiology

authors： Schulze J,Kwiatkowski M,Borner J,Schlüter H,Bruchhaus I,Burmester T,Spielmann T,Pick C

更新日期：2015-07-01 00:00:00

abstract：:The Streptomyces wild-type chromosome is linear in all examples studied. The ends of the chromosome or telomeres consist of terminal inverted repeats of various sizes with proteins covalently bound to their 5' ends. The chromosome is very unstable and undergoes very large deletions spontaneously at rates higher than 0...

journal_title：Molecular microbiology

authors： Volff JN,Altenbuchner J

更新日期：1998-01-01 00:00:00

abstract：:Natural competence in Streptococcus pneumoniae is regulated by a quorum-sensing mechanism consisting of a competence-stimulating peptide (CSP), its dedicated secretion apparatus (ComAB), its histidine kinase receptor (ComD) and a response regulator (ComE). In this report, we show that ComE is a DNA-binding protein tha...

journal_title：Molecular microbiology

authors： Ween O,Gaustad P,Håvarstein LS

更新日期：1999-08-01 00:00:00

abstract：:DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular dom...

journal_title：Molecular microbiology

authors： Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

更新日期：2005-01-01 00:00:00

abstract：:Chlamydiae are bacterial parasites that carry out a distinct developmental cycle within host cells; however, the mechanisms by which these organisms regulate stage-specific gene expression are not known. We identified a DNA element located between nucleotide (nt) -135 and -90 upstream from the transcription start poin...

journal_title：Molecular microbiology

authors： Zhong J,Douglas AL,Hatch TP

更新日期：2001-07-01 00:00:00

abstract：:Helicobacter pylori persists deep in the human gastric mucus layer in a harsh, nutrient-poor environment. Survival under these conditions depends on the ability of this human pathogen to invoke starvation/stress responses when needed. Unlike many bacteria, H. pylori lacks starvation/stress-responding alternative sigma...

journal_title：Molecular microbiology

authors： Yang ZX,Zhou YN,Yang Y,Jin DJ

更新日期：2010-08-01 00:00:00

abstract：:The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...

journal_title：Molecular microbiology

authors： Delany I,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-12-01 00:00:00