Abstract:
:The overall transcription of DNA has previously been demonstrated to proceed at extremely high levels of accuracy. We review the evidence that the process of transcription is subject to proof-reading in the Hopfield sense. In addition, we speculate that the proof-reading activity associated with transcription is subject to cyclical phase transitions. That is, during periods of low processivity associated with initiation, RNA synthesis is relatively imprecise. The transition to the elongation phase of RNA synthesis, characterized by a shift to high processivity, is accompanied by enhanced proof-reading. A model for the damping of transcriptional errors, based on a PPi-mediated processive pyrophosphorolysis reaction, is discussed in terms of pausing during transcription.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Libby RT,Gallant JAdoi
10.1111/j.1365-2958.1991.tb01872.xsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
999-1004issue
5eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
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