An amino-terminal threonine/serine motif is necessary for activity of the Crp/Fnr homolog, MrpC and for Myxococcus xanthus developmental robustness.

abstract：:The expression of dnaA is autoregulated, in that transcription of the gene increases when DnaA is inactivated (and initiation of replication prevented) and decreases when DnaA is supplied in excess. However, the inactivation of DnaA does not necessarily lead to increased DnaA production, as dnaA(Ts; temperature sensit...

journal_title：Molecular microbiology

authors： Smith RW,McAteer S,Masters M

更新日期：1997-03-01 00:00:00

abstract：:The actin-like protein FtsA is present in many eubacteria, and genetic experiments have shown that it plays an important, sometimes essential, role in cell division. Here, we show that Bacillus subtilis FtsA is targeted to division sites in both vegetative and sporulating cells. As in other organisms FtsA is probably ...

journal_title：Molecular microbiology

authors： Feucht A,Lucet I,Yudkin MD,Errington J

更新日期：2001-04-01 00:00:00

abstract：:Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here,...

journal_title：Molecular microbiology

authors： Davis R,Écija-Conesa A,Gallego-Jara J,de Diego T,Filippova EV,Kuffel G,Anderson WF,Gibson BW,Schilling B,Canovas M,Wolfe AJ

更新日期：2018-01-01 00:00:00

abstract：:A number of small, multicopy plasmids from Gram-positive bacteria replicate by an asymmetric rolling-circle mechanism. Previous studies with several of these plasmids have identified a palindromic sequence, SSOA, that acts as the single-strand origin (SSO) for the replication of the lagging-strand DNA. Although not al...

journal_title：Molecular microbiology

authors： Dempsey LA,Zhao AC,Khan SA

更新日期：1995-02-01 00:00:00

abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...

journal_title：Molecular microbiology

authors： Ramseier TM,Bledig S,Michotey V,Feghali R,Saier MH Jr

更新日期：1995-06-01 00:00:00

abstract：:Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...

journal_title：Molecular microbiology

authors： Shakhnovich EA,Sturtevant D,Mekalanos JJ

更新日期：2007-12-01 00:00:00

abstract：:Replacement of the amino-terminal 40-amino-acid region of the 588-amino-acid precursor of the membrane-bound penicillin-binding protein 3 (PBP3) by the decapeptide MKGKEFQAWI was carried out by altering the amino-coding end of the ftsI gene. Insertion of the modified gene into a runaway-replication plasmid under the c...

journal_title：Molecular microbiology

authors： Belder JB,Nguyen-Distèche M,Houba-Herin N,Ghuysen JM,Maruyama IN,Hara H,Hirota Y,Inouye M

更新日期：1988-07-01 00:00:00

abstract：:The stable RNA promoters of Escherichia coli are exquisitely sensitive to variations in the superhelical density of DNA. Previously, we have shown that binding of the DNA architectural protein FIS at the upstream activating sequences (UASs) of stable RNA promoters prevents the transcription complexes from inactivation...

journal_title：Molecular microbiology

authors： Rochman M,Blot N,Dyachenko M,Glaser G,Travers A,Muskhelishvili G

更新日期：2004-07-01 00:00:00

abstract：:Sinorhizobium meliloti DctB is a typical transmembrane sensory histidine kinase, which senses C(4)-dicarboxylic acids (DCA) and regulates the expression of DctA, the DCA transporter. We previously reported the crystal structures of its periplasmic sensory domain (DctBp) in apo and succinate-bound states, and these str...

journal_title：Molecular microbiology

authors： Nan B,Liu X,Zhou Y,Liu J,Zhang L,Wen J,Zhang X,Su XD,Wang YP

更新日期：2010-03-01 00:00:00

abstract：:Slipped-strand mispairing at sites containing so-called coding repeats (CRs) can lead to phase variation of surface proteins in Gram-negative bacteria. This mechanism, believed to contribute to virulence, has so far not been identified in a Gram-positive bacterium. In the genome of the Gram-positive human pathogen Str...

journal_title：Molecular microbiology

authors： Rasmussen M,Björck L

更新日期：2001-06-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:Many types of microorganisms, including both prokaryotes and eukaryotes, have developed mechanisms to adapt to severe osmotic stress. In this study, we isolated multicopy suppressor genes for a Schizosaccharomyces pombe mutant, which exhibited the clear phenotype of being osmosensitive for growth (Osms) on agar plates...

journal_title：Molecular microbiology

authors： Ohmiya R,Yamada H,Nakashima K,Aiba H,Mizuno T

更新日期：1995-12-01 00:00:00

abstract：:Escherichia coli FtsN is a bitopic membrane protein that is essential for triggering active cell constriction. A small periplasmic subdomain ((E) FtsN) is required and sufficient for function, but its mechanism of action is unclear. We isolated extragenic (E) FtsN*-suppressing mutations that restore division in cells ...

journal_title：Molecular microbiology

authors： Liu B,Persons L,Lee L,de Boer PA

更新日期：2015-03-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00

abstract：:The Yersinia Ysc apparatus is made of more than 20 proteins, 11 of which have homologues in many type III systems. Here, we characterize YscP from Yersinia enterocolitica. This 515-residue protein has a high proline content, a large tandem repetition and a slow migration in SDS-PAGE. Unlike the products of neighbourin...

journal_title：Molecular microbiology

authors： Stainier I,Bleves S,Josenhans C,Karmani L,Kerbourch C,Lambermont I,Tötemeyer S,Boyd A,Cornelis GR

更新日期：2000-09-01 00:00:00

abstract：:The mechanistic details of the pathogenesis of Chlamydia, an obligate intracellular pathogen of global importance, have eluded scientists due to the scarcity of traditional molecular genetic tools to investigate this organism. Here we report a chemical biology strategy that has uncovered the first essential protease f...

journal_title：Molecular microbiology

authors： Gloeckl S,Ong VA,Patel P,Tyndall JD,Timms P,Beagley KW,Allan JA,Armitage CW,Turnbull L,Whitchurch CB,Merdanovic M,Ehrmann M,Powers JC,Oleksyszyn J,Verdoes M,Bogyo M,Huston WM

更新日期：2013-08-01 00:00:00

abstract：:A region of 7.8 kb of the plasmid pMB2 from Enterococcus faecalis S-48 carrying the information necessary for production and immunity of the peptide antibiotic AS-48 has been cloned and sequenced. It contains the as-48A structural gene plus five open reading frames (as-48B, as-48C, as-48C1, as-48D and as-48D1). Beside...

journal_title：Molecular microbiology

authors： Martínez-Bueno M,Valdivia E,Gálvez A,Coyette J,Maqueda M

更新日期：1998-01-01 00:00:00

abstract：:Restriction and modification (R-M) systems are generally thought to protect bacteria from invasion by foreign DNA. This paper proposes the existence of an alternative role for the phase-variable R-M systems encoded by the hsd loci of Mycoplasma pulmonis. Populations of M. pulmonis cells that arose during growth in dif...

journal_title：Molecular microbiology

authors： Gumulak-Smith J,Teachman A,Tu AH,Simecka JW,Lindsey JR,Dybvig K

更新日期：2001-05-01 00:00:00

abstract：:Mycoplasma agalactiae, an important pathogen of small ruminants, exhibits antigenic diversity by switching the expression of multiple surface lipoproteins called Vpmas (Variable proteins of M. agalactiae). Although phase variation has been shown to play important roles in many host-pathogen interactions, the biologica...

journal_title：Molecular microbiology

authors： Chopra-Dewasthaly R,Citti C,Glew MD,Zimmermann M,Rosengarten R,Jechlinger W

更新日期：2008-03-01 00:00:00

abstract：:The mechanisms of transcription repression and derepression in vivo are not fully understood. We have obtained evidence that begins to clarify the minimum requirements for counteracting nucleosomal repression in vivo. Location of the TATA element near the nucleosome dyad does not block RNA polymerase II transcription ...

journal_title：Molecular microbiology

authors： Moss DR,Laybourn PJ

更新日期：2000-06-01 00:00:00

abstract：:Pseudomonas aeruginosa OprD is a 420-amino-acid protein that facilitates the uptake of basic amino acids, imipenem and gluconate across the outer membrane. OprD was the first specific porin that could be aligned with members of the non-specific porin super-family. Utilizing multiple alignments in conjugation with stru...

journal_title：Molecular microbiology

authors： Huang H,Jeanteur D,Pattus F,Hancock RE

更新日期：1995-06-01 00:00:00

abstract：:Penicillium marneffei is a thermally dimorphic opportunistic human pathogen with a saprophytic filamentous hyphal form at 25 degrees C and a pathogenic unicellular yeast form at 37 degrees C. During infection. P. marneffei yeast cells exist intracellularly in macrophages. To cope with nutrient deprivation during the i...

journal_title：Molecular microbiology

authors： Cánovas D,Andrianopoulos A

更新日期：2006-12-01 00:00:00

abstract：:The glycopeptide antibiotic-producing bacterium, Streptomyces toyocaensis NRRL 15009, has proteins phosphorylated on Ser, Thr, Tyr and His, implying the presence of a battery of associated kinases. We have identified the Ser/Thr protein kinase gene fragments stoPK-1, stoPK-2, stoPK-3 and stoPK-4 from S. toyocaensis NR...

journal_title：Molecular microbiology

authors： Neu JM,MacMillan SV,Nodwell JR,Wright GD

更新日期：2002-04-01 00:00:00

abstract：:Microcins are a class of ribosomally synthesized antibacterial peptides produced by Enterobacteriaceae and active against closely related bacterial species. While some microcins are active as unmodified peptides, others are heavily modified by dedicated maturation enzymes. Low-molecular-weight microcins from the post-...

journal_title：Molecular microbiology

authors： Severinov K,Semenova E,Kazakov A,Kazakov T,Gelfand MS

更新日期：2007-09-01 00:00:00

abstract：:Capsular polysaccharides are well-established virulence factors of pathogenic bacteria. Their biosynthesis and export are regulated within the transmembrane polysaccharide assembly machinery by the autophosphorylation of atypical tyrosine-kinases, named BY-kinases. However, the accurate functioning of these tyrosine-k...

journal_title：Molecular microbiology

authors： Bechet E,Gruszczyk J,Terreux R,Gueguen-Chaignon V,Vigouroux A,Obadia B,Cozzone AJ,Nessler S,Grangeasse C

更新日期：2010-09-01 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00

abstract：:We previously characterized a defective-folding mutant of maltose-binding protein of Escherichia coli, MalE31, which formed periplasmic inclusion bodies. Here, we show that MalE31 aggregation does not affect bacterial growth at 30 degrees C but is lethal at 37 degrees C. Surprisingly, under mild heat shock conditions ...

journal_title：Molecular microbiology

authors： Hunke S,Betton JM

更新日期：2003-12-01 00:00:00

abstract：:Streptococcus lactis NCDO763 harbours a plasmid designated pLP763. The cells harbouring pLP763 are able to grow to a higher density in milk because of their proteinase-positive phenotype (Prt+). The 6.2 kb HindIII-PstI fragment from pLP763 was found to be responsible for the Prt+ phenotype. The DNA fragment contains a...

journal_title：Molecular microbiology

authors： Kiwaki M,Ikemura H,Shimizu-Kadota M,Hirashima A

更新日期：1989-03-01 00:00:00

abstract：:The stringent response is important for bacterial survival under stressful conditions, such as amino acid starvation, and is characterized by the accumulation of ppGpp and pppGpp. ObgE (CgtA, YhbZ) is an essential conserved GTPase in Escherichia coli and several observations have implicated the protein in the control ...

journal_title：Molecular microbiology

authors： Persky NS,Ferullo DJ,Cooper DL,Moore HR,Lovett ST

更新日期：2009-07-01 00:00:00

abstract：:The Plasmodium falciparum genome sequence has boosted hopes for a new era of malaria research and for the application of comprehensive molecular knowledge to disease control, but formidable obstacles remain: approximately 60% of the predicted P. falciparum proteins have no known functions or homologues, and most life ...

journal_title：Molecular microbiology

authors： Su XZ,Wootton JC

更新日期：2004-09-01 00:00:00