Identification of a serine protease inhibitor which causes inclusion vacuole reduction and is lethal to Chlamydia trachomatis.

abstract：:Whereas in Bacillus subtilis, a general stress response stimulon under the control of a single sigma factor (SigB) is induced by different physiological and environmental stresses (heat, salt or ethanol shock), in Streptomyces coelicolor, these environmental stresses induce independent sets of proteins, and its genome...

journal_title：Molecular microbiology

authors： Viollier PH,Kelemen GH,Dale GE,Nguyen KT,Buttner MJ,Thompson CJ

更新日期：2003-02-01 00:00:00

abstract：:Previous studies have shown that in Saccharomyces cerevisiae the mitochondrial and cytoplasmic forms of alanyl-tRNA synthetase are encoded by a single nuclear gene, ALA1, through alternative use of in-frame successive ACG triplets and a downstream AUG triplet. Here we show that despite the obvious participation of the...

journal_title：Molecular microbiology

authors： Huang HY,Tang HL,Chao HY,Yeh LS,Wang CC

更新日期：2006-04-01 00:00:00

abstract：:The expression of the structural genes for lysine (LYS) biosynthesis is controlled by a pathway-specific regulation mediated by the transcriptional activator Lys14 in the presence of alpha-aminoadipate semialdehyde, an intermediate of the pathway acting as a co-inducer. Owing to end product inhibition of the first ste...

journal_title：Molecular microbiology

authors： Becker B,Feller A,el Alami M,Dubois E,Piérard A

更新日期：1998-07-01 00:00:00

abstract：:The effect of the unc transcription terminator on expression of uncC, encoding the epsilon subunit of Escherichia coli F1-ATPase, from plasmids was studied. The cloned sequence in pTK1 included the uncC ribosome binding site, the uncC structural gene, and the unc transcription terminator. The cloned region in pSD37 wa...

journal_title：Molecular microbiology

authors： Patel AM,Dallmann HG,Skakoon EN,Kapala TD,Dunn SD

更新日期：1990-11-01 00:00:00

abstract：:Factors that enhance the transmission of pathogens are poorly understood. We show that Vibrio cholerae shed in human 'rice-water' stools have a 10-fold lower oral infectious dose in an animal model than in vitro grown V. cholerae, which may aid in transmission during outbreaks. Furthermore, we identify a bacterial fac...

journal_title：Molecular microbiology

authors： Butler SM,Nelson EJ,Chowdhury N,Faruque SM,Calderwood SB,Camilli A

更新日期：2006-04-01 00:00:00

abstract：:Site-specific recombination by the Cre recombinase takes place at a simple DNA site (loxP), requires no additional proteins and gives topologically simple recombination products. In contrast, cer and psi sites for Xer recombination contain approximately 150 bp of accessory sequences, require accessory proteins PepA, A...

journal_title：Molecular microbiology

authors： Gourlay SC,Colloms SD

更新日期：2004-04-01 00:00:00

abstract：:The pulmonary pathogen Legionella pneumophila uses the Dot/Icm type IV secretion system (T4SS) to replicate inside host cells. This apparatus translocates proteins into macrophages to alter their endocytic pathway and enable bacterial growth. Although the secretion ATPase DotB is critical for T4SS function, its specif...

journal_title：Molecular microbiology

authors： Sexton JA,Yeo HJ,Vogel JP

更新日期：2005-07-01 00:00:00

abstract：:Deletions or substitutions of amino acids at the carboxyl-terminus of the heat-labile enterotoxin B subunit (EtxB) affect its assembly into pentamers in a temperature-dependent manner. At 42 degrees C, the mutations prevent the B subunits from achieving their final pentameric structure resulting in membrane associatio...

journal_title：Molecular microbiology

authors： Sandkvist M,Bagdasarian M

更新日期：1993-11-01 00:00:00

abstract：:Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (sigma) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cau...

journal_title：Molecular microbiology

authors： Manganelli R,Voskuil MI,Schoolnik GK,Dubnau E,Gomez M,Smith I

更新日期：2002-07-01 00:00:00

abstract：:A new locus required for type 4 pilus biogenesis by Pseudomonas aeruginosa has been identified. A pilE mutant, designated MJ-6, was broadly resistant to pili-specific phages and unable to translocate across solid surfaces by the pilus-dependent mechanism of twitching motility (Twt-). Immunoblot analysis demonstrated t...

journal_title：Molecular microbiology

authors： Russell MA,Darzins A

更新日期：1994-09-01 00:00:00

abstract：:The Bacillus subtilis ssuBACD gene cluster is required for the utilization of aliphatic sulphonates as sulphur sources. The regulation of expression of the ssu genes was studied in constructs carrying chromosomal transcriptional fusions of the ssu promoter region to lacZ. When sulphate or cystine served as sulphur sou...

journal_title：Molecular microbiology

authors： van der Ploeg JR,Barone M,Leisinger T

更新日期：2001-03-01 00:00:00

abstract：:Sinorhizobium meliloti DctB is a typical transmembrane sensory histidine kinase, which senses C(4)-dicarboxylic acids (DCA) and regulates the expression of DctA, the DCA transporter. We previously reported the crystal structures of its periplasmic sensory domain (DctBp) in apo and succinate-bound states, and these str...

journal_title：Molecular microbiology

authors： Nan B,Liu X,Zhou Y,Liu J,Zhang L,Wen J,Zhang X,Su XD,Wang YP

更新日期：2010-03-01 00:00:00

abstract：:Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity. Both Fis and H-NS selectively repress the dps pro...

journal_title：Molecular microbiology

authors： Grainger DC,Goldberg MD,Lee DJ,Busby SJ

更新日期：2008-06-01 00:00:00

abstract：:In this study, we followed the genomic, lipidomic and metabolomic changes associated with the selection of miltefosine (MIL) resistance in two clinically derived Leishmania donovani strains with different inherent resistance to antimonial drugs (antimony sensitive strain Sb-S; and antimony resistant Sb-R). MIL-R was e...

journal_title：Molecular microbiology

authors： Shaw CD,Lonchamp J,Downing T,Imamura H,Freeman TM,Cotton JA,Sanders M,Blackburn G,Dujardin JC,Rijal S,Khanal B,Illingworth CJ,Coombs GH,Carter KC

更新日期：2016-03-01 00:00:00

abstract：:Cell-cell communication via the production and detection of chemical signal molecules has been the focus of a great deal of research over the past decade. One class of chemical signals widely used by proteobacteria consists of N-acyl-homoserine lactones, which are synthesized by proteins related to LuxI of Vibrio fisc...

journal_title：Molecular microbiology

authors： Pappas KM,Weingart CL,Winans SC

更新日期：2004-08-01 00:00:00

abstract：:The human oral bacterium Streptococcus gordonii expresses, on the cell surface, two antigenically related high-molecular-mass polypeptides denoted CshA and CshB, encoded by genes at separate chromosomal loci. The precursor form of CshA is composed of four distinct segments: (i) a 41-amino-acid residue leader peptide, ...

journal_title：Molecular microbiology

authors： McNab R,Jenkinson HF,Loach DM,Tannock GW

更新日期：1994-11-01 00:00:00

abstract：:Expression of the 987P gene cluster is activated by the adjacent IS1 element of an STpa transposon. Nucleotide sequence analysis of the 987P-DNA region contiguous with this IS1 element revealed the presence of an open reading frame designated fapR, encoding a basic protein of 260 amino acid residues with a molecular m...

journal_title：Molecular microbiology

authors： Klaasen P,de Graaf FK

更新日期：1990-10-01 00:00:00

abstract：:While examining the molecular basis for the lack of actin-based motility for the non-pathogenic spotted fever group (SFG) R. peacockii, we identified a novel insertion sequence (IS) element, ISRpe1, disrupting the coding sequence of rickA, demonstrated to induce actin-tail polymerization for the SFG rickettsiae. This ...

journal_title：Molecular microbiology

authors： Simser JA,Rahman MS,Dreher-Lesnick SM,Azad AF

更新日期：2005-10-01 00:00:00

abstract：:The segmented genome of Borrelia burgdorferi, a causative agent of Lyme disease, contains a mixture of over 20 linear and circular plasmids. Genes encoding five paralogous families of plasmid replication proteins are located on both circular and linear DNA molecules. The effect of DNA topology on the transcription of ...

journal_title：Molecular microbiology

authors： Beaurepaire C,Chaconas G

更新日期：2007-01-01 00:00:00

abstract：:A bioassay was developed to identify stimuli that promote the transcriptional induction of the algD operon for alginate biosynthesis in Pseudomonas aeruginosa. Strain PAO1 carried the algD promoter fused to a chloramphenicol acetyl-transferase cartridge (PalgD-cat), and > 50 compounds were tested for promoting chloram...

journal_title：Molecular microbiology

authors： Wood LF,Leech AJ,Ohman DE

更新日期：2006-10-01 00:00:00

abstract：:Full virulence of Yersinia enterocolitica Biovar 1B requires two distinct and distantly related contact-dependent type III secretion (T3S) systems. The plasmid-encoded Ysc T3S system is essential for systemic stages of infection and the Yop effector proteins it translocates have been extensively studied. The chromosom...

journal_title：Molecular microbiology

authors： Matsumoto H,Young GM

更新日期：2006-01-01 00:00:00

abstract：:Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...

journal_title：Molecular microbiology

authors： Shakhnovich EA,Sturtevant D,Mekalanos JJ

更新日期：2007-12-01 00:00:00

abstract：:In Escherichia coli the flagellar regulon consists of more than 60 genes organized in three hierarchically and temporally regulated transcriptional classes. The flagellar sigma factor FliA (sigma(28)) is responsible for class 3 expression and, in the early phase of flagellar assembly, is inhibited by its anti-sigma fa...

journal_title：Molecular microbiology

authors： Barembruch C,Hengge R

更新日期：2007-07-01 00:00:00

abstract：:Eeyarestatin 1 (ES1) is an inhibitor of endoplasmic reticulum (ER) associated protein degradation, Sec61-dependent Ca2+ homeostasis and protein translocation into the ER. Recently, evidence was presented showing that a smaller analog of ES1, ES24, targets the Sec61-translocon, and captures it in an open conformation t...

journal_title：Molecular microbiology

authors： Steenhuis M,Koningstein GM,Oswald J,Pick T,O'Keefe S,Koch HG,Cavalié A,Whitehead RC,Swanton E,High S,Luirink J

更新日期：2020-08-15 00:00:00

abstract：:The cloned Citrobacter freundii ampC beta-lactamase is inducible in the presence of its regulatory gene ampR in Escherichia coli (Lindberg et al., 1985). The basal level of expression and inducibility are affected by two E. coli proteins encoded by the closely linked ampD and ampE genes. Deletion of both genes led to ...

journal_title：Molecular microbiology

authors： Lindquist S,Galleni M,Lindberg F,Normark S

更新日期：1989-08-01 00:00:00

abstract：:We have found a simple relationship between bacterial growth rate and the translation elongation rate. Thus, for a set of defined ribosomal protein S12 mutations which reduce the efficiency of the ternary complex ribosome interaction (and restrict the frequency of translational errors) there is a linear relationship b...

journal_title：Molecular microbiology

authors： Tubulekas I,Hughes D

更新日期：1993-01-01 00:00:00

abstract：:A recurring theme in morphogenesis is the coupling of the expression of genes that drive morphogenesis and the morphogenetic process per se. This coupling ensures that gene expression and morphogenesis are carried out in synchrony. Morphogenesis of the spore-filled fruiting bodies in Myxococcus xanthus illustrates thi...

journal_title：Molecular microbiology

authors： Søgaard-Andersen L,Overgaard M,Lobedanz S,Ellehauge E,Jelsbak L,Rasmussen AA

更新日期：2003-04-01 00:00:00

abstract：:Urinary tract infection (UTI) is a very common extraintestinal infection, and Escherichia coli is by far the most common causative organism. Uropathogenic E. coli possess traits that distinguish them from commensal strains of E. coli, such as secretion systems that allow virulence factors to be targeted to extracytopl...

journal_title：Molecular microbiology

authors： Guyer DM,Henderson IR,Nataro JP,Mobley HL

更新日期：2000-10-01 00:00:00

abstract：:Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation ...

journal_title：Molecular microbiology

authors： Dempsey WB,Fee BE

更新日期：1990-06-01 00:00:00

abstract：:Candida albicans is the single, most frequently isolated human fungal pathogen. As with most fungal pathogens, the factors which contribute to pathogenesis in C. albicans are not known, despite more than a decade of molecular genetic analysis. Candida albicans was thought to be asexual until the discovery of the MTL l...

journal_title：Molecular microbiology

authors： Magee BB,Legrand M,Alarco AM,Raymond M,Magee PT

更新日期：2002-12-01 00:00:00