Buffering of stable RNA promoter activity against DNA relaxation requires a far upstream sequence.

abstract：:The ndh gene of Escherichia coli which encodes an NADH dehydrogenase contains a putative FNR-binding site in its upstream non-coding region, and its expression has been investigated using an ndh-lacZ fusion. Expression of the fusion was found to be reduced during anaerobic growth, and experiments with hosts containing...

journal_title：Molecular microbiology

authors： Spiro S,Roberts RE,Guest JR

更新日期：1989-05-01 00:00:00

abstract：:Among enteric bacteria, the ability to grow on N-acetyl-galactosamine (GalNAc or Aga) and on D-galactosamine (GalN or Gam) differs. Thus, strains B, C and EC3132 of Escherichia coli are Aga+ Gam+ whereas E. coli K-12 is Aga- Gam-, similarly to Klebsiella pneumoniae KAY2026, Klebsiella oxytoca M5a1 and Salmonella typhi...

journal_title：Molecular microbiology

authors： Brinkkötter A,Klöss H,Alpert C,Lengeler JW

更新日期：2000-07-01 00:00:00

abstract：:In contrast to most primary metabolism genes, the genes involved in secondary metabolism and certain nutrient utilization pathways are clustered in fungi. Recently a nuclear protein, LaeA, was found to be required for the transcription of several secondary metabolite gene clusters in Aspergillus nidulans. Here we show...

journal_title：Molecular microbiology

authors： Bok JW,Noordermeer D,Kale SP,Keller NP

更新日期：2006-09-01 00:00:00

abstract：:Renewed interest in gene amplification stems from its importance in evolution and a variety of medical problems ranging from drug resistance to cancer. However, amplified DNA segments (amplicons) are not fully characterized in any organism. Here we report a novel Acinetobacter baylyi system for genome-wide studies. Am...

journal_title：Molecular microbiology

authors： Seaton SC,Elliott KT,Cuff LE,Laniohan NS,Patel PR,Neidle EL

更新日期：2012-02-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00

abstract：:The CovR/S two-component system regulates the transcription of many genes that are crucial for the virulence of Streptococcus pyogenes (group A Streptococcus, GAS). Previously, we demonstrated that one gene repressed directly by CovR is rivR, which encodes a member of the RofA-like family of transcriptional regulators...

journal_title：Molecular microbiology

authors： Roberts SA,Scott JR

更新日期：2007-12-01 00:00:00

abstract：:The interactions between pathogenic bacteria and extracellular matrix (ECM) components markedly influence the initiation and establishment of infection. We have identified two surface proteins of virulent Mycoplasma pneumoniae with molecular masses of 45 and 30 kDa that bind to the ECM constituent, fibronectin (Fn). T...

journal_title：Molecular microbiology

authors： Dallo SF,Kannan TR,Blaylock MW,Baseman JB

更新日期：2002-11-01 00:00:00

abstract：:The volume of electron flow through the cbb3 branch of the electron transport chain and the redox state of the quinone pool generate signals that regulate photosynthesis gene expression in Rhodobacter sphaeroides. An inhibitory signal is generated at the level of the catalytic subunit of the cbb3 cytochrome c oxidase ...

journal_title：Molecular microbiology

authors： Oh JI,Kaplan S

更新日期：2001-03-01 00:00:00

abstract：:Regulation of gene expression in trypanosomatid parasitic protozoa is mainly achieved posttranscriptionally. RNA-binding proteins (RBPs) associate to 3' untranslated regions in mRNAs through dedicated domains such as the RNA recognition motif (RRM). Trypanosoma cruzi UBP1 (TcUBP1) is an RRM-type RBP involved in stabil...

journal_title：Molecular microbiology

authors： Cassola A,Romaniuk MA,Primrose D,Cervini G,D'Orso I,Frasch AC

更新日期：2015-09-01 00:00:00

abstract：:We show in this work that disruption of the hfq gene in Escherichia coli causes a large increase in IS10 transposition when IS10 is present on a multi-copy plasmid. Hfq is an RNA-binding protein that regulates the expression of a large number of genes at the post-transcriptional level by promoting the pairing of mRNAs...

journal_title：Molecular microbiology

authors： Ross JA,Wardle SJ,Haniford DB

更新日期：2010-11-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00

abstract：:The fungal pathogen Candida albicans can transition from budding to hyphal growth, which promotes biofilm formation and invasive growth into tissues. Stimulation of adenylyl cyclase to form cAMP induces hyphal morphogenesis. The failure of cells lacking adenylyl cyclase (cyr1Δ) to form hyphae has suggested that cAMP s...

journal_title：Molecular microbiology

authors： Parrino SM,Si H,Naseem S,Groudan K,Gardin J,Konopka JB

更新日期：2017-03-01 00:00:00

abstract：:MreB shares a common prokaryotic ancestor with actin and is present in almost all rod-shaped bacteria. MreB proteins have been implicated in a range of important cell processes, including cell morphogenesis, chromosome segregation and cell polarity. The mreB gene frequently lies at the beginning of a cluster of genes,...

journal_title：Molecular microbiology

authors： Formstone A,Errington J

更新日期：2005-03-01 00:00:00

abstract：:The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...

journal_title：Molecular microbiology

authors： Daubenspeck JM,Bolland JR,Luo W,Simmons WL,Dybvig K

更新日期：2009-06-01 00:00:00

abstract：:Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...

journal_title：Molecular microbiology

authors： Cho Y,Kim KH,La Rota M,Scott D,Santopietro G,Callihan M,Mitchell TK,Lawrence CB

更新日期：2009-06-01 00:00:00

abstract：:The involvement of signal transduction in the repair of radiation-induced damage to DNA has been known in eukaryotes but remains understudied in bacteria. This article for the first time demonstrates a role for the periplasmic lipoprotein (YfgL) with protein kinase activity transducing a signal for DNA strand break re...

journal_title：Molecular microbiology

authors： Khairnar NP,Kamble VA,Mangoli SH,Apte SK,Misra HS

更新日期：2007-07-01 00:00:00

abstract：:Cell-cell communication via the production and detection of chemical signal molecules has been the focus of a great deal of research over the past decade. One class of chemical signals widely used by proteobacteria consists of N-acyl-homoserine lactones, which are synthesized by proteins related to LuxI of Vibrio fisc...

journal_title：Molecular microbiology

authors： Pappas KM,Weingart CL,Winans SC

更新日期：2004-08-01 00:00:00

abstract：:Successful pathogens must be able to swiftly respond to and repair DNA damages inflicted by the host defence. The replication protein A (RPA) complex plays multiple roles in DNA damage response and is regulated by phosphorylation. However, the regulators of RPA phosphorylation remain unclear. Here, we investigated Rfa...

journal_title：Molecular microbiology

authors： Gao J,Wang H,Wong AH,Zeng G,Huang Z,Wang Y,Sang J,Wang Y

更新日期：2014-10-01 00:00:00

abstract：:Small repeat sequences in bacterial genomes, which represent non-autonomous mobile elements, have close similarities to archaeon and eukaryotic miniature inverted repeat transposable elements. These repeat elements are found in both intergenic and intragenic chromosomal regions, and contain an array of diverse motifs....

journal_title：Molecular microbiology

authors： Delihas N

更新日期：2008-02-01 00:00:00

abstract：:The Clostridium difficile toxA and toxB genes, encoding cytotoxic and enterotoxic proteins responsible for antibiotic-associated colitis and pseudomembranous colitis, were shown to be transcribed both from gene-specific promoters and from promoters of upstream genes. However, the gene-specific transcripts represented ...

journal_title：Molecular microbiology

authors： Dupuy B,Sonenshein AL

更新日期：1998-01-01 00:00:00

abstract：:Malaria transmission requires that the parasites differentiate into gametocytes prior to ingestion by a mosquito during a blood meal. Once in the mosquito midgut the gametocytes emerge from red blood cells (RBCs), fertilize, develop into ookinetes and finally infectious sporozoites. Gamete surface antigen, Pfs230, is ...

journal_title：Molecular microbiology

authors： Eksi S,Czesny B,van Gemert GJ,Sauerwein RW,Eling W,Williamson KC

更新日期：2006-08-01 00:00:00

abstract：:Comparison of the Leishmania infantum genome with Leishmania braziliensis and Leishmania major genomes has identified 25 L. infantum species-specific genes that are absent or pseudogenes in L. major and L. braziliensis. To determine whether these L. infantum species-specific genes are involved in visceral Leishmania i...

journal_title：Molecular microbiology

authors： Zhang WW,Matlashewski G

更新日期：2010-07-01 00:00:00

abstract：:Meningococcal gdhA, encoding the NADP-specific l-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promote...

journal_title：Molecular microbiology

authors： Pagliarulo C,Salvatore P,De Vitis LR,Colicchio R,Monaco C,Tredici M,Talà A,Bardaro M,Lavitola A,Bruni CB,Alifano P

更新日期：2004-03-01 00:00:00

abstract：:Oligosaccharides derived from cell wall of fungal pathogens induce host primary immune responses. To understand fungal strategies circumventing the host plant immune responses, cell wall polysaccharide localization was investigated using fluorescent labels during infectious structure differentiation in the rice blast ...

journal_title：Molecular microbiology

authors： Fujikawa T,Kuga Y,Yano S,Yoshimi A,Tachiki T,Abe K,Nishimura M

更新日期：2009-08-01 00:00:00

abstract：:Although once thought to be unique to bacteria, d-amino acids are also produced by mammals. For example, d-serine is excreted in human urine at concentrations ranging from 3.0 to 40 micro g ml-1. An epidemiological survey demonstrated that urine isolates of E. coli are more likely to catabolise d-serine via expression...

journal_title：Molecular microbiology

authors： Roesch PL,Redford P,Batchelet S,Moritz RL,Pellett S,Haugen BJ,Blattner FR,Welch RA

更新日期：2003-07-01 00:00:00

abstract：:Listeria monocytogenes FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the Bacillus subtilis Fur- and PerR-regulated Fe(II) efflux P1B4 -type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an frvA mutant is sensitive to iron int...

journal_title：Molecular microbiology

authors： Pi H,Patel SJ,Argüello JM,Helmann JD

更新日期：2016-06-01 00:00:00

abstract：:Summary Spore formation in the Gram-positive bacterium Bacillus subtilis is a last resort adaptive response to starvation. To initiate sporulation, the key regulator in this process, Spo0A, needs to be activated by the so-called phosphorelay. Within a sporulating culture of B. subtilis, some cells initiate this develo...

journal_title：Molecular microbiology

authors： Veening JW,Hamoen LW,Kuipers OP

更新日期：2005-06-01 00:00:00

abstract：:The genes of nicotine dehydrogenase (NDH) were identified, cloned and sequenced from the catabolic plasmid pAO1 of Arthrobacter nicotinovorans. In immediate proximity to this gene cluster is the beginning of the 6-hydroxy-L-niotine oxidase (6-HLNO) gene. NDH is composed of three subunits (A, B and C) of M(r) 30,011, 1...

journal_title：Molecular microbiology

authors： Grether-Beck S,Igloi GL,Pust S,Schilz E,Decker K,Brandsch R

更新日期：1994-09-01 00:00:00

abstract：:Transcription in vitro of two osmoregulated promoters, for the Escherichia coli osmB and osmY genes, was analysed using two species of RNA polymerase holoenzyme reconstituted from purified core enzyme and either sigma D (sigma 70, the major sigma in exponentially growing cells) or sigma S (sigma 38, the principal sigm...

journal_title：Molecular microbiology

authors： Ding Q,Kusano S,Villarejo M,Ishihama A

更新日期：1995-05-01 00:00:00

abstract：:Cell-cell and cell-surface adherence represents initial steps in forming multicellular aggregates or in establishing cell-surface interactions. The commonly used Saccharomyces cerevisiae laboratory strain S288c carries a flo8 mutation, and is only able to express the flocculin-encoding genes FLO1 and FLO11, when FLO8 ...

journal_title：Molecular microbiology

authors： Fichtner L,Schulze F,Braus GH

更新日期：2007-12-01 00:00:00