Abstract:
:A number of small, multicopy plasmids from Gram-positive bacteria replicate by an asymmetric rolling-circle mechanism. Previous studies with several of these plasmids have identified a palindromic sequence, SSOA, that acts as the single-strand origin (SSO) for the replication of the lagging-strand DNA. Although not all the SSOA sequences share DNA sequence homology, they are structurally very similar. We have used an in vitro system to study the lagging-strand replication of several plasmids from Gram-positive bacteria using the SSOA sequences of pT181, pE194 and pSN2 as representative of three different groups of Staphylococcus aureus plasmids. In addition, we have investigated the lagging-strand replication of the pUB110 plasmid that contains an alternative single-strand origin, SSOU. Our results confirm that RNA polymerase is involved in lagging-strand synthesis from both SSOA and SSOU-type lagging-strand origins. Interestingly, while initiation of lagging-strand DNA synthesis of pUB110 occurred predominantly at a single position within SSOU, replication of pT181, pSN2 and pE194 plasmids initiated at multiple positions from SSOA.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Dempsey LA,Zhao AC,Khan SAdoi
10.1111/j.1365-2958.1995.tb02377.xsubject
Has Abstractpub_date
1995-02-01 00:00:00pages
679-87issue
4eissn
0950-382Xissn
1365-2958journal_volume
15pub_type
杂志文章abstract::We have identified gene fusions of polyamine biosynthetic enzymes S-adenosylmethionine decarboxylase (AdoMetDC, speD) and aminopropyltransferase (speE) orthologues in diverse bacterial phyla. Both domains are functionally active and we demonstrate the novel de novo synthesis of the triamine spermidine from the diamine...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2011.07757.x
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1990.tb00652.x
更新日期:1990-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2007-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
abstract::Changes in the mRNA levels of two Mycobacterium tuberculosis genes (fbpB known as antigen 85B, and hspX known as Acr) were studied in infected human monocytes. Antigen 85B is an enzyme involved in cell wall biosynthesis and is also a major target of the immune response. Acr is a stress protein believed to be involved ...
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更新日期:2001-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mmi.13229
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2006.05477.x
更新日期:2006-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12211
更新日期:2013-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-02-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1046/j.1365-2958.1998.00645.x
更新日期:1998-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.tb02358.x
更新日期:1995-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.363898.x
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03908.x
更新日期:2004-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13440
更新日期:2016-09-01 00:00:00
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更新日期:2002-05-01 00:00:00
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更新日期:2008-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2958.1996.321396.x
更新日期:1996-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01632.x
更新日期:1999-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1990.tb02043.x
更新日期:1990-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2000.01923.x
更新日期:2000-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02356.x
更新日期:2001-04-01 00:00:00