Abstract:
:In Escherichia coli, RecA protein regulates the DNA damage-inducible survival-enhancing SOS response. Mutant allele recA730, which causes constitutive SOS expression, is lethal at high temperatures in B/r, a derivative of wild-type B, but not in K-12 or in certain B/r--K-12 hybrids. We present evidence that killing is due to SOS induction of a defective retronphage, phi R86, which is integrated into the B/r chromosome at 19 min, but is absent in K-12. phi R86 contains retron EC-86 which encodes reverse transcriptase and a small multicopy DNA-RNA complex, msDNA-RNA. Induction of phi R86 in recA730 B/r strains results in inhibition of host DNA replication before cell death. A retronphage 'killer' gene, ORF336, when overexpressed from a plasmid, causes similar effects without SOS induction. phi R86 is not detectably u.v.-inducible in recA+ strains.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Kirchner J,Lim D,Witkin EM,Garvey N,Roegner-Maniscalco Vdoi
10.1111/j.1365-2958.1992.tb01461.xsubject
Has Abstractpub_date
1992-10-01 00:00:00pages
2815-24issue
19eissn
0950-382Xissn
1365-2958journal_volume
6pub_type
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