Elongation factor Tu and E1 beta subunit of pyruvate dehydrogenase complex act as fibronectin binding proteins in Mycoplasma pneumoniae.


:The interactions between pathogenic bacteria and extracellular matrix (ECM) components markedly influence the initiation and establishment of infection. We have identified two surface proteins of virulent Mycoplasma pneumoniae with molecular masses of 45 and 30 kDa that bind to the ECM constituent, fibronectin (Fn). These Fn-binding proteins (FnBPs) were purified to near homogeneity using Fn-coupled Sepharose 4B-affinity column chromatography, and amino acid sequence analysis of the 45 and the 30 kDa proteins identified them as elongation factor Tu (EF-Tu) and pyruvate dehydrogenase E1 beta subunit (PDH-B) respectively. The genes for EF-Tu and PDH-B were cloned, and the entire EF-Tu gene and NH2-terminus of PDH-B (NPDH (pyruvate dehydrogenase E1 beta subunit from amino acid 1-244)-B) gene were overexpressed in Escherichia coli. The recombinant proteins, rEF-Tu and rNPDH-B, were purified to homogeneity by His-tag affinity column chromatography and used to immunize rabbits. Purified rEF-Tu and rNPDH-B bound to Fn using a ligand immunoblot assay and ELISA. Immunogold electron microscopy with polyclonal antibodies reactive against rEF-Tu (antirEF-Tu) and rNPDH-B (antirNPDH-B) and whole cell radioimmunoprecipitation (WCRIP) revealed the surface location of these proteins. Adherence of viable M. pneumoniae to immobilized Fn was inhibited by antirEF-Tu and antirNPDH-B antisera in a dose-dependent and cumulative manner. These results demonstrate that M. pneumoniae EF-Tu and PDH-B, in addition to their major cytoplasmic biosynthetic and metabolic roles, can be surface translocated, which confers additional important biological functions.


Mol Microbiol


Molecular microbiology


Dallo SF,Kannan TR,Blaylock MW,Baseman JB




Has Abstract


2002-11-01 00:00:00














  • Role of the ribosomal stalk components in the resistance of Aspergillus fumigatus to the sordarin antifungals.

    abstract::Aspergillus fumigatus, an important human nosocomial pathogen, is resistant to sordarin derivatives, a new family of antifungals that inhibit protein synthesis by interaction with the EF-2-ribosomal stalk complex. To explore the role of the A. fumigatus ribosome in the resistance mechanism, the fungal stalk proteins w...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Santos C,Ballesta JP

    更新日期:2002-01-01 00:00:00

  • Menaquinone synthesis is critical for maintaining mycobacterial viability during exponential growth and recovery from non-replicating persistence.

    abstract::Understanding the basis of bacterial persistence in latent infections is critical for eradication of tuberculosis. Analysis of Mycobacterium tuberculosis mRNA expression in an in vitro model of non-replicating persistence indicated that the bacilli require electron transport chain components and ATP synthesis for surv...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dhiman RK,Mahapatra S,Slayden RA,Boyne ME,Lenaerts A,Hinshaw JC,Angala SK,Chatterjee D,Biswas K,Narayanasamy P,Kurosu M,Crick DC

    更新日期:2009-04-01 00:00:00

  • CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.

    abstract::Stimulation of Escherichia coli with acetate elevates the acetylation level of the chemotaxis response regulator CheY. This elevation, in an unknown mechanism, activates CheY to generate clockwise rotation. Here, using quantitative selective reaction monitoring mass spectrometry and high-resolution targeted mass spect...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Fraiberg M,Afanzar O,Cassidy CK,Gabashvili A,Schulten K,Levin Y,Eisenbach M

    更新日期:2015-01-01 00:00:00

  • Regulation of the KstR2 regulon of Mycobacterium tuberculosis by a cholesterol catabolite.

    abstract::Cholesterol catabolism is widespread in actinobacteria and is critical for Mycobacterium tuberculosis (Mtb) virulence. Catabolism of steroid nucleus rings C and D is poorly understood: it is initiated by the CoA thioesterification of 3aα-H-4α(3'-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP) by FadD3, whose gen...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Casabon I,Zhu SH,Otani H,Liu J,Mohn WW,Eltis LD

    更新日期:2013-09-01 00:00:00

  • Post-transcriptional cross-talk between pro- and anti-colonization pili biosynthesis systems in Vibrio cholerae.

    abstract::The pathogen Vibrio cholerae modulates the expression of many genes in order to transition from its environmental reservoir to its niche in the human host. Among these are genes encoding two related Type IV pili, the mannose-sensitive haemagglutinin (MSHA) pilus, which aids V. cholerae persistence in aquatic environme...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hsiao A,Toscano K,Zhu J

    更新日期:2008-02-01 00:00:00

  • The RhaS activator controls the Erwinia chrysanthemi 3937 genes rhiN, rhiT and rhiE involved in rhamnogalacturonan catabolism.

    abstract::Erwinia chrysanthemi causes soft-rot diseases of various plants by enzymatic degradation of the pectin in plant cell walls. The linear regions of pectin are composed of an acidic sugar, D-galacturonic acid. The ramified regions of pectin also include neutral sugars, and are rich in L-rhamnose residues. E. chrysanthemi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hugouvieux-Cotte-Pattat N

    更新日期:2004-03-01 00:00:00

  • Regulation of plasmid R1 replication: PcnB and RNase E expedite the decay of the antisense RNA, CopA.

    abstract::The replication frequency of plasmid R1 is controlled by an unstable antisense RNA, CopA, which, by binding to its complementary target, blocks translation of the replication rate-limiting protein RepA. Since the degree of inhibition is directly correlated with the intracellular concentration of CopA, factors affectin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Söderbom F,Binnie U,Masters M,Wagner EG

    更新日期:1997-11-01 00:00:00

  • PerC and GrlA independently regulate Ler expression in enteropathogenic Escherichia coli.

    abstract::Ler, encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, induces the expression of LEE genes by counteracting the silencing exerted by H-NS. Ler expression is modulated by several global regulators, and is activated by GrlA, which is also LEE-encoded. Typical enteropathogenic...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bustamante VH,Villalba MI,García-Angulo VA,Vázquez A,Martínez LC,Jiménez R,Puente JL

    更新日期:2011-10-01 00:00:00

  • Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway.

    abstract::The energy requirement for the second step in pullulanase secretion by the general secretory pathway was studied in Escherichia coli. In order to uncouple the two steps in the secretion pathway (across the cytoplasmic and outer membranes, respectively) and to facilitate kinetic analysis of secretion, a variant form of...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Possot OM,Letellier L,Pugsley AP

    更新日期:1997-05-01 00:00:00

  • Multiple copies of MATE elements support autonomous plasmid replication in Aspergillus nidulans.

    abstract::The AMA1 sequence is an efficient plasmid replicator and transformation enhancer in Aspergillus nidulans. It comprises two long perfect inverted repeats (MATE elements) flanking a short, unique, central spacer. Subclone analysis indicates that the complete inverted duplication, but not the unique central spacer, is ne...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Aleksenko A,Gems D,Clutterbuck J

    更新日期:1996-04-01 00:00:00

  • Uropathogenic Escherichia coli use d-serine deaminase to modulate infection of the murine urinary tract.

    abstract::Although once thought to be unique to bacteria, d-amino acids are also produced by mammals. For example, d-serine is excreted in human urine at concentrations ranging from 3.0 to 40 micro g ml-1. An epidemiological survey demonstrated that urine isolates of E. coli are more likely to catabolise d-serine via expression...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Roesch PL,Redford P,Batchelet S,Moritz RL,Pellett S,Haugen BJ,Blattner FR,Welch RA

    更新日期:2003-07-01 00:00:00

  • A haem cofactor is required for redox and light signalling by the AppA protein of Rhodobacter sphaeroides.

    abstract::The AppA protein of Rhodobacter sphaeroides is unique in its ability to sense and transmit redox signals as well as light signals. By functioning as antagonist to the PpsR transcriptional repressor, it regulates the expression of photosynthesis genes in response to these environmental stimuli. Here we show binding of ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Han Y,Meyer MH,Keusgen M,Klug G

    更新日期:2007-05-01 00:00:00

  • Combining reverse genetics and nuclear magnetic resonance-based metabolomics unravels trypanosome-specific metabolic pathways.

    abstract::Numerous eukaryotes have developed specific metabolic traits that are not present in extensively studied model organisms. For instance, the procyclic insect form of Trypanosoma brucei, a parasite responsible for sleeping sickness in its mammalian-specific bloodstream form, metabolizes glucose into excreted succinate a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Bringaud F,Biran M,Millerioux Y,Wargnies M,Allmann S,Mazet M

    更新日期:2015-06-01 00:00:00

  • The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators.

    abstract::We report the characterization of the msbA gene, isolated as a multicopy suppressor of the HtrB temperature-sensitive phenotype. The msbA gene maps to 20.5 min on the Escherichia coli genetic map and encodes a protein with an estimated molecular mass of 64,460 Da, with the properties of an integral membrane protein. T...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Karow M,Georgopoulos C

    更新日期:1993-01-01 00:00:00

  • The antioxidant systems in Toxoplasma gondii and the role of cytosolic catalase in defence against oxidative injury.

    abstract::Superoxide dismutase, catalase, glutathione peroxidase and peroxiredoxins form an antioxidant network protecting cells against reactive oxygen species (ROS). Catalase is a potent H2O2-detoxifying enzyme, which is unexpectedly absent in some members of the Kinetoplastida and Apicomplexa, but present in Toxoplasma gondi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ding M,Kwok LY,Schlüter D,Clayton C,Soldati D

    更新日期:2004-01-01 00:00:00

  • Evidence that acetyl phosphate functions as a global signal during biofilm development.

    abstract::We used DNA macroarray analysis to identify genes that respond to the status of the intracellular acetyl phosphate (acP) pool. Genes whose expression correlated negatively with the ability to synthesize acP (i.e. negatively regulated genes) function primarily in flagella biosynthesis, a result consistent with observat...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wolfe AJ,Chang DE,Walker JD,Seitz-Partridge JE,Vidaurri MD,Lange CF,Prüss BM,Henk MC,Larkin JC,Conway T

    更新日期:2003-05-01 00:00:00

  • FbpA, a novel multifunctional Listeria monocytogenes virulence factor.

    abstract::Listeria monocytogenes is a Gram-positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. Signature-tagged mutagenesis (STM) was used to identify a gene named fbpA, required for efficient liver colonization of mice inoculated intravenously. FbpA was also shown to be requi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dramsi S,Bourdichon F,Cabanes D,Lecuit M,Fsihi H,Cossart P

    更新日期:2004-07-01 00:00:00

  • Genes in the accessory sec locus of Streptococcus gordonii have three functionally distinct effects on the expression of the platelet-binding protein GspB.

    abstract::Platelet binding by Streptococcus gordonii strain M99 is strongly correlated with the expression of the large surface glycoprotein GspB. A 14 kb chromosomal region downstream of gspB was previously shown to be required for the expression of this protein. The region encodes SecA2 and SecY2, which are components of an a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Takamatsu D,Bensing BA,Sullam PM

    更新日期:2004-04-01 00:00:00

  • Cell envelope components contributing to biofilm growth and survival of Pseudomonas putida in low-water-content habitats.

    abstract::Bacteria in terrestrial habitats frequently reside as biofilm communities on surfaces that are unsaturated, i.e. biofilms are covered in water films varying in thickness depending on the environmental conditions. Water availability in these habitats is influenced by the osmolarity of the water (solute stress) and by c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: van de Mortel M,Halverson LJ

    更新日期:2004-05-01 00:00:00

  • A gene involved in both protein secretion during growth and starvation-induced development encodes a subunit of the NADH:ubiquinone oxidoreductase in Myxococcus xanthus.

    abstract::The secretion of numerous proteins during vegetative growth of Myxococcus xanthus, and the multicellular development cycle induced upon starvation of these bacteria, are partially interrelated in so far as mutants impaired in extracellular protein production are unable to undergo development. We have cloned and sequen...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Laval-Favre K,Letouvet-Pawlak B,Friedrich T,Alexandre J,Guespin-Michel JF

    更新日期:1997-03-01 00:00:00

  • Molecular population genetic analysis of the enn subdivision of group A streptococcal emm-like genes: horizontal gene transfer and restricted variation among enn genes.

    abstract::The group A streptococcal emm-like genes, which encode the cell-surface M and M-like proteins, are divided into distinct mrp, emm and enn subdivisions and are clustered together in a region of the chromosome called the vir regulon. In order to understand the mechanisms involved in the evolution of emm-like genes, a 18...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Whatmore AM,Kapur V,Musser JM,Kehoe MA

    更新日期:1995-03-01 00:00:00

  • A network of net-workers: report of the Euresco conference on 'Bacterial Neural Networks' held at San Feliu (Spain) from 8 to 14 May 2004.

    abstract::In May 2004, over 100 bacteriologists from 19 different countries discussed recent progress in identification and understanding of individual signal transfer mechanisms in bacteria and in the mutual interactions between these systems to form a functional living cell. The meeting was held in San Feliu and supported by ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hellingwerf KJ

    更新日期:2004-10-01 00:00:00

  • Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism.

    abstract::Soupene et al. [J. Bacteriol. (2003) 185 5611-5626] made the unexpected observation that the presence of a mutation, in the gene for the N-acetylglucosamine repressor, nagC, increased the growth rate of Escherichia coli MG1655 on galactose, an unrelated sugar. We have found that NagC, binds to a single, high-affinity ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: El Qaidi S,Allemand F,Oberto J,Plumbridge J

    更新日期:2009-01-01 00:00:00

  • SecA is an intrinsic subunit of the Escherichia coli preprotein translocase and exposes its carboxyl terminus to the periplasm.

    abstract::SecA is the dissociable ATPase subunit of the Escherichia coli preprotein translocase, and cycles in a nucleotide-modulated manner between the cytosol and the membrane. Overproduction of the integral subunits of the translocase, the SecY, SecE and SecG polypeptides, results in an increased level of membrane-bound SecA...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: van der Does C,den Blaauwen T,de Wit JG,Manting EH,Groot NA,Fekkes P,Driessen AJ

    更新日期:1996-11-01 00:00:00

  • Mycobacterial protein antigens: a compilation.

    abstract::In response to recommendations from the Steering Committees responsible for co-ordination of World Health Organization programmes for research on the immunology of leprosy (IMMLEP) and tuberculosis (IMMTUB), a list was prepared summarizing the properties of mycobacterial proteins currently under investigation with res...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Young DB,Kaufmann SH,Hermans PW,Thole JE

    更新日期:1992-01-01 00:00:00

  • Mismatch repair and the regulation of phase variation in Neisseria meningitidis.

    abstract::Neisseria meningitidis controls the expression of several genes involved in host adaptation by a process known as phase variation. The phase variation frequency of haemoglobin (Hb) receptors among clinical isolates of serogroups A, B and C differed drastically, ranging from approximately 10(-6) to 10(-2) cfu-1. Freque...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Richardson AR,Stojiljkovic I

    更新日期:2001-05-01 00:00:00

  • In vitro characterization of constitutive CysB proteins from Salmonella typhimurium.

    abstract::Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Colyer TE,Kredich NM

    更新日期:1996-07-01 00:00:00

  • Disruption of msl3 abolishes the synthesis of mycolipanoic and mycolipenic acids required for polyacyltrehalose synthesis in Mycobacterium tuberculosis H37Rv and causes cell aggregation.

    abstract::Cell wall lipids of Mycobacterium tuberculosis containing multiple methylbranched fatty acids play critical roles in pathogenesis and thus offer targets for new antimycobacterial drugs. Mycocerosicacid synthase gene (mas) encodes the enzyme that produces one class of such acids. Seven mas-like genes (msls) were identi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dubey VS,Sirakova TD,Kolattukudy PE

    更新日期:2002-09-01 00:00:00

  • Transmembrane signal transduction by the Escherichia coli osmotic sensor, EnvZ: intermolecular complementation of transmembrane signalling.

    abstract::The Escherichia coli regulatory proteins, EnvZ and OmpR, are crucially involved in expression of the outer membrane proteins OmpF/OmpC in response to the medium osmolarity. The EnvZ protein is presumably a membrane-located osmotic sensor (or signal transducer), which exhibits both kinase and phosphatase activities spe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tokishita S,Mizuno T

    更新日期:1994-08-01 00:00:00

  • Short spacing between the Shine-Dalgarno sequence and P codon destabilizes codon-anticodon pairing in the P site to promote +1 programmed frameshifting.

    abstract::Programmed frameshifting in the RF2 gene (prfB) involves an intragenic Shine-Dalgarno (SD) sequence. To investigate the role of SD-ASD pairing in the mechanism of frameshifting, we have analysed the effect of spacing between the SD sequence and P codon on P-site tRNA binding and RF2-dependent termination. When the spa...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Devaraj A,Fredrick K

    更新日期:2010-12-01 00:00:00